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Biomedical subjects

W Qiu

Publications and source records attributed to W Qiu.

87 records · Page 5Linked to original sources
PubMed1995

Detection of brainstem auditory evoked potential by adaptive filtering.

A method of detecting brainstem auditory evoked potential (BAEP) using adaptive signal enhancement (ASE) is proposed and tested in humans and cats. The ASE in this system estimates the signal component of the primary input, which is correlated with the reference input to the adaptive filter. The reference input is carefully designed to make an optimal and rapid estimation of the signal corrupted with noise, such as ongoing EEG. With a good choice of reference input, it is possible to track the variability of BAEP efficiently and rapidly. Moreover, the number of repetitions required could be markedly reduced and the result of the system is superior to that of ensemble averaging (EA). To detect BAEP in cats, only 30 ensemble averages are needed to obtain a reasonable reference input to the adaptive filter, and, for humans, 350-750 ensemble averages are sufficient for a satisfactory result. Using the LMS adaptive algorithm, individual BAEP can be obtained in real-time.

Algorithms↗
PubMed1995

[A preliminary study on the mechanism of against human tongue cancer cell line].

Inverted phase contrast microscope and transmission electron microscope examination reveals that, tumor-draining lymphnode lymphocytes (DNL) conjugated with tumor cells very tightly at 12 hours co-culture. The DNL cell nucleus was seen positioned away from the target cells, whereas the cell mitochondria granules were oriented toward the point of contact with the target cells. The injured target cell had a highly condensed cytoplasm and chromatin and formation of cytoplasmic blebs, which were consistent with an apoptotic cell death rather than with a lytic or necrotic cell death. The mechanism was exocytosis of granules containing a pore-forming protein. Holes formed in the target cell's membrane may allow a lethal substance to enter and to induce its death via an apoptotic mechanism.

Apoptosis↗
PubMed1995

[Urinary calcium excretion in patients with pregnant hypertension syndrome].

We observed the urinary calcium in 103 patients in the third trimester of pregnancy and the relation between the 24 hour calcium excretion and the calcium/creatinine ratio of a single void urine sample. The study population included 75 normal pregnant women and 28 pregnant woman with pregnant hypertension syndrome. The 24 hour urinary calcium excretion in the patients with pregnant hypertension syndrome was significantly lower (0.70 +/- 0.36 mmol/L) than that in normal pregnant women (4.00 +/- 1.90 mml/L) P < 0.0005. The 24 hour calcium excretion correlated well with the calcium to creatinine ratio of a single void morning urine sample in the two groups. We conclude that the urinary calcium excretion can be used as an indicator for pregnant hypertension syndrome. The 24 hour urinary calcium excretion can be estimated from a single void urine sample. Pregnant hypertension syndrome is associated with hypocalciuria, probably due to increased tubular reabsorption of calcium.

Adult↗
PubMed1994

[Etiology of blindness in remote area: a clinic report].

This paper analyzes the cause for blindness in 408 patients of Xinfeng People's Hospital from 1977 to 1991. The results show that cataract is the major cause for blindness (32.84%). The others are eye injury in patients below age 46, cataract and glaucoma in patients above age 46. It is suggested that the stress of prevention and treatment for blindness should be put on the prevention of eye injury in juveniles and middle aged people, and the treatment for senile cataract in remote mountain areas.

Adolescent↗
PubMed1994

Visual evoked potential measurement by adaptive filtering.

The method of ensemble averaging (EA), commonly used to improve signal-to-noise ratio (SNR) in evoked potential measurements, loses potentially useful information regarding response variability across trials. Due to the usually low SNR, it is difficult to extract the response from individual trials. The present paper describes an adaptive signal enhancer (ASE) that is capable of tracking the variation of visual evoked potential (VEP) from trial to trial as well as improving SNR. This ASE requires two inputs: a primary input, which is the raw noise-corrupted response, and a reference input, which is designed for an optional performance of ASE. We used moving ensemble averaging (MEA) to form the dynamic reference input. Experimental results in humans show a satisfactory performance of the ASE in determining VEP to single stimulus and in tracking the temporal changes of the signal.

Algorithms↗
PubMed1994

An enhanced approach to adaptive processing of the brain stem auditory evoked potential.

The present paper describes an adaptive signal enhancer (ASE) method for improving signal-to-noise ratio (SNR) and also for tracking the variation of brain-stem auditory evoked potential (BAEP). The enhancer has two inputs: a primary input which is the original data to be processed, consisting of signal badly corrupted by noise, and a reference input. A method called dynamic averaging (DA) is introduced to obtain the reference input. The improved reference signal allows closer tracking of evoked potential in each subsequent trial. For BAEP measurement in human, the SNR is typically very low. It requires about 200 ensembles to generate the reference input acceptable for ASE, and tracking of the variation of BAEP can be obtained satisfactorily. Our results show that while ensemble averaging is still needed, the total number of ensembles is greatly reduced. The adaptive method thus reduces the time of measurement for BAEP compared with the conventional method of ensemble averaging.

Electricity↗
PubMed1993

[Immunological diagnosis of light chain disease--analysis of 11 cases found in Fujian Province].

