Biomedical subjects

W Qiu

Publications and source records attributed to W Qiu.

At least 73 records · Page 4Linked to original sources

PubMed1998

[Arthroscopic sclerotherapy and suturing for disc displacement of the TMJ].

OBJECTIVE: The generation of the scar using TMJ arthroscopic sclerotheray in retrodiscal tissue needs some weeks. As the sclerotherapy can't fix disc reposited at once, we designed some new methods of discal traction suture with posterior sclerotherapy and anterior release. METHODS: 30 cases with 15 joints of disc displacement with reduction and 16 joints of disc displacement without reduction were treated with the above-mentioned techniques. RESULTS: The mean follow-up period was 27 mon (range 6-39). According to the rang of interincisal distances during maximum active opening and painful level using a standard 100 mm visual analog scale, and, analyzing an eight-item jaw pain questionnaire and jaw function using 5 item jaw function questionnaire, the total success rate was 74.2% (23/31), the success rate of disc displacement with reduction was 93.3% (14/15), and the success rate of disc displacement without reduction was 56.4% (9/16). CONCLUSION: TMJ arthroscopic disc traction suture with posterior sclerotherapy and anterior release is effective on disc displacement. Diagnosis, treatment and post-operative evaluation can be performed in the same arthroscopic procedure.

Adult↗

PubMed1998

[Phacoemulsification and foldable intraocular lens implantation through a clear corneal incision].

OBJECTIVE: To evaluate the therapeutic effects of phacoemulsification with foldable intraocular lens implantation through a clear corneal incision. METHODS: The surgery was performed through a 3.2 mm clear corneal incision on 100 eyes of 90 patients with senile, complicated, congenital and traumatic cataracts. RESULTS: Postoperatively, the visual acuities with spherical correction or without correction were 0.5 or better in 93.0% of eyes at one day, and 1.0 or better in 56.0%, 65.1% and 67.5% at one week, one month and three months respectively. The mean postoperative astigmatism was 0.93 +/- 0.58 D at one week and 0.20 D more than the mean preoperative one (P < 0.001). There was no statistical difference between the mean preoperative astigmatism and postoperative astigmatism at one and three months which were respectively 0.78 +/- 0.60 D and 0.73 +/- 0.59 D. CONCLUSION: The phacoemulsification with foldable intraocular lens implantation through a clear corneal incision can reduce early postoperative astigmatism, shorten the period of visual rehabilitation and simplify operative procedures.

Adolescent↗

PubMed1998

[Experimental study of radiation effects on the mandibular microvasculature of the guinea-pigs].

In the experiment, both 180 kV X-ray and cobalt-60 radiation were delivered to right mandibles of 128 guinea-pigs with different dose (1000 cGy x 4, 800 cGy x 4, 600 cGy x 4, 400 cGy x 4 within 1 week). Dental extractions were conducted 1, 3, 5 months after radiation and the irradiated animals were examined by using microvascular corrosion castings of mandibles. It was found that the number of capillaries reduced and microvascular networks broke off after radiation with small veins shrinking subsequently while the alterations of arteries were slight. This result indicated that capillary was the most vulnerable part to the damage of radiation in the vascular system. In addition the defects of vascular networks around the non-healing tooth extraction sites were also discovered with irregularly capillary proliferation in the periphery of the lesion. The phenomena suggested that microcirculation failure induced by trauma-repairing process lead to occurRence of osteoradionecrosis of jaws.

Animals↗

PubMed1998

[Low frequency of precore stop mutation in patients with dual infection of hepatitis B and C viruses].

In order to study the mutation of hepatitis B virus(HBV) precore gene in patients with dual infections of HBV and HCV and its possible clinical significance, the precore stop codon mutation (stop 28) was analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphism(RFLP) in patients with chronic liver disease, which were 25 cases with HBV DNA and HCV RNA positive(group A) and 31 cases with HBsAg and HBV DNA positive but anti-HCV and HCV RNA negative (group B). The results showed that the serum HBV DNA positive rate by 1st PCR in patients with dual infections(group A, 16%) was significantly lower than that in patients with HBV infection alone (group B, 65%), the stop 28 positive rate was also significantly lower (28%, versus 68%). The results suggest that the low frequency of precore stop codon mutant in patients with HBV and HCV dual infections was possibly due to low replication of HBV.

