Search PubMed⌕ Search

Biomedical subjects

W Kraft

Publications and source records attributed to W Kraft.

At least 37 records · Page 2Linked to original sources

[Age dependency of laboratory values in dogs and cats. II. Electrolytes in serum].

Significant age dependent differences in dogs were found in sodium, potassium, calcium, chloride, and inorganic phosphorus, whereas in cats only potassium, chloride, and inorganic phosphorus were found to be age dependent. In both species special reference values must be established for different age groups. The higher reference values of potassium in young dogs and cats should be considered in cases of rehydration.

Aging↗

[Percutaneous cystoscopy in dogs and cats].

Cystoscopy enables a reliable diagnosis of urinary bladder diseases. The use of transurethral or perineal endoscopic technique is limited by anatomical features. Therefore it was examined, if the prepubic percutaneous access shows advantages referring to diagnosis and treatment. Using this technique three dogs and two cats were examined. The endoscopic instrumentarium and the technical procedure are described.

Animals↗

[Determination of canine thyroid stimulating hormone (cTSH) in blood serum of dogs and its reaction to the TRH stimulation test].

The introduction of the determination of canine TSH in the blood serum of the dog means a substantial enrichment in the diagnosis of thyroid disease. In the present study the basic value of serum c-TSH and its maximal increase after intravenous injection of 200 micrograms TRH per dog were determined. The basic value was 0.18 +/- 0.11 ng/ml, the maximal increase was reached between 15 and 30 minutes after injection of TRH and was 0.53 +/- 0.24 ng/ml. In two hypothyroidal dogs the basic values of T4 were 1.1 and 0.56 microgram/dl and of fT4 0.19 and 0.15 ng/dl, respectively. Their TSH concentration was 1.35 and 4.0 ng/ml, respectively.

Animals↗

A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces.

A polymerase chain reaction (PCR) assay is described for the detection of parvovirus in feces of dogs and cats. A touch-down protocol was used which enabled the specific amplification of virion DNA from feces after a fast and simple boiling pretreatment. The sensitivity of PCR was as high as ten infectious particles per reaction which corresponds to a titer of about 10(3) infectious particles per gram of unprocessed feces. This renders the PCR about 10- to 100-fold more sensitive than electron microscopy, the standard method for parvovirus diagnosis. The very rapid and simple sample preparation recommends this PCR assay as an alternative technique for routine parvovirus diagnosis.

Animals↗

[Clinical veterinary medicine in Germany--quo vadis?].

The project to reorganize the standards for the veterinary study in Germany deviates considerably from the former regulations and those devised by the Association of German Veterinary Faculties. The standards will result in significant effects on the future development of the German veterinary profession. For the clinical education a distinct change to the worse will be expected with the consequence of a diminished qualification of the following generations of German veterinarians compared with the European and American countries.

Education, Medical↗

[Domestic animals as patients in a small animal practice. 2. Handling, sex determination, blood collection, drug application].

The importance of small pets as domestic animals and patients in veterinary practice increased in the last years. Differences in physiology and behaviour require exact knowledge of appropriate handling and sex determination, techniques of blood collection as well as drug application methods. Therefore, a survey of rabbit, guinea-pig, chinchilla, hamster, gerbil, mouse, and rat as patients in veterinary practice is given.

Animals↗

[Clinical symptoms and diagnosis of feline infectious peritonitis].

Body effusions from 197 cats and blood serum samples from 252 cats, where Feline Infectious Peritonitis (FIP) was part of the differential diagnosis, were analysed. The diagnoses were confirmed by clinical follow up or histopathology. The final diagnosis FIP was always confirmed by histopathology. The median age of cats with FIP was 1.6 years. FIP was responsible for 41% of the body effusions, whereas malignomas caused 24%, cardial insufficiencies 14% and purulent serositis 12% of the body effusions. The rivalta test was highly sensitive for FIP. Predictive value of a negative result was 100%, predictive value of a positive result was 84%. In half of the cases with purulent serositis and in 20% of malignomas rivalta reacted positive. The cardial insufficiencies were negative for rivalta. Coronavirus antigen could be demonstrated by immunofluorescence in 34 of 49 body effusions caused by FIP, whereas in the 50 body effusions caused by other diseases no coronavirus antigen was detected. An albumin globulin ratio of < 0.6 was highly diagnostic for an inflammatory process, nearly exclusively for FIP. An albumin globulin ratio of > or = 0.8 almost excluded FIP. Only a negative or very high (1:1600) FIP titer could contribute to confirm diagnosis. Low and medium titers, however, should not be interpreted.

