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Biomedical subjects

W Hoppe

Publications and source records attributed to W Hoppe.

At least 55 records · Page 3Linked to original sources
PubMed1981

[Whipple disease of the CNS].

By the example of two cases that could only be cleared up by a postmortem examination the affection of the central nervous system by Whipple's disease is dealt with. The granulomatous inflammatory process which is almost exclusively localised in the grisea and shows a particular concentration in the diencephalon, in the cortex of the frontal, temporal and islet regions as well as in the deeper brain stem, had resulted in a variety of neurological-psychiatric symptoms, in most cases a systemic psychosyndrome, myoclonism and paresis of ocular movement. In both cases the diagnoses was verified by the electromicroscopic identification of the bacteria in the typical SPC cells.

Adult↗
PubMed1980

The core subunit structure in RNA polymerase holoenzyme determined by neutron small-angle scattering.

The core subunit arrangement of alpha 2-beta-beta' within DNA-dependent RNA polymerase holoenzyme alpha 2 beta beta' sigma from Escherichia coli was investigated by neutron small-angle scattering using label triangulation. The quaternary structure of multisubunit biomolecules can be studied by this new method if total reconstitution works in a quantitative way and if extensive replacement of C-bound hydrogen (H) by deuterium (2H) is possible. A substitution of the selected subunits by their fully deuterated analogues was used for the analysis of the overall shapes of the core subunits, alpha 2, beta and beta' in situ and for the determination of the intersubunit centre-to-centre distances. The contrast between the buffer and the remaining 'hydrogenated' enzyme vanishes if the buffer contains 42% 2H2O (matching of scattering length densities). The isotopic hybridization of the enzyme fulfils the conditions of isomorphous replacement as required: molecular functions, like enzyme activity, were completely preserved. The orientations of the core subunits within the holoenzyme were derived by comparing theoretical and experimental pair distance distribution functions, P(r), obtained from the scattering intensity differences of the pair-labelled (e.g. both beta and beta' labelled) and both mono-labelled molecules by direct Fourier transformations. Additional, the subunit shapes were refined by P(r) analyses. The arrangement of the stable core structure within the holoenzyme, which contains sigma as a dissociable factor, is presented in a three-dimensional model.

DNA-Directed RNA Polymerases↗
PubMed1980

The subunit positions within RNA polymerase holoenzyme determined by triangulation of centre-to-centre distances.

The complete 'centre-of-subunit structure' of the multisubunit enzyme DNA-dependent RNA polymerase was determined by triangulation of the subunit positions using the intersubunit distances calculated from scattering difference measurements and from the corresponding radii of gyration R. In addition to the centre-to-centre distances d between the core subunits alpha 2, beta and beta' presented in the preceding paper, the values of d between initiation factor sigma and alpha 2 (8.4 +/- 1.6 nm), beta (4.4 +/- 2.2 nm) and beta' (10.7 +/- 1.5 nm) were derived from R of sigma (4.1 +/- 0.3 nm) in situ and of the pairs alpha 2--sigma (6.1 +/- 0.4 nm), beta--sigma (5.6 +/- 0.3 nm) and beta'--sigma (7.5 +/- 0.4 nm) within the holoenzyme (alpha 2 beta beta' sigma). The structural parameters of the subunits within their molecular complex are accessible for neutron small-angle scattering measurements using labelling of the different subunits (deuteration), total reconstitution of isotopic hybrids, scattering length density matching of 'hydrogenated' molecular parts and extended exposure times because of weak scattering effects. The overall shape of sigma bound to core enzyme (alpha 2 beta beta') proved to be identical (within experimental resolution) with sigma in the isolated state measured recently by X-ray small-angle scattering. The refined shape of isolated sigma was reduced to an ellipsoid which was orientated with respect to the core structure (alpha 2--beta--beta') in a 'space-filling' way around the position of the sigma centre obtained by triangulation. The complete subunit arrangement of holoenzyme is shown in a three-dimensional model.

DNA-Directed RNA Polymerases↗
PubMed1980

[Experimental study on the "traumatogenic" eosinophilic granuloma of the tongue].

According to Bhaskar and Lilly, experimental results with rat tongues indicated that trauma is the cause of the rare eosinophilic granuloma of the human tongue. On the basis of similar experimental investigations, we consider this conclusion to be incorrect. In our opinion, the etiology of eosinophilic granuloma of the tongue as that of other eosinophilic granulomas in man is still unknown.

Animals↗
PubMed1980

[Primary malignant lymphoma of the central nervous system].

Clinical and morphological observation of 56-year-old man with a primary isolated malignant non-Hodgkin's lymphoma of the central nervous system in the hypothalamus, with the formation of metastases in the space of the cerebrospinal fluid and a solitary extraneural metastasis in the epicardium. No generalised lymphoma. The histological classification is discussed. The importance of the cytology of the cerebrospinal fluid to a climical diagnosis is pointed out.

Brain Neoplasms↗
PubMed1979

[Experimental studies on root canal fillings with addition of corticoids].

No clinical symptoms are usually produced by dental procedures utilizing endodontic medicines with corticoid additives. Root fillin pastes with corticoid are particularly deserving of attention. Whether the effect of such root filling pastes is limited to the apical tissue or whether endocrine reactions are also evoked was studied in animal experiments. The possibility of endocrine reactions cannot be definitely excluded. Given the possibility of such counter effects and their sequelae, the discussion of the indications and/or the counterindications for the use of root filling paste with corticoid additives for permanent treatment of root canals should in no way be considered concluded.

Adrenal Cortex Hormones↗
PubMed1977

[The present position of cleft surgery (cheilognathopalatoschisis) (author's transl)].

The present state of cleft surgery enables optimal results to be obtained through operation appropriate to the tissue and development and a longterm treatment lasting until the end of growth. The great individual variation in the clefts demands from the operator exact knowledge of the atypical tissue formations and mastery of the most varied plastic surgical possibilities.

Adolescent↗