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Biomedical subjects

W Schilling

Publications and source records attributed to W Schilling.

At least 73 records · Page 4Linked to original sources
PubMed1983

Gluten-free diet in patients with dermatitis herpetiformis. Effect on the occurrence of antibodies to reticulin and gluten.

In a prospective study of 51 patients with dermatitis herpetiformis (DH), the occurrence of antibodies against reticulin and gluten was investigated and the influences of gluten-free, gluten-restricted, and normal diets on the persistence of these antibodies were compared. After a period extending from 11 to 47 months, none of the patients who were eating a gluten-free diet had reticulin or gluten antibodies. The patients eating a normal diet had the same incidence of reticulin autoantibodies at the end of the study as at its beginning, and gluten antibodies developed in some additional patients during the follow-up period. Thus, we have shown that the elimination of gluten from the diet of patients with DH has an important influence on the occurrence of antibodies to both reticulin and gluten.

Adult↗
PubMed1981

On the biochemical nature of the 'Sm' nucleoplasmic antigen.

The nucleoplasmic autoantigens RNP and Sm are of particular interest because of their associations with certain symptoms of mixed connective tissue disease and systemic lupus erythematosus. The RNP is generally thought to be a ribonucleoprotein and there is evidence that its RNA may be single-stranded. Experiments presented in this report are in support of the concept that the Sm-antigen may also be an RNA protein. Purified Sm-anti-Sm precipitates were shown to have high RNA contents and treatment of Sm-antigen with RNAase in a hypotonic medium strongly reduced in antigenicity. The latter effect indicates that the Sm-antigen may in contrast to the RNP contain double-stranded RNA, a possibility also suggested by the finding that the Sm-antigen was soluble in 2 M LiCl. The Sm-antigen was found further to differ from RNP in being selectively absorbed in BD-Sephadex, while RNP remained active in the supernatant. Cytochemical studies involving stimulation and inhibition experiments with lectins and RNA polymerase inhibitors showed that the Sm-antigen was, in distinction to RNP, sensitive to rifampicin but not to alpha-amanitine. This suggests that the RNAs of the nucleoplasmic antigens may be synthesized by different RNA polymerases.

Amanitins↗
PubMed1981

Some characteristics of immunofluorescence tests for antibodies against gluten, using wheat grain sections or gliadin coated sepharose beads.

Antibodies against gluten and gliadin were determined by the indirect immunofluorescence technique, using frozen wheat grain sections or gliadin-coated Sepharose beads. The methodological characteristics and diagnostic usefulness of the two techniques were evaluated. The reproducibility of both was improved by introducing a fluorometric reading-off procedure. Antibody quantitations were preferably performed as end-point titrations. The two techniques had different dose-response relationships. The grain section technique was more discriminative for small variations in antibody concentration than the bead technique. The latter was, however, more reproducible. Besides antibodies against gliadin, a number of patients with gluten enteropathy had antibodies against the main septa of wheat grains. Fluorescence intensity was preferably expressed in multiples of background intensity. A reaction was visually perceived when the fluorometrically-measured fluorescence intensity reached 2.5 times the background intensity. Using this value as the limit for positive reactions, antibodies were demonstrated in 81% of the cases with verified gluten intolerance, compared with 28% in cases with other intestinal allergies and 8% in normals. The diagnostic specificity of both the grain section and Sepharose bead technique for gluten enteropathy increased with increasing antibody concentration and was apparently 100% when the fluorescence intensity produced by a 1/10 serum dilution reached a value 7 to 8 times that of the background. Antibodies against reticulin were demonstrated in 1/4 of the cases having anti-gluten antibodies but in none of those with non-gluten-induced gastro-intestinal symptoms. Antibodies of the IgG class against cow's milk were demonstrated more often and in higher titre in cases with anti-gluten antibodies than in those without them.

Adolescent↗
PubMed1981

[Different patterns of latent pulmonary hypertension under physical exercise and their clinical significance].

