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Biomedical subjects

V Wagner

Publications and source records attributed to V Wagner.

At least 73 records · Page 4Linked to original sources
PubMed1984

Morbidity and immunological test in miners working in mines with low ionizing radiation level.

Total count of morbidity as well as prevalence of upper respiratory tract diseases (RTD) were followed in 210 miners working in shale mines with a low level of ionizing radiation regarding their smoking habits. After one and half year of observation were performed some tests of humoral immunity in the blood serum and saliva (immunoglobulins G, A, M and sIgA) as well as some tests of non-specific indices (alpha 2 macroglobulin -A 2M, transferrin -TRF, lysozyme -- LYS). The number of healthy non--smokers reached 41.3%, whereas the number of healthy smokers was only 26.5% (P less than 0.05). The greater was the number of diseases in single subjects, the greater was the difference between smokers and non-smokers (P less than 0.005). The difference between RTD morbidity of smokers and non-smokers was not significant in the subgroup of miners employed less than 10 years, but the length of employment it rose significantly (P less than 0.002) in disfavour of the smokers. The difference between smokers and non-smokers is emphasized by ageing. The mean levels of immunoglobulins (IgG, IgA, IgM) are significantly higher in healthy non-smokers in comparison with other miners; that is stressed also by distribution analysis. The distribution analysis. The distribution of A 2M values is also significantly different in the subgroup of healthy non-smokers compared with other miners. Highly significant differences were found between healthy non-smokers and other miners by discrimination analysis of coupled tests. The differences among the paired comperformed some tests of humoral immunity in the blood serum and saliva (immunoglobulins are apparently cooperative in the prevention of RTD. In contrast to that the level of A 2M are in reverse relationship to the levels of Ig; in the subgroup of healthy non-smokers low levels of A 2M are in connection with high levels of Ig of all three classes. The results are discussed from the point of view of the smoker's habits, the length of employment in mine's environment, the age of the miners and the supposed genetical factors.

Adult↗
PubMed1984

Seasonal changes in the relationship of blood calcium levels to immunoglobulins and some of the blood proteins in schoolchildren.

Blood and saliva were collected in the autumn and spring from a group of schoolchildren (39 girls, 35 boys) with a mean age of 11.4 years. Serum immunoglobulin IgG, IgA, IgM and IgE, alpha 1-antitrypsin (A 1-AT), alpha 2 macroglobulin (A 2M), transferrin (TRF), ceruloplasmin (CPL), lysozyme (LYS) and pertussis (PE) antibody levels were determined. Calcium (Ca2+) and total serum protein levels were also determined. Secretory IgA (sIgA) and secretory lysozyme (sLYS) levels were assessed in the saliva. A highly significant drop in Ca2+ levels was found in the spring in boys, while in girls there was only a greater scatter of the values. Mean IgG, IgA and IgM values fell significantly in the spring in both sexes, but IgE levels fell significantly only in boys. PE levels rose significantly in the spring in girls. Among the other proteins, all the values rose in boys, except for TRF, whose levels fell. In girls, LYS and TRF levels rose, but all the other values fell. The coefficients of correlation between Ca2+ and the tested proteins showed a significant relationship only for A 2M and PE in girls and only for the total protein level in boys; in boys, the determination coefficient for sIgA and IgM was over 10%. The results do not testify to the existence of a close relationship between blood Ca2+ levels and Ig and other blood protein levels.

Adolescent↗
PubMed1984

Prostaglandin E1 (PGE1) gastric tissue levels in the rat after truncal vagotomy and/or pre-hepatic portal hypertension.

Three groups of female Wistar rats were considered to study the pathophysiologic changes induced by truncal vagotomy and/or prehepatic portal hypertension. The first group was subjected to transabdominal bilateral truncal vagotomy (T.V.) and Heinecke-Mikulicz pyloroplasty, the second group to main portal vein partial stenosis (P.P.S.), and the third one simultaneously to T.V. plus P.P.S. The rats were sacrificed and the stomach homogenized. The separation and simultaneous measurement of gastric prostaglandin E1 (PGE1) resulted from high performance liquid chromatography (H.P.L.C.). Mass spectrometry (M.S.) was used for identification of several gastric PGE1 samples in purified extracts. Control rats were also sacrificed and examined. With regard to PGE1 gastric contents, the following results are reported: 1.2405 +/- 0.1356 micrograms/g (control rats), 0.786 +/- 0.1296 micrograms/g (T.V. rats), 1.8495 +/- 0.3433 micrograms/g (P.P.S. rats), and 1.2754 +/- 0.1154 micrograms/g (T.V. + P.P.S. rats). The interpretation of results is made at the moment on a hypothetical basis.

