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Biomedical subjects

W Grote

Publications and source records attributed to W Grote.

At least 73 records · Page 4Linked to original sources
PubMed1990

Inv(14)(q11q32) in one of four different clones in a case of angioimmunoblastic lymphadenopathy.

A case of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) with four cytogenetically different cell clones (49,XX,+5,+19,+21/47,XX,+X/46,XX,inv (14)(q11q32)/45,X,-X) is reported. To our knowledge, this is the first case of AILD with an inv(14)(q11q32), thus probably involving the T-cell receptor alpha-chain gene. The cytogenetic findings are discussed with respect to the possible progression of AILD to malignant lymphoma.

Chromosome Banding↗
PubMed1990

[Complications of ventral fusion operations in degenerative intervertebral disk diseases].

1112 anterior cervical fusion operations were performed on 951 patients according to the method described for the first time by Grote in 1970. The neurogenic, mechanical and inflammatory complications arising with this method are described. The typical complications are demonstrated by means of case reports and their possible causes are discussed. The incidence rate of the individual complications is compared with that reported in the literature for a total of 8366 fusions performed on 6384 patients.

Cervical Vertebrae↗
PubMed1990

Mycoplasma fermentans-derived high-molecular-weight material induces interleukin-6 release in cultures of murine macrophages and human monocytes.

A Mycoplasma fermentans-derived high-molecular-weight material (MDHM) is described which causes differentiation of concanavalin A-stimulated CBA/J or C57BL/6 mouse thymocytes to cytolytic effector T cells (CTLs). The effect of MDHM was inhibited by addition of monoclonal anti-interleukin-6 (IL-6) antibody. It could also be abolished after removal of adherent cells. However, adherent cell-depleted thymocytes could still form CTLs after addition of IL-6. The action of MDHM could thus be explained by the capacity of MDHM to stimulate IL-6 release from adherent cells. MDHM was active on macrophages from CBA/J and C3H/HeJ endotoxin nonresponder mice and was also capable of stimulating IL-6 release from human monocytes. On gel chromatography, MDHM had an apparent molecular size of 1.5 x 10(6) daltons. Treatment with RNase and DNase had no effect on either size or biological activity. Proteinase K did not abolish activity but reduced the apparent molecular size of MDHM. MDHM production by M. fermentans required either coculture with eucaryotic cell lines in RPMI 1640 medium with fetal calf serum or addition of eucaryotic cell sonic extracts to this medium. The biological activity of MDHM is not identical to that of a mitogen for murine spleen cells derived from M. arthritidis; MDHM caused only slight proliferation in this system compared with the mitogen from M. arthritidis, and the latter did not elicit IL-6 release from macrophages. The results are discussed in relation to mycoplasmas as putative etiological agents for rheumatoid arthritis, since high IL-6 titers were reported for synovial fluid from patients with this disease.

Animals↗
PubMed1990

Angioimmunoblastic lymphadenopathy with trisomy 3: the cells of the malignant clone are T cells.

A method involving simultaneous demonstration of the immunophenotype and the karyotype was applied to a case of angioimmunoblastic lymphadenopathy (AILD), in which the standard chromosome preparation revealed an aberrant clone with trisomy 3 and additional changes. The aberrant mitoses showed a positive reaction with the monoclonal T-cell antibody Leu 4 (CD3). This new method thus provides definite evidence that the malignant clone with trisomy 3, a characteristic finding in AILD, belongs to the T-cell population. Nevertheless, cytogenetically normal T cells were also found. Moreover, a cell from the same clone was found in the phytohemagglutinin-stimulated blood lymphocytes. This finding demonstrates that AILD may have a leukemic variant.

Chromosomes, Human, Pair 3↗
PubMed1990

[Hypertelorism-hypospadias (BBB) syndrome. 2 additional family studies].

