Search PubMed⌕ Search

Biomedical subjects

W M Murphy

Publications and source records attributed to W M Murphy.

166 records · Page 10Linked to original sources
PubMedDate not supplied

Herpesvirus in bladder cancer.

A case of squamous cell carcinoma of the urinary bladder in which herpesvirus virions could be identified by electron microscopy is presented. The value of cytology in the recognition of the typical intranuclear inclusions which led to the initiation of further studies is emphasized. Recent evidence concerning the relationship between herpesviruses and human cancer is briefly reviewed. Although previous reports have emphasized the association of HSV and uterine cervical carcinoma, other combinations, especially with neoplasms of squamous cell type, have been reported. The accumulation of additional cases in which viruses can be demonstrated in cancers will help to define their role in human neoplasia.

Aged↗
PubMedDate not supplied

Morphologic effects of thio-TEPA on mouse urothelium.

The urothelial changes induced by intravesical instillation of thio-TEPA into experimental animals were studied by cytologic, light microscopic, and electron microscopic techniques. Cells and tissue from treated and untreated groups were compared using a total of 54 discriminators. There were few consistent differences. Cells with marked nuclear atypia were not identified. The results indicate that thio-TEPA has little effect on non-diseased bladders. The atypical cells occasionally present in specimens from thio-TEPA treated bladders apparently do no emanate from normal urothelium.

Animals↗
PubMedDate not supplied

The value of ethanol as a fixative in urinary cytology.

Although 50% ethanol is widely used as a fixative in urinary cytology, the value of this procedure has received little systematic analysis. The purpose of this study was to examine the preservative qualities of 50% ethanol when added to freshly collected urine for both immediate and delayed processing. The results indicate that: (1) degenerative changes occurring in vivo are common among urothelial cells and probably represent the single most important factor in cell preservation in voided urine; (2) with few exceptions, fresh, unfixed urine yields the greatest number of well-preserved cells; and (3) reconstitution in either saline or 25% ethanol-saline and 24-hour storage result in increased cellular degeneration. For Millipore filter preparations, the addition of 50% ethanol has no apparent advantage, even when processing must be delayed for several hours.

Cytological Techniques↗