The investigations pointed out that responses of the pulmonary hemodynamic in patients with COLD on physical exercises are highly different from those in normal subjects. The findings of pulmonary circulation on pink puffers (type A) and blue bloaters (type B) are differing in a specific manner. The lowest capacity of load is observed in patients of type A. Patients with clinical signs of type B show under exercise conditions the ability to approach their physical load capacity rather to that of normal persons. The features of prognosis, therapy and rehabilitation in the different patterns of pulmonary heart disease are discussed.

Cardiac Volume↗
PubMed1980

[The myocardfunction in chronic obstructive lung diseases with different pathogenesis (author's transl)].

The results of an investigation of the myocardial function are given. Described as contractility, ability of hypertrophy and reagibility in patients with chronic obstructive lung diseases under definate ergometric conditions. The patients are divided in three groups according to the proposition of Dornhorst and Nash: pink puffer or typ A, blue bloater or Typ B and mixing Typ M. Concerning contractility and hypertrophy differences between the groups are apparent significantly. The patients of typ B and M have the highest load by hypertrophy and contractility. The patients of typ A have only a small reserve of load. They are also not well adapted on exercise and have a high risk for cardiac insufficiency.

Adult↗
PubMed1980

[Thoracoscopic lung biopsy (author's transl)].

The authors start from the fact that till now was a declining attitude in face of the thoracoscopic lung biopsy. Under local anaesthesia any air embolism were happened with it. Moreover there was no optimal instrument for thoracoscopic lung biopsy. Beginning with pressure measurements of the intrathoracic cavity systems the authors research the conditions which can result in air embolism. They found that air embolism is excluded only by unilateral ventilation of the non-biopsied lung of the patient via a channel of Carlens' double-lumen catheter. The other channel of the cathetaer which is connected with the thoracoscopically biopsied lung must be aggreed with the atmosphere in the same way as the pleural space via the open thoracoscope tube. Further it was developed the suction punch, a special instrument for thoracoscopic lung biopsy. In this instrument the proceedings of catch and detache the tissue from the parenchyma are divided contrarily to the forcepslike devices. With this new instrument 63 patients were examined. The thoracoscopic lung biopsy was performed under general anaesthesia with side-separated ventilation through Carlens' catheter. The histologically verified diagnostic yielded was 89 per cent. There aren't serious complications.

Biopsy↗
PubMed1980

[The treatment of lung fibrosis (author's transl)].

D-Penicillamine is increasingly used for the treatment of lung fibrosis. The case-histories of 46 patients from 4 chest hospitals were analysed. All parameters being taken in consideration 13 patients showed an improvement, 17 patients no change and 16 patients a deterioration of their state of health. Side effects were observed in 22 patients.

Adult↗
PubMed1980

[Angiography, the efficacious means of the differential diagnosis of unspecific lung diseases, particularly as to hilum and mediastinum of the lungs (author's transl)].

Between sternum and spinal column, pathological changes of the mediastinum and of the adjacent areas are being not to be detected in the roentgenogram. Roentgenologically they are only to be detected in the event that there have been produced either protrusions or dilatations of the mediastinal shadow. The diagnosis of diseases of vessels, lymphnodes, tumors and inflammations, respectively, is very difficult to be obtained by roentgenogram of the chest in one or several planes. Beside endoscopic methods (bronchoscopy, thoracoscopy, mediastinoscopy), the angiography deserves to be emphasised among the various intrathoracal diagnoses. The angiography has found ever widening application with the reliable representation of processes of the heart and vessels in community with the defining of protruding processes. The angiography is to be used not only with suspicion of aneurysm and anomalies of the thoracal aorta, but with aneurysms of the heart wall, tumors of the heart, central arteriovenous fistulae of pulmonary vessels and with anomalies about the pulmonary artery, the pulmonary vein and the vena cava, respectively. Dependent upon the particular pathological changes stated, as methods of examination, the cavography and pulmonary angiography, respectively, are being applied. The statement has been supplemented by illustrating pictures.

Angiography↗
PubMed1979

[Chronic persistent hepatitis. Histological and clinical study of different forms of the course of CPH].

