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Biomedical subjects

W L Qiu

Publications and source records attributed to W L Qiu.

At least 37 records · Page 2Linked to original sources
PubMed2000

Hydroxy-dibenzo crown ether stationary phase for capillary gas chromatography using sol-gel technology.

A new kind of crown ether, OH-dibenzo-14-crown-4 (OH-DB14C4), is prepared and coated onto the fused silica capillary by sol-gel process. Chromatographic characteristics including column efficiency (> 3,000 plates/m), thermal stability (to 330 degrees C) and ability of deactivation are studied. The selectivity of new stationary phase is superior to sol-gel OH-terminal silicone oil (OH-TSO) for positional isomers of some aromatic compounds such as xylene, dichlorobenzene, nitrotoluene, nitrochlorobenzene. The new stationary phase has high sample capacity for separation of small molecular mass compounds: low-molecular-mass alcohols, ethers and ketones, short-chain fatty acids and volatile amines.

Chromatography, Gas↗
PubMed2000

[The Inhibitory Study of TNP 470 Combined CDDP on the Tumor Growth and Lung Metastasis of Adenoid Cystic Carcinoma ACC M in Nude Mice]

OBJECTIVE:To study the effect of TNP-470 combined CDDP on the tumor growth and lung metastasis of cell strain ACC-M in vivo. METHODS:High potential lung metastatic adenoid cystic carcinoma cell strains (ACC-M) 5x10(5) were injected simultaneously to 18 nude mice subcutaneously and enously. The animals were randomly divided into 3 groups. In group 1, TNP-470 was administered subcutaneously at the dose of 30mg/kg every other day for 2 weeks from the beginning of 3(rd) week to the end of 4(th) week. In group 2, 5mg/kg CDDP were injected into peritoneal cavity at the beginning of 3(rd) week and 4(th) week and TNP-470 in the same manner as group 1 additionally. In group 3, the same volume of 0.97% NaCl was injected in the manner of group 1. The mice were sacrificed by dislocating the neck at the end of 4 weeks. The tumor size and weights were recorded. The vascular density was measured by rabbit-anti-human factor VIII antibody stain. The results were analyzed by Student t and X(2) test. RESULTS:Group 1 and group 2 had fewer tumor weight and lung metastasis than the 3(rd) group. There was significant difference in the tumor weight and lung metastasis between group 1 and group 2. But the vascular density had no difference among three groups. CONCLUSION:TNP-470 combined CDDP has stronger inhibition on both the tumor growth and lung metastasis of human adenoid cystic carcinoma ACC-M than used alone.

Journal Article↗
PubMed2000

[Anatomical and histologic study of the cervical vessels in goats]

OBJECTIVE:To investigate the normal anatomic relations and histologic features of the cervical arteries and veins of goat, with the aim of providing a basis for resection and reconstruction of the common carotid artery.MATERIALS AND METHODS: Surgical dissection was performed on 15 healthy adult goats under general anaesthesia. The external diameters of the common carotid artery (CCA) and external jugular vein(EJV) were measured at their midpoints. 1 cm of the CCA and EJV was subject to light microscopic examination. Direct carotid angiography was performed on 2 selected goats to observe the course and branches of the carotid artery and normal blood flow mapping of the CCA was recorded using Laser Doppler Flowmeter. RESULTS:The average external diameter of the EJV was 5.4 mm for the left side and 5.3 mm for the right side. The average external diameter of the CCA was 3.8 mm for the left side and 3.6 mm for the right side. Histologic examinations found that the media of the carotid artery had 10-15 layers of smooth muscles. The EJV had valvulae, its wall was thin, only 1-2 layers of smooth muscle were contained in its medium. CONCLUSION:The CCA and EJV of the goat had a longer course in the neck, and a larger diameter (3-5 mm). Their histologic structures were similar to that of the human being, which makes it advantageous to be more often used as an animal model in experimental surgery.

Journal Article↗
PubMed1999

Evaluation of the treatment for micromandibular deformity by distraction osteogenesis with submerged intraoral device.

