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Biomedical subjects

W Reuter

Publications and source records attributed to W Reuter.

At least 37 records · Page 2Linked to original sources
PubMed1995

Multicentre evaluation of a non-wipe system for the rapid determination of total cholesterol in capillary blood, Accutrend Cholesterol on Accutrend GC.

Accutrend Cholesterol, a non-wipe test for the determination of total cholesterol in capillary blood, was evaluated at four clinical centres. Cholesterol determinations with the Accutrend system using capillary blood were compared with results obtained with the cholesterol oxidase/p-aminophenazone (CHOD-PAP) method using the respective capillary sera. Triacylglycerols, uric acid and haematocrit were determined to evaluate potential interference. Imprecision measurements were performed with venous blood. To examine the reproducibility of results from lot to lot, three different lots of test strips were included in these investigations. Results with Accutrend Cholesterol agree with those of the comparison method within systematic differences of +2.5% to -3.2%, depending on the lot. There was no interference by triacylglycerols up to 10.28 mmol/l (900 mg/dl), by uric acid 60 to 400 mumol/l (1 mg/dl to 7 mg/dl), or by haematocrits between 0.35 and 0.54. Impression data show coefficients of variation of generally less than 5%. Thus Accutrend Cholesterol proved to be a reliable system for the determination of total cholesterol.

Blood Chemical Analysis↗
PubMed1994

Utilization of light for nitrogen fixation by a new synechocystis strain is extended by its low photosynthetic efficiency.

Performance of photosynthesis and nitrogenase activity in a novel cyanobacterium, Synechocystis sp. strain BO 8402, isolated from Lake Constance, located at the northern fringe of the Alps in central Europe, and of a stable derivative, strain BO 9201, were examined. Strain BO 8402 is characterized by an extraordinarily high level of autofluorescence originating from paracrystalline phycobiliprotein-linker complexes located in inclusion bodies (W. Reuter, M. Westermann, S. Brass, A. Ernst, P. Böger, and W. Wehrmeyer, J. Bacteriol. 176:896-904, 1994). Energy transfer between paracrystalline phycobiliproteins and the photosystems is inefficient, resulting in a high oxygen compensation point and a decreased growth rate. The derivative strain BO 9201 exhibits hemidiscoidal phycobilisomes that support a high growth rate, even under low light intensities. Because of the differences in photosynthetic performance, anaerobic light-stimulated nitrogenase activity is maintained at higher light intensity in the original strain BO 8402 than in the derivative strain BO 9201. The results indicate that the formation of paracrystalline phycobiliproteins in Synechocystis sp. strain BO 8402 represents a hitherto-unknown means for a unicellular cyanobacterium to extend its capacity to fix nitrogen in the light.

Journal Article↗
PubMed1994

Structure, composition, and assembly of paracrystalline phycobiliproteins in Synechocystis sp. strain BO 8402 and of phycobilisomes in the derivative strain BO 9201.

The phycobiliproteins of the unicellular cyanobacterium Synechocystis sp. strain BO 8402 and its derivative strain BO 9201 are compared. The biliproteins of strain BO 8402 are organized in paracrystalline inclusion bodies showing an intense autofluorescence in vivo. These protein-pigment aggregates have been isolated. The highly purified complexes contain phycocyanin with traces of phycoerythrin, corresponding linker polypeptides LR35PC and LR33PE (the latter in a small amount), and a unique colored polypeptide with an M(r) of 55,000, designated L55. Allophycocyanin and the core linker polypeptides are absent. The substructure of the aggregates has been studied by electron microscopy. Repetitive subcomplexes of hexameric stacks of biliproteins form extraordinary long rods associated side by side in a highly condensed arrangement. Evidence that the linker polypeptides LR35PC and LR33PE stabilize the biliprotein hexamers is presented, while the location and function of the colored linker L55 remain uncertain. The derivative strain BO 9201 contains established hemidiscoidal phycobilisomes comprising phycoerythrin, phycocyanin, and allophycocyanin as well as the corresponding linker polypeptides. The core-membrane linker protein (LCM), and two polypeptides with M(r)s of 40,000 and 45,000 which are present in small amounts, exhibit strong cross-reactivity in Western blot (immunoblot) analysis using an antibody directed against the colored LCM of a Nostoc sp. In contrast, strain BO 8402 exhibits no polypeptide with a significant immunological cross-reactivity in Western blot analysis. Physiological and genetic implications of the unusual pigment compositions of both strains are discussed.

Bacterial Proteins↗
PubMed1994

[Specific parent training in alalia. 1: Introduction and presentation of the seminar concept].

Developmental aphasia is the most severe form of speech retardation and needs therapeutic intervention. The young age of these children (< 3 years) makes therapy difficult. A specified speech therapy is not possible at this age. Intensive stimulation of verbal communication by the parents is necessary. Therefore we developed a specialized training program for parents. This preparation is very useful for later therapy and should prevent disturbance of interaction between parents and child. We will present our concept.

Aphasia↗
PubMed1994

[Specific parent training in alalia. 2: Study of experiences of parents in the seminar].

