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Biomedical subjects

X Huang

Publications and source records attributed to X Huang.

At least 253 records · Page 14Linked to original sources
PubMed2000

[A study of human breast carcinoma xenografts in nude mice].

OBJECTIVE: To study the spontaneous metastasis and the genetic stability of human breast carcinoma xenografts in nude mice and its micrometastasis. METHODS: The intact tissue of surgical specimens from breast carcinoma were xenografted into nude mice and transplanted from generation to generation. Cells from the xenografts were cultured in vitro and retransplanted into nude mice. Microsatellite DNA in genome of human breast carcinoma, xenotransplanted tumors and metastases in nude mice were analysed at three microsatellite loci. RESULTS: The tumorigenicity of orthotopic xenotransplantation was 88.6%(31/35) with a metastasis rate of 41.9%(13/31). Cells from the xenografts were cultured in vitro successfully, both the take rate of retransplantation into nude mice and the spontaneous lung metastasis rate were 100%(10/10). Microsatellite DNA sequences in genome of the xenotransplanted tumors and metastases in nude mice were identical with that of the original human breast carcinoma at the three microsatellite loci. CONCLUSIONS: The tumorigenicity and metastasis potential can be improved in human breast carcinoma xenograft using the intact fresh tumor tissue and orthotopic graft. The xenotransplanted tumors and their passaged tumors maintained the genetic stability. The detection of microsatellite DNA may discover micrometastasis in the nude mice model.

Animals↗
PubMed2000

[The promoting effects of mGM-CSF gene modified tumor vaccine immunization on peritoneal antigen presenting cells in mice].

OBJECTIVE: To investigate the effects of immunization with granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-modified tumor vaccine on the number and function of antigen presenting cells (APCs). METHODS: FBL-3 murine erythroleukemia cells were transfected with mGM-CSF recombinant adenovirus and then irradiated to prepare vaccine. The specific cytotoxic T lymphocyte(CTL) activity in vivo and survival of the mice i.p. immunized with this vaccine were observed. The percentage of 33D1+ dendritic cells(DCs) in peritoneal adherent cells were analyzed with flow cytometry. The number of peritoneal cell and function of peritoneal adherent cells of immunized mice were also investigated. RESULTS: High level of specific CTL activity were induced in the mice i.p. immunized with this vaccine and all of the mice were resistant to the subsequent challenge with the parental FBL-3 cells. The number of peritoneal APCs-increased. FACS analysis showed that the percentage of 33D1+ DCs in peritoneal adherent cells also increased. The allo-mixed lymphocyte reaction(MLR) stimulating activity of these APCs was enhanced. CONCLUSION: mGM-CSF gene-modified tumor vaccine has potent anti-tumor effect, and this may be related to the increased number and function of peritoneal APCs induced by mGM-CSF through paracrine mechanism at the vaccination site.

Animals↗
PubMed2000

Early and late long-term effects of vasectomy on Zn, Cd, and Cu levels in prostatic fluid and serum.

AIM: To evaluate the early and late long-term effects of vasectomy on the serum and prostatic fluid trace elements. METHODS: In 37 vasectomized and 25 non-vasectomized (control) men, the Zn, Cd and Cu levels in the serum and prostatic fluid were measured by means of inductively coupled plasma-atomic emission spectroscopy (ICP-AES). RESULTS: No significant difference was found in the prostatic Zn, Cd and Cu levels between the vasectomized and control group. The Zn level in the serum were significantly lower in the vasectomized men than in the controls (11.04 and 13.54 umol/L, respectively; P < 0.05), while the serum Cd and Cu levels were not significantly different between the two groups. CONCLUSION: Vasectomy may decrease the serum, but not the prostatic Zn levels. Its pathophysiological significance is worthy of further investigation.

Body Fluids↗
PubMed2000

Changes in genioglossus muscle activity in obstructive sleep apnea patients with and without snore guard.

