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W Barendse

Publications and source records attributed to W Barendse.

At least 91 records · Page 5Linked to original sources
PubMed1993

Physically mapped, cosmid-derived microsatellite markers as anchor loci on bovine chromosomes.

To identify physical and genetic anchor loci on bovine chromosomes, 13 cosmids, obtained after the screening of partial bovine cosmid libraries with the (CA)n microsatellite motif, were mapped by fluorescence in situ hybridization (FISH). Eleven cosmid probes yielded a specific signal on one of the bovine chromosomes and identified the following loci: D5S2, D5S3, D6S3, D8S1, D11S5, D13S1, D16S5, D17S2, D19S2, D19S3, D21S8. Two cosmids produced centromeric signals on many chromosomes. The microsatellite-containing regions were subcloned and sequenced. The sequence information revealed that the two centromeric cosmids were derived from bovine satellites 1.723 and 1.709, respectively. A cosmid located in the subtelomeric region of Chromosome (Chr) 17 (D17S2) had features of a chromosome-specific satellite. Primers were designed for eight of the nonsatellite cosmids, and seven of these microsatellites were polymorphic with between three and eight alleles on a set of outbred reference families. The polymorphic and chromosomally mapped loci can now be used to physically anchor other bovine polymorphic markers by linkage analysis. The microsatellite primers were also applied to DNA samples of a previously characterized panel of somatic hybrid cell lines, allowing the assignment of seven microsatellite loci to defined syntenic groups. These assignments confirmed earlier mapping results, revealed a probable case of false synteny, and placed two formerly unassigned syntenic groups on specific chromosomes.

Animals↗
PubMed1993

A genetic map of index DNA loci on bovine chromosome 21.

Genetic maps of highly polymorphic index DNA loci exist essentially only in humans and some experimental organisms. Here we report the first genetic map of highly polymorphic index DNA loci in livestock for bovine chromosome 21. This map consists of six loci with an average heterozygosity of 82%, each with a minimum of five alleles, spaced at an average genetic distance of 9.7 cM, and covers most of the expected length of the acrocentric bovine chromosome 21. The order of markers along the chromosome is cen-ETH 131-UWCA 4-TGLA 337-TGLA 122-CSSM 18-GMBT 16-tel. There is heterogeneity among the recombination fractions between the sexes.

Animals↗
PubMed1993

A genetic map of DNA loci on bovine chromosome 1.

We constructed a genetic map of most of the length of bovine chromosome 1 using the CSIRO and the Texas A&M University cattle reference families. Twelve loci are in a single linkage group, 9 of which are highly polymorphic loci. Four loci are of known biochemical function, alpha-1 crystallin (CRYA1), gamma-s crystallin (CRYG8), superoxide dismutase 1 (SOD1), and uridine monophosphate synthase (UMPS), and these have also been previously mapped in humans. The loci CRYA 1, CSRD 1613, GMBT 7, RM 95, SOD1, and UMPS had been previously assigned to bovine syntenic group U10, while CSRD 1613 and UMPS had also been assigned to chromosome 1 by in situ hybridization. All of the loci show statistically significant linkage to at least one other locus. The conserved loci indicate that there have been major rearrangements during the evolution of bovine chromosome 1 compared to other mammalian chromosomes. The estimate of the total length of the linkage group is 168 cM, which accords well with the predicted length based on chiasmata frequencies for the bovine genome and the relative size of chromosome 1 in the bovine genome.

Animals↗
PubMed1993

The gamma fibrinogen gene (FGG) maps to chromosome 17 in both cattle and sheep.

The gamma fibrinogen gene (FGG) was localised in both cattle and sheep using in situ hybridisation. The probe employed was a 1-kb bovine cDNA fragment. Based on observations of QFQ-banded chromosome preparations, this locus is on bovine chromosome 17q12-->q13 and on the homologous sheep chromosome 17. This localisation is, to our knowledge, the first assignment to chromosome 17 in either the bovine or ovine genome. In addition to localising FGG to this chromosome, the assignment provisionally maps the previously unassigned syntenic group U23, containing (besides FGG) the genes for mitochondrial aldehyde dehydrogenase 2 (ALDH2), interleukin 2 (IL2), immunoglobulin lambda (IGL), and beta fibrinogen (FGB), to chromosome 17 in cattle and probably to the same chromosome in sheep.

Animals↗
PubMed1992

Linkage relations between A2M, HOX3, INT1, KRAS2, and PAH on bovine chromosome 5.

There is a high level of conservation between human chromosomes and bovine syntenic groups. One such comparison is between human chromosome 12 and bovine chromosome 5, where at least 16 loci have been shown to be conserved in an homologous segment. However, the degree of conservation of order of the loci on bovine chromosome 5 is unknown, and in general the conservation of order in comparisons between humans and cattle can only be speculated. We have estimated the recombination fractions between five of the loci that were previously published as mapping to bovine chromosome 5 by a combination of in situ hybridization and analysis of bovine-rodent somatic cell hybrid lines to determine whether order has been conserved in the homologous segment of bovine chromosome 5 and human chromosome 12. Recombination fractions were estimated in reference pedigrees of cattle. The loci were A2M, GSNL, HOX3, INT1, KRAS2, and PAH. Restriction fragment length polymorphisms for all loci were defined by screening a panel of eight restriction endonucleases. The linkage between loci was estimated using the lod score method, and all possible pairwise comparisons were made. A preliminary map was created by joining together loci that showed the smallest recombination fractions and the largest lod scores. A multipoint analysis was performed to estimate support for the most likely order. This order shows the relative inversion of some of the loci. Moreover, the distance spanned in cattle is less than a quarter the distance spanned in humans. Together, these data indicate that several chromosomal evolutionary events have occurred in the homologous segment shared by humans and cattle.

Animals↗
PubMed1992

Bovine and ovine DNA microsatellites from the EMBL and GENBANK databases.

Bovine and ovine microsatellite sequences were extracted from the EMBL and GENBANK databases. When analysed for number of alleles and degree of heterozygosity in the CSIRO cattle reference families, allele numbers range from 1 to 14 with heterozygosities, in the polymorphic systems ranging from 15.8% to 100%. Six (46%) of the 13 bovine systems tested gave specific and polymorphic products in sheep. Similarly 2 of the 4 ovine systems gave specific and polymorphic products in cattle. These data define 11 bovine and 8 ovine microsatellite systems which are associated with known genes and are thus useful for comparative mapping studies.

Alleles↗
PubMed1990

Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in situ hybridization.

The growth hormone gene locus (GH) of cattle and sheep was mapped to a chromosomal region in each species by using in situ hybridization. The probe employed was an 830-bp cDNA sequence from the ovine growth hormone gene. Based on QFQ chromosome preparations, our results show that the GH locus is on cattle chromosome 19 in the region of bands q26-qter and in sheep on chromosome region 11q25-qter. The GH assignments together with previous localizations of type I cytokeratin genes (KRTA) and one homeobox (HOX2) gene in cattle and one type I cytokeratin gene (KRTA) in sheep identify a strongly conserved chromosomal segment on human chromosome 17, bovine chromosome 19, and sheep chromosome 11.

Animals↗