Adolescent↗

PubMed1997

cGMP stimulates sodium and chloride currents in rat tracheal airway epithelia.

To test the hypothesis that guanosine 3',5'-cyclic monophosphate (cGMP) regulates ion transport in airway epithelial cells, we measured short-circuit current (I(sc)) and (22)Na+ fluxes in primary cultured rat tracheal epithelial cells. In Cl- -containing Ringer solution, I(sc) was increased by approximately 17 microA/cm2 after application of 1 mM 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP), whereas, in Cl- -free solutions, the Na+ -mediated component was approximately 5 microA/cm2, suggesting a cGMP stimulation of Cl-secretory current and a smaller Na+ absorptive current. Inward and net mucosal-to-serosal (22)Na+ flux was doubled in the presence of 2 mM 8-BrcGMP. To determine whether nucleotide-gated channels play a role in this transepithelial Na+ absorption, blockers of nucleotide-gated cation channels were used to inhibit I(sc). The cGMP-stimulated Na+-mediated I(sc) was blocked by as little as 500 nM dichlorobenzamil or 50 microM L-cis-diltiazem, which are known blockers for cyclic nucleotide-gated cation channels. These agents also blocked the basal (non-cGMP-stimulated) current when measured in the presence of 10 microM amiloride, which blocks current through 5-pS amiloride-sensitive Na+ channels. To document whether the distribution of nucleotide-gated nonselective cation channels was consistent with a role in airway epithelial transport, in situ hybridization was performed. In situ hybridization of mRNA encoding for nucleotide-gated cation channels was found in epithelial cell layers of rat trachea, bronchi, bronchioles, and alveolar cells but not in smooth muscle layers or tracheal cartilage. Reverse transcriptase-polymerase chain reaction, restriction enzyme analysis, and sequencing of the cDNA transcribed from mRNA of whole lung and tracheal epithelial cells indicate that a channel highly homologous to the retinal nucleotide-gated nonselective cation channel (CNG1) is present. Thus these data, along with evidence supporting the existence of signal transduction pathways elevating intracellular levels of cGMP, indicate that cGMP regulates transepithelial ion transport in lung epithelial tissues.

Amiloride↗

PubMed1997

Cloning and widespread distribution of the rat rod-type cyclic nucleotide-gated cation channel.

We used Northern blot analysis, ribonuclease protection assay (RPA), reverse transcriptase-polymerase chain reaction, and in situ hybridization to investigate the hypothesis that the CNG1 isoform of the cyclic nucleotide-gated nonselective cation channel may be widely distributed in tissues of the rat. A cDNA encoding the CNG1 isoform was isolated from rat eye and human retina, and partial sequences were isolated from rat pineal gland and human kidney. Northern blot analysis revealed a 3.1-kilobase (kb) CNG1 transcript in rat eye, pineal gland, pituitary, adrenal gland, and spleen, and a larger transcript of 3.5 kb was found in testis. RPA confirmed the identity of CNG1 mRNA in rat eye, lung, spleen, and brain. Polymerase chain reaction-based detection of the mRNA for CNG1 indicates that the channel is expressed in lower abundance in many other tissues, including thymus, skeletal muscle, heart, and parathyroid gland. The cellular distribution of CNG1 was further studied by in situ hybridization, which demonstrated expression of mRNA in lung, thymus, pineal gland, hippocampus, cerebellum, and cerebral cortex but not in heart or kidney.

Amino Acid Sequence↗

PubMed1997

[Transposition of the fibular skin flap of the great toe to repair the fore plantar defect].

The sole, as the body weight-bearing region, is characterized with the skin of thick stratum corneum. Wound in the region is difficult to cure. Based on the blood supply of the foot, a fibular skin flap of the great toe was designed and used to treat the defect of the planta pedis. Clinical applications in 6 cases were successful. The operation results were satisfactory.

