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Biomedical subjects

W Hofmann

Publications and source records attributed to W Hofmann.

At least 343 records · Page 19Linked to original sources
PubMed1976

[Myocarditis and insulitis following coxsackie virus infection].

We report on the case of a 5 7/12-year-old girl, who presented an acute insulin-dependent diabetes mellitus nineteen days after the operative suture of an atrial septum defect, and who died in a hyperosmolar coma diabeticum in spite of an intensive therapy. At the autopsy there was shown a myocarditis with the histological characteristics of a virus infection. The islet tissue of the pancreas was largely necrotic, the great majority of the islets of Langerhans presented lymphocytic infiltrates. In a neutralisation test against coxsackie-B-viruses (CBV), which was conducted after death, there was found among others an antibody titer of 1:192 against CBV-4. With FITC-marked CBV-antiglobulin, in the islet tissue intracellular CBV-antigen could be proved. It is discussed that virus infections have an etiological importance for the incidence of a so-called juvenile diabetes in man.

Autopsy↗
PubMed1976

[Experimental kidney preservation for 30 hours in Collins' solution: angiographical and isotope nephrographical findings after autotransplantation].

In experiment canine kidneys preserved in Collins' solution can be kept vital for 30 hours. Angiography after autotransplantation of these kidneys shows good circulation during the immediate postoperative period. Isotope nephrography reveals reversible damage of tubuli which disappears till the 28th day after operation. This procedure of preservation causes tubular damage but does not impair the parenchymatous blood supply. Tubular regeneration therefore is possible.

Angiography↗
PubMed1976

[Aldosteronism].

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Diuretics↗
PubMed1975

[The effect of acetylsalicylic acid, extremely restricted movement and a cholesterol-rich diet on atheromatosis of the rabbit aorta: comparative investigations (author's transl)].

A multifactorial model for demonstrating the pathogenesis of the diet-induced atheromatosis of the rabbit is described. We examined the effect of various diets (atherogenetic, rich in fibre, mixed, normal) and of extreme restriction of movement, with and without doses of acetylsalicylic acid. The aorta showed uniforms morphological findings; 1. Acetylsalicilic acid no influence on the cholesterol-induced atheromatosis of the rabbit; 2. An atherogenic diet and a diet rich in raw fibre caused different degrees of sclerosis of the aorta; this was related to the cholesterol content of the mixed diet, which was 50% less than the cholesterol content of the atherogenic diet; 3. Macro- and microscopic examination showed that extreme restriction of movement alone has no demonstrable effect on the aorta of the rabbit; 4. The cholesterol-induced atheromatosis showed significantly less involvement of the aorta when there was extreme restriction of movement in addition to the diet; 5. In none of the test groups could we demonstrate any effect of PAT I on platelet adhesiveness; 6. The test conditions did not result in an activation of the contact phase of the haemocoagulation system.

Animals↗
PubMed1975

Activity, isoenzyme pattern, and synthesis of UDPglucose 4-epimerase during differtiation of Physarium polycephalum.

1. The specific activity of UDPglucose 4-epimerase (EC 5.1.3.2) increases by about 50% during the first 24 h of starvation-induced differentiation (spherulation) of Physarum polycephalum. 2. At all stages during differentiation, the enzyme activity is very sensitive to actinomycin-C and cycloheximide, inhibitors of transcription and translation, with a half life against cycloheximide of about 20 min (if added 12 h after the induction of differentiation). 3. The isoenzyme pattern, as revealed by isoelectric focusing in sucrose gradients, does not change during spherulation. One main band with a pI of 6.7, with a shoulder (pI 7.6) and a minor band (pI 6.0) was observed in extracts both from growing and differentiating cultures. 4. Density labelling experiments using deuterated amino acids with subsequent analysis by equilibrium density gradient sedimentation in 15-35% (w/w) metrizamide gradients revealed a rather slow rate of enzyme synthesis, which is in contrast to the observed high sensitivity against actinomycin-C and cycloheximide.

Carbohydrate Epimerases↗
PubMed1975

The reaction of the cornea in vivo and in vitro to thermal stimulation: a contribution to the thesis by Busse-Grawitz.

In a test series with 15 rabbits a so-called central keratitis by means of a redhot needle was set in the left and right eye. The cornea of the left eye was immediately extirpated under sterile conditions and was observed in the tissue culture for 6 days. The corresponding right eye of the first rabbit was left within the animal's body, later it was extirpated after 1/2, 1, 1 1/2, 2 hours etc. up to 11 days histologically examined. In the explants following results were obtained. After the lesion is set the puncture site is devoid of epithelium. A double wall of cells lies closely around the crater. One-and-one-half hours later these large cells with more or less variably segmented nuclei demonstrate a strong proliferation. The characteristic nucleus form, the weakly positive naphthol-ASD-chloracetateesterase-reaction and the variable peroxydase-reaction of these cells resemble quite closely leucocytes in phenomenology and ferment-histochemistry. We have therefore chosen the description leucocytoid cells. They are not leucocytes, but cells which originate from basal pluripotent epithelia of the cornea. The so-called central keratitis is not an inflammation in the sense of Marchand, it is rather a regenerative process. The thesis of Busse-Grawitz, that leucocytes in the cornea originate from collagen and elastic fibers or less than coccisized transitional forms, cannot be verified.

Animals↗
PubMed1975

[Mumps myocarditis in a small child].

The clinical history and post-mortem findings in a five-year-old girl are reported. She died in consequence of a chronic mumps myocarditis 13 weeks after having fallen ill with mumps. The antigen specific for mumps virus could be demonstrated in the myocardium by means of immunofluorescence.

Antigens, Viral↗
PubMed1975

[Investigation of living and flattened microorganisms (author's transl)].

In 1973 one of the present authors described a new method of cytological observation based upon flattening of living cells. This method was tested with several microorganisms as bacteria, fungi and algae. The application of the method allows the clear resolution of cytological structures that are otherwise obscured or can hardly be recognized. In addition cellular motion and development can be followed much better than by usual methods of cell culture. In certain objects (yeasts and fungal hyphae tips) all cellular organelles are forced into one plane of focus and are thus resolved simultaneously even at highest magnification. Different species especially among the yeasts exhibited quite varying intracellular contrast of mitochondria.

Amoeba↗