OBJECTIVE: To study the effect of Snore Guard on the genioglossus (GG) muscle activity of obstructive sleep apnea (OSA) patients. MATERIALS AND METHODS: Fifteen male patients with mild to severe OSA were diagnosed by overnight polysomnographic studies, and were reexamined after using Snore Guard for 2-6 months. The difference in GG muscle activity was then compared. RESULTS: The overnight GG muscle activity decreased significantly with Snore Guard. The levels of GG muscle activity during awake quiet breath and obstructive sleep apnea were higher than in sleepstate quiet breath without Snore Guard, and significantly decreased with Snore Guard. The increase in GG muscle activity during sleepstate quiet breath with Snore Guard was not statistically significant. The fluctuating GG muscle activity without Snore Guard was effectively improved by treatment with the appliance. CONCLUSION: The effect of Snore Guard on OSA patients is a mechanical enlargement of upper airway volume. The genioglossus muscle is passive during treatment.

Adult↗
PubMed2000

Peroxynitrite leads to arteriolar smooth muscle cell membrane hyperpolarization and low vasoreactivity in severe shock.

This paper aimed to study the mechanism of vascular hyporeactivity during severe hemorrhagic shock. Rats were divided into control and shock group. Membrane potential of arteriolar strips was measured with intracellular recording method and membrane potential changes in arteriolar smooth muscle cells (ASMC) were recorded with membrane potential sensitive fluorescent dye (DiBAC4) and confocal microscopy. Hyperpolarization of ASMC membrane appeared at the late stage of shock, which correlated to low vasoreactivity. Glybenclamide, an inhibitor of K(ATP) channel reversed the hyperpolarizing effect. S-nitroso-N-acetylpenicillamine (SNAP), a donor of NO, in a higher concentration (400 mol/l) caused membrane hyperpolarization in control and shock group, which was completely reversed by application of Tiron, a scavenger of O2-. The hyperpolarizing effect of SNAP was decreased by ODQ, glybenclamide and (or) charybdotoxin. It is concluded that hyperpolarization of ASMC leads to vascular hyporeactivity. Peroxynitrite (OONO-) involves in the development of hyperpolarization in severe shock. The production of cGMP and activation of K(ATP) and K(Ca) channel contribute to the hyperpolarizing effect of OONO-*.

1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium ↗
PubMed2000

[Surgical resection of giant hemangioma].

OBJECTIVE: Through the operative experiences in 45 cases of giant hemangioma, it is elucidated that the operative procedure for hemangioma is an effective, radical method of treatment. METHODS: 1. Preoperative DSA can establish the diagnosis, delineate the range of lesion and facilitate the design of operation. 2. "Dissection over the false capsule" is applied in the operation. 3. The operative wound, is repaired with a plastic procedure. RESULTS: Of the 45 patients primary cure was obtained in 27, and secondary cure in 13 with effective rate of 89 percent. The followed-up was as long as 8 years with excellent results. CONCLUSIONS: The establishment of operative scheme is chiefly based on preoperative DSA, and the success is fundamentally determined by complete dissection over the so-called "false capsule" around the tumour during operation. This procedure can totally remove the tumour, eliminate recurrence and fulfil the purpose of radical resection, which is worthy of wide application.

Adult↗
PubMed2000

[Effect of initial population density on resting egg formation of rotifer Brachionus calyciflorus].

With the method of population accumulative culture, the effect of initial population density on the formation of resting eggs of freshwater rotifer(Brachionus calyciflorus), and on the rates of mictic female and fertilization was studied. During 11 days culture, the productivity and the formation efficiency of resting eggs, and the mean rate of mictic female were the biggest when the initial population density was 1.0-10.0 ind.ml-1, second by the density of 20.0 ind.ml-1, and the lowest by the density of 50.0 ind.ml-1. There was no significant effect of initial population density on the fertilization rate. Statistical analysis on maximum formation efficiency of resting eggs, their emergence and productivity showed that when the production of resting eggs was large-scaled proceeded, the feasible initial population density should be 10.0 ind.ml-1, and the corresponding time should be 6 days to obtain a higher yield of resting eggs and a higher formation efficiency.

Animals↗
PubMed2000

[Potentiation of arsenic trioxide-induced apoptosis by retinoic acid in retinoic acid sensitive and resistant HL-60 myeloid leukemia cells].

