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Biomedical subjects

W Wagner

Publications and source records attributed to W Wagner.

At least 235 records · Page 13Linked to original sources
PubMed1987

Enzymology of Fructan Synthesis in Grasses: Properties of Sucrose-Sucrose-Fructosyltransferase in Barley Leaves (Hordeum vulgare L. cv Gerbel).

Fructan synthesis was induced in excised primary leaf blades of Hordeum vulgare L. cv Gerbel by illumination in 30 millimolar fructose. This treatment induced a 26-fold increase of sucrose-sucrose-fructosyltransferase (SST, EC 2.4.1.99) activity within 24 hours. Acid invertase (EC 3.2.1.26) activity remained about constant. By preparing protoplasts from induced leaves, approximately 80% of the invertase activity was removed with the cell walls while SST was retained. The protoplast homogenate was used to partially purify and characterize SST. Acid precipitation (pH 4.75) and anion exchange chromatography (fast protein liquid chromatography on Mono ;Q') resulted in a recovery of about 80% of total SST activity. The principal activity (SST 1), accounting for 85% of the activity recovered, was purified about 200-fold. It was essentially free of invertase activity and catalyzed the synthesis of a trisaccharide which co-chromatographed with isokestose (1F-beta-fructosylsucrose). The remaining 15% of SST activity (SST 2) was purified about 35-fold. It retained substantial invertase activity and catalyzed the synthesis of only one trisaccharide which co-chromatographed with kestose (6F-beta-fructosylsucrose). It is concluded that barley leaves which store mainly fructan of the phlein type (beta-2-6 polyfructosylsucrose), nevertheless contain sucrose-sucrose 1F-beta-d-fructosyltransferase as the key enzyme of fructan synthesis.

Journal Article↗
PubMed1987

[Esophageal diverticulum--indications for surgical therapy and late results].

63 cases of oesophageal diverticulum were analysed, and surgical therapy was chosen for 45 of them. Most of them were located in the cervical region of the oesophagus (44.4 per cent). Cervical and intrathoracic diverticula or those causing pain and possible bleeding were indications for surgical treatment. No postoperative complications occurred to the above patients. Recurrence was observed in three of 28 cases, following surgery for Zenker's diverticulum. No complaints at all were recordable from 79 per cent of the patients in follow-up checks after surgery.

Adult↗
PubMed1987

[Clinical aspects and therapy of Kaposi's sarcoma].

Three own cases are presented in order to discuss the clinical characteristics as well as the therapy of the Kaposi syndrome. This disease occurs very rarely. In its typical form it has to be classified as semi-malignant. Generally it takes an extended course over many years. In case of loco-regional tumors, radiotherapy is considered to be the method of choice. It should be performed in an extended-field technique allowing to reduce the recurrence rate and to achieve a better local tumor control. The total dose is individually determined between 25 and 40 Gy.

Adult↗
PubMed1987

[Present status of thymoma therapy].

Eight patients with histologically verified thymoma were treated between 1962 and 1985 at the Radiologic Hospital of the University of Münster. All patients were submitted to thoracotomy and subsequent irradiation. A primary irradiation was performed in four cases. The average survival time of the only irradiated patients was 8.5 months. A considerably better average survival time, i.e. 46 months, was reached by surgical procedure with postoperative irradiation. The therapy of choice in the treatment of malignant thymomas is radical operation and subsequent radiotherapy; in case of benign thymomas a postirradiation is not necessary. 25% of our patients showed early recurrences, parathymic syndromes, and hematogenous metastases. Lymphogenic metastases were not observed. Chemotherapy exerted no demonstrable influence on the tumors.

Adult↗
PubMed1986

Regulation of Fructan Metabolism in Leaves of Barley (Hordeum vulgare L. cv Gerbel).

Excised primary leaf blades of barley (Hordeum vulgare L. cv Gerbel) rapidly synthesized large quantities of fructan in the light and, upon transfer to the dark, they rapidly degraded it again. In the course of such a light/dark cycle the activities of sucrose-sucrose-fructosyltransferase (SST), fructan hydrolase, and invertase were measured in cell-free extracts of the blades. SST activity increased 20-fold within 24 hours in the light and disappeared again upon transfer to the dark during a similar period of time. Cycloheximide inhibited the increase of SST activity in the light indicating de novo synthesis. The loss of SST activity in the dark, however, was unaffected by cycloheximide. No SST activity appeared in the light if photosynthesis was inhibited by lowering the CO(2) concentration in the atmosphere. However, SST activity and fructan synthesis were induced even in the dark and at a low CO(2) concentration when the leaf blades were immersed in a solution of sucrose. Several other sugars, maltose and fructose in particular, had the same effect. Trehalose induced SST activity but no fructan synthesis occurred. The activities of fructan hydrolase and invertase changed little during the light/dark cycle. It is suggested that the control of SST activity in conjunction with the supply of photosynthates plays a key role in the regulation of fructan metabolism.

Journal Article↗
PubMed1986

[Ultrasound diagnosis as a complement to sialography].

Over a period of one year, 82 patients with chronic inflammations, concrements, abscesses, cysts, Warthin's tumours, pleomorphic adenomas, lymphonodules and neoplasms of the salivary glands were examined by ultrasound and sialography. Morphological features are described, and the validity of diagnosis by ultrasound and sialography is discussed.

Abscess↗