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Biomedical subjects

W Mohr

Publications and source records attributed to W Mohr.

At least 163 records · Page 9Linked to original sources
PubMed1978

[Proliferation of synovial fluid cells (author's transl)].

Investigations with labelled thymidine indicated that the mononuclear cells of the synovial fluid only rarely proliferateunder physiological conditions. Even in cases of rheumatoid arthritis no elevated proliferation rate was found. Cytophotometric investigations on Feulgen-stained nuclei is another way to determine cellular proliferation. To answer the question if the relative content of neutrophilic granulocytes in synovial fluids influences the proliferation of mononuclear cells, the DNA content of these cells was determined after Feulgen-staining with respect to the number of neutrophilic granulocytes. It was observed that nearly all mononuclear round cells exhibit diploid nuclear DNA content. No effect of the intensity of inflammation (as expressed by the content of neutrophilic granulocytes) was observed. From these results it is concluded that mononuclear cell proliferation is not influenced by the intensity of the inflammation. On the other hand it can be assumed that there is no immunological stimulus for an increassed proliferation of the synovial fluid in rheumatoid arthritis.

Arthritis, Rheumatoid↗
PubMed1977

[Pannus development in adjuvant arthritis of the rat - an autoradiographic study (author's transl)].

After the injection of complete adjuvant, rats develop arthritis with increased proliferation of synovial tissue cells at the attachment of the synovial membrane to cartilage and bone. From this synovial tissue a connective tissue pannus develops which covers the cartilage surface as a monocellular or multicellular layer of proliferating cells. Areas with cartilage destruction are usually characterized by a great number of proliferating connective tissue cells. Cartilage destruction is not restricted to the superficial pannus, since granulation tissue of the subchondral bone may also participate in cartilage resorption. The quantitative determination of 3H-thymidine labelled chondrocytes excludes participation of these cells in pannus formation.

Animals↗
PubMed1977

[Identification of plasmodial antibodies in the immunodiagnosis of malaria (author's transl)].

Out of 880 sera which were examined for antibodies to plasmodia 69 (7.8%) reacted only with P. falciparum antigen, 371(42.2%) reacted only with P. fieldi antigen, and 440 (50%) responded to both antigens. These groups were differentiated further with antigens from P. vivax, P. ovale, and P. malariae. The results showed that the P. ovale antigen was distinguished by a marked activity with those sera that had reacted only with P. fieldi in the screening test. With sera that had responded to both antigens or only to P. falciparum antigen in the screening test there was no such clear differentiation of the antibodies. P. falciparum was a less sensitive antigen for the demonstration of plasmodial antibodies (590, i.e. 57.8%, of the sera were positive) than P. fieldi (811, i.e. 91.2% of the sera were positive). The explanations given in the literature for the group and species specific reactions are discussed.

Antibodies↗
PubMed1977

The proliferation of chondrocytes and pannus in adjuvant arthritis.

Cell proliferation in the pannus formation of adjuvant arthritis was studied by autoradiography. It was found that after day 9 an increased cell proliferation starts in the joint capsule recessus and synovial villi on the injected side. From these proliferating cells a pannus, which during the first phase frequently consists only of few cell layers, extends over the cartilage surface. With advancing disease the thickness of the pannus increases and further centripetal growth may cause the entire cartilage surface to be covered. This proliferating pannus tissue may invade the cartilage and destroy it. Since in this area of destruction labelled cells are frequently present, it may be assumed that proliferating cells with a high enzyme content are particularly responsible for the immediate degradation of cartilage. No involvement of chondrocytes in pannus formation was confirmed by the methods employed. There was neither increased proliferation of surface chondrocytes nor increased proliferation of chondrocytes in the depth of cartilage.

Animals↗
PubMed1977

[Comparison of duck embryo vaccine and Hempt's vaccine for rabies vaccination (author's transl)].

For many years, Hempt's vaccine has been used in Germany for vaccination against rabies. In recent years the duck embryo vaccine has been gradually introduced in its place. From 1970 to 1975, 658 consultations were undertaken at the Hospital of the Tropical Institute at Hamburg because of the risk of rabies. 267 persons had to undergo vaccination, 159 of them being treated with Hempt's vaccine and 108 with duck embryo vaccine. Side effects of a more severe type were not seen in either group. Disadvantages of the duck embryo vaccine are that more individual inoculations are necessary, significantly more frequent and more persistent local and general vaccination reactions occur and that there is a delayed, possibly weaker formation of antibodies.

Animals↗
PubMed1977

[Immunological diagnosis of filariosis in persons returning from tropical countries and in European and indigenous inhabitants of endemic areas (author's transl)].

Serum samples from persons who lived in areas where onchocerciasis occurred or who had filariasis were examined with the complement fixation test and the indirect hemagglutination test for the presence of antibodies against crude extracts from Dirofilaria immitis, Onchocerca volvulus, Dipetalonema viteae, and Ascaris suum. The results could be interpreted as follows: 1. The indirect hemagglutination test was more sensitive than the complement fixation test for the demonstration of antibodies in sera from European and indigenous inhabitants of endemic areas. 2. There were no differences between the responses to the four crude worm extracts among the groups of 21 patients with Loa loa, 12 patients with Onchocerca volvulus, 11 patients with Dipetalonema perstans, and 22 patients with clinical filariasis in whom no microfilaria had been found. It was concluded that the examination with crude extracts cannot give any information about the antigens that had caused the stimulation of antibodies.

