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W J Judd

Publications and source records attributed to W J Judd.

At least 73 records · Page 4Linked to original sources
PubMed1977

Anti-Lu14: a Lutheran antibody defining the product of an allele at the Lu8 blood group locus.

A 'new' Lutheran-related antibody, named anti-Lu14, reacts with approximately 2.4% of random bloods. Red cells of the rare Lu:-8 phenotype are Lu:14. The data indicate, with a high probability, that the Lu 14 antigen is a product of an allele of Lu8 and that Lu14 and Lu8 comprise a third pair of alleles at the Lutheran locus. Red cells of the original Sw (a+) propositus are Lu:14. By coincidence, he has inherited two low-incidence genes. This observation may explain the discrepancy in different families concerning a possible relationship between Swa and Lutheran. Pedigree information now suggests that Swa is not a Lutheran gene.

Alleles↗
PubMed1977

BS II lectin: a second hemagglutinin isolated from Bandeiraea simplicifolia seeds with affinity for type III polyagglutinable red cells.

BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B-like' antigens present on 'acquired B' red cells. Two other blood samples which did not possess 'acquired B' antigens were also found to react with BS II lectin. Both of these samples were shown to have properties similar to those reported for Tk red cells. The serological characteristics of red cells modified in vitro by a culture broth of Bacteroides fragilis suggest that type III polyagglutinable receptors are identical to those associated with Tk red cells.

ABO Blood-Group System↗
PubMedDate not supplied

A pH-dependent auto-agglutinin with anti-P specificity.

An agglutinin with apparent anti-P specificity, reactive only at pH values below 6.0, was found in the plasma of a healthy female blood donor. It is postulated that this agglutinin recognizes latent receptors revealed by structural alterations to red blood cell membranes which occur in an acid environment.

ABO Blood-Group System↗
PubMedDate not supplied

An anti-B reagent prepared from the alpha-D-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds.

A method for the large scale preparation of the alpha-D-galactosyl binding isolectins from Bandeiraea simplicifolia seeds using an absorbent prepared by linking D-galactosamine to CH-Sepharose is described. The addition of N-acetyl-D-galactosamine (GalNAc) to these isolectins produced an anti-B reagent (BS I + GalNAc anti-B). Although BS I + GalNAc anti-B readily agglutinated red blood cells from the majority of group B and AB donor and patient blood samples tested, it reacted weakly with group B and AB cord red blood cells and failed to agglutinate five of 100 group AB donor blood samples when tested by an automated technique. The reagent did not agglutinate the red blood cells from seven acquired-B red blood cell samples and was strongly reactive with Tn-polyagglutinable red blood cells. These findings indicate that this lectin anti-B preparation may be of more value in investigative immunohematology, rather than as an alternative to human group A serum as a source of anti-B for blood-typing purposes. The results of tests on Tn-polyagglutinable red blood cells with BS I + GalNAc anti-B and the purified isolectins BS I(A4) and BS I(B4) are also presented, and discussed in relation to current concepts on the structure of the Tn receptor.

ABO Blood-Group System↗
PubMedDate not supplied

Anti-lea as an autoantibody in the serum of le(a - b+) individual.

The first example of anti-Lea, as an autoantibody, in the serum of a phenotypically Le(a-b+) individual is described. The patient is a 55 year old male with squamous cell carcinoma of the esophagus. His saliva contains Lea, Leb and H substances, and inhibits the hemagglutinating activity of his serum anti-Lea. The Leb antigen content of his red blood cells is approximately half that of control Le(a-b+) red blood cells. The patient's disease process may be responsible for the development of this unexpected autoantibody.

ABO Blood-Group System↗
PubMedDate not supplied

Antibodies that define NANA-independent MN-system antigens.

The hemagglutinating properties of a large proportion of anti-M and anti-N reagents, and sera containing antibodies to MN-related antigens, have been shown to be unaffected by treatment of red blood cells with neuraminidase. These antibodies, which define NANA-independent MN-system structures, provide further evidence that MN blood group specificity may also be determined by moieties other than N-acetylneuraminic acid.

Antigen-Antibody Reactions↗
PubMedDate not supplied

Concomitant T- and Tk-activation associated with acquired-B antigens.

Three patients with acquired-B antigens are reported whose red blood cells also manifested T- and Tk-activation. The results of tests using purified Arachis hypogaea and Bandeiraea simplicifolia (BS II) lectins, and red blood cells either T, Tk or both T and Tk-activated by bacterial enzymes in vitro, suggest that the acquired-B phenomenon, T-activation and Tk-activation result from the action of different bacterial enzymes.

ABO Blood-Group System↗
PubMedDate not supplied

The use of purified lectins in immunhematology.

A simple method for preparing purified lectins for use in immunohematology, employing bovine albumin as a diluent, is described. The results of tests using purified lectins on normal, polyagglutinable and selected MN-system variant red blood cells, confirm and extend previously reported results obtained with impure lectin preparations.

ABO Blood-Group System↗
PubMedDate not supplied

The rapid identification of Chido and Rodgers antibodies using C4d-coated red blood cells.

A simple method for the recognition of anti-Ch and anti-Rg, using red blood cells coated in vitro with C4d, is described. Of 58 anti-Ch and 20 anti-Rg sera studied, all caused direct agglutination of C4d-coated red blood cells in tests incubated at room temperature. Moreover, selected sera were shown to react strongly (3+ to 4+) with C4d-coated red blood cells when tested by the indirect antiglobulin test without prior incubation at 37 C. Sera containing other high-titer, low avidity (HTLA) antibodies were either nonreactive with C4d-coated red blood cells, or reacted to the same degree with "non-C4d-coated" trypsin-treated red blood cells. Similar findings were obtained in tests with non-HTLA antibodies of assorted specificities, including a variety of alloantibodies to high-incidence red blood cell antigens. These findings indicate that the use of C4d-coated red blood cells in investigative immunohematology may aid in the rapid identification of Ch and Rg antibodies.

Antibodies↗
PubMedDate not supplied

Paraben-associated autoanti-Jka antibodies. Three examples detected using commercially prepared low-ionic strength saline containing parabens.

Three examples of autoantibodies with Jka specificity are reported. They all react preferentially by low-ionic strength salt (LISS) techniques, but only in the presence of parabens (butyl, ethyl, methyl, and propyl esters of p-hydroxybenzoate) or certain other neutral aromatic compounds. They were detected through the use of commercially prepared LISS solution containing parabens as preservatives. While it is unlikely that these autoantibodies are of clinical significance, the parabens can now be added to the list of reagent preservatives that may give rise to anomalous serologic reactions.

Adult↗