OBJECTIVE: To study the effect of arsenic trioxide (As2O3) on non-APL acute myeloid leukemia (AML) cells and the interreactive effect between retinoic acid (RA) and As2O3. METHODS: RA-sensitive (S) and RA-resistant (R) HL-60 non-APL AML cells were used as an in vitro model. Cell number and trypan blue were used to observe cell growth and survival. Apoptosis was determined by morphological changes, using a DNA laddering assay, terminal deoxynucleotidyl transferase (TdT) fragment end labeling assay and a flow cytometry assay. RESULTS: As2O3 induced apoptosis in both HL-60S and HL-60R cells, As2O3-induced apoptosis was both time- and concentration-dependent in a therapeutically-achievable As2O3 range (0.25-4.0 mumol/L). Both all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9cRA) potentiated As2O3-induced apoptosis, as measured by quantitative TdT fragment and labeling and flow cytometry assays in both HL-60S and HL-60R cells (P < 0.05, for all RA + As2O3 combinations vs As2O3 alone in both sublines). CONCLUSIONS: As2O3 may inhibit the growth of non-APL AML cells by promoting programmed cell death. RA can potentiate As2O3-induced apoptosis even in RA-resistant HL-60 cells in which the classical ATRA response pathway is repressed owing to a homozygous inactivating mutation in the retinoic acid receptor alpha. As2O3 can have clinical activity in non-APL cases of AML and the enhanced activity might result from the combined As2O3-RA therapy.

Alitretinoin↗
PubMed2000

Emodin on hepatic fibrosis in rats.

OBJECTIVE: To investigate effect of emodin on hepatic fibrosis in rats. METHODS: The rat hepatic fibrosis model was induced by the subcutaneous injection of 40% CCl4 (twice a week for 6 weeks) dissolved in olive oil. The emodin-treated rats were treated with low-dose, mediate-dose and high-dose emodin (20, 40 and 80 mg/kg body weight, once a day for 42 days) dissolved in 0.5% sodium carboxymethylcellulose (CMC), except receiving CCl4. Control group received only olive oil and 0.5% CMC. Liver functions were determined by standard procedure. Serum hyaluronic acid and laminin were determined by radioimmunoassay. Liver hydroxyprolines were determined. Histopathological changes were examined by optical microscopy. RESULTS: Compared with model group, the emodin-treated rats showed (1) liver functions were improved, alanine transaminase (ALT) and alkaline phosphatase (AKP) were obviously reduced, and total protein (TP) and albumin (ALB) were significantly increased; (2) serum hyaluronic acid and laminin were markedly reduced; (3) liver hydroxyproline was significantly decreased; (4) the degrees of fibrosis were reduced. The changes of parameters mentioned above were significant (P < 0.05 or P < 0.01). CONCLUSION: Emodin has effect on hepatic fibrosis in rats. The hepatoprotective of emodin may be one of mechanisms for liver fibrosis.

Animals↗
PubMed2000

[Effect of sijunzi decoction on gastric emptying rate in rat model of spleen deficiency syndrome].

OBJECTIVE: To observe the effect of Sijunzi decoction (SJZ) on gastric emptying rate in rat model of Spleen Deficiency Syndrome (SDS) and to explore the mechanism of "Yiqi Jianpi" (replenishing Qi and invigorating Spleen) effect with gastroduodenal myoelectrical activity and brain-gut peptide concentration. METHODS: After intramuscular injection of reserpine for 14 days, the rat model of SDS was established. Rats were randomly divided into 5 groups, group A treated by SJZ, group B treated by Liuwei Dihuang decoction (LWDH), group C naturally recovered, group D SDS rat model, consecutively treated for 14 days, and compared with group E, the normal control. Using radioisotopic technique to monitor 30 min gastric liquid emptying rate, and using the implanted bipolar silver electrode to observe the antral and duodenal myoelectrical slow wave, and the plasma and hypothalamus levels of motilium (MOT), cholecystokinin (CCK) and somatostatin (SS) were measured by radioimmunoassay (RIA) 14 days later respectively. RESULTS: Compared with the normal control, gastric liquid emptying rate of rat SDS model was lowered (P < 0.01, P < 0.05); the hypothalamus and plasma levels of MOT and SS reduced, both hypothalamus and plasma had higher CCK levels (P < 0.01, P < 0.05); in comparing with the controls, the rhythm, amplitude and motility index of myogastric slow wave were lower also. After treated with SJZ, the aforesaid data were normalized with insignificant difference in comparing with the normal control (P < 0.01), but the rat SDS model treated by LWDH and in natural recovery had no significant improvement. CONCLUSION: SJZ can promote gastric liquid emptying rate of rat SDS model by improving the myogastric electrical movement as well as regulating the levels of MOT, CCK and SS.