Specified training for parents of children with developmental aphasia has been offered for several years. We want to guide the parents to a controlled stimulation of speech at home. We asked the former participants about their experiences with this training program. We also studied the realization of the program by parents in everyday life before their children started therapy. The experiences were mainly considered positive, realizing the program did not present difficulties.

Aphasia↗
PubMed1993

Core substructure of the hemiellipsoidal phycobilisome from the red alga Porphyridium cruentum.

The allophycocyanin core of hemiellipsoidal phycobilisomes from the red alga, Porphyridium cruentum, was isolated by chromatography on hydroxylapatite and subsequent density gradient centrifugation. Electron microscopy of negatively stained core complexes revealed a tricylindrical structure with a width of 21 to 23 nm in face view and a depth of 12 to 14 nm in side view. Fluorescence emission spectra of these complexes were similar to those of whole phycobilisomes confirming the presence of the two "terminal energy acceptors" allophycocyanin B (APB) and the high molecular linker polypeptide LCM. The polypeptide composition analyzed by SDS-PAGE showed the "anchor" polypeptide LCM, alpha AP, alpha APB, beta AP subunits, a low molecular weight linker with M(r) 13,500 and a blue-colored polypeptide of M(r) 19,800. A complex containing APB could be isolated from a "trimeric" allophycocyanin fraction of the density gradient by agarose gel electrophoresis in the presence of ampholytes. This complex shows a polypeptide composition of (alpha APB alpha AP2 beta AP3).L13.5C and contributes to the core with 30 to 35% of total "trimers". In comparison to the allophycocyanin cores from hemidiscoidal phycobilisomes of cyanobacteria and red algae, all data concerning the core of the hemiellipsoidal phycobilisomes from Porphyridium cruentum strongly suggest that there is no increase in size but an increase in its APB content. A model comprising the allophycocyanin core and the membrane integral photosystem II particles presents the structural and possible functional consequences of the results.

Bacterial Proteins↗
PubMed1992

[Population screening on a population level in Leipzig within the scope of the Bavarian cholesterol project].

Cholesterol determinations of 15.391 inhabitants of Leipzig were carried out in November 1990 within the Bavarian Cholesterol-Screening-Project. The aim of this screening on population basis was to identify individuals with elevated blood cholesterol levels as one of the risk factors of atherosclerosis. Capillary blood cholesterol was measured by means of the Reflotron dry-chemistry system (Boehringer, Mannheim). 27% of the inhabitants of Leipzig show cholesterol levels lower than 5.2 mmol/l as recommended by the European Society of Atherosclerosis Research. Furthermore the characteristic age dependence is seen. In comparison with the town Nürnberg lower mean cholesterol values were found in Leipzig.

Adult↗
PubMed1991

[The effect of oral trapidil therapy on clinical and hemorheological parameters in arteriosclerosis obliterans in comparison to pentoxifylline--a pilot study].

20 male patients from 44 to 61 years of age with arteriosclerotic disturbances of the blood supply of the legs (stage II after Fontaine) were in the cross-over treated with 3 x 200 mg trapidil or 3 x 200 mg pentoxifylline daily. The duration of the therapy was in each case 6 weeks with a one-week elutriation phase. Statistically ascertained was an increase of the distance of intermittent claudication for the two drugs in patients with initial values lower than 550 m. On the other hand the tibio-brachial Doppler quotient and the submaximal blood supply established by the venous-occlusion plethysmography did not change. The post-ischaemic recovery phase of the transcutaneous oxygen partial pressure at the lower leg shortened in particular under the influence of trapidil. Lipid parameter, haematocrit, fibrinogen and plasma viscosity remained unchanged. The subjective tolerance of the two remedies was good.

Adult↗
PubMed1990

Isolation of allophycocyanin B from Rhodella violacea results in a model of the core from hemidiscoidal phycobilisomes of rhodophyceae.

Two 'trimeric' allophycocyanin complexes could be isolated from the hemidiscoidal phycobilisomes of Rhodella violacea. AP = (alpha *AP alpha 2AP beta 2AP beta *AP) and APB = (alpha *AP alpha AP alpha APB beta 2AP beta *AP). Lc13.5APB. The isolation was performed by combined methods of gradient centrifugation, hydroxylapatite chromatography and 'native' polyacrylamide gel electrophoresis. AP showed the well-known spectral characteristics of allophycocyanin without linker polypeptide. APB is characterized by its long wavelength absorbing shoulder (675 nm) and fluorescence emission (682 nm), respectively. The existence of two low molecular linker polypeptides Lc12.5 and Lc13.5APB in the phycobilisomes of Rhodella violacea, their stoichiometric calculations and the localization of Lc13.5APB in allophycocyanin B facilitated the construction of a model of the phycobilisome core.

Electrophoresis, Polyacrylamide Gel↗
PubMed1990

[Oral trapidil treatment of Fontaine stage II arteriosclerotic circulatory disorders of the legs--a double-blind study].