We report on two unrelated families with the hypertelorism-hypospadias (BBB-) syndrome. The male index patients, 3 and 10 months old, respectively, have ocular hypertelorism, cleft lip and palate, high and broad nasal bridge and hypospadias. The patients' mothers, maternal grandmothers and one patient's sister show hypertelorism. In addition, we summarize the characteristics of previously published cases.

Bone Diseases, Developmental↗
PubMed1990

[Intracranial complications of inflammatory ear diseases].

Acute and chronic ear infection can lead to intracranial complications, brain abscess and meningitis being the most serious. The clinical picture, diagnosis and therapeutic guidelines are discussed. The important role of interdisciplinary management is emphasised by a case report.

Adult↗
PubMed1989

Common fragile sites in couples with recurrent spontaneous abortions.

Recently, increased spontaneous chromosome instability was reported in couples with recurrent spontaneous abortions but without constitutional chromosome aberrations. Therefore, we investigated the frequency and distribution of aphidicolin-induced common fragile sites in couples with recurrent spontaneous abortions and no children and in age-related control couples. The breakage rate was significantly elevated in the abortion-prone couples; the women in the abortion couples had an especially higher breakage rate than the control women. Breakpoints cluster to those chromosome regions where common fragile sites have been localized. No preference of a particular common fragile site was demonstrated in the abortion couples. Our findings appear to support the hypothesis of increased chromosome instability in at least some couples with recurrent spontaneous abortions. As long as the nature of aphidicolin-induced common fragile sites is not completely understood, the significance of these findings remains unclear.

Abortion, Habitual↗
PubMed1989

Clinical outcome after head injury in children.

For at least 1 year we have done a follow-up on 178 children under the age of 18 years with head injuries treated between 1981 and 1987. The Brussel Coma Scale and the Glasgow Outcome Score were used to determine neurological deficits and patient outcome. In children with coma, there was good recovery or moderate disability in 71.4%, severe disability in 6.1%, and death in 22.5%. When all head injuries were taken into consideration, a satisfactory outcome was found in 84.3%, severe disability in 3.3%, and death in 12.4%. The outcome mainly depended on the initial coma grade, but the duration of coma or advanced age were also negative factors for outcome. In 14.8%, intracranial mass lesions showed no significant correlation with outcome. In contrast to adults, diffuse brain swelling had a satisfactory outcome in 85.1%.

Adolescent↗
PubMed1989

Spinal metastasis of pilocytic astrocytoma of the chiasma opticum.

We report a case of spinal seeding of a pilocytic astrocytoma of the chiasma opticum. Microscopic examination of the optic nerve tumor and the spinal tumor showed the same pathological patterns, without any signs of anaplastic transformation. The clinical course demonstrates the low growth rate of this kind of tumor, but spinal metastasis has not yet been described. We discuss different pathophysiological explanations of this atypical biological behavior with reference to the literature.

Astrocytoma↗
PubMed1989

Sellar chondroma in a case of Ollier's disease.

Chondromas are rare intracranial tumours. Sometimes they occur as a manifestation of a 'generalised chondromatosis'. The authors present a case of sellar chondroma in a patient with Ollier's disease. The tumour showed a mainly suprasellar extension resulting in a chiasmatic syndrome. The pathogenesis of intracranial chondromas, their clinical features, and CT- and MRI-findings are discussed.

Adult↗
PubMed1989

[Syringomyelia from the neurosurgical viewpoint].

Indications for, and methods of, surgical treatment in patients with syringomyelia are described. In 16 patients a syringomyelic cavity was drained. Clinical, radiological and operative findings and results are reported and discussed. In six of twelve patients followed up, progressive deterioration was arrested by operation and two patients improved.

Adolescent↗
PubMed1988

Non-invasive follow-up of patients with intracranial arteriovenous malformations after proton-beam radiation therapy.

