Developing a census data system in China.
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Asia↗
Biomedical subjects
W Zhang
Publications and source records attributed to W Zhang.
[Preparation of oral microspheres carrying V. cholera vaccine and its target's distribution].
To prepare oral biodegradable microspheres carrying V. cholera vaccine, the major outer membrane protein (OMP, MW = 41 kd) as a common antigen of cholera Vibriae was obtained from the classical strain Inaba 569 B, and the OMP was encapsulated in the biodegradable delivery system comprising Poly (DL-Lactide)-Co-Poly(ethylene glycol)microspheres. The average size of the microspheres was less than 5 microns, the amount of OMP encapsulated in microspheres was 15.3%. It was found that microspheres were taken up in Peyer's patches and then distributed in spleen, liver and mesenteric lymph nodes after oral administration.
Animals↗
[Distribution of nitric oxide synthase-positive neurons in the hypothalamus of cat].
NADPH-diaphorase histochemistry was used to study the distribution of Nitric Oxide Synthase (NOS)-Positive neurons in the hypothalamus of adult cat. NOS-positive neurons were observed in the paraventricular hypothalamic nucleus, suprachiasmatic nucleus, dorsomedial hypothalamic nucleus, perifornical nucleus, anterior hypothalamic area and lateral hypothalamic area. Two differences between the cat and rat were noticed. Firstly, NOS-positive neurons were observed in the suprachiasmatic nucleus of cat, but the neurons in that of rat were negative for NOS. Secondly, the neurons in supraoptic nucleus of rat exhibited an intensive reaction to NADPH-diaphorase, but those in that of cat were faintly visible.
Animals↗
[Separation and identification of flavonoid glucosides from Elsholtzia blanda (Benth.) Benth].
OBJECTIVE: To study chemical elements of the inflorescence of Elsholtzia blanda produced in the southwest of Yunnan. METHOD: Using chemical techniques and spectroscopic analysis. RESULT: Four flavonoid glucosides from the water extract of E. blanda were isolated and identified as isoastragalin(I), luteolin-7-glucoside(II), luteolin-3'-glucoside(III) and lutelin-7-galactoside(IV). CONCLUSION: All the four flavonoid glucosides were isolated from the plant for the first time.
Flavonoids↗
Pilot program sheds light on improved services.
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Asia↗
[Immunohistochemical study of basement membrane proteins expression in adenoid cystic carcinoma of salivary glands].
OBJECTIVE: To investigate the expression of the basement membrane proteins in adenoid cystic carcinoma (ACC) of the salivary glands and discuss the relationship between the expression of the proteins and the differentiation as well the prognosis of ACC. METHODS: 101 patients which were confirmed pathologically were collected and made a follow-up, and 30 cases of them were selected to detect the basement membrane proteins (type IV collagen, fibronectin, laminin and heparan sulfate proteoglycan) by the means of immunohistochemistry. RESULTS: The cribriform pattern of ACC had a more favorable prognosis than the solid pattern which had a lower rates of metastasis of/and recurrence. The positive locations and the degrees of the four proteins varied with the histological patterns of ACC. The cribriform pattern had the strongest positive expression and the solid pattern the weakest. In addition, the staining of fibronectin were weaker in all three patterns of ACC. CONCLUSION: The degree of the positive expression of the four proteins has a negative relation to the differentiation of the tumor cells. The lower degree of differentiation the tumor cells are, the weaker positive signals they express. Of the four substances in the basement membrane, laminin is the main material which adhere the parenchymal cell to the collagen in basement membrane of the tumor. Basement membrane proteins could be regarded as one of the basis to evaluate the prognosis of ACC.
Basement Membrane↗
[Telomerase hTRT mRNA expression in oral squamous cell carcinogenesis].
