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Biomedical subjects

V Thomas

Publications and source records attributed to V Thomas.

At least 163 records · Page 9Linked to original sources
PubMed1976

Inorganic polyphosphate as an integral part of alkaline phosphatase preparations.

Alkaline phosphatase (from chicken intestinal sources) was shown to contain a considerable amount of polyanionic phosphorus which was released by basic digestion. The polyanionic phosphorus of alkaline phosphatase is not associated with protein or polyalcohols and does not exhibit a visible or ultraviolet absorption spectrum. Alkaline phosphatase and abiogenic inorganic polyphosphate were found to incorporate 32P-orthophosphate under similar experimental conditions. It has been previously reported that this enzyme will incorporate 32P-orthophosphate into its protein phosphoserine without the apparent concomitant utilization of an energy source. This reported phosphorylation was immediately reversible upon dilution of the phosphorylated enzyme with unlabelled orthophosphate, which indicates that the initial phosphorylation was an exchange reaction. These observations suggest that this polyanionic phosphorus from alkaline phosphatase may be inorganic polyphosphate.

Alkaline Phosphatase↗
PubMed1976

Acquired toxoplasmosis in Malaysia.

Two cases of acquired toxoplasmosis in asymptomatic Malaysian patients are described. In both instances the diagnosis was first made on the finding of the Piringer-Kuchinka reaction in excised lymph nodes from these patients and serological studies further confirmed the presence of hihg toxoplasmic antibody titres. The characteristic histological features of toxoplasmic lymphadenitis are discussed. Diagnosis and management of the disease are briefly reviewed with emphasis that the importance of diagnosing this disease goes beyond the establishment of a mostly self-limiting, clinically unimportant protozoan infection.

Adult↗
PubMed1975

Thick-smear Plasmodium falciparum antigen from in vitro cultures for the indirect fluorescent antibody test.

An important prerequisite for serological studies is the availability of specific antigens with which a high, consistently reproducible degree of test sensitivity can be obtained. In the present study, thick films of P. falciparum antigen were made from in vitro cultures in order to evaluate this antigen in terms of the sensitivity and reproducibility of the indirect fluorescent antibody test, as compared with an antigen prepared from washed infected erythrocytes of Aotus trivirgatus monkeys. The test was performed in 132 serum samples from 4 different sources. The results showed that the sensitivity and reproducibility of the two antigens compared well. No binding of donors' antibody to antigen was noticed within the 24-hour period of in vitroculture.

Antigens↗