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Biomedical subjects

X Fu

Publications and source records attributed to X Fu.

At least 91 records · Page 5Linked to original sources
PubMed1999

Longithorols A and B, novel prenylated paracyclophane- and metacyclophane-type hydroquinones from the tunicate Aplidium longithorax.

The tunicate Aplidium longithorax collected from Palau contained two novel prenylated paracyclophane- and metacyclophane-type hydroquinones, longithorols A (1) and B (2), in addition to longithorones A-I. Longithorols A and B were very unstable and were therefore isolated as their more stable pentaacetate forms, 3 and 4, respectively. The structures of 3 and 4 were determined by spectral data, especially 2D NMR data.

Animals↗
PubMed1999

mAngiogenin-3, a target gene of oncoprotein E2a-Pbx1, encodes a new angiogenic member of the angiogenin family.

Angiogenins are proteins in the pancreatic ribonuclease superfamily that utilize their ribonuclease activity to induce formation of new blood vessels. Recently we identified a new member of the angiogenin gene family, mouse angiogenin-3, by virtue of its transcriptional activation in NIH3T3 fibroblasts coincident with transformation by the chimeric leukemia oncogene, E2a-Pbx1. Here we have isolated the cDNA encoding mouse angiogenin-3 and used it to produce the protein in E. coli. We demonstrate that mouse angiogenin-3 is a ribonuclease whose activity and specificity towards tRNA and dinucleotide substrates differ from those of mouse angiogenin or of mouse angiogenin-related protein, a non-angiogenic factor. Mouse angiogenin-3 induced angiogenesis in both the chicken embryo chorioallantoic membrane assay and the rat cremaster muscle. Electron microscopy revealed that endothelial cells within vessels induced by both mouse angiogenin-3 and mouse angiogenin contain fenestrations similar to those observed in endothelial cells from neovasculature induced by vascular endothelial growth factor and basic fibroblast growth factor. Mouse angiogenin-3 also induced other molecular events typical of rapidly proliferating endothelial cells, such as increases in rough endoplasmic reticulum, polysomes, and mitochondria.

3T3 Cells↗
PubMed1999

A double blind observation for therapeutic effects of the tong luo kai bi tablets on rheumatoid arthritis.

The therapeutic effects of the Tong Luo Kai Bi Tablets [symbol: see text] in 120 patients with rheumatoid arthritis were observed in this clinical trial by the randomized double blind method. Both the observed group and the control group each had 60 patients. In the observed group treated with the Tong Luo Kai Bi Tablets, 1 case (1.7%) was cured clinically, 27 cases (45.0%) improved markedly, 26 cases (43.3%) improved, with a total effective rate of 90.0%. In the control group treated with Rheumatic Semen Strychni Tablets [symbol: see text], no case was cured, 16 cases (26.7%) improved markedly, 33 cases (55.0%) improved, with a total effective rate of 81.7%. Statistical data showed the Tong Luo Kai Bi Tablets had much better therapeutic effectiveness clinically than the Rheumatic Semen Strychni Tablets (P < 0.05).

Adult↗
PubMed1999

[The changes in endogenous growth factors and their relation with wound healing].

OBJECTIVE: To explore the effects of endogenous growth factors on the formation of scars and ulcers after trauma or burn. METHODS: The amounts of endogenous epidermal growth factor (EGF), tumor necrosis factor(TNF) and nitric oxide (NO) in scar and the developing granulation tissues after trauma or burn were determined and their relation with the wound healing were studied. RESULTS: The results showed that EGF, TNF and NO could be found in all scars and the granulation tissues, but their concentration varied with different tissues, sex and age of patients. In male patients or youngsters, the amounts of EGF, TNF and NO in scars were much greater than those in granulation tissues. CONCLUSIONS: The results indicated that the growth factors are necessary for wound healing, but insufficiency or overproduction of the endogenous growth factors may lead to the development of chronic ulcers or scar formation after trauma or burn.

Adult↗
PubMed1999

[Different expressions of bFGF gene in three kinds of skeletal muscles].

OBJECTIVE: To explore the distribution characteristics of bFGF gene, bFGF protein and receptor in rat skeletal muscles. METHODS: bFGF gene, and bFGF protein and receptor in rat gastrocnemius muscles were localized by ABC immunohistochemical method, in situ hybridization and a special staining methods. RESULTS: Based on the results from the special staining, the fibers in rat gastrocnemius muscle could be divided into three types: type I, II a and II b. The positive expression of bFGF gene was only found in the cytoplasm of type I and type II a fibers. The immunohistochemical localization showed that bFGF protein and its receptor were found in the connective parts of muscle endomysium in fibers of type I and type II a. CONCLUSION: This is the first study to identify the different expressions of bFGF gene, bFGF protein and its receptor in rat skeletal muscles. The results will help to understand the action and the relationship between bFGF autocrine and the embryo development of skeletal muscle fibers.

Animals↗
PubMed1999

[Effect of exogenous p16 gene on the growth of wild-type p53 human lung adenocarcinoma cells].

