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Biomedical subjects

W Sixl

Publications and source records attributed to W Sixl.

At least 19 recordsLinked to original sources
PubMed2006

Transmission of Campylobacter spp. in a poultry slaughterhouse and genetic characterisation of the isolates by pulsed-field gel electrophoresis.

1. Contamination of retail products with Campylobacter spp. during the slaughter of poultry is a well-known problem of product hygiene. Mechanical evisceration often leads to intestinal rupture and discharge of gut contents, which can contain zoonotic and human pathogens. Processes along the slaughter line cause aerosols and airborne droplets, containing bacterial loads. 2. To estimate the possible transmission routes of intestinal Campylobacter, 36 measurements of the bioaerosol (Andersen sampler and SKC BioSampler), 30 cloacal (of three flocks), 10 equipment and 4 sedimentation samples were tested for the presence of Campylobacter species. 3. The results imply that, in addition to contaminated equipment, which was Campylobacter-positive in 80% of cases, aerosols with peak values of 4.0 x 10(4) (test series 1) and 1.4 x 10(4) (test series 2) CFU/m3 also provide a potential vector for horizontal transmission. 4. To explore the genetic similarities of isolates from different origins, 18 isolates recovered from air, 26 cloacal, 8 equipment and 4 sedimentation isolates were analysed by pulsed-field gel electrophoresis (PFGE), using the restriction enzymes Sma I and Sal I. The similarity of cloacal isolates with isolates from equipment, air and sediment, suggest that the contamination is of intestinal origin. 5. There were direct links between Campylobacter-positive flocks and the presence of the same strains in the aerosol of the slaughter hall. Air as a potential source for microbial transmission must be taken into account.

Abattoirs↗
PubMed1999

Quality of water--quality of life.

Especially in developing countries, the problem of adequate drinking water supply is an ever growing one. Public health programmes have been established to improve the population's health conditions, but these programmes require big financial means for guaranteeing adequate supply of potable water and medical therapy for sick people. Too little emphasis is still put on regular testing of drinking water for microorganisms such as Aeromonas sp. and Vibrio sp. In a spot check analysis in various countries, the importance of Aeromonas sp. is shown--not a single sample complied with international norms and guidelines for drinking water.

Aeromonas↗
PubMed1998

Francisella tularensis in Dermacentor reticulatus ticks from the Czech Republic and Austria.

Host-seeking adult Dermacentor reticulatus ticks were examined for the prevalence of Francisella tularensis in an active natural focus of tularemia along the lower reaches of the Dyje (Thaya) river in South Moravia (Czech Republic) and adjacent Lower Austria, in four localities of the flood plain forest-meadow ecosystem during the spring of 1996. The ticks were pooled (10 male or female ticks per group) and inoculated subcutaneously in 4-week-old SPF mice. Dead mice were sectioned, the spleens were homogenized in PBS and passaged in mice, and impression smears from the spleens, liver and heart blood were stained by Giemsa. Twenty-five isolates of F. tularenis were recovered from 1098 pooled D. reticulatus: the minimum infection rate (MIR) is 2.3%. MIRs for 629 female and 469 male D. reticulatus were 2.4% and 2.1%, respectively. The prevalence varied according to locality, but did not significantly differ between the Moravian (2.2%) and Austrian (2.8%) sites. The monitoring of D. reticulatus for F. tularensis might be a valuable contribution to the surveillance of tularemia in Europe.

Animals↗
PubMed1997

Campylobacter spp. and Salmonella spp. in black-headed gulls (Larus ridibundus).

Cloacal examination of 41 juvenile black-headed gulls (Larus ridibundus) by cultivation demonstrated Campylobacter jejuni in 26 (63%) and Salmonella typhimurium in 21 (51%) of them. All the bird samples were collected in a breeding colony near the town Hodonin, South Moravia, Czech Republic in 1996. Twenty six Campylobacter isolates were tested for antibiotic and drug sensitivity: all were resistant to at least three agents (Penicillin, Tetracyclin and Sulfomethoxazol-trimethoprim) while all were sensitive to Augmentan, Cefotaxim, Ciprofloxacin, Erythromycin, Nitrofurantoin and Cephazidine. Four percent of isolates were resistant to Ampicillin and Nalidixic acid. Of the 21 S. typhimurium isolates tested, 33% were sensitive to all drugs assayed, proportions of the strains resistant to Sulfomethoxazol-trimethoprim, Tetracyclin and Streptomycin were 58%, 16% and 8%, respectively.

