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Biomedical subjects

V Rusu

Publications and source records attributed to V Rusu.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

TLC characterization of liposomes containing angiotensinogen, angiotensine I, angiotensine II and saralazin.

Recent studies have described intracellular binding sites for angiotensine II. In vascular smooth muscle it was found that intracellular injection of angiotensin II increases the Ca2+ level. An alternative method for intracellular delivery of drugs is represented by using liposomes. Thus, the aim of this study was to characterize liposomes filled with angiotensinogen, angiotensine I (Ang I), angiotensine II (Ang II), and saralasin by a TLC method and examine the physiological effects of these on rat vascular smooth muscle. Ang II (for all concentrations tested in the aqueous phase) and Ang I (for concentrations less than 10(-4)M) did not affect the thin-layer chromatography migration of this type of vesicle, suggesting that dose-dependent effects on physio-pharmacological experiments could be studied. On the other hand, this type of experiment could not be performed for salarasin- or angiotensinogen-filled liposomes. Administration of liposomes containing Ang II (10(-6)M), Ang I (10(-6)M), angiotensinogen (10(-6)M) and saralasine (10(-6)M) caused the contraction to isolated rat aorta smooth muscle, suggesting the presence of active intracellular binding sites.

Angiotensin I↗
PubMedDate not supplied

[The contribution of molecular biology to the progress of the physiological sciences].

Life science, physiology have an important improvement on cell sciences, by seeing cell through a complex integrative concept. So, the notion of homeostatic equilibrium for the whole body is applied at the cellular level. Also, molecular biology techniques can facilitate the answer for a lot of the physiological and pathological questions. This paper mention only some of the many examples which justify both the importance of molecular biology for the cognitive progress of physiological and biomedical sciences and the role of physiological notions for the complexity view of molecular sciences.

Animals↗
PubMedDate not supplied

Technical and biophysical aspects in prosthetic occlusal rehabilitation for edentulous teenagers.

The loss of anterior teeth may be transient solved by temporary adhesive bridges (Oslo bridge, Rochette, Ellmann, Neumann, Tschemitscheck, Armatron systems). Provisional crowns and bridges could be used for Brackets fixing. Anterior bridge with subocclusal connector permits transversal expansion. When a successful orthodontic treatment is followed by a prosthetic treatment, it is not important to change the achieved occlusion, by the occlusal framework morphology. The framework design of the metal component can contribute to the improvement of the aesthetic aspect and reduce the cost by using a small quantity of expensive alloy. Cytocompatibility of the used Cr-Co alloy is confirmed by cell culture test.

Adolescent↗
PubMedDate not supplied

[Insulinemia and its evolution over time in obesity].

There is a steady-state metabolism of adipose tissue ("adipose state") with a "metabolic signal" significance. It consists of: seric levels of glucose, insulin, free fatty acids. We studied 45 cases with uncomplicated obesity and we correlated seric level of insulin, basal and reactive, with DAM (mean adipocyte diameter), insulin receptor activity and obesity evolution length (age of obesity). Level of insulin has high influence on insulin receptor function (p < 0.01). Reactive insulin level are higher with obesity age (p < 0.0001). Hyperinsulinemia is a constant, continuous and early reference point of obesity.

Adipose Tissue↗
PubMedDate not supplied

[Mechanotransduction and tensegrity (I)].

The current review deals with mechanotransduction by means of an architectural model of cell function called tensegrity. This concept was introduced by D. E. Ingber in order to frame more focused mechanisms of mechano-transduction, i.e. different signaling pathways, which are less able to predict global cellular behaviour in response to stress, into a coherent mechano-chemical theory of cell function. Tensegrity structures are made and held up by interconnecting a continuous series of tension elements with a discontinuous series of compression resistant struts, in a simple "stick and string" model. These structures develops an intrinsic stabilizing tension called prestress and reacts by global rearrangements of their configuration to a local action of a mechanical stress. The only requirement of a tensegrity network is that tension is continuous and compression is local. At the cellular level the theory assumes that membrane, nucleus and all the organelles are hard-wired by the insoluble cytoskeletal (CSK) scaffold. More than that, the interconnection between CSK and the extracellular matrix (ECM) provides an efficient mechanical couple responsible for changes in cell shape and movement. Cell shape, in turn, regulates cellular function. Focal adhesion complexes, which mediate the CSK-ECM interaction are viewed as integrative devices for both mechanical signaling and soluble factor-dependent signaling. Furthermore, the tensegral molecular network is considered to be a solid-state regulatory system of all cell functions. The way tensegrity influences molecular mechanics, cellular response tuning and coordinated behaviour of large scale structures are also discussed. Besides its conceptual importance, the tensegrity model has multiple applications, being used in combined approach of cell biology, bioengineering, architecture, and biomechanics in order both to improve cell culture and its subsequent applications and the open the way to a more complex technology of tissular engineering.