Adult↗

PubMed1997

[nm23 expression and its correlation with lung metastasis in human salivary adenoid cystic carcinoma].

The nm23 gene products, nucleoside diphosphate kinase (NDPK), expression in salivary adenoid cystic carcinoma (ACC) was evaluted by using LSAB immunohistochemical method. Of 25 cases tested, 16 (64.0%) showed positive staining, in which, higher incidence of positive staining was found in ACC without lung metastasis (88.2%, 15/17) than in that with lung metastasis (12.5%, 1/8; P < 0.01). Expression of NDPK/nm23 was correlated with the P-TNM pattern (P < 0.05), otherwise it was not correlated with pathologic types (P > 0.05). The result suggest that the nm23/NDPK may play a role in suppressing the metastatic potential in ACC.

Carcinoma, Adenoid Cystic↗

PubMed1997

[The different influences of splenectomy plus ligation of pericardial vein and shunt on portal hypertensive gastropathy].

A prospective and controlled study has been made to probe into the different influences of splenectomy plus ligation of pericardial vein (SPLPY) and shunt on portal hypertensive gastropathy (PHG). 26 patients with cirrhosis of liver and portal hypertension admitted from January 1994 to April 1996 were randomized into two groups. One group was treated with SPLPV (15 cases), and the other shunt (11 cases). Gastroscopy was made in all patients pre- and postoperatively to observe the changes of gastric mucosa. It has been shown that SPLPV exacerbate PHG (P < 0.05) and the shunt has an opposite effect (P < 0.05). We consider that it is necessary to modify SPLPV and reevaluate the clinical effects of the both.

Adult↗

PubMed1997

[The primarily study on the correlation between the cephalometric characteristics and the results of ploysomnography].

A study on correlation between temporomandibular joint ankylosis and Obstructive Sleep Apnea Syndrome (OSAS) was conducted. The statistic results demonstrate that there is significant correlation between (TA + SPA)/OPA and AI, and there is negative correlation between PAS and AI, (TA + SPA)/OPA and SaO2min by correlation and step regression between the cephalometric analysis and polysomnogram of fourteen cases of OSAS patients.

Adolescent↗

PubMed1997

[B-ultrasonographic diagnosis of emergency complications in lung cancer patients].

OBJECTIVES: To assess the value of B-ultrasonography in the diagnosis and treatment of emergency complications in lung cancer patients. METHODS: Thirty-five patients with lung cancer who suffered from emergency conditions were examined with B-ultrasonography. Of the 35 cases, 5 had superior vena caval syndrome due to compression by a centrally located tumor mass in the right upper lobe; 10 had moderate to large accumulation of fluid in the pericardium with cardiac tamponade; 2 had metastasis to the myocardium and pericardium with acute hemorrhagic pericardial effusion; 20 had massive pleural effusion. The ultrasonographic features of these complications were described. Patients with pericardial or pleural effusion received intervention treatment with Elemen emulsion under B-ultrasound guidance. RESULTS: Combined with systemic chemotherapy, in 4-week period, complications were temporarily ameliorated in 82.8% and became clinically stable in 8.5% of the cases. Treatment was of no effect in 8.5%. CONCLUSION: B-ultrasonography is of value in the diagnosis of emergency complications of lung cancer and is of help to guide intervention treatment.

Adult↗

PubMed1997

[Experimental study on antitumor effect in vitro of modified DNL of oral squamous cell carcinoma with TNF-alpha gene].

The gene coding for TNF-alpha was introduced into DNL of human oral squamous carcinoma by retroviral-mediated gene transduction, and experiments proved that the gene had been introduced into the DNL and the DNL could secrete TNF-alpha of high activity with PCR analysis, bioactivity analysis of TNF-alpha, selection test in G418. Cytotoxicity of gene-transduced and nontransduced DNL to Tca8113 cell was compared with MTT method. The result showed that cytotoxicity of the former was higher than that of the latter.