Adolescent↗
PubMed1977

[Cell image analysis on the PDP-12 computer: a software system for processing the image information from a scanning microscope photometer (author's transl)].

A system of computer programs is introduced which allows image analyzing of cytological or histological preparations. The software system was especially developed for a microprocessor (PDP-12, Digital Equipment Corporation) which is often implied in connection with microscope photometers (SMP 05, Carl Zeiss). Users need not to know anything about computer programming because the computer gives the necessary orders via CRT-display or printer. The different descriptors as they are available after different cytochemical or histochemical staining procedures are combined to a pattern vector which is to be a numerical equivalent of cell morphology. A novel method in numerically describing the fine structure of cell nuclei is emphasized. Different cell populations are compared by means of a simplified multivariate discrimiatory analysis. All programs are written in assembler language and they are stored on LINC tapes (Digital Equipment Corporation). Copies of system tapes respectively tapes with the source programs are available from the authors on sending of blank magnetic tapes (2,000 blocks marked). Description and print out of individual programs are published in the dissertation cited in References. Changes and additions of the system which have been done mean-while will be summarized, and lateron such summaries are available on request.

Cell Nucleus↗
PubMed1977

[Strain related differences in the immunodiagnosis of malaria (author's transl)].

In infections with P. falciparum and P. vivax antibody titers were found to differ in relation to strains. Various strains of both Plasmodium species used as antigens showed differences in their sensitivity in reactions with one patient serum. In P. vivax infections it is supposed that some antibody titer differences are caused by antigen variations.

Animals↗
PubMed1976

[Serum-antibodies in patients with extra-intestinal amoebiasis (amoebic liver abscess) and healthy persons (author's transl)].

Tests for serum antibodies against two amoeba antigens were performed on serum samples from 70 patients with amoebic liver abscesses, 204 travellers returning from tropical countries and 136 inhabitants of an endemic region without evidence of amoebiasis. Suspicious or borderline results in the complement-fixation, indirect haemagglutination, latex-agglutination, and double-gel diffusion tests occurred in 73.3%-90.4% of patients with amoebic liver abscess, in the same reactions (except double-gel diffusion test) in 12.1-18.9% of travellers in the trophics, and 16.2%-46.3% of inhabitants of endemic regions. There was evidence for the need of performing several tests: patients with amoebic liver abscesses gave diagnostically useful results the more frequently the more tests were performed on a given serum sample. Negative results in the three tests occurred in only one case of multiple liver abscesses, but the sample had been obtained very early.

Antibodies↗
PubMed1976

[Serodiagnosis of malaria (author's transl)].

Homologous and heterologous malaria pathogens were used as antigens for the determination of malaria antibodies by indirect immunofluorescence. Homologous antibodies fell in the second half year, after successful treatment, down to the limit of demonstrability. In inhabitants of endemic malarial areas, who lived in the German Federal Republic, signs of a fall in antibodies could be observed after about 30 months. The predominance of particular malaria pathogens of the country of origin was recognizable in the antibody spectrum of these persons. Malaria antibody findings of three comparable groups of exposed persons under chemoprophylaxis ought to provide pointers to the parasitemias which had occurred and which were held responsible for the antibody production. Persons without chemoprophylaxis from malarial areas served as controls. The markedly deviating results of the antibody findings in the serum donors can only be explained by more seldom parasitemias in persons with adequate chemoprophylaxis.

Adult↗
PubMed1976

[Recommendations for travel and residence in the tropics (author's transl)].

Increased tourism into tropical countries and the use of German labor forces in such countries make it necessary for doctors to advise emigrants properly. Besides vaccinations and drug prophylaxis, a rational way of life is extraordinarily important in the tropics, skin care, cleanliness, hygienic living, suitable clothing and sports activity are important factors to which attention must be paid. Additional salt is necessary if sweating is profuse. Alcohol should not be drunk before sundown. Examination before setting out for the tropics and after return for lesions and infections which may have appeared during residence in the tropics are urgently recommended.

Activities of Daily Living↗
PubMed1976

Skeletal muscle regeneration in myopathic hamsters of strain BIO 8262.

Skeletal muscle regeneration was studied in polymyopathic hamsters of the strain BIO 8262 by autoradiography. Flash labeling with 3H-thymidine revealed high labeling indices of mononuclear cells in areas with muscle cell necroses and low labeling of "interstitial cells". However, no labeled nuclei were present in myotubes. Three days after multiple injections of the radioisotope, labeled nuclei in the myotubes were present. The mean labeling intensity of these nuclei was slightly elevated on the 15th day. On the third day after multiple 3H-thymidine injections nearly all mononuclear cells were labeled; but their labeling indices slightly decreased until the 15th day. From the decrease of labeling intensity, a high turnover rate of these cells may be assumed. Labeled myotubes appeared in the neighborhood of mononuclear cells as well as in areas without pronounced round cell infiltrations. From labeled myotubes in areas with round cell infiltrations it may be concluded that they arose by fusion of rapidly proliferating satellite cells, which may be constituents of the mononuclear cell population. Labeled myotubes that appeared outside areas of mononuclear cell infiltrations may have been caused by fusion of slow proliferating satellite cells. Despite the fact of a high regeneration capacity of muscle in hamsters of the strain BIO 8262, this process is not sufficient to completely restore the muscle necroses.

Animals↗