OBJECTIVE: To evaluate the results of distraction osteogenesis for correcting the micromandible with an intraoral device. MATERIALS AND METHODS: Eight cases of micromandible resulting from temporomandibular joint (TMJ) ankylosis and first and second branchial arch syndrome received treatment by distraction osteogenesis. Subjects included 5 females and 3 males, ranging in age from 10 to 43 years (avg 20.25). Five micromandibles were associated with obvious mandibular asymmetry. An osteotomy was performed on bilateral mandibular bodies by intraoral approach. Elongation was started on the 5th or 6th day postoperation at a rate of 0.8 to 1 mm per day. The distractors were removed after a consolidation period from 71 to 139 days. RESULTS: The distractions were fulfilled with a range of 8.5 to 24.4 mm. Pain appeared in osteotomy region when the 1 mm distraction was carried out once a day and disappeared when it was divided into twice a day. Mandibular lengthening was successful. Micromandible and facial asymmetries were corrected satisfactorily. The follow-up period ranged from a minimum of 1 month to a maximum of 10 months. There was no recurrence. One case of soft tissue infection and one case of lower lip numbness were reported. There were no cases of infection or other disturbance of wound healing or pseudarthrosis. CONCLUSIONS: Because of its advantage in little injury, avoiding bone grafting, and few complications, intraoral distraction osteogenesis is a valuable approach to correct mandibular agenesis. However, in the management of mandibular deformities, distraction osteogenesis achieves better results when combined with other orthognathic operations. How to control the direction of distraction is a problem that needs further study.

Adolescent↗
PubMed1999

[Early face mask treatment for maxillary hyperplasia in patients with cleft lip and Palate]

OBJECTIVE: Early interfering orthodontics were used to treat maxillary hyperplasia by face mask and extra-oral bow technique in teenages.The position changes of maxilla after orthodontic treatment were evaluated.METHODS:The cephlometric variables before and after treatment were measured.Variables after treatment were compared with normal values in children with same ages,and changes of the maxillary position was determind.RESULTS:The position of the maxilla after orthopedics was significantly advanced (P<0.001),but there was still difference from normal values.CONCLUSION:In the peroid of mixed dentition of patients with cleft lip and palate,early orthodontics on maxillary hyperplasia could stimulate maxillary development and growth,remove or improve crossbite,extra-oral bow and face mask were efficient tools for this kind of patients.

Journal Article↗
PubMed1999

[A preliminary cephalometric study on characters of facial soft tissue in mandibular asymmetry cases]

OBJECTIVE: To study the alterations of facial soft tissue in mandibular asymmetry cases.METHODS:The asymmetric rates of the bone were compared with that of soft tissue by cephalometric analysis in 20 mandibular asymmetry cases by study on the thickness of the soft tissue in special position.RESULTS:It showed that the soft tissue is more symmetric than the bones.CONCLUSION:It can be concluded that the facial soft tissue is not as asymmetric as the bone.This suggests that the reconstrction of the bony deformities and the soft tissue deformities should not be performed at same time.

Journal Article↗
PubMed1999

[The changes of chewing ability after orthognathic therapy: electromyographic analysis]

OBJECTIVE:The maxillo-facial skeletal deformity can only be corrected through orthognathics that consists of orthodontics and operation.There are various changes in aspect of the bite relationship between upper and lower dentition after orthognathic surgery.The change feature of the chewing efficiency of oral maxillary system can be detected through comparing studying of the contracti on force of chewing muscles before and after surgical operation.METHODS:All 6 patients in this research were diagnosed to be skeletal crossbite.Electromyogram was used to test the change of activity of superficial masseter,temporalis and digastrics.The examination was carried out 1 week before operation and 3 months after the operation. RESULTS:When mandibular is in its rest status,there is no significant difference in the change of muscular activities.when it is active,there is significant difference. CONCLUSION:The chewing ability of masticatory muscles is reduced after orthognathic surgery.The result indicated that there is a negative bite relationship between the upper and lower dentition,the occlusion relationship needs accurate adjustment for gaining stability of oral maxillary system.Therefore,orthodontics after operation is of vital importance.

Journal Article↗
PubMed1999

[The in vitro comparison of platelet induced ACC-M cell adhesion with and without Aspirin]

ObJECTIVE:Observation the affection of aspirin to adhesion of highly metastatic salivary adenoid cystic carcinoma(ACC-M) cell induced by platelet.METHODS:Adhesive ratios of ACC-M cell induced by platelet with or without aspirin were measured.RESULTS:After the addition of platelet rich plasma(PRP),adhesive ratio of ACC-M cell increased significantly (P<0.01). The treatment with aspirin after addition of PRP led to a nonsignificant increase of adhesive ratio (P>0.05).The reduction of adhesive ratio by aspirin was found dosage independent within concentration range of 0.01-0.25g/L of aspirin. CONCLUSION:Adhesion of ACC-M cell was raised by added platelet.Addition of aspirin,platelet induced adhesion of ACC-M cell was inhibited significantly.