This paper reports 11 cases of light chain disease (LCD) sequentially found in several coastal cities of Fujian Province. Immunological classification of this group of LCD was as follows: 6 belonged to lambda type, 4 were kappa type, and 1 was double LCD. We found that LCD was common in Fujian next only to MM of IgG class and accounted for 20% of the total 55 MM cases found in recent years. Several important points should be stressed when making immunological diagnosis of LCD. 1) It is well known that in most patients with LCD, M protein or Bence Jones proteinemia (BJPemia) is not detectable by conventional electrophoresis. Our studies show that running serum protein along with urinary BJP on the same one gel plate can allow for the recognition of small and faint band (s) of free light chain in serum, and also provides a rapid and sensitive method (immunofixation) to directly detect urinary light chains. 2) The concurrent presence of albumin with BJP in urine may interfere with the routine BJP test and lead to misdiagnosis. 3) As a result of the molecular seiving effect, the presence of light chain polymers in high BJP urine may lead to the presence of several parallel bands on the BJP gel electrophoretic spectrum, 4) Determination of light chain in urine is essential for final immunological diagnosis of LCD, but care should be taken, since many patients with other immunological types of MM may have a positive BJP test.

Bence Jones Protein↗
PubMedDate not supplied

Metabolites and analogs of 1alpha,25-dihydroxyvitamin D(3): evaluation of actions in bone.

Analogs of 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)] activate both genomic mechanisms via the nuclear vitamin D(3) receptor (nVDR) and nongenomic pathways via the plasma membrane vitamin D(3) receptor (pmVDR). Both of these pathways are normally activated by 1alpha,25(OH)(2)D(3), but as a result of synthesis of numerous analogs of 1alpha,25(OH)(2)D(3) these pathways can be distinguished. We used increasing doses of vitamin D(3) analogs to determine their potencies of action on these two distinct pathways, measuring calcium channel potentiation as an indicator of the nongenomic action and measuring increases in osteocalcin mRNA and protein release and bone resorption as indicators of genomic action. We found that both 25(OH)-16,23E-diene-D(3) (R) and 1alpha,25(OH)(2)-16,23E-diene-D(3) (A) are 10-fold more potent than 1alpha,25(OH)(2)D(3) for activation of the nongenomic pathway because double bonds in the side chain and the D ring increase the affinity for calcium channel potentiation. While the C-1alpha-hydroxyl group is not necessary for potentiation of calcium channels, methyl groups at this position can alter the affinity for calcium channel potentiation. On the other hand, 1000 fold higher concentrations of nongenomic analogs were needed compared to 1alpha,25(OH)(2)D(3) to increase osteocalcin mRNA or protein release. 1alpha,25-Dihydroxy-16-ene-23-yne-26,27-hexafluorovitamin D(3), (E) is an agent that is 10 fold more potent than 1alpha,25(OH)(2)D(3) at increasing osteocalcin mRNA and protein release, whereas 1alpha,25(OH)(2)-3-epi-D(3) increases osteocalcin mRNA and protein with a potency over 10 fold lower than 1alpha,25(OH)(2)D(3). These results suggest that double bonds in the side chain and the D ring stabilize action on the nongenomic pathway whereas F(6) on the terminal portion of the side chain increases potency for nVDR. On the other hand, while the C-1alpha-hydroxyl group is necessary for activation of genomic events via nVDR, the activation of nongenomic events occurs in the absence of this group.

Animals↗
PubMedDate not supplied

Use of green fluorescent protein-conjugated beta-actin as a novel molecular marker for in vitro tumor cell chemotaxis assay.

To study the dynamics of actin cytoskeleton rearrangement in living cells, an eukaryotic expression vector expressing a beta-actin-GFP fusion protein was generated. The expression construct when transfected into NIH3T3 fibroblast, A2058 human melanoma and 293T human embryonic kidney carcinoma cell lines expressed beta-actin-GFP fusion protein, which colocalized with endogenous cellular actin as determined by histoimmunofluorescence staining. The beta-actin-GFP was also observed to be reorganized in response to treatments with the chemoattractant type IV collagen. Cells extended pseudopodial protrusions and altered the morphology of their cortical structure in response to type IV collagen stimulation. More importantly, beta-actin-GFP accumulated in areas undergoing these dynamic cytoskeleton changes, indicating that beta-actin-GFP could participate in actin polymerization. Although ectopic expression of beta-actin-GFP lead to minor side effects on cell proliferation, these studies suggest that this strategy provides an alternative to the invasive techniques currently used to study actin dynamics and permits real-time visualization of actin rearrangements in response to environmental cues.

3T3 Cells↗
PubMedDate not supplied

Outgrowth of chondrocytes from human articular cartilage explants and expression of alpha-smooth muscle actin.

The objectives of this study were to investigate the effect of various enzymatic treatments on the outgrowth of chondrocytes from explants of adult human articular cartilage and the expression of a specific contractile protein isoform, alpha-smooth muscle actin, known to facilitate wound closure in other connective tissues. Explants of articular cartilage were prepared from specimens obtained from patients undergoing total joint arthroplasty. The time to cell outgrowth in vitro was determined and the expression of alpha-smooth muscle actin shown by immunohistochemistry. Treatment of the explants with collagenase for 15 minutes reduced the time to outgrowth from more than 30 days to 3 days. Hyaluronidase, chondroitinase ABC, and trypsin applied for the 15-minute period had no effect on the time to cell outgrowth when compared with untreated controls. Pretreatment with hyaluronidase prior to collagenase reduced the time to outgrowth. A notable finding of this study was that the majority of chondrocytes in the adult human articular cartilage specimens and virtually all of the outgrowing cells contained alpha-smooth muscle actin. We conclude that human articular chondrocytes have the capability to migrate through enzymatically degraded matrix and express a contractile actin isoform. Collagenase treatment reduces the time required for cell outgrowth.

Actins↗