Animals↗
PubMed2000

[Abnormal P3 latency in obstructive sleep apnea syndrome, but normal in snore].

OBJECTIVE: To evaluate the P3 wave (latency, amplitude) in snorers and patients with obstructive sleep apnea syndrome (OSAS) and investigate the possible mechanism. METHODS: Three groups, OSAS patient group (n = 21), snorer group (n = 21) and control group (n = 21), were administered polysomnography (PSG), auditory evoked event-related potential (P3) and clinic memory test. The results were analyzed using GLM analysis and Post Hoc test. RESULTS: The auditory P3 latency in OSAS group was 363 ms +/- 22 ms (Fz), 369 ms +/- 28 ms (Cz), in snorer group 336 ms +/- 31 ms (Fz), 340 ms +/- 32 ms (Cz), and in control group 341 ms +/- 29 ms (Fz), 338 ms +/- 30 ms (Cz). There were significant differences between OSAS group and snorer group, as well as control group (P < 0.05). No significant difference was seen between snorer group and control group. Memory quotient (MQ) was reduced in snoren group as compared with control group. CONCLUSION: Abnormal auditory P3 latency indicates the cognitive dysfunction in OSAS patients. Nocturnal hypoxaemia may play an important role.

Adult↗
PubMed2000

[Screening for 6-pyruvoyl-tetrahydrobiopterin synthase (PTPS) deficiency: clinical analysis of 9 patients with PTPS deficiency].

OBJECTIVE: To show the incidence of 6-Pyruvoyl-tetrahydrobiopterin synthase (PTPS) deficiency among hyperphenylalaninemia and conclude the clinical outcome of patients with PTPS deficiency. METHODS: The urinary neopterin (N) and biopterin (B) were determined by HPLC in 69 PKU cases. BH(4) loading test and mutation analysis of PTPS were performed in patients who had abnormal urinary pterin patterns. Three patients with PTPS deficiency were placed on treatment with combined synthetic BH(4) and neurotransmitter precursors such as Dopa and 5-hydroxytryptophan. RESULTS: 9 out of 69 patients, whose urinary N/B exceed 38 [normal: 1.17 (1.4)] and B% were less than 5% [normal: 55.9 (18.6)], were diagnosed as having PTPS deficiency. Four kinds of PTPS gene mutations (P87S, N52S, D96N and G144R) were detected from 4 out of 9 cases with PTPS deficiency. The last mutation was a new mutation. The three typical PTPS-deficient patients had satisfactory physical development, their intelligence quotient (IQ) were 70 approximately 80 after treatment and one partial PTPSD had normal growth and mental development. CONCLUSION: The screening for BH(4) deficiency should be carried out among all patients with hyperphenylalaninemia in order to avoid misdiagnosis.

Biopterins↗
PubMed2000

[Study on perinatal group B Streptococcus carriers and the maternal and neonatal outcome].

OBJECTIVE: To investigate the group B streptococcus (GBS) colonization rate and the relationship between vaginal colonization of GBS and the maternal and neonatal outcome. METHODS: 3% TH selective broth media were used for culture of GBS. The vaginal samples were collected during different pregnant periods (before 20 weeks' gestation; 20-28 weeks; after 34 weeks' gestation) and other samples from different sites after labor (including neonatal throat, ear, umbilicus and placenta, umbilical blood, maternal blood, etc). RESULTS: The GBS carrier rate in 1 039 pregnant women was 11.07% (115/1,039). The colonization rate of different treating times was 29.73% (test three times), 15.50% (test twice) and 9.86% (test one time) respectively. The carrier rate of three-time-test was significantly higher than that of two times test (P < 0.01) and that of only one time (P < 0.01). The carrier rate in three periods was 16.84% (< 20 weeks), 18.40% (20-28 weeks) and 14.90% (> 34 weeks) respectively. Obstetric complications associated with GBS carrier were similar with those of non-carrier. Neonatal carrier rate was 9.95%. The neonatal carrier rate in the group of carrier mothers was higher than that of non-carrier mothers (14.29%:7.26%). The rate of neonatal pneumonia and the upper respiratory tract infection in GBS-carrier-mother was higher than that of non-carrier mothers (11.01%:9.71%; 8.27%: 6.07%) while it was similar in neonatal carrier group and non-carrier group (20.00%:14.92%; 10.00%:4.97%). CONCLUSION: GBS might be one of vaginal pathogens in Chinese women, but it is not severe enough to threaten the health of pregnant women and their newborns.