Journal Article↗
PubMed1999

[Evaluation of cryosurgery for the management of oral malignant mucomembranous melanoma-A survey of 107 cases reports]

OBJECTIVE:There were 13 cases underwent operation.24 cases underwent cryosurgery only and 70 cases received comprehensive management,which as cryosurgery,operation,chemotherapy and immuno-therapy.RESULTS:The 3'and 5' year survival rate of three groups were 0.0%,0.0%,37.50%,31.25% and 57.14%,36.07%.There are significant differences between operation group and cryosurgery group and between operation group and comprehensive treatment group.Although the third group got the better result,but the difference between late two groups was not statistical significant.CONCLUSION:It is suggested that comprehensive treatment be the routine regime of melanoma in head and neck. Our experience with review of literature covered the classification, surgical principle and complication was presented.

Journal Article↗
PubMed1999

[Presurgical infant orthopedics in complete bilateral cleft lip and palate reconstruction]

OBJECTIVE:This study want to effects on using intraoral-appliance treatment of complete bilateral left lip and Palate in clinic.METHODS:Using Lisson's method preliminary evaluated to effect of presurgical infant orthopedics complete bilateral cleft lip and palate.A sample of 4 cases of 18 weeks infant complete bilateral cleft lip and palate.2 of they are males, others are females.All of they had received presurgical infant orthopedics,Using intraoral appliance. comparison of before and after cast analysis.Evaluate anteiro posteior I, tranverse,oblique degree of the lateral segment, pretrusion premaxilla position change.RESULTS:Every patients after orthopedics treatment had retracted premaxilla come back,corrected of oblique of premaxilla,had lateral segments growth equally on both side,their inclination should be similar.CONCLUSION:Intraoral-appliance can successfully improved malarrangement maxilla of bilateral cleft lip and palate.

Journal Article↗
PubMed1999

[Detection of clinical diagnosis and different symptoms of Sjogren's syndrome by traditional chinese medicine (TCM)]

OBJECTIVE:To search for the appropriate diagnostic criteria of SS and confirm the differentiation of SS.METHODS:The European di agnostic criteria has been used in clinic work for about two years,and differentiated the symptoms of SS by TCM. The specialty and relationship between them was made an inquiry.RESULTS:The European criteria set for SS offers the clinic work a practical method to correctly classify the patients suspected of having this disorder. It refers to the signs and symptoms caused by the insufficiency of spleen-qi with deficiency of liver-yin and kidney-yin.CONCLUSION:It has been further proved for easy to make up the diagnostic of SS and its treatment using traditional chinese medicine.

Journal Article↗
PubMed1999

[Experimental research on animal model and treatment of Sjogren's syndrome by traditional chinese medicine (TCM)]

OBJECTIVE:To confirm the function of SS Syrup used in our department. METHODS: Under the basis of TCM and differentiation symptoms of SS,the animal model was established and applied to test the function of SS syrup.There are three indices to be observed,weight change,volume of drinking water daily,cAMP change. RESULTS: The three observed indices of dificiency symptom model had been rectified by SS syrup. CONCLUSION: SS syrup has two main functions:(1)reinforcing of the spleen and tonifying qi;(2)nourishing liver-yin and kidney-yin as well as removing pathogentic heat and fire.

Journal Article↗
PubMed1999

[Primary research of development of a mouse model for non-bacterial sialoadenitis]

OBJECTIVE: To develope a mouse model for non-bacterial sialoadenitis similar to SS.The model of organ-specific to the submandibular gland of mouse has developed for this study. METHODS: Several of the mouse strains were used to the development of non-bacterial sialoadenitis.The injection of syngeneic salivary gland tissue with CFA induced inflammation in submandibular gland in mice.Not all mice had a predictable response. RESULTS: C(57) mice (500microg/ml and 750microg/ml group )appear histologically to be more prove to develop sialoadenitis. CONCLUSION: An animal model of non-bacterial sialoadenitis had been set up,the non-bacterial sialoadentitis may be similar to the autoimmune process in human SS.

