Search PubMed⌕ Search

Biomedical subjects

V Rusu

Publications and source records attributed to V Rusu.

115 records · Page 7Linked to original sources
PubMedDate not supplied

[Fluorescence anisotropy--a parameter for characterizing membrane fluidity].

The lipophilic fluorescent probe diphenylhexatriene (DPH) has been shown previously to behave as a marker of plasma membrane in living cell systems, and it is therefore been widely used in membrane fluidity studies via fluorescence anisotropy measurements. The anisotropic coefficient, which is inversely related to the rotational motion of the probe in membrane phospholipids, was significantly higher at 37 degrees C than at 23 degrees C for 9 series of red blood cells ghosts obtained from three healthy subjects. We also have studied the importance of the nature of two different polaroid films which permits the observation of fluorescence polarization.

Diphenylhexatriene↗
PubMedDate not supplied

99mTc MIBI in neurofibromatosis imaging diagnosis: case report.

99mTc MIBI has been shown to accumulate in different cancer cells types, in vitro and malignant tumours in vivo, making evidence of the scintigraphic diagnostic of them. We report two cases of neurofibromatosis type I, which has realised high levels of 99mTc MIBI uptake, primary to all chemo- or radiotherapy. Only certain zones of tumorous localisation have been seen, but a relation with the lesion histology was not really established. In conclusion, 99mTc MIBI scintigraphy may be usefull in the investigation of type I neurofibromatosis.

Adolescent↗
PubMedDate not supplied

[Complementary scintigraphic examinations (99mTc-MDP, 99mTc-MIBI and Octreoscan) in breast neoplasm assessment].

Scintigraphic imagery was used in breast carcinoma mostly for the bone metastases screening, using 99mTc-MDP. Others radiopharmaceuticals can be used to visualize either the primary tumour or the soft tissues metastases. It's mainly the case of 99mTc-MIBI, and also of some somatostatin analogues, like Octreoscan. This case report of a patient with a T3N1M+ breast adenocarcinoma having different secondary sites represent also a prove of the complementarity of these explorations, which give the possibility of diagnostic and therapeutic evaluation and, in the same time, the possibility of a better understood of the metastasizing mechanism.

Adenocarcinoma↗
PubMedDate not supplied

[Thyroid cancers].

We have analysed factors that influenced on the patient survival in 195 cases of thyroid cancer. The survival is essentially influenced by the histology, the papillary forms (survival after 15 years: 83.1-93%) and follicular cancers (survival after 15 years: 84-94%) having an excellent evolution. The non-differentiated and mixed cancers have a very severe prognosis that is independent of the complex therapy that have been used (survival after 5 years: 17.1% in non-differentiated forms and 32.5% in mixed ones). The prognosis is better in subjects under the age of 45 and in female subjects. The cancers in the 1-st and 2-nd stage of tumor extension have a better evolution that those in the 3-rd stage. In differentiated cancers, the total or subtotal thyroidectomy followed by radioiodine administration determine the best survival.

Adenocarcinoma, Follicular↗
PubMedDate not supplied

[An in-vitro study of 99mTc sestamibi uptake by normal and neoplastic cell lines].

The hexakis (2-methoxy isobutyl isonitrile) technetium (I) (99mTc MIBI) is a representative molecule for a class of monocationic lipophile 99mTc compounds, knowing to be initially used like myocardial scintigraphic agents, and now also in oncological scintigraphy. This present study aims to compare the in vitro 99mTc MIBI cellular uptake on some normal and neoplastic cells. Three tumoral cell lines were used: M4Beu (pigmented melanoma), M3Dau (pigmented melanoma), MCF7 (breast cancer), in comparison with newborn rat cardiac myocytes and fibroblasts. Monolayers cultures cell were incubated for 1 hour with 37 kBq of 99mTc MIBI. Our results: (a) confirm a preferentially 99mTc MIBI uptake on the myocytes in comparison of the fibroblasts; (b) show that this uptake is very different on the neoplastic studied cultures cells, depending on the cell type (higher on M3Dau in comparison of the other two malignant cell lines, and also higher than the myocytes. The neoplastic cellular mechanisms to accumulate 99mTc MIBI, are only hypothetically understand; there are thought to be also in relation with the Nernst equations, applied at the plasmatic and mitochondrial membranes level, like in normal cells. A correlation with some phenotypic neoplastic cells characteristics, in relation with the malignancy tumoral potential seems possible. If there is the case, this can be a possible explanation of some in vivo imaging results, obtained with 99mTc MIBI, in oncologic scintigraphy.

Animals↗