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Biomedical subjects

W Ahne

Publications and source records attributed to W Ahne.

At least 37 records · Page 2Linked to original sources
PubMed1996

Immunoreactive cytokines within primates.

Peripheral blood mononuclear cells of primates (man, orang utan, gorilla, baboon), rodents (mouse, rat), carnivores (cat, dog), artiodactyls (cattle, goat, pig) and perissodactyls (horse) were isolated and stimulated with mitogens (5 micrograms/ml LPS, 5 micrograms/ml PHA) at 37 degrees C. Cytokines immunoreactive to monoclonal antibodies (mAb) directed to human cytokines (TNF-alpha, IL-1 alpha, IL-2, IL-6, IFN-gamma) could be detected by enzyme-linked immunosorbent assay (ELISA) in the case of primates only. The mAb used did not recognize cytokines of the other mammalian species investigated. The results demonstrate the close relationship within the primates from the immunophysiological point of view.

Animals↗
PubMed1996

[Immune response of fishes with respect to phylogenetic aspects].

The immune response progressed by steps in the phylogeny of animals. The non-specific immune response has been improved by specific immune mechanisms during the early evolution of the organisms. Typical B- and T-cells as well as true antibodies originated first in fishes during the phylogenesis. Fishes possess immune mechanisms which appear to be homologous to those of higher vertebrates.

Animals↗
PubMed1995

In vitro replication of a reptilian adenovirus.

The morphological alterations of IgH-2 cells infected with a reptilian adenovirus were investigated during 1 cycle of virus replication. The virus particles entering the cell 1 h post infection (pi) were present in cytoplasmic receptosomes few hours later. About 24 h pi nucleocapsids entered the nucleus. Eosinophilic granulation of the nucleus and intra-nuclear inclusion bodies appeared between 30-60 h pi. Small round and ring-shaped (type I), circular, semicircular or rod-shaped (type II) and large, sharp, round (type III) inclusion bodies were formed. Typical crystalline aggregates of virions appeared at the periphery of the nucleus 48-60 h pi. The release of virions into the cytoplasm was followed by lysis of the infected cells. The destruction of cell monolayer was evident 96 h after infection.

Adenoviridae↗
PubMed1993

Physicochemical properties and cytopathogenicity of an adenovirus-like agent isolated from corn snake (Elaphe guttata).

A virus isolated from the internal organs of a moribund corn snake (Elaphe guttata) replicated in reptilian cell cultures (IgH-2, TH-1 cells) between 10 and 30 degrees C. Highest infectivity titers of 10(5.5) TCID50/ml were obtained in IgH-2 cells at 25 degrees C. Infected IgH-2 cells showed the development of three morphologically different intranuclear inclusion bodies. During viral assembly the particles formed typical crystalline aggregates in the nucleus. About 64 h after infection progressive desintegration of the nuclear membrane was evident and virus particles were released into the cytoplasm. Different fish cell lines (CLC, CHSE-214, BF-2, PG, RTG-2) were not capable of propagating the virus. The DNA containing agent proved to be stable at pH3, more or less at pH 12 and to treatment with chloroform, but it was rapidly inactivated at 56 degrees C. Electron microscopy revealed nonenveloped icosahedral particles with a diameter of 65-70 nm.

Adenoviridae↗
PubMed1993

Viruses of Chelonia.

Viruses occurring in turtles and tortoises are hetergeneous but according to ecologic characteristics and pathogenic properties they can be divided in two major groups: 1. Arboviruses (toga-, flavi-, rhabdo- and bunyaviruses) transmitted by arthropods cause severe diseases in homoiothermic vertebrates. The viruses are of great epidemiological interest in human and veterinary medicine. Chelonia and other reptiles infected by bites of vectors e.g. Aedes, Anopheles, Culex develop cyclic viremia without injury. The ectothermic animals maintain inapparent arbovirus infections during hibernation and they play role as reservoirs for these viruses. 2. Viruses of Chelonia origin (papova-, herpes-, irido- and paramyxoviruses) associated with diseases of infected turtles and tortoises have been described frequently during the last 20 years. Several viruses or virus-like particles could be demonstrated in affected reptiles mainly by electron microscopy. Especially herpesviruses seem to attack Chelonia and epizootics due to infections with these viruses were reported in several reptiles in collections. However, the etiological role of the agents detected is not well documented yet.

Animals↗
PubMed1993

[The scientific nomenclature of domestic animals].

The nomenclature of domestic animals is based on the scientific names of wild ancestors. Bohlken suggested adding the annex "forma domestica" to the binary nomenclature of wild animals in question. However, in such cases where names for domestic animals already exist, the early given names should be used in the annex instead of "domestica". On the other hand, Dennler de la Tour recommended the addition of the term "familiaris" to the scientific names of the wild ancestors.

Animals↗
PubMed1992

Reptilian viruses: adenovirus-like agent isolated from royal python (Python regius).

An adenovirus-like agent was isolated from a moribund royal python (Python regius). The DNA containing virus replicated in IgH2-cells at 30 degrees C forming eosinophilic intranuclear inclusion bodies. The virus proved to be stabile to treatment with chloroform, pH3 und pH 12 but it was labile to heat (56 degrees C). Infected IgH2 cells revealed symmetric hexagonal virus particles measuring 67-79 nm in the nucleus. The isolate shared characteristics with the viruses of the family Adenoviridae.

Adenoviridae↗
PubMed1990

Fish viruses: transmission and pathogenicity of an icosahedral cytoplasmic deoxyribovirus isolated from sheatfish (Silurus glanis).

An iridovirus-like agent which was isolated from sheatfish fry showed to be of high virulence to those fish. 100% of sheatfish fry infected by water bath died within 8 days. The agent could be transmitted by infected fish causing 100% mortality in 11 days. Moribund fry showed spiralic swimming and exhibited hemorrhages in the skin. The infection studies demonstrate that the isolated virus was responsible for a sudden epizootic in 1988 with 100% mortality occurring in a warm water recirculating aquaculture unit in Northwest Germany, regularly controlled by the State Fish Epidemics Control Service of Lower Saxony and Fish Health Service.

Animals↗
PubMed1989

Fish viruses: isolation of an icosahedral cytoplasmic deoxyribovirus from sheatfish (Silurus glanis).

An icosahedral cytoplasmic deoxyvirus has been isolated from moribund sheatfish (Silurus glanis) fry of a commercial warm water recirculation aquaculture unit with cumulative mortalities of up to 100%. The agent replicated in BF-2 and in FHM cells at 20-30 degrees C producing cytoplasmatic inclusion bodies followed by lysis of the cells. The DNA containing virus proved to be labile to chloroform. Infected BF-2 cells revealed hexagonal particles in the cytoplasm measuring about 125-135 nm in diameter. The virus consisted of a central electron-dense core and a electron-translucent zone. The isolate shares characteristics with the Iridoviridae.

Animals↗
PubMed1989

Prevention of infectious diseases in aquaculture.

Infectious diseases remain one of the most important limitations to the successful propagation of aquatic animals. Most of the losses caused by pathogens in aquaculture could be prevented by health inspection, adequate environment and sound management practices. Effective control measures, mainly based upon 1) avoidance of pathogens 2) modification of the environment 3) improvement of host resistance 4) vaccination and 5) chemoprophylaxis are described.

Animals↗