Animals↗
PubMed1997

Prevalence of Francisella tularensis in Dermacentor reticulatus ticks collected in adjacent areas of the Czech and Austrian Republics.

A total of 1098 host-seeking adult Dermacentor reticulatus ticks were examined in an active enzootic focus (floodplain meadow and forest ecosystem) of tularemia along the lower reaches of the Thaya (Dyje) river in South Moravia (Czech Republic) and adjacent Austria during the spring of 1996. Twenty-five isolates of Francisella tularensis were recovered (i.e., the overall minimum infection rate was 2.3%). The prevalence rate varied between 0.6% and 3.5% among four localities examined (two in Moravia and two in Austria) but the difference in the rate between Moravia (2.2%) and Austria (2.8%) has not been significant. The results show that D. reticulatus plays the role of an important vector in this extensive, perennial natural focus of tularemia.

Animals↗
PubMed1996

Serological examination of human and animal sera from six countries of three continents for the presence of rickettsial antibodies.

Forty samples each of human sera collected in Guinea Bissau, Cape Verde, El Salvador and Iran, and animal sera (goat and cattle from Sri Lanka and sheep from Tanzania) were examined for the presence of antibodies to typhus group (TG) rickettsiae, spotted fever group (SFG) rickettsiae and Coxiella burnetii by enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody (IFA) test. Of human sera tested, a higher proportion of positive sera were found with ELISA and IFA test for TG, SFG rickettsiae and C. burnetii in El Salvador (42.5 vs 20.0%, 40.0 vs 32.5%, and 27.5 vs 27.5%, respectively) and in Iran (25.0 vs 15.0%, 45.0 vs 27.5%, and 27.5 vs 25.0%, respectively), than in Guinea Bissau and Cape Verde, where they were less than 20.0% except for antibodies to SFG rickettsiae in Guinea Bissau (25.0% with ELISA and 20.0% with IFA test). While all animal sera were negative for the presence of antibodies to TG rickettsiae, a high proportion of sera from Sri Lanka reacted in ELISA and IFA test with SFG rickettsiae and C. burnetii (37.5 vs 20.0% and 27.5 vs 25.0% for goat sera, and 40.0 vs 30.0%, and 17.5 vs 15.0% for cattle sera, respectively). The results obtained indicate that the studied rickettsial diseases can be spread in given territories and may pose a public health problem requiring greater attention than has been paid so far. The suitability of ELISA and IFA test for serological survey of rickettsial antibodies is discussed.

Animals↗
PubMed1995

Salmonellae in gulls and other free-living birds in the Czech Republic.

Cloacal swabs, collected from 756 wild synanthropic and exoanthropic birds of 57 species in the Czech Republic, yielded 32 strains of Salmonella typhimurium [phage types (PT) 141, 104 and 41], six isolates of S. enteritidis (PT 8, 4 and 6e), and one each of S. panama and S. anatum. Except for one S. enteritidis isolate from a grey-lag goose (Anser anser) and one S. typhimurium isolate from a coot (Fulica atra), all of the other strains were derived from black-headed gulls (Larus ridibundus), of which 24.7% were found to be infected. The black-headed gull might play a role in the dispersal of pathogenic salmonellae.

Animals↗
PubMed1995

People on the garbage dumps of Cairo: a toxicological in vivo model?