OBJECTIVE: To study the effect of p16 gene on human adenocarcinoma cell lines and the posibility of exogenous p16 gene for gene therapy of lung cancer. METHODS: A p16 gene recombinant expression vector was constructed by cloning p16 cDNA into the pcDNA3 vector at the sites of BamH1 and Xho I, and was transferred by electroporation into p16 gene deficient human lung adenocarcinoma cell lines (A549 and H460) which expressed wild-type p53 gene. The p16 mRNA and protein expression of A549-p16 and H460-p16 cell was detected by Northern blots and immunocytochemistry, respectively. The cell growth properties and cell cycle pattern were aassessed by flow cytometry. RESULTS: The growth and colony forming efficiency of A549-p16 and H460-p16 cells were significantly inhibited compared with the controls. Flow cytometry showed A549-p16 and H460-p16 cells were arrested in G1 phase of cell cycle, and the percentage of cells in S phase and G2/M phase was decreased significantly. Tumorigenicity of the A549-p16 and H460-p16 cells in nude mice was greatly inhibited. CONCLUSION: The results show that human p16 gene can suppress the malignant phenotype of wild-type p53 human lung adenocarcinoma cell lines (A549 and H460). p16 gene can play a role in the gene therapy of human lung adenocarcinoma.

Adenocarcinoma↗
PubMed1999

[Clinical observation on effect of yiqi huoxue decoction in comprehensive treatment on advanced stage of esophageal cancer].

OBJECTIVE: To observe the effect of Yiqi Huoxue (Replenishing Qi and activating blood circulation, YQHX) decoction in patients with advanced stage esophageal cancer treated with radiotherapy (RT) and intervention chemotherapy (ICT). METHODS: In comparing 31 patients who were treated with RT and ICT (Group A), and 31 patients treated with RT and ICT plus YQHX decoction (Group B). RESULTS: Immediate effective rate were 48.4% in Group A and 64.5% in Group B (P > 0.05). The function of bone marrow in Group B was obviously higher than that in Group A. The 1 year metastasis rate in Group A was higher than that in Group B, but the long-term survival rate of Group B was obviously higher than that in Group A (P < 0.05). CONCLUSION: YQHX decoction could reduce the inhibition of bone marrow caused by radiotherapy and intervention chemotherapy, lower the metastasis rate, prolong the long-term survival rate and improve the quality of life.

Adult↗
PubMed1999

[Surgical treatment of the functional obstruction of eustachian tube of middle-aged and aging people].

OBJECTIVE: To expore the effect and the way of palatosapingeus tendon-shortening surgery to treat the functional obstruction of eustachian tube of middle-aged and aging people. METHOD: Seven cases of palatosapingeus tendon-shortening surgery were analyzed retrospectively. RESULT: After operations the symptoms of all cases were relieved in a week. The tympanogram returned to type "A". The audiogram showed that the airbone gap disappeared. The shape of tympanic membrance returned to normal state. None of them recured in one-three year following up. CONCLUSION: The effect of palatosapingeus tendon-shortening surgery is satisfactory so long as its surgical indication is correctly selected.

Aged↗
PubMed1999

[Migration behavior of low-mass anions in capillary electrophoresis].

The dependence of effective mobility on ionic equivalent conductivity for 33 anions was studied using 5 mmol/L sodium chromate and 0.5 mmol/L tetradecyltrimethylammonium bromide (TTAB) as background electrolyte. The relationship was developed between effective mobility mueff and carbon number of mono-carboxylic acid and dicarboxylic acid homologues. The results showed that the effective mobility of anions increased with increasing ionic equivalent conductivity. The 1/mueff was linearly related to the carbon number of mono-carboxylic acid (C2-C7) and dicarboxylic acid(C2-C10). The correlation coefficient was more than 0.997.

Anions↗
PubMed1998

The effects of the Rho-kinase inhibitor Y-27632 on arachidonic acid-, GTPgammaS-, and phorbol ester-induced Ca2+-sensitization of smooth muscle.

The effects of the Rho-kinase inhibitor, Y-27632 [1] on Ca2+-sensitization of force induced by arachidonic acid (AA), phorbol 12,13-dibutyrate (PDBu), GTPgammaS, and by the stable thromboxane analog, 9,11-dideoxy-9alpha,11alpha-methanoepoxy-PGF2alpha (U-46619), were determined in alpha-toxin-permeabilized smooth muscles. Y-27632 relaxed (up to 99%) Ca2+-sensitization by GTPgammaS (10 microM) and U46619 (1 microM), but not by PDBu (20 microM), and reduced GTPgammaS-induced myosin light chain (MLC20) phosphorylation from 28% to 17% (P=0.002). GTPgammaS-induced force sensitization was inhibited by Y-27632 more potently when the inhibitor was added during the plateau of force than prior to stimulation. In alpha-toxin-permeabilized smooth muscle, Y-27632 inhibited AA (50 microM)-induced Ca2+-sensitization of force (by 66 +/- 1.3%) and reduced MLC20 phosphorylation. In contrast, Y-27632 did not relax force Ca2+-sensitized by AA in smooth muscle permeabilized with Triton X-100. We conclude that (i) AA induces Ca2+-sensitization through dual mechanisms, one mediated by Rho-kinase (or a related kinase), and (ii) Rho-kinase is not required for phorbol ester-induced Ca2+-sensitization.

