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Biomedical subjects

X Fu

Publications and source records attributed to X Fu.

At least 109 records · Page 6Linked to original sources
PubMed1997

Basic fibroblast growth factor reduces the gut and liver morphologic and functional injuries after ischemia and reperfusion.

OBJECTIVE: To explore the possible effects of basic fibroblast growth factor (bFGF) on ischemic gut and liver injuries after trauma. METHODS: Animal models of superior mesenteric artery occlusion (45 minutes) and reperfusion (3 days) were used in this study. Seventy-two Wistar rats were divided into three groups of 24 rats each. The animals in bFGF-treated group were injected with 4 microg bFGF/rat in 0.15 mL normal saline solution containing heparin 0.1% (w/v) through the jugular vein at the onset of reperfusion. In the normal saline control group, all rats received the same vehicle, but without bFGF. Group 3 (sham-operated) underwent the same laparotomy procedure, but without superior mesenteric artery occlusion. Liver function parameters, the levels of serum tumor necrosis factor alpha, nitric oxide, superoxide dismutase, malondialdehyde (MDA), tissue bacterial examination, and pathologic study were used to evaluate the results. RESULTS: In bFGF-treated rats, the amounts of serum alanine transaminase and aspartate aminotransferase and serum tumor necrosis factor-alpha were reduced significantly at 6, 24, and 48 hours when compared with normal saline-treated rats. However, the changes in nitric oxide, superoxide dismutase, and MDA varied from each other as a function of time after injury. The amounts of nitric oxide were increased significantly at 6 hours in intestine in normal saline-treated rats and in liver in bFGF-treated rats (p < 0.05). At 6 hours after reperfusion, the activity of superoxide dismutase in normal saline-treated rats were much lower in liver than those in bFGF-treated and sham-operated rats (p < 0.05), but the levels of MDA were increased in intestine in bFGF-treated rats and in liver in normal saline-treated rats when compared with sham-operated rats (p < 0.05). At 24 hours, the levels of MDA in normal saline-treated rats were much higher than those in both bFGF and sham-operated rats (p < 0.05). Bacterial examination revealed that the ratio and the amounts of bacterial translocation from gut to liver, spleen, and mesenteric lymph nodes in bFGF-treated rats were much lower than those in normal saline-treated rats. The results of pathologic study support the assumption that bFGF provided protective effects against reperfusion injury. CONCLUSIONS: Intravenous administration of bFGF may benefit in reducing gut and liver injuries after ischemia and reperfusion. The mechanisms of those effects may involve mitogenic and nonmitogenic effects of bFGF.

Animals↗
PubMed1997

E2a-Pbx1 induces aberrant expression of tissue-specific and developmentally regulated genes when expressed in NIH 3T3 fibroblasts.

The E2a-Pbx1 oncoprotein contains the transactivation domain of E2a joined to the DNA-binding homeodomain (HD) of Pbx1. In mice, E2a-Pbx1 transforms T lymphoblasts and fibroblasts and blocks myeloblast differentiation. Pbx1 and E2a-Pbx1 bind DNA as heterodimers with other HD proteins whose expression is tissue specific. While the transactivation domain of E2a is required for all forms of transformation, DNA binding by the Pbx1 HD is essential for blocking myeloblast differentiation but dispensable for fibroblast or T-lymphoblast transformation. These properties suggest (i) that E2a-Pbx1 causes cellular transformation by activating gene transcription, (ii) that transcription of E2a-Pbx1 target genes is normally regulated by ubiquitous Pbx proteins and tissue-specific partners, and (iii) that DNA-binding mutants of E2a-Pbx1 activate a subset of all gene targets. To test these predictions, genes induced in NIH 3T3 fibroblasts by E2a-Pbx1 were identified and examined for tissue- and stage-specific expression and their differential abilities to be upregulated by E2a-Pbx1 in NIH 3T3 fibroblasts and myeloblasts and by a DNA-binding mutant of E2a-Pbx1 in NIH 3T3 cells. Of 12 RNAs induced by E2a-Pbx1, 4 encoded known proteins (a J-C region of the immunoglobulin kappa light chain, natriuretic peptide receptor C, mitochondrial fumarase, and the 3',5'-cyclic nucleotide phosphodiesterase, PDE1A) and 5 encoded new proteins related to angiogenin, ion channels, villin, epidermal growth factor repeat proteins, and the human 2.19 gene product. Expression of many of these genes was tissue specific or developmentally regulated, and most were not expressed in fibroblasts, indicating that E2a-Pbx1 can induce ectopic expression of genes associated with lineage-specific differentiation.