Carcinoma, Squamous Cell↗

PubMed1996

Factors related to the donor organ are major determinants of renal allograft function and survival.

In this study, we analyzed the relative impact of donor and recipient variables on cadaveric renal allograft function and survival. The unique feature of the study population is that each pair of recipients received their allografts from a single donor. The study includes 378 adult patients. In 129 pairs both recipients were Caucasian, and in 60 pairs one recipient was Caucasian and the other was African-American. All transplants were done in one center, thus minimizing differences in preservation time and providing uniform posttransplant management. The initial analysis showed a relationship between the function of the allograft 6 months after transplantation (serum creatinine [SCr]6 mo) and donor variables (P = 0.0004, analysis of variance). Furthermore, it was calculated that 64% of the variability in the SCr 6mo among patients was due to donor factors and 36% was due to recipient factors. An elevated SCr 6 mo was significantly associated with older donors, male recipients, and patients with acute rejection episodes. Furthermore, other unidentified donor factors may have an impact on allograft function. Reflecting the importance of donor factors, there was a significant relationship between SCr 6mo in paired recipients (P < 0.0008 by Spearman). Analysis of racially dissimilar pairs showed that the SCr 6mo and graft survival 6 months after transplantation were not significantly different between Caucasians and African-Americans. However, beyond 6 months, graft survival was worse in African-Americans (P < 0.0001 by Cox). Compared with Caucasians, graft survival was significantly worse in African-Americans with poorly controlled blood pressure (mean arterial pressure > 105 mmHg) (P = 0.002, Cox), but not in those patients with mean arterial pressure < 105 mmHg. In conclusion, donor factors are major determinants of renal allograft function. However, those factors may not be easily identifiable or quantifiable. Donor factors do not contribute to racial differences in allograft survival. However, poorly controlled hypertension correlates with poor renal graft survival in African-Americans.

Adult↗

PubMed1996

Studies of Choristoneura fumiferana nuclear polyhedrosis virus gene expression in insect cells.

To investigate the mechanisms regulating baculovirus virulence and host range we have begun to study Choristoneura fumiferana nuclear polyhedrosis virus (CfMNPV) and its gene expression in permissive and nonpermissive cells. We have identified and mapped three genes on the CfMNPV genome. The polyhedrin gene is located from 0.0 to 2.0 m.u. and two other genes, dnapol and p143, both of which are essential for baculovirus DNA replication, are located from 35.3 to 40.9 m.u. and 55.5 to 63.4 m.u., respectively. To gain insight into the expression of CfMNPV genes in permissive C. fumiferana and nonpermissive Spodoptera frugiperda cells, we constructed three expression plasmids in which the promoter region of the dnapol, the p143, and polyhedrin genes were placed in front of a chloramphenicol acetyltransferase reporter gene. All three CfMNPV promoters were active in nonpermissive cells in the presence of Autographa californica nuclear polyhedrosis virus (AcMNPV) DNA, but no activity was detected in permissive cells either in the presence of CfMNPV DNA or AcMNPV DNA. This lack of promoter activity was not due to failure of viral or plasmid DNA to enter the cell nucleus. It was possible that the reporter plasmids were inefficient templates for transcriptional transactivation so we developed a CfMNPV transfer vector and generated a recombinant virus in which the polyhedrin promoter driving CAT gene cassette was integrated into the CfMNPV genome. In this case, the CfMNPV polyhedrin promoter was highly active in the permissive cells.

Animals↗

PubMed1996

[The selection of highly lung metastatic salivary adenoid cystic carcinoma clone].

After 5 times repeated selection in vivo, combined with cloning technique in vitro and analysing of platelet aggregation activity, we had selected a highly lung metastatic salivary adenoid cystic carcinoma clone M-5clone24 (Acc-M). Compared with Acc-2, its metastatic rate was 96% vs. 18%; the weight of metastatic lung was 0.88 g vs. 0.31 g. The metastatic rate and the weight of metastatic lung positively correlated with platelet aggregation activity. The aggregation activity might be used as a useful parameter to assess Acc metastasis potential.

