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Biomedical subjects

W James

Publications and source records attributed to W James.

At least 73 records · Page 4Linked to original sources
PubMed1985

spoVIC, a new sporulation locus in Bacillus subtilis affecting spore coats, germination and the rate of sporulation.

A mutation near cysB on the Bacillus subtilis chromosome marks a new sporulation locus, spoVIC. It causes spores to germinate more slowly than those of the wild-type under all conditions and, from indirect evidence, it does not appear to alter the affinity for the germinant L-alanine. The mutant spores have some deficiency of coat proteins (particularly the alkalisoluble coat protein, Mr = 12 000) and the spore coat layers are disorganized. The mutant strain grows normally and sporulates normally until stage II, after which its sporulation is delayed by about 2 h compared to that of the wild-type. This delay results in the prolonged synthesis of some coat proteins and the late synthesis of others. The abnormal coat may be the cause of the germination deficiency. A double mutant strain carrying the spoVIC610 mutation together with gerE36 sporulates slowly. Its spores have very little coat protein, are sensitive to heat, lysozyme and organic solvents, but germinate as well as the strain carrying the spoVIC mutation alone. The role of the spore coat in germination is discussed in the light of these findings.

Bacillus subtilis↗
PubMed1985

Protease production during sporulation of germination mutants of Bacillus subtilis and the cloning of a functional gerE gene.

Early in sporulation, cells of wild-type Bacillus subtilis produce three proteases (b, c and d) with monomeric Mr values of about 65 000, 53 000 and 43 500, and a further protease, e (Mr about 30 000) at the time of coat assembly. An additional protease, f (Mr about 15 000) appears transiently in sporangia at about the time of spore release. Three strains with defective spore coats were examined for alterations in sporulation proteases. A strain carrying the gerE36 mutation produces b, c and d normally, fails to produce e and accumulates f on or in its spores. A strain carrying the spoVIC610 mutation produces normal quantities of proteases b, c and d, but has a reduced amount of proteases e and f. A strain carrying both the gerE36 and the spoVIC610 mutations accumulates neither protease e nor f. The wild-type allele of the gerE gene was cloned in the vector, phage phi 105J9. Complementation tests with the cloned gene showed that the gerE36 mutation is recessive to the wild-type allele.

Bacillus subtilis↗
PubMed1984

A comparative study of aerosolized terbutaline and subcutaneously administered epinephrine in the treatment of acute bronchial asthma.

Patients with acute asthmatic attacks were prospectively randomized to receive either aerosolized terbutaline or subcutaneous epinephrine. After treatment there was comparable improvement in pulmonary function in both groups after two, 30 and 60 minutes. There was a significant increase in pulse rate with epinephrine that was not seen with terbutaline.

Acute Disease↗
PubMed1982

Urethral insertion of foreign bodies. A report of contagious self-mutilation in a maximum-security hospital.

Six male patients in a maximum-security hospital committed acts of urethral self-mutilation by insertion of a foreign body. The characteristics of the self-mutilators and of this unusual form of self-mutilation are described. The social contagion aspects of the self-mutilation are strongly suggested by the perpetration of urethral insertion for the first time by five patients after direct personal contact with a previous urethral self-mutilator. Techniques used in the management of these patients are described. Self-mutilation evokes strong emotional responses in staff members, and proper patient management also must include attention to the needs and concerns of treatment staff.

Adult↗
PubMed1976

Six unusual cases of drug overdose.

Six patients out of a total of 1,137 adults treated for drug overdose died during the period May, 1968 to June, 1973. Their case histories are summarized, and the conclusion drawn that, whilst death from drug overdose is unusual, increased vigilance particularly for the unsuspected secondary diagnosis or complication, is needed if there is to be any further reduction in the already small mortality rate.

Adult↗
PubMed1976

Grey-scale ultrasonic imaging of the kidney.

The ultrasonic appearances of the kidney in images which contain grey shades corresponding to the detected echo-pulse heights have been examined. These "greyscale" images were obtained using a standard Nuclear Enterprises Diasonograph 4102B and a Varian 620/L digital computer interfaced to this Diasonograph. The computer was used to analyse and display the ultrasonograms with 16 grey shades. Both kidneys of 36 subjects were examined and compared with IVU findings. This group included patients with normal kidneys, polycystic kidneys, hydronephrosis, glomerulonephritis and neoplastic tumours. It was found possible to make sharp, grey-shaded ultrasonograms which were very useful to the diagnostician. In particular, the grey shade of the renal parenchyma was used as a standard to compare other grey-shaded structures. Also the grey shading was useful in defining the renal pelvis in transverse scans and the renal poles in axial scan.

Glomerulonephritis↗
PubMed1975

A comparison of thermography, radioisotope scanning and clinical assessment of the knee joints in haemophilia.

Thermography, radioactive scanning and clinical assessment of the knee joints of a series of patients with haemophilia or Christmas disease have been performed. A positive correlation was found between the abnormalities of the thermograms, radioactive scans and the clinical signs in acute haemarthrosis, but not in chronic haemophilic joint disease nor in patients without clinical joint disease. No correlation between the thermograms, radioactive scans and the number of previous joint bleeds was shown. Thermography and radionuclide scanning provide rapid means of quantifying changes within the haemophilic joint and may be useful in assessing treatment of haemophilic haemarthrosis.

Adolescent↗