In order better to follow non-invasively the effect of proton beam therapy upon intracranial arteriovenous malformations, transcranial Doppler sonography (TCD) was used in 10 patients, in addition to conventional follow-up with angiography and computed tomography (CT); in six patients, pre- and postradiation TCD studies were obtained. The mean flow values obtained from the major arteries in the circle of Willis indicate an early flow reduction through the AVM of approximately 15% with further reduction between 2/3 and 1/2 of the initial values within 15 months of follow-up. CT showed a reduction of contrast enhancement in those patients in whom scans could be compared. In this limited experience, TCD was proven to yield additional information, in repeated non-invasive studies, on the haemodynamic influence of AVMs and the effect of proton beam therapy.

Cerebral Angiography↗
PubMed1988

Choice of surgical approach in orbital space occupations--an interdisciplinary problem.

During the last two and a half years 60 patients with orbital space occupying neoplasms were treated surgically at the Department of Maxillo-facial Surgery of the University Clinic of Essen. With modern imaging techniques exact information on the location and extent of space occupation was available before the operation. Therefore the surgical approach could be optimized. Despite the various and sometimes inaccessible tumor locations, transcranial operation was only necessary in six patients. In the remaining 54 patients anterior orbitotomy allowed either a definitive tumor therapy or histological verification of the diagnosis.

Hemangioma↗
PubMed1988

[Indications and results of sensory rhizotomy in cicatricial lumbar radiculitis syndromes].

12 out of 2076 patients (0.6%) with lumbar disc surgery from 1980 to 1986 were selected for dorsal rhizotomy because of persisting complaints. Almost all of these patients had several lumbar operations before undergoing rhizotomy. The long-term results are very poor. Only 15.4% of all patients showed pain relief, 70% did not return to their employment. Complications occurred in 23%. Patient selection modalities for operation and pathophysiologic causes explaining the bad results are discussed.

Adult↗
PubMed1987

Large scale production and purification of human IL-2 from buffy coat lymphocytes stimulated with 12-O-tetradecanoylphorbol 13-acetate and calcium ionophore A23187.

Methods for the production of high titers of interleukin-2 (IL-2) from human buffy coat lymphocytes, and subsequent purification of the IL-2 are described. 50 buffy coats containing 1 X 10(11) leukocytes were first depleted of erythrocytes by batchwise leukapheresis using a Haemonetics model 15 blood wash centrifuge. Further lymphocyte enrichment was achieved using a one-step sedimentation in the presence of hydroxyethyl starch, which produced suspensions of more than 90% lymphocytes. This degree of lymphocyte purity was important since phagocytes were inhibitory to 12-O-tetradecanoylphorbol 13-acetate/calcium ionophore (TPA/A23187)-induced IL-2 production when their concentration exceeded 15% of the total cells. Cell culture was performed in stirred fermenters. Using TPA/A23187 induction, up to 500 micrograms of IL-2 per liter were produced. The IL-2 was purified by absorption from the supernatants onto controlled pore glass and elution with 50% ethylene glycol, followed by Fractogel chromatography, and then preparative high-performance liquid chromatography (HPLC) using an RP-6 column and elution with a gradient of n-propanol. A final HPLC rechromatography step using an analytical RP-6 column gave a homogeneous preparation with specific activity of 1.2 X 10(7) U/mg and a recovery from the starting supernatant of 22%.

Calcimycin↗
PubMed1987

Intralymphatic interleukin-2 treatment of a hemophiliac AIDS patient with defective interleukin-2 production.

To improve immune functions in an interleukin-2 (IL-2) deficient hemophiliac AIDS patient suffering from severe Pneumocystis carinii pneumonia, treatment with IL-2 was started in addition to standard antimicrobial therapy. Highly purified IL-2 was administered subcutaneously and then repeatedly intralymphatically in a manner similar to pedal lymphography. No toxicity was observed. The patient temporarily improved clinically as well as with regard to immunological functions. Particularly the in vitro response to phytohemagglutinin (PHA) could partly be restored, and skin tests revealed improved response to recall antigens. These findings indicate that IL-2 can be administered safely and effectively by the intralymphatic route and may--in addition to antibiotics--be of value in AIDS patients with severe opportunistic infections.

Acquired Immunodeficiency Syndrome↗