Search PubMed⌕ Search

Biomedical subjects

Viroj Wiwanitkit

Publications and source records attributed to Viroj Wiwanitkit.

At least 73 records · Page 4Linked to original sources
PubMed2005

Management of acute renal failure due to Russell's viper envenomation: an analysis on the reported Thai cases.

Russell's viper (RVV) is an important tropical venomous snake. In severe envenomous cases, acute renal failure (ARF) can be seen. Here, the author performs this ministudy to document the management of ARF among reported Thai patients. A literature review on the papers concerning ARF due to RVV in Thailand was performed. Based on this study, hemodialysis and specific antivenom administration shows more favorable outcome than peritoneal dialysis.

Acute Kidney Injury↗
PubMed2005

Immune complex: does it have a role in pathogenesis of renal failure in dengue infection?

Dengue infection is a major public health problem. When acute renal failure complicates dengue infection, it is usually associated with severe disease as in dengue hemorrhagic fever or dengue shock syndrome. The role of immune complex in development of renal failure in dengue infection is still unclear. Here, the author used a computational medicine technology to study the property of the dengue virus-immunoglobulin complex. According to this study, the diameter of derived complex is much smaller, compared with the diameter of glomerulus. Entrapment of the immune complex is believed to occur when a previous glomerular lesion causes narrowing of the glomerulus's diameter. Therefore, the immune complex should not have a significant role in pathogenesis of renal failure in dengue infection.

Acute Kidney Injury↗
PubMed2005

A summary on the immunophenotyping of acute lymphoblastic leukemia in Thailand.

Numerous immunophenotypic features have been examined for their potential prognostic significance in predicting treatment outcome in leukaemias. These include the immunophenotypic subgroups of acute lymphoblastic leukaemia (ALL). However, only a few examples exist in developing Asian countries of the value of determining the immunologic characteristics of patients with ALL. The purpose of this study is to summarize the subtypes of ALL according to immunophenotypes recorded in previous reports in Thailand.A total of 263 documented cases were reviewed. Of these, 70, 42, 51, 10 and 90 patients had the phenotypes:- common, B, T, Null and Pre-B, respectively. The summative percentages for common, B, T, Null and Pre-B phenotypes are 26.6, 15.9, 19.4, 3.8 and 34.2%, respectively. According to this study, the three common phenotypes in general Thai ALL patients are Pre-B (34.2%), common (26.6%) and T (19.4%) phenotypes. After performing subtype analysis, the most common phenotypes among both adult and pediatric group is still Pre-B phenotype.

Humans↗
PubMed2005

Comparison for functional aberration of G-6-PD deficiency variants with exon 10 mutations.

Glucose-6-phosphate dehydrogenase (G-6-PD) deficiency is a common inherited enzyme deficiency in many parts of the world and there are many different variants described. Every G-6-PD deficiency variant has a unique underlying genetic defect, therefore it manifests specific properties. The single amino acid substitution in the globin chain is the commonest form of G-6-PD deficiency variant. Usually, the G-6-PD deficiency variant with the pathogenesis of a single amino acid substitution presents with only one aberration in secondary structure. Although many G-6-PD deficiency variants present similar structural abnormal points their functions sometimes are discordant. Here, the author performed a functional analysis on some alpha haemoglobinopathies using a novel bioinformatic tool, Polyphen. The mutations of five G-6-PD deficiency variants with exon 10 mutations, Guadalajara (386 Arg-->Cys), Beverly Hills (387 Arg-->His), Serres (361 Ala-->Val), Iowa (385 Lys-->Glu), and Clinic (405 Met-->Ile) were selected for further study in this investigation. According to the in silico mutation study, the functional change in the G-6-PD deficiency variants with exon 10 mutations studied is variable. Here, it indicates that the functional aberration in the G-6-PD deficiency variant is based on complex pathogenesis. The identification of the structural aberration only in a G-6-PD deficiency variant is not sufficient and should be supplemented with a further functional analysis for a better insight in this topic.

Amino Acid Substitution↗
PubMed2005

Modeling for tertiary structure of globin chain in Hemoglobin Suan-Dok disorder.

Hemoglobinopathies are an important inherited disorder with a high prevalence in Southeast Asia. Hemoglobin Suan-Dok is an example of a hemoglobinopathy that was first identified and described in Thailand. It has been identified as an unstable hemoglobin variant associated with alpha-thalassemia. The role of the hemoglobin instability in Hb Suan-Dok in the altered red cell morphology in comparison to the thalassemia-like deficit of alpha globin mRNA has not been entirely resolved and needs additional structural study for clarification. In this study the amino acid sequence of human alpha globin was extracted using ExPASY and compared with that obtained from the Hb Suan-Dok disorder. The derived sequences, alpha globin chains in both the normal and Hb Suan-Dok disorder, were used for further investigation of the tertiary structures. Modeling these proteins for the tertiary structure was performed using the CPHmodels 2.0 Server. For comparison the tertiary structure of human alpha globin chains in normal and hemoglobin Suan-Dok are calculated and presented. Based on this information, there was no significant difference between the predicted alpha globin tertiary structures of normal hemoglobin and Hb Suan-Dok. Therefore, from this study we can state that the tertiary structure of alpha globin is not significantly affected by the mutation in the Hb Suan-Dok disorder and that the effect of this hemoglobin abnormality may be silent. The data suggests that the thalassemic defect associated with the Suan-Dok mutation results from another unidentified process rather than the structural aberration and that the finding of a thalassemic picture might be due to another undetectable inherited hemoglobin disorder.

