Biomedical subjects
W C Lambert
Publications and source records attributed to W C Lambert.
Inflammatory metastatic carcinoma of the parotid.
Explore the source record for details and available documents.
Oral warty dyskeratoma.
Warty dyskeratoma of the oral mucosa is a rare cause of solitary mouth lesions. Of the thirteen reported patients, only three have been described in the dermatologic literature. We now present an additional patient, and review both the medical and the dental literature on warty dyskeratoma. Various factors possibly related to its cause are discussed, including cigarette smoking, a heretofore largely unappreciated possibility.
Nuclear DNA endonuclease activities on partially apurinic/apyrimidinic DNA in normal human and xeroderma pigmentosum lymphoblastoid and mouse melanoma cells.
DNA endonuclease activities from nuclear proteins of normal human and xeroderma pigmentosum (XP), complementation group A, lymphoblastoid and Cloudman mouse melanoma cells were examined against partially apurinic/apyrimidinic (AP) DNA. Non-histone chromatin-associated and nucleoplasmic proteins, obtained from isolated nuclei, were subfractionated by isoelectric focusing and assayed for DNA endonuclease activity against linear, calf thymus DNA. All of the nine chromatin-associated and three of the nucleoplasmic fractions, which lacked DNA exonuclease activity, were tested for DNA endonuclease activity against both native and partially AP, circular, duplex, supercoiled PM2 DNA. In all three cell lines, four chromatin-associated, but none of the nucleoplasmic fractions, showed increased activity against DNA rendered AP by either heat/acid treatment or by alkylation with methyl methanesulfonate (MMS) followed by heat. One chromatin-associated activity, with pI 9.8, which was not active on native DNA, showed the greatest activity on AP DNA. AP activity was moderately decreased in XP cells and slightly decreased in mouse melanoma cells, as compared with normal cells, in the fraction at pI 9.8. Little or no increased activity was observed in any of the endonucleases from any of the cell lines on MMS alkylated DNA.
Acrokeratosis paraneoplastica (Bazex' syndrome). Report of a case and review of the literature.
Acrokeratosis paraneoplastica (Bazex' syndrome) is a rare but clinically distinctive dermatosis that has been associated in all reported cases, to our knowledge, with either a primary malignant neoplasm of the upper aerodigestive tract or metastatic cancer to the lymph nodes of the neck. Acrokeratosis paraneoplastica was found in a 53-year-old black man with squamous cell carcinoma of the tonsil. A distinctive series of changes was found on histopathologic examination of biopsy specimens taken from his skin lesions, and direct immunofluorescence microscopy of both lesional and nonlesional skin specimens showed immunoglobulin and complement deposition on the epidermal basement membrane. The skin lesions largely resolved following radiation therapy of the neoplasm and of the presumably involved lymph nodes.
Increased sensitivity of a xeroderma pigmentosum lymphoblastoid cell line to serum deprivation in vitro.
Two human lymphoblastoid cell lines, GM 1989, from a normal individual, and GM 2345, from a patient with xeroderma pigmentosum, complementation group A, were selected for comparative biochemical studies because they both grow rapidly and at virtually identical rates in sealed flasks in RPMI 1640 medium buffered to physiological pH with HEPES buffer, supplemented with 12% heat-inactivated fetal bovine serum. Although the two cell lines showed no difference in growth parameters assayed by standard methods, further studies showed that the GM 2345 cell line was markedly more sensitive to diminution of the serum concentration of the culture medium than was the normal cell line. These results indicate that lymphoblastoid cell lines, particularly those from individuals with certain genetic or metabolic diseases, may be growing under marginal or limiting circumstances, different from those of control cell lines, which are not detected by standard techniques used to monitor mammalian cell cultures.
Culture of Vibrio extorquens from severe, chronic skin ulcers in a Puerto Rican woman.
A 48-year-old Puerto Rican woman developed extensive ulcers on her buttocks, right arm, and thighs over a 3 1/2-year period. The lesions began as small, subcutaneous nodules which subsequently ulcerated and expanded up to 19 cm in diameter. Biopsy of both ulcerated and nonulcerated lesions showed acid-fast bacilli. Culture of both types of lesions grew Vibrio extorquens, a partially acid-fast methanolophilic organism not previously associated with disease in humans. The patient developed agglutinating antibody, titer 1:80, to this organism. The disease responded to treatment with antibiotics to which the organism was sensitive in vitro.