3T3 Cells↗
PubMed1997

[The expression of proliferating cell nuclear antigen in breast phylloides cystosarcoma and its significance].

OBJECTIVE: To investigate the expression of proliferating cell nuclear antigen (PCNA) in breast phylloides cystosarcoma and its clinicopathological signification. METHODS: Immunohistochemistry (SP method) with monoclonal antibodies PC10 against PCNA was performed in 100 cases of phylloides cystosarcoma and 39 cases of adenofibroma. RESULTS: The positive rate of PCNA in phylloides cystosarcoma was 86%. The average PCNA index (PI) of phylloides cystosarcoma was significantly different among every histologic grading (F = 85.33, P < 0.01). The degree of differentiation was lower, the PI was higher. The PI was closely correlated with histologic grading (r's = 0.77). The PI in grade I phylloides cystosarcoma was higher than that in the group of adenofibroma with abundant mesenchymal cells (t = 3.42, P < 0.01). There was only a low correlation between PI and mitotic figures in phylloides cystosarcoma (r = 0.39). CONCLUSIONS: These findings suggest that the detection of PCNA has considerable practical value in reflecting the proliferation of activity of phylloides cystosarcoma, assisting the pathologists to make histologic grading; distinguishing the malignancy from benign ones (differential diagnosis) and evaluating the prognosis.

Adenofibroma↗
PubMed1997

[Uterine activity in active phase].

This study was designed to understand the uterine activity (UA) in normal labor. 43 nulliparous women(33 in normal course of labor and 10 in abnormal course of labor) were in the active phase of spontaneous labor with cephalic presentation for vaginal delivery. An open-end fluid filled intrauterine catheter connected to a pressure transducer was used to measure the uterine contraction intensity, frequency and duration. UA was quantified by means of Montevideo Unit (MU) and Uterine activity integral(UAI). The receiver operator characteristic(ROC) curve defined the cut-off points of UA between normal labor and abnormal labor. The results showed that MU and UAI were correlated with the rate of cervial dilatation in the active phase of labor (r = 0.3734 and 0.3502 respectively, P < 0.05). There was a strong correlation between MU and UAI (r = 0.7173, P < 0.001). The mean of UA in normal labor as MU = 226(s = 68.45) mmHg/15 min. UAI = 901(s = 258.02) kPa/15 min. The minimum of UA to normal labor in MU was 170 mmHg/15 min, of UAI 650 kPa/15 min. The study suggests that quantification of UA is very useful in the management of labor, especially in the proper use of oxytocin in cases abnormal labor.

Female↗
PubMed1997

[Immunohistochemical study of c-erbB-2 and p53 protein in breast papillomatosis and its malignant transformation].

OBJECTIVE: To evaluate the levels of c-erbB-2 and p53 protein in different stages of papillomatosis of breast and its malignant transformation and value for early diagnosis. METHODS: Levels of c-erbB-2 and p53 protein were detected by immunohistochemistry in 184 paraffin-embeded blocks. RESULTS: c-erbB-2 protein expression was seen in all stages of papillomatosis with different degrees of proliferation and in tissues undergone malignant transformation. The rate of overexpression increased with the degree of proliferation. The p53 protein expression was not found in papillomatosis with mild to severe proliferation. It was only overexpressed in 3.5% of carcinoma arising from papillomatosis, but increased to 25% when invasion occurred. CONCLUSION: c-erbB-2 assay may be helpful for detection of early malignant change of the highly proliferative papillomatosis, especially when histopathological evidence of malignancy is still lacking. p53 protein is of no significant diagnostic value in these lesions.