Carrier State↗
PubMed2000

[Treatment of ureteric lithioasis with ureteroscope: report of 234 patients].

SUBJECT: To sum up the experience in the treatment of ureteric lithioasis with ureteroscope. METHODS: From January. 1991 to November 1998 234 patients with ureteric lithiasis were treated with lithotripsy of ureteroscopy. RESULTS: 185 of 234 the patients (79%) were successfully treated with lithotripsy of ureteroscopy. Three of the patients (1.3%) had operative complications (ureterperforation 2 patients and ureteral mucosa avulsion in 1). CONCLUSIONS: Lithotripsy of ureteroscope is one of the effective methods in the treatment of ureteric lithiasis, especially in the lower part of ureters.

Adult↗
PubMed2000

[Damage to intra- and extrahepatic bile duct after hepatic artery embolization].

OBJECTIVE: To study the pathogenesis, treatment and prevention of bile duct damage arising from hepatic artery embolization. METHODS: Five patients with intra- and extrahepatic biliary damage resulting from hepatic artery embolization. Of these patients, 4 were due to hepatic artery embolization for hepatic haemangioma, and 1 was due to injecting TH (methacrylate) compound into the hepatic artery during operation. The hepatic artery was injected with ethanol so as to evaluate liver damage in experimental rats. RESULTS: All patients were found to have damage to intra- and extrahepatic bile duct at the hilum with biliary hepatocirrhosis; 4 of them received intrahepatic bile duct jejunal anastomosis and bile duct stent. Experiment result revealed obliteration of the bile duct and necrosis of the liver around the portal tract area. CONCLUSIONS: Arterial embolization of hepatic haemangioma may cause severe biliary damage.

Adult↗
PubMed2000

[Chemical-induced polycyst with renal tumor and expression of 8-OHdG in kidney tissue].

OBJECTIVE: To induce the rat model of polycyst with renal tumor and investigate the expression of 8-OHdG in the kidney tissues. METHODS: The rat model of polycyst with renal tumor, similar to human acquired cystic disease of the kidney (ACDK), was induced by oral administration of 2-amino-4, 5-diphenylthiazole (DPT) and N-nitrosomorpholine (NNM). Immunohistochemical method (LSAB) was used to assay the expression of 8-hydroxydeoxyguanosine (8-OHdG) in the rat kidney tissue. RESULTS: Three of 10 rats in the NNM group had renal solid adenomatous lesions. Bilateral polycysts were observed in all 9 rats of the DPT/NNM group. Seven of the 9 rats had cystic multistage renal tumor. In the DPT/NNM group, 4 rats had cystic adenomatous lesions, but none in the other groups showed this lesion. In the model, adenomatous lesions derived from polycysts in the rats were closely consistent to human ACDK in morphology. The significant expression of 8-OHdG was found on renal tubular cell, cystic epithelial cell, stromal cell and tumor cell in all rats of the NNM and DPT/NNM group. CONCLUSION: A rat model of polycysts with renal tumor, similar to human ACDK, induced by DPT and NNM provides evidences for further study on pathogenic mechanism in human ACDK with renal cell carcinoma. The expression of 8-OHdG, a DNA damage marker, in the renal tissues of rat model might help to explain the mechanism of cysticogenesis and carcinogenesis in human ACDK.

8-Hydroxy-2'-Deoxyguanosine↗
PubMed2000

[Predictive factors for successful sperm recovery in azoospermia patients].

OBJECTIVE: To find any predictive factors for successful testicular sperm recovery in azoospermic patients. METHODS: 50 cases of azoospermia were subjected to testicular biopsy. Serum follicle stimulating hormone (FSH), concentration, testicular volume, testicular histopathology, and testicular sperm extraction (TESE) were determined in each cases. RESULTS: Neither serum FSH values nor testicular volume were predictive of spermatozoa in TESE. Testicular histopathology was specific for predicting successful sperm recovery (sensitivity 96%; specificity 100%; accuracy 100%). CONCLUSIONS: Azoospermia patients with high FSH and small testis should be recommended for testicular biopsy and TESE to confirm if there is any testicular spermatozoa.

Adult↗