Diagnostic pitfalls in anhidrotic ectodermal dysplasia: indications for palmar skin biopsy.
Explore the source record for details and available documents.
Nuclear deoxyribonuclease activities in human lymphoblastoid and mouse melanoma cells. A comparative study.
Deoxyribonuclease activities were examined in isoelectric focusing fractions of non-histone, chromatin-associated and nucleoplasmic proteins of isolated normal human lymphoblastoid and mouse melanoma cell nuclei using parallel procedures. A very similar series of eight DNA endonucleases, each active on calf thymus DNA and containing no exonuclease activity, were found in the chromatin proteins of both cell lines. Several differences were observed: an activity in human cells at pI 6.6 was absent from murine cells, and there was an increased activity in mouse cells at pI 4.4 and a decreased activity at pI 7.3, as compared with corresponding human cell activities. Assay of these fractions against supercoiled, circular phage PM2 DNA showed greater activity among the fractions with acidic pI valves and slightly lower activities in the murine cells than in the human cells. Analysis of the nucleoplasmic fractions showed a series of DNA endonuclease and exonuclease activities which were again very similar between the two cell lines, although greater endonuclease activity at pI 4.4 occurred in mouse than in human nucleoplasm. These results demonstrate an entire series of deoxyribonuclease activities in both chromatin and nucleoplasm which are nearly identical in two very different mammalian cell lines, suggesting that many of these enzymes are ubiquitous in mammalian cell nuclei.
Nuclear deoxyribonuclease activities in normal and xeroderma pigmentosum lymphoblastoid cells.
Explore the source record for details and available documents.
Defective DNA endonuclease activity on anthramycin treated DNA in xeroderma pigmentosum and mouse melanoma cells.
Explore the source record for details and available documents.
Leukocytoclastic angiitis induced by clindamycin.
A fifty-six-year-old white male diabetic developed leukocytoclastic angiitis after treatment with clindamycin for an infected foot ulcer. The eruption subsided following withdrawal of the drug, recurred when it was re-administered, and cleared after it was again withdrawn. Clindamycin or lincomycin allergy should be considered in the differential diagnosis of leukocytoclastic angiitis in patients who take these drugs.
The nosology of parapsoriasis.
Parapsoriasis is a group of uncommon but not rare disorders that was created in 1902 as part of a now long forgotten scheme to classify all inflammatory dermatoses. This artificial grouping has led to an enormously confused nosology of these disorders, that are, for the most part, otherwise unrelated. The use of a number of different terms at different institutions and by different physicians to denote the same diseases, together with the use of particular, single terms to denote different diseases, has caused much unnecessary confusion. In this review these terms are examined and an attempt is made to propose a new, unambiguous classification. Using this system, physicians with different views regarding which of the parapsoriases constitute distinct entities should have not difficulty communicating, and should have clearer frame of reference within which to work. An attempt is made to evaluate critically which of the parapsoriases are distinct entities and whether one or more of them should be considered an early form of cutaneous lymphoma in light of currently available data.
The cyclic, six-carbon polychlorides: frequently confused compounds with markedly different applications and toxicities.
Explore the source record for details and available documents.
Letter: Characteristics of the antibody response of rabbit spleen cells in vitro.
Explore the source record for details and available documents.
Origin of antibody-dependent, lymphoid-cell-independent hemolytic plaques in rabbit spleen cell cultures.
This report describes a phenomenon that may be a significant source of error in the data acquired from hemolytic plaque assays of lymphocyte cultures. The data show that background antibody in the normal-serum supplement of the culture medium does not actually support initiation of a vigorous primary-type plaque-forming cell (PFC) response to sheep erythrocytes (SE). Instead, the background antibody causes the development of numerous spurious lymphoid-cell-independent plaques (LCIP). In general agreement with the literature, primary-type rabbit spleen cell responses in vitro were low in culture media supplemented with fetal bovine serum, a serum which does not contain background antibodies to SE.
Thymidine as a synchronizing agent. I. Nucleic acid and protein formation in synchronous HeLa cultures treated with excess thymidine.
Explore the source record for details and available documents.
Thymidine as a synchronizing agent. II. Partial recovery of HeLa cells from unbalanced growth.
Explore the source record for details and available documents.