Breast Neoplasms↗
PubMed1997

[Pathological study of en bloc breast sections in papillomatosis and its malignant transformation].

OBJECTIVE: To investigate the characteristics of breast papillomatosis, its malignant transformation and relation with main ductal papilloma. METHODS: A histopathological study by en bloc breast subserial sections was performed on 14 cases of highly proliferative papillomatosis and 22 cases of carcinoma arising from the highly proliferative papillomatosis. RESULTS: The two types of lesions were widely scattered over the sections, covering more than two quadrants in 71.4% (10/14) and 63.3% (14/22) of the breast, respectively. Crisscross and histological transition of the two lesions were observed frequently in the sections of carcinoma arising from the highly proliferative papillomatosis. These two kinds of lesion often coexisted with main ductal papilloma. CONCLUSION: Papillomatosis is a breast precancerous lesion and it may have similar pathogenetic mechanism as main ductal papilloma.

Breast Neoplasms↗
PubMed1997

Gallstones and their chemical types in relation to serum lipids and apolipoprotein levels.

OBJECTIVE: To determine the profile of lipidemia in patients with gallstones and the characteristics of lipidemia in different kinds of gallstone in gallbladder for predicting high risk subjects predisposed to gallstone formation by means of some serum parameters. METHODS: Serum lipids and apolipoproteins levels in 47 patients with stone in gallbladder (stone group) were compared with those in 19 inpatients without stone (control group). The characteristics of lipidemia in different kinds of gallbladder stones were also compared. RESULTS: Serum apolipoprotein (Apo) A1, C2 and E levels in the stone group significantly increased as compared with the control group (P < 0.01), but there were no statistically significant differences in TC, TG, LDL-C, HDL-C, HDL-C/TC, LDL-C/HDL-C, Apo A2, B and C3 and Apo A1/Apo B levels between the stone and control groups (P > 0.05). Increased levels of serum LDL-C and Apo B and the LDL-C/HDL-C ratio (P < 0.05) were characterized as an index of cholesterol stones, while elevated levels of serum TG and Apo C2 (P < 0.05) as an index of pigment stones. CONCLUSIONS: Serum apolipoproteins might be more sensitive parameters as compared with serum lipids in distinguishing patients with stones from subjects without stones. There are different profiles of serum lipids and apolipoproteins in different chemical types of gallbladder stones.

Adult↗
PubMed1997

Percutaneous transluminal coronary angioplasty for acute myocardial infarction: initial experience in 43 cases.

OBJECTIVE: To evaluate the initial experience with the use of percutaneous transluminal coronary angioplasty (PTCA) in the set of acute myocardial infarction (AMI). METHODS: Between October 1993 and April 1996, PTCA was performed in 43 patients (34 male, 9 female) with initial onset of AMI. These patients ranged in age from 40 to 74 years (mean +/- SD, 60.3 3 +/- 9.2). Primary PTCA was performed in 16, rescue PTCA in 8, and semi-elective PTCA in 19 patients. RESULTS: The initial success was achieved in 95.3% (41/ 43). Failed angioplasty occurred in 2 patients, with acute reocclusion after rescue PTCA (this patient died of cerebral stroke 3 days later) in one patient, and small local intimal dissection during semi-elective PTCA in another patient. CONCLUSION: PTCA was a safe and effective approach in the treatment of AMI in the present study patients, with a high primary success and low acute complication rate.

Adult↗
PubMed1997

[Effects of bFGF on succinate dehydrogenase level and oxygen consumption of skin flap in rats].