Hemoglobins, Abnormal↗
PubMed2005

Anti HCV seroprevalence among the voluntary blood donors in Thailand.

Hepatitis C virus (HCV) infection is an increasing problem, affecting large numbers of the population in both the developed and the developing parts of the world. It is generally accepted as a significant public health problem with major associated morbidities and mortalities, in particular hepatocellular carcinoma. One of several strategies for prevention of HCV transmission is screening for anti HCV serology among donated blood in the blood bank. However, screening for HCV infection varies considerably throughout the world; differences between resource-poor and resource-rich countries are particularly pronounced. This is a particular problem in the developing countries in Asia where HCV infection has high prevalence, this is especially the case among the underprivileged populations and those countries are usually less able to afford routine HCV serological screening in blood bank. In Thailand, HCV infection is an important infectious disease. However, screening for anti HCV serology is performed in only a few large blood banks. The purpose of this study is to summarize the prevalence of Anti HCV seropositivity among the voluntary blood donors in the previous reports in Thailand. This review identified 5 reports in the literature reporting data in 39,633 documented voluntary donors. In all there were 541 cases with Anti HCV seropositivity. The summative percentage for Anti HCV seropositive was 1.37%. This rate is similar to the high levels reported previously from many other Asian countries, implying the importance of HCV infection in Asia. Thorough donor screening to eliminate high-risk donors is recommended to improve blood transfusion services in Thailand and other developing countries and screening for Anti HCV serology should be set as the national strategies covering all blood banks.

Blood Donors↗
PubMed2005

Maternal C-reactive protein for detection of chorioamnionitis: an appraisal.

Premature delivery is still a significant problem in obstetrics, and chorioamnionitis is an unwelcome complication. C-reactive protein (CRP) is a circulating marker of low-grade inflammation and the role of its measurement in clinical practice remains unclear for many conditions. It has been claimed that estimation of CRP is helpful in the diagnosis of chorioamnionitis, and this study aims to appraise such claims. Following review of the literature, six reports were recruited for further metanalysis, including 466 cases. The overall prevalence of chorioamnionitis was 41% (191/466). The overall diagnostic activity showed sensitivity, specificity, false-positives and false-negatives of 72.8%, 76.4%, 23.6% and 27.2%, respectively. Therefore, we can conclude that estimation of maternal CRP is not helpful in the detection of chorioamnionitis, compared with standard investigations.

C-Reactive Protein↗
PubMed2005

Gene frequencies of the human platelet antigen-3 in different populations.

Human platelet antigen (HPA) systems consist of more than 12 biallelic antigen polymorphisms in which a base pair substitution leads to change in an amino acid of a glycoprotein expressed on the platelet. HPA-3 is a HPA that is mentioned for possible induction of neonatal alloimmune thrombocytopenia, posttransfusion purpura, and platelet refractoriness. A summary is presented of previous reports on the gene frequencies of HPA-3 among different populations. The frequency of HPA-3a and -3b ranges from 0.50 to 0.61 and 0.38 to 0.50, respectively. A significant correlation between the population ethnicity and the gene frequencies was detected in this study. However, it is quite difficult to use HPA-3 gene as a gene marker to determine the similarity of gene population in different populations. In addition, the comparison of the heterogenicity of HPA-3 frequencies to another well-known HPA gene, HPA-1 gene demonstrates that there is a greater variation in HPA-3 frequencies than in the HPA-1 gene. There was no significant correlation between the incidence of autoimmune thrombocytopenia disorder and the HPA-3 gene polymorphism pattern.

Alleles↗
PubMed2005

Study on the phylogenetic tree of human platelet glycoproteins.

Platelet glycoprotein is an important group of glycoproteins on platelets. Several types of platelet glycoproteins have been studied for their functions in the hemostasis system. A bioinformatic analysis was performed to find out how the platelet glycoproteins' genes are related to each other. A multiple sequence alignment phylogenetic tree was performed to present the family tree of the human platelet glycoproteins recorded in the genomic database, ExPASY. These derived sequences from the database were processed by ClustalW and subsequently used for preparation of the distance matrix by Phylip protdist. The final generated phylogenetic tree of human platelet glycoproteins was presented and discussed.

Blood Platelets↗