In the outskirts of Cairo, some 40,000 people live on garbage dumps. These people form a closed population whose socio-economic problems are identical. The pollutants are evenly distributed. Up to 30% of the garbage on the polluted area, which cannot be recycled, is burned, resulting in a high concentration of pollutants in the environment. The concentrations of heavy metals, dioxins/furans, PCB, PAH in dust deposit and soil were measured as well as the air pollutants SO2, HCl and CO. It was shown that while the systemic immune system is only affected to a very small degree, secretory immunoglobulin A is strongly affected by the emissions. It could be demonstrated also an increased readiness for allergic respiratory disease, through the proof of hyperreactive mucous membranes. In the polluted area, 58% of the examined children were affected, whereas in the control area only 22% displayed a hyperreactive mucous membrane. Also the concentration of NANA (N-acetyl-N-neuraminic acid) in the serum, as a unspecific marker of cell irritation, was high in the serum of children from the polluted area. Most of the pollutants detected can also be observed in the industrialized regions, especially in combination with incinerating plants. In this way, this study suggests the synergistic effects of pollutants.

Air Pollutants↗
PubMed1992

[10 years foodborne listeriosis--an evaluation].

Foodborne listeriosis outbreaks, which occurred in the past 10 years, have raised new questions in listeriosis epidemiology. The renewed interest in listeriosis and Listeria monocytogenes has resulted in various efforts, which enormously enriched the knowledge about his relatively rare, but because of the high mortality rate, important infectious disease as well as about its causative agent. New data concerning epidemiology and therapy of human listeriosis as well as new experience with Listeria monocytogenes regarding virulence, typing, isolation, identification, occurrence in food and environment, behaviour towards disinfectants etc. have changed the view of human listeriosis prevention. The new aspects are summarized in this review.

Adult↗
PubMed1989

[Testing of industrial repellents against gnats and horseflies].

According to the Austrian Cosmetic Recommendation a minimum period of protection of 2 hours is to be provided. This applies to the preparation on the market with the substance "Merck 3535" in aqueous concentration in the tested area. However, this protection period was not achieved for horseflies. In test trials the characteristic of the individual nuisance should be taken into account.

Alanine↗
PubMed1989

[Air pollution emissions and changes in lung function].

Among the environmental hazards in a modern industrial society, air pollutants are of major significance. They can directly affect the cells of the bronchopulmonary system and lead to an irritation of the epithelial and immunocompetent cells. This results in a decreased secretion of immunoglobulin A, and, consequently, a decreased resistance against infections. Thereby pathogens, especially bacteria, may lead to an inflammation of the respiratory tract. Apart from the direct toxic effect of their pollutants, recurrent inflammations eventually lead to small airway obstruction. The spirometric parameters FVC, FEV1 and MEF50 were reduced. IgA concentration in the saliva was determined by means of immunodiffusion and was found to be reduced when compared to a less exposed group. A series of pathogenic germs were isolated from various mucous membranes (conjunctiva, nose/throat). The median immissions for the children examined were 1.345 mg/m3 SO2 and 0.189 mg/m3 HCl.

Air Pollutants↗
PubMed1989

Bacteriological investigations and resistance patterns in beef and goat's meat in Colombo's slaughter-house.

A high contamination rate was found in meat of slaughtered cattle and goats at the time of this study. Certain organisms were isolated from meat and also from wounds of slaughter workers. A high antibiotic resistance pattern was found - in some instances. Following observations of high surface meat contamination, associated with high antibiotic resistance, it must be assumed, that these highly resistant organisms can also be directly transmitted to humans by transport, transaction and by food preparation. This must be taken into consideration in the actual therapeutics.

Abattoirs↗
PubMed1989

On the drinking water situation on the Cape Verde Islands (Island of Santiago).

On the Cape Verde Islands drinking water investigations were made. The drinking water was tested in chemical and bacteriological respects as well as on amebas and rotaviruses. The isolated germs were investigated on their reaction against several antibiotics and chemotherapeutics by means of the agar diffusion test. The changing of drinking water quality from water-supply-places to the households was investigated as well. According to the fact, that only 10.6% of 207 public water supplies investigated show drinking water quality after ONORM, and that nearly 30% and 80.2% of them contained E. coli and coliforme germs respectively, the urgency of extensive sanitations of water supplies on the Cape Verde Islands is evident.

Amoeba↗