Alprostadil↗
PubMed1983

Indicators of humoral immunity in smokers and nonsmokers working underground.

A group of 215 underground shale miners (148 smokers and 67 nonsmokers) was examined for levels of immunoglobulins in sera (IgG, IgA, IgM) and saliva (sIgA), transferrin (TRF), alpha 2-macroglobulin (A 2M) and lysozyme levels in sera (LYS) and saliva (sLYS). The control group consisting of 95 healthy men was selected to match the age of the exposed miners. The mine environment was characterized by the presence of ionizing radiation, due to a geological configuration of the rock. The study revealed that the decline of IgG, IgA and sLYS levels in smokers was significantly deeper than in nonsmokers and controls. The levels of sIgA and serum LYS levels were increased both in smokers and nonsmokers. The change in the serum LYS and A 2M levels in miners did not depend on the smoking habits, except of A 2M levels in miners employed for more than 21 years. The differences were, as a rule, less pronounced in younger miners and tended to increase with the increasing length of work underground. The distribution analysis revealed some inter-miner differences in the susceptibility to these changes and the formation of smaller subgroups of miners with more or less subnormal test values, which supports the hypothesis on the heterogeneity of this population group. The tests showed that the statistical analysis of abnormal test values might play an important role in the assessment of the health risk in individuals. A simultaneous detection of subnormal values in some of pairs of tests may provide decisive data allowing a reliable differentiation between the afflicted miners and the healthy control subjects.

Adult↗
PubMed1983

Immuno-biochemical findings in groups of individuals occupationally and non-occupationally exposed to emissions containing nickel and cobalt.

Immune reactions elicited in the sera of individuals exposed to nickel and cobalt were assessed by changes in the concentration of serum immunoglobulins IgG, IgA and IgM and serum proteins alpha 2 macroglobulin (A2M), transferrin (TRF), alpha 1-antitrypsin (A1AT), ceruloplasmin (CPL) and lysozyme (LYS). Examinations were carried out in workers occupationally exposed to Ni (38 individuals) or Co (35 individuals) and in groups of non-occupationally exposed children living in areas with a different degree of air pollution from a nearby source of Ni and Co emissions (one group was made up of 54 exposed children, the other one of 64 "less exposed" children of the same age). Groups of non-exposed controls were represented by a group of 42 male adults matched by age and by a group of 48 children from a non-polluted area. Significantly increased average values were obtained for IgG, IgA and IgM in group of workers exposed to Ni, for IgA in workers exposed to Co and for A1AT, A2M, CPL and LYS in both groups of occupationally exposed adults (p less than 0.001 - p less than 0.005). Among non-occupationally exposed children the group of the most exposed had significantly elevated average values for A2M and A1AT which were higher than those recorded in groups of "less exposed" and control children (p less than 0.02 and p less than 0.05, respectively). The biomedical importance of these findings is discussed in detail.

Adult↗
PubMed1982

Age correlation of alpha-2-macroglobulin levels in healthy subjects.

Groups of 336 female and 310 male subjects from the age of 11 years to 94 and 86 years respectively were examined by radial immunodiffusion for their alpha-2-macroglobulin ( A 2M ) levels. Both groups were subjected to regression analysis, using a third degree polynomial, and the equations of the curves giving the best fit for the observed distribution of the values in relation to age were computed. The implications of the results for biology and medicine are discussed.

Adolescent↗
PubMed1982

Seasonal and sex-related changes in the levels of immunoglobulins and lysozyme in a semicohort of children during a three-year period.

A semicohort of 289 children of both sexes, initial age 9.5 +/- 0.4 years, was monitored in three urban localities of the Central-Bohemia Region over a period of three years. The children were examined twice a year, always after the summer and winter seasons; examined were: immunoglobulin (IgG, IgA, IgM) and lysozyme (LYS) levels in the blood serum and IgA and LYS levels in the saliva. The time series of six examinations revealed significant season-related fluctuations: peaks of mean IgG and IgM levels were detected after the summer months, mean LYS levels peaked after the winter season. The indicators monitored in the saliva did not show any significant season-dependent changes, but their values fluctuated considerably, with a highest decline at about 11 years of age. Intersexual differences were significant and also data from individual localities were found to differ. The distribution curves, constructed from data of three autumn and three spring measurements, revealed that the values of spring IgG and IgM levels differed significantly from those measured in autumn, showing a shift towards the lower values. No seasonal changes were observed in the levels of IgA, secretory IgA and LYS. The highest number of children with subnormal values was found in case of IgA, which is explainable by a physiologically occurring developmental deficiency of this type of immunoglobulin. The occurrence of subnormal IgG and IgM levels was markedly season-related, within a maximum in spring. The subnormal levels were significantly more frequently in boys.