Animals↗
PubMedDate not supplied

[Whole-body bone scintigraphy in the diagnosis and follow-up of the evolution of breast cancer].

UNLABELLED: We have studied 75 patients, diagnosed with breast cancer, (39 on the right, 36 on the left and 4 on the two breasts), the TNM classification being: stage I--13.3%, stage II--36%, stage III--40% and stage IV--10.6%. Each patient has undergone a whole body scan, 2 hours after a 20-25 mCi 99mTc MDP i.v. dose, using a Siemens Diacam gamma camera. Three parameters where assessed: 1) the distribution of multiple metastasis bone sites; 2) an index, the counts/pixel ratio between the metastatic site interest region and a homologous normal bone interest region (IMDP); 3) a graphic representation of the metastasis heterogeneity radiotracer uptake. RESULTS: Bone metastasis have been found in 23 patients (30.6%), 16 cases having multiple sites; the rate between right and left breast cancer, for these metastases, was 13:10, according with the previous mentioned breast frequency. From all the 23 cases, the most involved site was the ribs (18 cases), followed by the spine (in 17 cases), the iliac bone (9 cases), the femur (7 cases), the skull (3 cases). On the spine, the more frequent involved was the dorsal spine (49.23%), than the lumbosacral (26.13%) and last the cervical spine (12.3%). On the dorsal vertebrae, in 91% the metastasis were localized between D8 and D12. The highest values of I-MDP characterize the femoral metastasis. The heterogeneity was high only for the vertebrae multiple sites. In conclusion, our study show pattern characteristics on the distribution and also on the uptake radiotracer bone metastasis quantification in breast cancer, in respect with others neoplasm's bone metastasis.

Adult↗
PubMedDate not supplied

Direct evaluation of erythrocyte aggregability in human, sheep and cow blood samples with a computerized image analysis.

The non-Newtonian properties of blood are caused by the unique properties of red blood cells to aggregate and to deform. Aggregation mechanism of erythrocytes contributes significantly to blood viscosity. Techniques for the assessment of red cell aggregability have major implications for medical practice. A number of techniques based on various principles have been proposed by many researches. However, as yet, none of these techniques have been applied on a wide basis. The aim of the present paper is to determine red cell aggregability in sheep, cow and human blood samples. The red cells remain in the their natural environment (their own blood plasma). First, we had to transform an IBM-PC computer into an Image Acquisition System, so we designed a frame-grabber and the soft driver necessary to assure the image acquisition taken by the TV camera by means of the microscope. These images are digitized, transformed in a standard format BMP and can be compressed and stored or processed. The entire system software is written in C++ assembly language. To evaluate the aggregability index in the blood sample was determined the total area occupied by all automatically detected erythrocytes (Stot) and the overall area occupied by the aggregated erythrocytes (Saggreg). . EAI = (Saggreg/Stot) x 100%; EAI shows the tendency of red blood cell to form aggregates.

Algorithms↗
PubMedDate not supplied

[Rapid methods useful for determination of streptococcal groups with special reference to streptococci of groups B and D].

A study was carried out on the efficiency of certain rapid methods for the identification of streptococcal groups, especially groups B and D, methods based upon physiologic properties such as hippurate hydrolysis, production of pigment, esculin hydrolysis, tolerance to salt and bilolysis, associated with the classical tests, for instance the hemolysis type and sensitivity to bacitracin and optochin. By these tests, accessible to hospital laboratories, a number of 3083 streptococcal strains were correctly identified within 2 to 18 hours, of which 2567 group A, 31 group B, 184 group C, G, 113 group D, 155 Str. pneumoniae and 31 viridans, strains isolated from pathological products.

Bacitracin↗
PubMedDate not supplied

[Preoperative and intraoperative scintigraphic detection of sentinel node].

It is known that the malignant primary tumor site has a specific lymphatic drainage, all the time intermediate, however, by a first lymph node named sentinel node. This can be situated in some typical or atypical topographic area and can be or not clinically evident. If the sentinel node is not invaded by the metastatic cells there is no metastatic risk for other lymphatic nodes, so the surgical treatment is not necessary to involve the corresponding lymphatic area dissection. An imagine, noninvasive detection of the involvement of the sentinel node in the spread of cancer could be related with the surgical decision to perform (or not) fully regional lymphadenectomy (based on biopsy result of such positive detected nodes) allowing only a tumorectomy in the first stages of neoplasia. For the case of scintigraphic method to detect the sentinel node, the performance depend on the development of radiotracers, the modification of the lymphoscintigraphic mapping techniques protocols, and the development and improvement of detectors.

Humans↗