To investigate the effects of basic fibroblast growth factor (bFGF) on necrosis rate, succinate dehydrogenase level and oxygen consumption of the skin flap, 18 Wistar rats were divided into 2 groups. Caudally based skin flap was raised on the back of each rat. Nine micrograms bFGF or normal saline with heparin was instilled under the flaps respectively after closure of the wounds. After 7 days, the necrosis rate of each wound was measured. The result showed that in bFGF group, the average necrosis rate of skin flap was 18.2%, less than that of the control group (37.14%). Succinate dehydrogenase content and oxygen consumption in bFGF group were higher than those of the corresponding sites in the control group (P < 0.05). It was suggested that the use of bFGF resulted in the decrease rate of necrosis of skin flap, and it maintained higher succinale dehydrogenase level and oxygen consumption. It was concluded that bFGF would probably be valuable for clinical use.

Animals↗
PubMed1996

Effects of progesterone and oxytocin on intracellular elemental composition of term human myometrium in vitro.

OBJECTIVE: To study the effects of progesterone and oxytocin on contractile activity related to the intracellular elemental composition of human pregnant myometrium. STUDY DESIGN: Myometrial strips were mounted in tissue baths and superfused with plain buffer, oxytocin or progesterone. Progesterone was initially added to the tissue bath or at the onset of spontaneous contraction of the strips. The myometrial contractile activities were recorded isometrically and the results were analyzed by a specific computer program. The effects of oxytocin and progesterone on the intracellular elemental composition were studied by X-ray microanalysis in an electron microscope. RESULTS: The contractile activity in terms of frequency and tonus of the muscle strips was increased by oxytocin and progesterone. This increase was more pronounced if progesterone was added to the bath at the start of the experiment. After 120 min of incubation with oxytocin and progesterone the total intracellular concentration of calcium was significantly higher (P < 0.0001 and P < 0.002, respectively) compared to buffer. CONCLUSIONS: (i) Oxytocin increases total intracellular calcium concentration concomitant with an increase in uterine contractile activity. (ii) Progesterone increases the myometrial tone and frequency of contractions, simultaneously with an increase in total intracellular calcium.

Calcium↗
PubMed1996

Progesterone, oestradiol and oxytocin and their in vitro effect on maintaining the number of gap junction plaques in human myometrium at term.

Previous studies by electron microscopy and laser confocal microscopy of immunohistochemically stained sections have shown that during pregnancy the extent of gap junction formation in human myometrium is low, but that an increase occurs in the active stages of labour despite a high concentration of progesterone in maternal blood. The present investigation focused on the effect of in vitro exposure of isolated myometrial tissue to progesterone, oestradiol and oxytocin, on the number of gap junction plaques in human myometrium at term. Myometrial biopsies were obtained at term from 13 pregnant women who had an elective caesarean section in the 37th or 40th week of pregnancy. The biopsies were immersed immediately in Hepes buffer and buffer containing 0.5, 5.0 micrograms mL-1 of progesterone, and 0.1 micrograms mL-1 of oestradiol. The muscle biopsies were trimmed under a stereo microscope into strips along the bundles of smooth muscle cells and mounted in tissue baths, superfused with Hepes buffer supplemented with glucose (0.01 mM); subsequently the strips were exposed to buffer containing different concentrations of progesterone, oestradiol and oxytocin. Incubation of the muscle strips for 180 min resulted in a significant decrease of the number of gap junctions (P < 0.01). Neither oestradiol or oxytocin, alone or in combination, had a significant effect on the maintenance of the number of gap junctions. The progesterone concentration of 5.0 micrograms mL-1, combined with oxytocin, and with or without oestradiol has a significantly positive effect on the number of gap junction plaques in strips of human myometrium at term (P < 0.05 vs. buffer alone). The high concentration of progesterone in the superfusion medium during in vitro experiments may be responsible for the maintenance of high numbers of gap junction complexes in term human myometrium. This finding is of interest in the light of findings of persisting high progesterone levels in maternal blood during labour.

Estradiol↗