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Biomedical subjects

V T Ivashkin

Publications and source records attributed to V T Ivashkin.

At least 37 records · Page 2Linked to original sources
PubMed2001

[Clinical significance of stress-protein content in peripheral blood lymphocytes of patients with myocardial infarction].

We studied the role of inducible stress-protein with molecular mass 70 kD (heat shock protein--HSP) in myocardial protection in ischemia. Content of HSP was measured in 25 patients with Q myocardial infarction (QMI) and 5 healthy controls. Western-blot was made on QMI day 1, 2, 7, 14 and 21, and once in the controls. Three types of HSP synthesis reaction manifested on QMI day 1. Type 1--HSP was absent in lymphocyte cytoplasm at 37 degrees C and after heat shock (HS); type 2--HSP accumulated after HS and was absent at 37 degrees C; type 3--HSP was present at 37 degrees C and after HS. Healthy subjects exhibited HSP after HS and the HSP synthesis was enhanced by HS. Type 1 was associated with favourable short-term prognosis of MI. Vast myocardial damage was accompanied with lowering synthesis with its activation at 37 degrees C by MI day 2. High synthesis of HSP on MI day 2-14 indicated moderate heart damage. Thus, HSP reactivity can be used as a factor of MI prognosis reflecting mobilization of intracellular defense proteins.

Adult↗
PubMed2001

[Cytokine production in patients with chronic viral hepatitis C during treatment with interferon-alpha].

Serum content of proinflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) and growth factors (GM-CSF, TGF-1 beta) and expression of CD14 and CD95 antigens on peripheral blood monocytes before and after 12-day therapy with alpha-interferon were studied in 25 patients with chronic viral hepatitis C (VHC). The concentrations of TNF alpha, GM-CSF, and TGF-1 beta were significantly increased (p < 0.05) and coexpression of CD14+ and CD95+ antigens on monocytes was increased by 61% in VHC patients in comparison with the control. After 3 months of therapy with alpha-interferon, the content of TNF alpha, GM-CSF, and TGF-1 beta essentially decreased and that of IL-6 increased; this was paralleled by improvement of clinical and laboratory parameters and decrease of coexpression of CD14+ and CD95+ antigens on blood monocytes. Modulation of the functions of immunocompetent cells and changed production of cytokines are apparently one of the mechanisms of inhibitory effect of alpha-interferon on HCV infection. Study of proinflammatory cytokines and growth factors in the serum and expression of CD14 and CD 95 antigens on monocytes can serve as additional tests for evaluating the efficiency of interferon therapy in patients with VHC.

Adolescent↗
PubMed2001

[Cytokine regulation of liver inflammation and fibrosis during chronic hepatic diseases].

Serum concentrations of antiinflammatory cytokines and growth factors were measured in 30 patients with chronic viral hepatitis (HCV), mainly HCV RNA, and 10 patients with liver cirrhosis (LC), classes B and C according to Child-Pew. Serum concentrations of tumor necrosis factor-alpha (TNF alpha), granulocytic-macrophagal colony-stimulating factor (GM-CSF), and growth-transforming factor-1 beta (TGF1 beta) were increased in 63.86 and 80% patients with HCV and LC, respectively, and differed significantly (p < 0.05) from the control. The level of TNF alpha positively correlated with the concentration of alanine aminotransferase (r = 0.14). A positive correlation between TGF1 beta and histologic activity index was detected (r = 0.16). Increased levels (p < 0.05) of IL-5, TNF alpha, and TGF1 beta were detected in LC patients in comparison with the control. Patients with LC concomitant with anemia had higher (though not significantly) concentrations of TNF alpha in comparison with patients without anemia. Increased levels of proinflammatory cytokines and growth factors in the sera of patients with HCV and LC indicate activation of immunocompetent cells, including mononuclear phagocytes. These data are in line with experimental findings, indicating an important role of the studied cytokines in the development of hepatic inflammation, fibrosis, and cytopenic syndromes.

Adolescent↗
PubMed2000

[Nitric oxide synthesis in patients with myocardial infarction].