Aging↗

[Rapid enzymatic test for diagnosis of parvovirus infections in dogs].

In this study 52 canine fecal samples were examined for the presence of canine parvovirus (CPV). The two different test systems used to confirm infection were electron microscopy as standard method and an enzyme-linked immunosorbent assay (ELISA). In comparison, the results conferred in 92.3%. The CPV ELISA had a sensitivity of 89.7% and a specificity of 100%. Therefore, it can be used as a quick, reliable method for diagnosis of canine parvovirus infection in veterinary practice.

Animals↗

[Dependency on age of laboratory values in dogs and cats. 1. Enzyme activities in blood serum].

A significant dependency on the age of dogs can be observed in the following serum enzymes: AST, GLDH (low degree), SAP, GGT, and CK. In cats a dependancy on age can be seen in ALT, AST, SAP, LDH, alpha-HBDH, alpha-Amylase, Lipase, and CK. The differences between old and young animals are small in AST, GLDH, GGT, and LDH. Therefore, the differences are not important for routine diagnosis. Comparing ALT activities, lower values were found in kittens. Similar results were found in CK activities. On the other hand higher values of SAP can be seen in young than in adult individuals. In geriatric animals, diagnostically important changes of the enzyme activities examined in this study could not be found.

Aging↗

[Treatment of severe neutropenias in dogs and cats with filgrastim].

The recombinant hG-CSF Filgrastim was used in severe cases of neutropenia in the dog caused by parvovirosis, hyperestrogenism, treatment with antineoplastic agents, an aplastic syndrome and in the cat in cases of infectious panleukopenia. Increases of the numbers of leukocytes were observed in all groups of diseases in the dog, but not in feline infectious panleukopenia. Filgrastim is indicated in neutropenias associated with disturbance of the general condition accompanied by fever, but not in cases of transitory leukopenias.

Anemia, Aplastic↗

[Alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH), alkaline phosphatase (AP) and gamma-glutamyltransferase (GGP) in intestinal diseases of dogs].

331 dogs, suffering from different intestinal diseases with diarrhea, were classified into the groups of "acute noninfectious", "infectious", "chronic" and "secondary enteropathies". The serum enzymes ALT, AST, GLDH, AP and GGT were determined. In all groups increases of enzyme activities were to be found. The highest and most frequent increases have been observed in acute noninfectious and in secondary enteropathies. The enzyme pattern in acute noninfectious enteropathies indicate a secondary liver disturbance in consequence of the intestinal disease, whereas the liver participation in secondary enteropathies is the effect of the primary disease other than intestinal disturbances. In comparison to this the height and number of increases of liver enzyme activities were low in acute infectious and in chronic enteropathies.

Alanine Transaminase↗

Atrial natriuretic peptide and plasma volume of dogs suffering from heart failure or dehydration.

Studies in humans and experimental animal models suggest that volume overload increases and volume underload decreases release of atrial natriuretic peptide (ANP), a hormone intimately linked to water and electrolyte homeostasis. This relationship was examined in dogs suffering from heart failure or dehydration and the data presented here are in support of this general concept. Plasma ANP concentration in dogs with congestive heart failure (CHF) was elevated with the severity of the disease (NYHA classification, class II: 21.4 +/- 9.2 fmol ANP/ml; class III: 65.5 +/- 72.6; class IV: 119.7 +/- 87.1; healthy dogs: 13.9 +/- 7.5). The increment in plasma ANP concentration in cardiac patients was also positively correlated with the plasma volume. The blood volume of dogs with moderate and severe CHF was significantly (P < 0.05) elevated and a normotonic blood pressure prevailed. In contrast, dehydrated dogs tend to display reduced ANP plasma concentration (7.7 +/- 5.6 fmol/ml) as well as significantly lower plasma volume and reduced blood pressure (P < 0.05). In dogs with severe CHF, ANP precursor material is present in the blood, which is normally undetectable. These data further support the concept of a regulatory function of ANP in volume homeostasis of dogs.

Animals↗