OBJECTIVE: To investigate the relationship between telomerase hTRT gene expression and malignant development of oral mucosa epithelium. METHODS: 82 cases were detected by in situ hybridization technique for hTRT gene, including 7 cases of normal oral mucosa, 7 cases of hyperplasia lesions, 30 case sof oral precancerous lesions (dysplasia lesion), 8 cases of oral mucosa carcinoma in situ and 30 cases of oral squamous cell carcinomas. RESULTS: 1. Weaker expression of hTRT mRNA was observed in normal oral mucosa and hyperplasia lesions. Positive cells were present in basal layer and parabasal layer, and hTRT positivity was 21.4% (3 of 14). 2. The expression of hTRT mRNA in the precancerous lesions was gradually strong associated with phenotypic progression and the degree of dysplasia. Positive cell extended to multi-layered epithelium, and hTRT positivity was 46.7% (14 of 30). 3. Strong hTRT mRNA expression was observed in squamous cell carcinoma, and the positivity was 81.6% (31 of 38). CONCLUSION: Telomerase hTRT gene expression is closely related to the malignant degree of oral mucosa, the reactivated telomerase may play a crucial role in the progression of oral cancer.
Carcinoma, Squamous Cell↗
[Study of effects of six caries-preventive agents on enamel decalcification].
OBJECTIVE: To compare the influence of 6 caries-preventive agents containing fluoride and trace element on dental enamel decalcification and observe the relationship between enamel decalcification and time of early carious lesions. METHODS: 84 bovine teeth were selected and divided into 7 groups. Experimental groups were treated with SrF, SnF2, (NH4)2 MoO2F4, ZnF, LaF and NaF with the same [F-]concentration and deionised water respectively, then all the specimens formed artificial carious lesion in lactic acid gel. RESULTS: The [Ca2+] concentration of lactic acid gel showed a variety of enamel decalcification among specimens treated with fluoride agents after 48 hours, on the other hand, all the specimens presented a platform of enamel decalcification after 48 hours. SnF2 demonstrated the strongest inhibition on enamel decalcification, meanwhile, NaF has the minimum influence on it. CONCLUSION: The time of forming artificial carious lesion with acid etch is about 48 hours, moreover, caries-preventive agents containing fluoride and trace element are effectively able to inhibit enamel decalcification, and the potential mechanism is the interaction of fluoride and trace element.
Animals↗
[Evaluation of methods of drug release rate in root canal disinfection controlled-release delivery system containing metronidazole].
OBJECTIVE: To measure the release rate of drug in controlled-release delivery system containing metronidazole. METHODS: Drug release tests in vitro, in vivo and through the roots of extracted teeth were performed. The release rate of metronidazole was calculated after 1 day, 3 days, 7 days and 10 days, and the results were statistically analyzed. RESULTS: Significant difference was observed among the total drug release rate in vitro, in vivo and through the roots of extracted teeth (P < 0.01). There was no statistically significant difference between the release test in vivo and through the roots of extracted teeth in vitro (P > 0.05). CONCLUSION: The method of using extracted teeth and the controlled release medium at 37 degrees C, pH7.4 was close to the situation in vivo, and it was an effective method to measure the drug release rate of the root canal disinfection controlled-release delivery system.
Anti-Infective Agents, Local↗
[Quantitative stereologic analysis of metabolize dynamic of protein and DNA during somatic embryogensis in Lycium barbarum L].
The protein and DNA content in somatic embryos of Lycium barbarum L were analysed using quantitative stereologicai procedure formed from Cell Morphometry. The photos were transformed into digital images by the Sharp JX-610 scanner. Then the quantity of protein and DNA was count applying digital images processing software. By the result we discuss the relationship between changes in protein and DNA metabolize dynamic and somatic embryogensis.
Culture Techniques↗
[The basic model and its peculiarity of relaxation theory in chromatography].
According to the basic idea of relaxation theory in chemical dynamics and the moving character of the sample molecules in a chromatographic column, a new transfer model was established. It was supposed that solute molecules were in equilibrium between the stationary phase and mobile phase, and transferred in the mobile phase by a way of jumping. The generating function was used to investigate the non-continuous dynamic transfer differential equations, and the basic profile of solute's distribution in the column is gained [formula: see text] Based on this equation, the effect of transfer coefficient on the profile had been explained. The results show that relaxation time is an important factor which is related to the capacity factor and diffusion coefficient, and the number of jumping is a similar parameter to the plate number. The digital calculation method was used to study the influence of different physical parameters on the profile. The continuous form of the profile was also compared with the one from other methods.