Journal Article↗
PubMed1999

[Observation in vivo histological response induced by imlplanted domestic poly-DL-lactic acid plates]

OBJECTIVE:To analyze the histological response and biocompatibility to implanted domestic PDLLA plates in vivo METHODS:Bilateral artificial fractures were created in mandibular bodies in dog and internal fixed with domestic PDLLA plate,TM plate respectively;meanwhile,a domestic PDLLA plate was imlplanted in femur.The interfacial soft tissue around the plates were collected and performed histological study RESULTS:All the skin wound healed well without abscess and fistula formation. From 2 to 4 weeks after the operation,the inflmmatory granulomatous response around the intenal fixation PDLLA plates was more serious than that of the control,in which more macrophages,plasmocytes and less lymphocytes,mononuclear cells infiltrated CONCLUSION:Domestic PDLLA plate is an inert internal fixation material with good biocompatibility, which induced a non-speciflc foreign reaction

Journal Article↗
PubMed1999

[Observation of the biodegradation of domestic poly-DL-lactic acid plates in vivo]

OBJECTIVE:To analyze the degradation and related factors of domestic poly-DL-lactic acid(PDLLA) plates in vivo. METHODS: The artifical transverse fractures in mandibular bodies of dogs were created and internal fixed with domestic PDLLA plates as experimental group,the implanted PDLLA plates in femurs as control.A certain time after the operation,the plates in fractured and implanted regions were collected and undertaken macroscopy and scanning electromicroscopy(SEM),meanwhile the fracture-healing status was evaluated. RESULTS: With time going on,the surface of domestic PDLLA plates y and density of micro-holes among poly-DL-lactic acid molecules in the PDLLA plates increased gradually.16-20 weeks Domestic PDLLA plates can be gradually biodegradation in vivo,in which it is correspondent with and accelerative the fracture-healing process.

Journal Article↗
PubMed1998

Experimental study of radiation effect on the mandibular microvasculature of the guinea pig.

OBJECTIVE: This experimental study was performed to explore the intraosseous microvascular alterations in the irradiated mandible and to increase understanding of the pathogenesis of osteoradionecrosis. METHODS: One hundred twenty-eight guinea pigs were grouped according to different radiation sources and dosages. Fractioned radiation was delivered to the right mandibles. Dental extractions were conducted at 1, 3, and 5 months respectively, after radiation. One month later, the animals were examined by means of gross observation, histopathology, and microvascular corrosion castings. RESULTS: Histologic evaluation showed bone absorption within 2 months following radiation. Four months later, the number of osteocytes decreased and pyknosis and empty lacunae were commonly seen. The casting specimens revealed under scanning electron microscopy that capillary disconnection and obliteration with subsequent vein shrinkage aggravated over time. In the nonhealing dental extraction site, a large-scale vascular network defect presented with focal capillary proliferation around. CONCLUSIONS: The capillary is the part most vulnerable to the damage caused by radiation in the vascular system of the mandible. On the basis of "hypovascular, hypoxic, hypocellular" structure, local microcirculation failure induced by the trauma-repairing process leads to occurrence of osteoradionecrosis of jaws.

Animals↗
PubMed1998

[A comparison of the effects of 180kv X-ray radiation and cobalt-60 radiation on the jaws of the guinea pigs]

OBJECTIVE: Study of the effects of 180kv x-ray and cobalt-60 radiation on jaws and enhance the understanding of pathology of osteroradionecrosis of jaws(ORNJ).METHODS: 128 guinea pigs were grouped according to different irradiation sources and doses.Fractioned radiation from both sources was delivered in right mandible.The teeth extractions were conducted one week prior to radiation and 1,3,5 months after radiation respectively.Following radiation,the guinea pigs were examined by gross observation, x-ray film and histopathological examination at regular interval. RESULTS: Bone resorption occured within 1 month after irradiation as result of osteoblast inhibition and osteoclast activation.From 2 to 4 month afterwards,the number of osteocytes reduced and pyknosis, empty lacunae as well as obliteration of small vessels in periodontal membrane were commonly seen.The teeth extractions conducted at 3 months,5 months after radiation lead to ORNJ in 7 amimals (14.6%).CONCLUSION: 180kv x-ray radiation was far more damaging to jaws than cobalt-60 radiation.The dental extraction is an important factor inducint the development of ORNJ.

Journal Article↗