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Biomedical subjects

W Savage

Publications and source records attributed to W Savage.

At least 55 records · Page 3Linked to original sources
PubMed1982

Scanning electron microscopic study of lead effects on cerebral astrocytes in primary culture.

The effects of lead acetate on the cell surface of primary cultured astrocytes from dissociated neonatal rat cerebrums were studied using scanning electron microscopy. Astrocytes, grown for two weeks in a modified Eagle's minimal essential medium containing 20% calf serum, were exposed to lead (1, 10, 30, 50, or 100 micrograms Pb/ml) for four days. Astrocytes grown in lead-free medium showed membrane folds (ruffles) and microvilli on the surfaces of perikarya. Vesicular structures or blebs (gliosomes) were scattered on the surfaces of perikarya and branched cell processes. Cultures exposed to 1 microgram Pb/ml were indistinguishable from controls. After exposure to 10 and 30 micrograms Pb/ml, microvilli disappeared, membrane folds were less extensive, and the number of surface blebs increased. Astrocytes exposed to 50 micrograms Pb/ml showed no surface membrane folds or microvilli while the blebs increased sufficiently to cover the surface of the perikarya. Cells treated with the highest lead concentration (100 micrograms/ml) were shrunken and perikarya again were covered with blebs. Transmission electron microscopy of the cells exposed to 10-100 micrograms Pb/ml showed membrane bound vacuoles and areas of cytoplasmic clearing, some of which may correspond to the surface blebs seen by SEM. These studies suggest that the changes in the cell surface resulting from lead exposure represent a toxic effect of lead on primary cultured astrocytes.

Animals↗
PubMed1977

Asymptomatic gonorrhoea in males.

Over a 21 month period at Cook Hospital nine patients, or 10% of diagnosed cases of gonorrhoea in men were asymptomatic. The importance of this group in maintaining a reservoir of infection is noted. About half of these patients have been treated inadequately for gonorrhoea and the importance of post-treatment cultures and urine checks is stressed. Penicillin by injection is still the best method of treatment.

Adolescent↗
PubMed1976

Family planning in a hospital clinic.

The establishment of a hospital board family planning clinic and the results during its first year of operation are described. Three hundred and fifty-four patients were seen, a third of whom were interested in sterilisation. Only 3 percent presented with infertility and less than 1 percent referred themselves inappropriately. The failure rate in terms of unwanted unplanned pregnancies was 2 percent, and a further 1 percent had socially inconvenient but desired pregnancies. Of those established on contraception 45 percent chose the pill, 34 percent the intra-uterine device and approximately 10 percent each the injection or a barrier method;

Adult↗