Child↗
PubMed1981

The interrelations of some humoral factors of resistance with the prevalence of respiratory diseases in children. I. The differences among the protein levels in healthy and ill children.

The prevalence of respiratory diseases was followed in a group of 80 children (39 boys and 41 girls) of mean age 12.4 +/- 0.9 years during the part of school year (November - May incl.). The specimen of blood was taken from all studied children before the study started and the values of nine serum proteins were measured (IgG, IgA, IgM, IgE, alfa 1-antitrypsin = A 1-AT, transferrin = TRF, ceruloplasmin = CPL, alpha 2-macroglobulin = A 2M, lysozyme = LYS), as well as levels of two secretory proteins in saliva (sIgA and sLYS). The difference between the subgroup of boys, who fall into some respiratory illness during the observation time, and those who remain healthy, did not reach the level of significance. In contrast, the girls subgroup revealed significantly higher respiratory morbidity. With those girls, who remained healthy, the levels of serum IgE were highly increased and A 1-AT level weakly increased compared to ill girls. The level of TRF and CPL were significantly decreased with the healthy girls.

Blood Proteins↗
PubMed1981

The interrelations of some humoral factors of resistance with the prevalence of respiratory diseases in children. II. Interactions of humoral factors.

In a previous study the levels of nine serum and two salivary proteins were measured in a group of 80 children. Some significant differences of mean levels were found between the group of children, who remained well and who fell ill in the school year following the examination. In the present study the discrimination analysis was performed between both mentioned ill and healthy subgroups of children, referring to possible interaction of two protein tested. The comparison showed significant difference between double-protein combinations in 17 from 36 possibilities in girls subgroup. Relevant double combinations were assessed only in five of nine tested proteins: IgE, IgA, CPL, TRF and A 1-AT. Two combinations revealed the greatest significance: IgE with IgA, and IgE with CPL. No such significance was found in boys subgroup. The results suggest that prevalence of respiratory disease in influenced not only by previous levels of some single proteins, how it was found in the first paper, but also by the interactions of certain proteins.

Blood Proteins↗
PubMed1981

Correlations between blood lead concentrations and some blood protein levels in children residing in lead-polluted and control areas.

Ninety children residing in a highly Pb-polluted area near a lead foundry (group E), and 61 children from the control area (group C) were examined and compared. In group E children there were detected significantly increased blood Pb concentrations (girls: 31.6 +/- 16 microgram/100 ml; boys: 38.7 +/- 15.8 microgram/100 ml) in comparison with the control group C children (about 20 microgram/100 ml for both sexes). Regression analysis revealed significantly positive correlation of Pb concentrations in blood with the level of total blood proteins and ALAD, the correlation with A-1AT, alpha-2-M, TRF, and CPL was much less pronounced. Also the correlation of Ca2+ concentrations in blood with blood protein levels was not too marked. On the other hand, the age factor turned out to be very influencing in girls. Remarkable was the change of Pb and Ca correlation positivity in group C children to correlation negativity in group E (ALAD, total proteins, CPL), or of correlation negativity to correlation positivity (A-1AT, TRF). The observed changes are discussed from the aspect of multifactorial action of Pb in the organism.

Adolescent↗
PubMed1980

The morbidity of children with decreased serum levels of alpha 1-antitrypsin in an air pollution area.

In a group of 115 children, originating from an area with industrial air pollution, a subgroups of 13 (11.3%) children was found with markedly decreased levels of alpha 1-antitrypsin (A 1-AT). Most children of the concerned subgroups were girls (10/64 - 18.5%), and only 3/61 (4.9%) were boys. In comparison with another group of 120 children, originating from an unpolluted area, only 2 (1.7%) of children showed decreased levels of A 1-AT. Significantly decreased serum levels of IgM and Lysozyme were simultaneously found in the hypo A 1-AT children, whereas IgG, IgA and total blood proteinemia did not differ from the controls. Increased morbidity, especially for respiratory diseases, was found in the group with the A 1-AT decreased below the level of 1.5 mg/ml, in comparison with the children of the same area without a decrease of A 1-AT. The lowest morbidity was stated in the control from non-polluted area. The decreased A 1-AT levels were restored to the normal values after half a year in most of the afflicted children, with the exception of two girls. Hypothesis is suggested that markedly decreased A 1-AT values could be caused by environmental influences. This acquired deficiency is reversible in contrast with the well known genetical deficiency.

Adolescent↗