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗
PubMed1998

Randomised placebo-controlled trial of use of topical recombinant bovine basic fibroblast growth factor for second-degree burns.

BACKGROUND: Wound healing is a dynamic process that could be accelerated by growth factors. We investigated the effect of recombinant bovine basic fibroblast growth factor (rbFGF) on burn healing in a randomised placebo-controlled trial. METHODS: We recruited 600 patients with superficial or deep second-degree burns. Patients received 150 AU/cm2 daily topical rbFGF (n=300) or placebo (n=300) plus vehicle. We assessed healing by photography, punch-biopsy, and clinical examination. FINDINGS: All patients treated with rbFGF had faster granulation tissue formation and epidermal regeneration than those in the placebo group. Superficial and deep second-degree burns treated with rbFGF healed in a mean of 9.9 (SD 2.5) days and 17.0 (4.6) days, respectively, compared with 12.4 (2.7) and 21.2 (4.9) days (p=0.0008 and p=0.0003, respectively). No adverse effects were seen locally or systemically with rbFGF. INTERPRETATION: rbFGF effectively decreased healing time and improved healing quality. Clinical benefits would be shorter hospital stays and the patient's skin quickly becoming available for harvesting and grafting.

Administration, Cutaneous↗
PubMed1998

[DNA sequencing of human ciliary neurotropic factor gene by silver staining method].

OBJECTIVE: To introduce a non-radioactive protocol for DNA sequence analysis which employs a silver staining procedure METHODS: DNA template is amplified by Taq DNA polymerase to generate sequence ladder, No labeled nucleotide or primer is involved in the thermal-cycling sequencing reaction. A sensitive silver staining procedure is employed to visualize bands in sequencing gel. RESULTS: This silver staining method is used for DNA sequence analysis of human cilliary neurotrophic factor (hCNTF) gene. The resulted band resolution is comparable to radioactive sequencing method, and data can be obtain within less than 2 hours after sequencing reaction. CONCLUSION: Silver staining is a rapid, inexpensive and reliable method for DNA sequence analysis.

Ciliary Neurotrophic Factor↗
PubMed1998

Ischemia and reperfusion impair the gene expression of endogenous basic fibroblast growth factor (bFGF) in rat skeletal muscles.

Our previous studies showed that the amount of endogenous basic fibroblast growth factor (bFGF) was reduced after ischemia and reperfusion insult. One of the mechanisms involved in the decrease of endogenous bFGF is the increased destruction of this growth factor associated with oxygen free radical activation and inflammation. We hypothesized that the wounding also impairs the secretion of bFGF and examined the bFGF gene expression in skeletal muscles after ischemia and reperfusion insult. In this study, a rat leg ischemia (4 h) and reperfusion (24 h) injury model was prepared and the in situ hybridization method and reverse transcriptase polymerase chain reaction technique (RT-PCR) were used to evaluate the bFGF gene expression and its localization in control (normal) and injured rat skeletal muscles. The results showed that the bFGF mRNA expression was localized in the cytoplasm in control skeletal muscle, especially at the periphery inside the cells. According to the intensity of the stain, four main classes of fibers could be identified: strongly, moderately, weakly, and negatively stained fibers. Based on the positive stain, about 82% of the total fibers examined were positive for bFGF mRNA stain. In ischemic or ischemic and reperfused rat skeletal muscles, the localization of bFGF mRNA expression was similar to that in normal skeletal muscles, but only 52% in ischemic muscles and 22% in ischemic and reperfused muscles had positive bFGF mRNA staining. RT-PCR confirmed a significant decrease in bFGF mRNA expression in ischemic and reperfused rat skeletal muscles. These results suggest that the acute ischemia and reperfusion not only induce the destruction of endogenous bFGF molecule, which is stored at the extracellular matrix of the fibers, but also downregulate the bFGF gene expression. The simultaneous dysregulation of endogenous bFGF gene expression and decreased synthesis of bFGF protein suggest a possible role of this growth factor in delayed wound healing.

Animals↗
PubMed1998

Factors controlling activity of zirconia-titania for photocatalytic oxidation of ethylene.

Small photocatalytic devices were developed to remove ethylene from closed plant growth units flown in space. The devices utilized sol-gel-derived catalyst pellets of zirconia-titania. This study was undertaken to understand the significance of different factors on the photocatalytic activity of the catalyst. Increasing reaction temperatures and decreasing humidity of the air significantly increased oxidation of ethylene. The quantity of ethylene oxidized per unit time increased linearly with increasing flow rates, and increasing concentrations of ethylene. Zirconia-titania pellet size and heel depth had little effect on oxidation of ethylene. Platinizing the zirconia-titania significantly increased ethylene oxidation. The catalyst was found to absorb large quantities of water when the humidity of the air stream was elevated and this greatly decreased catalytic activity.

Air Conditioning↗