The findings of the author demonstrate that the system of nitric oxide (NO) generation and metabolism is an element of multicomponent response of the organism to myocardial infarction (MI). This response consists in MI patients' systemic ability and, in particular, their peripheral blood mononuclears' ability to produce NO as well as the absence of iNOS activation in peripheral blood of MI patients. It is important for a practitioner to understand that a fall in the urine and plasma concentration of NO metabolism end product reflects low activity of NO generation while NO is a powerful regulatory factor in the cardiovascular system. Thus, low levels of NO and its metabolites in the urine and plasma of MI patients indicate depletion of compensatory coronarodilatating potential and, eventually, poor prognosis. Relevant measurements will provide additional parameters in assessment of body reserves in MI patients and in MI prognosis within the first hours of its onset.

Adult↗
PubMed2000

[Hematological syndromes in patients with chronic hepatitis C].

AIM: To examine hemopoiesis and to estimate proinflammatory cytokines in blood serum from 42 patients with chronic viral hepatitis (CVH) associated with 1-3 lineage cytopenia in the blood. MATERIAL AND METHODS: 42 patients with diagnostically complicated hematological picture suggestive of hemoblastosis were examined using standard tests, bone marrow puncture and trepanobiopsy, laparoscopy with biopsy of the liver and spleen, transcutaneous puncture biopsy of the liver under ultrasonic control, explorative laparotomy with splenectomy and liver biopsy and, on demand, laboratory tests for hemolysis, presence of antithrombocytic and antileukocytic antibodies, iron metabolism, karyological analysis of bone marrow cells was also made. RESULTS: The majority of the patients were diagnosed to have chronic hepatitis C with low activity of hepatic inflammation and frequent (55%) absence of the diagnostic antibodies to hepatitis C virus in the serum. In 86% of cases blood cytopenia reflected uneffective hemopoiesis and in 14% of cases hemopoiesis hypoplasia was found. CONCLUSION: Uneffective hemopoiesis, high content of immune response cells-effectors in the bone marrow and high concentration of TNF in the serum indirectly evidence for a pathogenetic relationship of chronic HCV infection with cytopenic hematological syndromes.

Adult↗
PubMed1999

[Role of 24 hour intraesophageal pH monitoring in diagnosis of gastroesophageal reflux and assessment of drug effectiveness].

58 patients with gastroesophageal reflux (GER) and 18 patients with chronic gastroduodenitis (CGD) were examined using 24-h monitoring of intraesophageal pH. GER patients exhibited changes in all the readings of 24-h pH-gram of the esophagus which strongly correlated with severity of clinical symptoms (heartburn, metasternal pain), reflux-esophagitis (endoscopical picture), data of esophagotonocimography. 24-h monitoring of intraesophageal pH provided an adequate estimation of the drugs used to treat GER. As to effect on intraesophageal pH, domperidon had no effect, famotidine had a moderate positive effect, omeprasol returned pH to normal.

Adult↗
PubMed1999

[Use of biologically active food additive cardiohels in the treatment of patients with ischemic heart disease].

Biologically active food additive (Inrich production) cardiohels was added to the diet of ischemic heart disease patients with hypertension and chronic heart failure stage IIB receiving basic medication with hypotensive, coronarolytic drugs and cardiac glycosides. As shown by control 24-h blood pressure and ECG monitoring, echocardiography, cardiohels has a mild hypotensive effect, improves coronary circulation and microcirculation, myocardial contractility. Mechanism of these positive effects are discussed. Cardiohels is recommended for patients with ischemic heart disease as it allows to reduce the dose of hypotensive and coronarolytic drugs by 35% and cardiac glycosides by 25%, thus lowering the risk of relevand side effects.

Female↗
PubMed1997

[The therapeutic efficacy of interferon-alfa-2a (Roferon-A) in chronic hepatitis C].

THE AIM OF THE STUDY: Evaluation of 6-month treatment with roferon-A of patients with chronic hepatitis C (CHC) and comparison of the treatment regimens. MATERIAL AND METHODS: 79 CHC patients received roferon-A for 3 months in a dose 6,000,000 IU 3 time a week. In case of the response to treatment was continued for the next 3 months (3,000,000 IU 3 times a week). Clinical-laboratory findings, results, of EIA and liver biopsy histology were examined. RESULTS: Primary remission was achieved in 71.8% of cases. 36.9% of patients developed recurrences including 14.1% recurrence arising in the course of therapy. Stable remission was obtained in 32.4% of patients. 28.2% patients were non-responders. Side effects were mild, discontinuation of the treatment was necessary only in 7% of cases. CONCLUSION: Roferon-A administration in a dose 6,000,000 IU for 24 weeks is optimal both as related to cost and efficacy of the treatment.

Adolescent↗