In a double blind study altogether 41 patients with obliterating arteriosclerosis at stage II after Fontaine have been treated for 26 weeks either with 3 X 100 mg Trapidil (Rocornal) daily or placebo. In the second half of the study under Trapidil in contrast to placebo a clinically relevant additional increase of the distance of the intermitent claudication was the result. On the other hand, the plethysmographically measured reserve of the arterial blood supply did not show any changes in the two groups. A specific influence of Trapidil on the lipoprotein metabolism is apparently not present, since there appeared favourable influence on the hyperlipoproteinaemia diagnosed in 37 out of 41 patients both under placebo and under Trapidil.

Aged↗
PubMed1989

[Waist-hip and waist-thigh index in obesity especially in relation to weight reduction].

Waist-hip index (WHI) and waist-thigh index (WTI) were determined in 18 obese males and 24 females, respectively, before and after weight reduction, in comparison to this in 12 males and 10 females in normal weight. Under weight reduction the two indices in males were significantly reduced in correlation to the relative weight. In females analogous changes could not be proved. Furthermore, the WHI was significantly lower in persons with normal weight than in obese ones (males and females), the WTI only in females. - In obese males, however, not in males with normal weight the WHI processes a prediction value for the atherogenic risk, which is at least adequate to the value of up to now wellknown parameters (relative weight, body mass index etc.).

Adult↗
PubMed1989

[In vivo effects of high density lipoprotein rich serum fractions (HDLF) in the human. 1. Modification of circulating thrombocyte aggregates, cardiovascular parameters and lipid metabolism by HDLF].

By means of the modified Cohn-method 6 Cohn-IV-1-fractions were prepared in the small pool technique as sterile, pyrogen-free and HDL-rich serum fractions (HDLF), the HDL-concentrations of which were 15-20 g/l. In in-vitro investigations they inhibited the spontaneous aggregation of erythrocytes as well as the ADP-induced aggregation of thrombocytes and activated the molar and fractional initial cholesterol estirification rates as well as the prostacyclin synthesis. From HDLF intravenously applied in people as immediate effects result significant decreases of the cardiac pre- and afterload, an increase of the cardiac ejection fraction as well as a complete dissolution of circulating aggregates of thrombocytes. In 35 patients with hyperlipidaemias after infusion of 100 ml HDLF up to the 5th day increasing and for 14 days remaining increases of concentration of biliary bile acids, total phospholipids and of cholesterol were observed in lithogenic indices remaining in the normal region. The plasmatic lipo- and apolipoproteins reacted with increases, decreases or constancy of concentration which might be based on the different ethryopathogeneses of the hyperlipoproteinaemias.

Cholesterol, HDL↗
PubMed1989

[Modification of lipoprotein metabolism by antihypertensive therapy].

Of various antihypertensive drugs, particularly the unselective as well as the selective beta-receptor blockers but also diuretic drugs unfavourable effects on the lipid metabolism are reported, which above all consist of increases of triglycerides and decreases of HDL-cholesterol. The more modern antihypertensive drugs such as calcium antagonists, alpha 1-receptor blockers or angiotensin converting enzyme inhibitors according to the hitherto existing studies have no significant influence on the serum lipids. The final classification of the antihypertensive drugs regarding their influence on the atherogenic risk by negative changes in the lipoprotein metabolism is, however, at present not yet possible on account of insufficient long-term studies. For the reduction of adequate endangerings dietetic measures, reduction of overweight, physical training and when occasion arises change of the medication are recommended.

Antihypertensive Agents↗
PubMed1989

[Lipid metabolism and peripheral hemodynamics in arteriosclerosis obliterans treated with nifedipine--a comparison with pentoxifylline].

In the present study altogether 51 patients with obliterating arteriosclerosis stage II according to Fontaine were examined concerning the haemodynamic and lipid parameters under influence of nifedipine and pentoxifylline, respectively. An antiatherogenic effect under nifedipine takes place possibly by the diminution of the triglycerides in the serum and the blood pressure as well as by the increase of the linolenic acid in the serum (precursor of the vasoprotective prostacyclin I3). Pentoxifylline essentially behaves lipid-neutral. The increase of the intermittent claudication distance clearly speaks for pentoxifylline. - Nifedipine should only be used in concomitant indications.

Arteriosclerosis Obliterans↗
PubMed1989

[Clinical, biochemical and anthropometric studies and determination of biological age in relation to body mass in a longitudinal study].

On 46 adipose persons (25 males and 21 females) and 53 test persons with normal weight (35 males and 18 females) at the age of 25-40 years since 1976 clinical, biochemical and anthropometric investigations as well as the determination of the biological age have annually been performed. Several of the parameters established (e.g. cholesterol, triglycerides, fasting blood sugar, blood pressure among others) in the course of 9 years within the well-known normal regions draw towards the adequate valid limit value to the pathological entity. The results of the study distinctly show the central role of obesity for the development of risk factors. Despite the partly only with a certain tendency recognizable behaviour of single parameters after 9 years from our point of view the longitudinal method represents itself as a very well suitable method for establishing dynamic processes and risk factors of age.

Adult↗