Animals↗

PubMed1996

[The MRI of cervical lymph node metastasis with malignant tumor of the head and neck].

A prospective diagnostic study on cervical lymph node metastasis from malignant tumor of the head and neck using MRI was performed for 62 cases in order to assess the potential or limitation of MRI for demontrating the pathological cervical lymph node. The double blind method was used in the study. The result reveals MRI has obvious higher sensitivity (85.7%), higher specificity (94.8%) and higher accuracy (89.6%) than palpation. Besides changes in size and shape of the lymph nodes, the intensity of MRI inside lymph nodes was altered showing, mixed hypo-iso-intensity in T1WI and hyper intensity in T2WI. Thus, MRI will probably play an important role as a staging modality in the detection of malignant lymph nodes metastasis.

Adult↗

PubMed1995

Impact of serum lipids on long-term graft and patient survival after renal transplantation.

The results after primary cadaveric renal transplantation in 665 consecutive patients were reviewed with respect to posttransplant serum lipids. Data were available for 182 of 665 patients on serum total cholesterol and triglycerides at 1 year posttransplant. Hypercholesterolemia (cholesterol > 200 mg/dl) developed in 141 of 182 patients (77%) and hypertriglyceridemia developed in 73 of 166 patients (44%). At 1 year posttransplant, hypercholesterolemia and hypertriglyceridemia both correlated with age at transplant (P = 0.0001, P = 0.01). Hypercholesterolemia and hypertriglyceridemia were also correlated with obesity as determined by body mass index (kg/m2) (P = 0.006, P = 0.01). Hypertriglyceridemia at 1 year posttransplant correlated with pretransplant triglyceride level (P = 0.006), but hypercholesterolemia did not correlate with pretransplant cholesterol level (P = 0.53). Hyperlipidemia was not correlated with cyclosporine (CsA) or prednisone dose (mg/kg), CsA trough levels, number of rejection episodes, or serum creatinine at 1 year. Despite significant differences in serum cholesterol and triglycerides, actuarial graft and patient survival were similar between the normolipidemic and hyperlipidemic groups.

Adult↗

PubMed1995

Obesity as a risk factor after combined pancreas/kidney transplantation.

The impact of pretransplant overweight/obesity was analyzed in a group of 268 consecutive primary pancreas renal transplant recipients. Obesity was defined as body mass index (BMI) greater than 27 kg/m2. BMI was available for 240 of the 268 patients. A total of 88% (212/240) of the patients had a BMI < or = 27 and 28/240 (12%) had BMI > 27. There were no significant differences in age, sex, or race between obese and nonobese patients. The incidence and degree of posttransplant hypertension, weight gain, increase in BMI, and hyperlipidemia did not differ on the basis of pretransplant BMI. Serum creatinine at one year posttransplant was slightly increased in obese patients, but the increase was not statistically significant. Cumulative prednisone dose (mg/kg) as well as cyclosporine (CsA) dose (mg/kg) at one year was not significantly different between obese and nonobese patients. However, there was a marginally significant negative correlation between BMI and one-year cumulative (mg/kg) prednisone dose (P = .06). Types and frequency of posttransplant complications were similar between obese and nonobese patients, although there was a slightly higher incidence of wound related complications in obese patients (11% vs. 6.8%) (P = NS). There was no difference in the frequency of acute rejection episodes in obese and nonobese patients. Actuarial patient survival was comparable between patients with BMI < or = 27 versus those with BMI > 27 (P = .10). However, actuarial graft survival, both pancreas and renal, were significantly decreased in patients with BMI > 27 (P = .029). The decrease in pancreas and kidney graft survival in obese patients could not be attributed to decreased "early" patient survival, increased incidence of perioperative or postoperative complications, differences in hypertension, acute rejection episodes, serum lipids, or immunosuppression dosage. The most common causes of graft loss were rejection and patient death in both obese and nonobese patients. After three years posttransplant, the decreased pancreas and renal graft survival in obese patients corresponded to decreased patient survival. The most common cause of patient death was cardiovascular complications in both obese and nonobese PKT recipients.

Adult↗