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Biomedical subjects

W Bender

Publications and source records attributed to W Bender.

At least 109 records · Page 6Linked to original sources
PubMed1983

Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.

A chromosomal walk is described that covers 315 X 10(3) base-pairs of DNA from the 87DE region of the third chromosome of Drosophila melanogaster. The walk includes the DNA for the rosy and Ace loci, which code for xanthine dehydrogenase and acetylcholinesterase, respectively. Several dispersed repetitive elements were encountered in the walk. In every case, their positions in the chromosome differed in different strains, and so they are all presumed to be transposable elements. Several rearrangement breakpoints have been localized within the walk, including the break for In(3R) Cbx+R1 (87E1, 2-89E1, 2). One breakpoint fusion fragment of this inversion was isolated to jump from 87E into the cluster of homeotic genes of the bithorax complex, at 89E1-4.

Acetylcholinesterase↗
PubMed1983

Molecular mapping of genetic and chromomeric units in Drosophila melanogaster.

We have used a set of overlapping cloned segments defining a 315 kb (X 10(3) base-pairs) region of Drosophila melanogaster chromosomal DNA to map the sequences associated with the polytene band-interbands (chromomeric units) and with the lethal complementation groups contained within this region. The molecular map positions of the 13 +/- 1 chromomeric units from the 87D5-6 to 87E5, 6 region of the third chromosome were determined by in situ hybridization of selected segments to the polytene chromosomes. The length of the largest chromomeric unit within the 315 kb region is approximately 160 kb, while that for the smallest is less than 7 kb and may be as short as 3 kb. By mapping the breakpoints of deletions within the 315 kb region, we have located its 12 lethal complementation groups, which include the genes coding for acetylcholinesterase (Ace) and xanthine dehydrogenase (rosy). Comparison of the two molecular maps indicates a one-to-one topographical correlation between the genetic and chromomeric units.

Animals↗
PubMed1983

Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.

Certain spontaneous mutations of Drosophila melanogaster are suppressed by su(Hw), the suppressor of Hairy-wing (3R-54.8). We find that mutations suppressible by su(Hw) result from insertions of a mobile element at the affected loci. The element, named gypsy, is approximately 7.3 kilobases long and includes 0.5-kilobase direct terminal repeats. It was first identified in DNA cloned from the bithorax chromosomal region of several Drosophila stocks carrying suppressible mutations of the bithorax complex. Cloned gypsy DNA was used as a probe to test for the association of gypsy with suppressible mutations at various other loci by hybridization in situ. Gypsy was found to be associated with 19 suppressible alleles at 10 different loci: yellow, Hairy-wing, scute, diminutive, cut, lozenge, forked, Beadex, hairy, and the bithorax complex. It was found with wild-type or nonsuppressible mutations at any of these loci. Gypsy DNA was also used as a probe to clone the element and adjacent unique DNA from the loci of some suppressible mutations. This confirmed the presence of the full-length element and also provided cloned DNA from the previously uncloned loci scute and cut. The suppressor of Hairy-wing is generally recessive and behaves as a null mutation. Thus, the disruption of normal gene function caused by the inserted gypsy element appears to require some product of the wild-type suppressor gene, su(Hw)+.

Animals↗
PubMed1983

[Psychiatric consultation service in a large medical clinic: evaluation over a 3-year period].

The report is based on 1,165 medical inpatients seen by the consultant psychiatrist within a 3-year period. During the last year evaluated for this study psychiatric consultation was requested for 2.8% of the total non-psychiatric hospital admissions. Questions to the consultant are most often referred to psychogenic factors, depression and suicidality. The most frequent psychiatric diagnosis was neuroses/reactions/ personality disorders followed by suicidality, organic psychoses, psychosomatic illnesses, endogenous psychoses, somatopsychic syndromes and addictions. Further details and problems in psychiatric consultation are discussed.

Depressive Disorder↗
PubMed1980

[Comparison of nondominant unilateral and bilateral electroconvulsive therapy--clinical efficiency and side effects (author's transl)].

UNLABELLED: In a double-blind study 100 patients were treated either with conventional ECT or with unilateral ECT. The majority suffered from depressive illness, mainly endogenous depression. On average, they were hospitalized 41 days and unsuccessfully treated with antidepressants and other psychoactive drugs before ECT was given as a last treatment resort. ECT efficiency was evaluated by: (1) clinical evaluation, (2) AMP documentation, (3) the Hamilton Rating Scale for Depression, and (4) the von Zerssen Mood Scale. Side effects were evaluated by a test (1) for vigilance (tachistoscopic threshold), (2) for verbal and visual memory, (3) for performance, (4) for concept formation, and (5) the Benton test. RESULTS: There was no difference in clinical efficiency between the two methods. As for side effects, unilateral ECT produced fewer organic brain syndromes and less impairment of verbal memory and performance. Total side effects were significantly less. There was no significant difference between the two methods with regard to vigilance, visual memory, and concept formation. One week after ECT, in both groups there was a significant increase in vigilance and visual memory in comparison to the results before ECT; the unilateral group also showed significant increase in verbal memory and concentration.

Adult↗
PubMed1980

[Therapeutic stereotypes among the mentally ill].

Using mathematical models described by Overall, the authors investigated in a sample of 301 psychiatric inpatients the therapeutic stereotypes in medicating psychotropic drugs and its dependence on symptom, syndrome and diagnostic level. The psychotropic drugs were described in a dimensional representation of psychopathology ('symptomatic space'). Besides dependencey from patient's characteristics as age and clinician's characteristics as special orientation, the therapeutic stereotype is mostly influenced by the symptom level. Only lithium therapy and electroconvulsive therapy are keyed to diagnosis. Getting more familiar with a psychotropic drug increases indications and contraindications on symptom level, the therapeutic stereotype gets more differentiated. The comparison between the therapeutic stereotypes of German and French psychiatrists shows a satisfactory congruence relating to dimensionality and relationships among the drugs.

Adult↗
PubMed1980

[Drug abuse].

Explore the source record for details and available documents.

Germany, West↗
PubMed1979

[Sensitivity of creatine kinase and creatine kinase MB in myocardial infarction. Evaluation of a new optimized standard method (author's transl)].

In 70 patients with confirmed transmural myocardial infarction the sensitivity of creatine kinase and creatine kinase MB at different times after admission was investigated by 364 measurements. Beside the number of correctly positive results the optimized standard method hitherto used was compared with the new optimized standard method with N-acetylcystein as activator. During the first 12 hours after admission the percentage of correctly positive results was 64,4% for creatine kinase (GSH) and 86,7% for creatine kinase (NAC). The sensitivity of cretine kinase MB, however, was found to be 71,1% (GSH) and 86,7% (NAC). With respect to the poor specifity of creatine kinase the sensitivity of creatine kinase MB, especially when using the new optimized standard method is superior. Similar results were established 24 and 48 hours after admission. Creatine kinase MB only fails in detection of myocardial infarction if the stroke is older than 3 days or if the enzyme activity determination intervals are too long.

Acetylcysteine↗