Among 4903 liver biopsies were found 96 cases (241 biopsies) of non-active hepatitis persisting for longer than one year (up to 12 years). Three forms of the course of chronic persistent hepatitis could be delimited in morphological terms taking into account clinical laparoscopic and clinical chemical data. The type Ia presents the picture of a largely subsided, lobular accentuated acute virus hepatitis. The type Ib corresponds to a chronic inflammation with emphasis on the portal system without destruction of the limiting plate, without fibrosis and with only slight intralobular involvement. The type Ic is characterized by a portal and slight septal fibrosis with round-cell infiltrates and a slight facultative periportally active inflammation as well as a moderate intralobular mesenchyme reaction. The typing permits a clear subdivision and a differentiated prognosis: 10% of the cases of type Ic pass into an active chronic hepatitis of the type IIa.

Adult↗
PubMed1979

Effect of pregnancy zone protein on leucocyte migration inhibition, lymphoycte transformation and rosette formation by lymphocytes.

Pregnancy zone protein from pregnancy serum and control preparations from male serum were tested in lymphocyte transformation tests, in a system for rosette formation by T and B lymphocytes and in leucocyte migration inhibition tests using PPD-induced inhibition. PZ-protein preparations caused a dose dependent inhibition of lymphocyte transformation after stimulation with PHA, PPD and MLC. Inhibition was most pronounced in the PHA-system. In the rosette tests there was a tendency for T lymphocytes to bind fewer SRBC after incubation with PZ-protein while no effect on B rosettes was observed. In contrast, PPD-induced inhibition of leucocyte migration was abrogated effectively by PZ-protein, but not by corresponding fractions from male serum.

B-Lymphocytes↗
PubMed1976

[Clinical ergometry in cardio-pulmonary functional disorders--a comparison of the usual ergometric technics].

It is reported on the results of a comparative examination of 18 patients with two usual ergometric methods (team pathophysiology of respiration and research group diseases of heart and circulation of the GDR). Referred to the intake of oxygen as a measure for the stress of the cardiorespiratory system by means of the ergometric methods recommended by the research group diseases of heart and circulation significantly higher minute volumes of the heart rate were established. Principal differences in the evidence of the two methods did not appear.

Adolescent↗
PubMed1976

Synergistic effect of alloantibodies or F(ab')2 and prednisone on murine split heart allograft survival.

Heterotopic grafting of split allogeneic mouse hearts across a strong H-2 barrier (A/Sn leads to A.CA) was used in a study of alloantibody or F (ab')2 induced enhancement. Strong specific binding of alloantibodies occurred in the graft. A marked synergistic effect on graft survival was observed after combined treatment of recipients with enhancing serum and prednisone. The highly dose-dependent enhancing effect of F (ab')2 was also strongly potentiated by supporting prednisone treatment. In vitro pre-incubation of the heart tissue with alloantiserum or F (ab')2 did not prolong graft survival; in contrast, C-mediated damage of the graft was suggested when serum was used.

Animals↗
PubMed1976

[Clinical examination of the conception '86 of "Canoer Multistep-Therapie" (CMT) by M. v. Ardene. First communication: the standard, the control-parameters, and the course of therapy (author's transl)].

It is reported about the clinical testing of the "Cancer Multistep Therapy" (CMT) by M. von Ardenne (CMT Concept '74). It was made in 1974/75 on 42 tumour-patients. At first we demonstrate in this publication clinical and paraclinical findings, which may be relevant for the therapy-standard, but the results of the therapy-efficiency will be given later. On the base of findings and observations we verify a program-true employment of the CMT Concept '74 and we demonstrate the pathophysiological reaction-patterns in connection with the longterm-hyperglycemia (glucose-utilization, spontaneous hyperthermy with tachycardia, spontaneous leukocytosis with typical deviations in the hematogram). These reaction-patterns are described particularly as "general adaptation syndrome" (Selye). Further we give an assessment of compatibility, tolerableness, and practicability of the CMT.

Antibody Formation↗