English Abstract↗
[The effect of organic modifier on the capacity factor of the solute in the micellar electrokinetic capillary chromatography].
In this paper the mass action law has been used to study the relationship of solute, organic modifier and micelle in the micellar electrokinetic capillary chromatography. A series of equations were given showing the relationship of capacity factor(k) of the solute, equivalent constant, concentration of solute and migration speed under the condition of using only one organic modifier. The results showed that the organic modifier can, to some extent, be dissolved in micelles. It has almost no effect on micellar actual migration speed, but, the organic modifier influences the CMC of surfactants and the distribution of solutes between two phases and results in the change of the capacity factors of the solutes. No simple equations like those in HPLC can be used to show the effect of organic modifier and surfactants on k. At the first approximation, they have the following equation, lnk approximately a + bCB + clnCB where, CB is the total concentration of organic modifier, a, b and c are constants.
English Abstract↗
[Progress in technique of computer for three dimensional reconstruction from biological tissue series slices images].
The development of the technique of computer processing images and biotechnology from late 80's to early 90's has rovided the possibility of displaying three dimensional images of biological tissues truly. Now the technique of computer for three dimensional reconstruction is more and more widely used in the biological territory. This paper introduces the progress in the technique of computer for three dimensional reconstruction from biological tissue series slices images. Emphasis is laid on registration, reconstruction & display, image data compressing and image automatic segmentation.
Algorithms↗
[Determination of C1-C5 composition of liquefied petroleum gas by capillary gas chromatography].
In this paper, thirteen C1-C5 hydrocarbons in liquefied petroleum gas were separated and determined by using capillary gas chromatography. The cloumn used was 50 m x 0.53 mm i.d. HP PLOT-Al2O3. A standard mixture of known composition was used for the determination of correction factots and the corrected area mormalization was used for quantification. The relative standard deviations (n = 5) were 0.39%-5.4%. Ethane, ethylene and butene were separated to baseline.
English Abstract↗
[Rapid determination of saccharin sodium in ice cream by high performance liquid chromatography (HPLC)].
This is a method for the rapid determination of saccharin sodium in ice cream by HPLC. A radial compression C18 cohumn, 100 mm x 8 mm i.d., and ultraviolet detector at 220 nm were applied to the determination without extraction. The solution of samples would be determined after they were treated with the solution of copper sulfate and sodium hydroxide in water. The detectable amount was 0.38 mg/kg, the coefficient of variation CV was 1.72%, and the recovery was 96.25%-101.75%.
Chromatography, High Pressure Liquid↗
[The study of carbonaceous adsorbent for solid-phase microextraction and the comparison with commercial device].
The parameters governing solid-phase microextraction have been investigated, and the development of carbonaceous adsorbent for SPME is presented because it is durable and inexpensive. The time profile curves were determined for several organic pesticides having a wide range of water solubilities and the effect of salt was also investigated. The method of SPME-GC-ECD to analyse trace level of the organic pesticide in water is established by the determination of optimum parameters. The comparison with commercial SPME device shows that the carbonaceous adsorbent having been treated with physical or chemical method for SPME can get lower limits of detection than polyacrylate and polydimethylsiloxane, although there are still some work to be done to improve its precision. The results shows that this new SPME device has great application potential.
Acrylic Resins↗
[Fraction P isolated from the ribosomes of Serratia odorifera and its tumor inhibiting effect].
A protein(component P) with molecular weight of 16 kD was isolated by the method of preparation electrophoresis from the ribosomes of Serratia odorifera, which were obtained by the method of differential centrifugation. This protein showed specific immune response reactivity with the antiserum of the ribosomes mentioned above. Meanwhile, component P showed apparent protective function for QGY-tumorcharged nude mouse and different ways of giving medicine. It was evident from study results that component P isolated from the ribosomes of Serratia odorifera showed a direct function for tumor growth inhibition and it was the effective component of the ribosomes of Serratia odorifera.
Animals↗
[Detection of heterophyid trematode egg in the blood of a case].
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Aged↗