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Biomedical subjects

V Puri

Publications and source records attributed to V Puri.

At least 91 records · Page 5Linked to original sources
PubMed1981

Circulatory shock associated with purulent peritonitis.

The relative roles of bacterial infection, fluid loss and myocardial failure were investigated in 24 patients in whom circulatory shock appeared as a complication of purulent peritonitis. The 13 acute survivors, including 6 hospital survivors, had strikingly lower initial plasma volumes and total blood volumes than the 11 patients who died. Differences in blood volume were not explained by differences in previous treatment or in duration of peritonitis. Acute survivors promptly improved after fluid repletion, whereas the patients who died failed to respond to the infusion of equivalent volumes of fluid. In contrast to acute survivors, the fatal cases demonstrated disproportionate increases in both right- and left-sided filling pressure, increases in pulmonary vascular resistance and decreased right and left ventricular work capability. These observations in patients complement experimental studies in which biventricular cardiac failure was implicated in the fatal progression of septic shock.

Adolescent↗
PubMed1981

Serum levels of testosterone, cortisol, prolactin and bioactive luteinizing hormone in adult male rhesus monkeys following cage-restraint or anaesthetizing with ketamine hydrochloride.

The present studies were carried out to characterize and compare the acute effects of cage-restraint or administration of a mild anaesthetic on serum levels of testosterone (T) cortisol (C), prolactin (Prl) and bioactive luteinizing hormone (LH) in adult male rhesus monkeys. Serum T levels declined progressively while C levels increased in the 3 sequential blood samples obtained at 20 min intervals from restrained monkeys. Prl and LH levels in the serum remained unaltered. Following single or multiple injections of ketamine hydrochloride (10 mg/kg) serum T levels did not decline progressively as seen in the blood samples obtained from the cage-restrained, conscious monkeys. Serum C and Prl levels showed a progressive rise following anaesthetization. LH levels were not affected by the anaesthetic. The findings reported here are of pertinent relevance to the evaluation of acute effects of any experimental procedure aimed at altering circulating levels of the hormones studied.

Animals↗
PubMed1980

Testosterone, cortisol, prolactin and bioactive luteinizing hormone in day and night samples of cerebrospinal fluid and serum of male rhesus monkeys.

Testosterone (T), cortisol (C), prolactin (PRL) and bioactive luteinizing hormone (bLH) were found to be normal constituents of the cerebrospinal fluid (CSF) of all the 15 adult male rhesus monkeys studied. The CSF levels of the hormones showed a good correlation with their serum levels. The geometric mean values of circulating levels of T, PRL, bLH in all the animals studied were significantly lower in the samples of the two body fluids collected between 09.00 and 11.00 h as compared with those collected between 21.00 and 23.00 h. C levels were higher during the day as compared with the night samples. This marked difference between the day and night levels of the circulating hormones was not observed in a few individuals which suggests that the diurnal changes in circulating levels of these hormones may not occur as a rule in all rhesus monkeys. The serum:CSF ratios for C, PRL and bLH did not vary significantly between the day and night samples of the body fluids as they did for T. This suggests that T is poorly transferred from the blood to the CSF as compared with the other 3 hormones studied. The possible pathways by which the hormones are transferred into the CSF and the functional significance of their presence in the CSF are discussed.

Animals↗
PubMed1980

Bioactive luteinizing hormone in the plasma and cerebrospinal fluid of female rhesus monkeys.

An in vitro bioassay method using Leydig cells from mouse testes has been validated for quantitating lutenizing hormone (LH) in the plasma and cerebrospinal fluid (CSF) of normally menstruating and ovariectomized rhesus monkeys. In all six normally menstruating monkeys studied, the mid-cycle peak of plasma LH coincided with the estradiol peak in blood samples taken at 24-hour intervals. Blood samples collected at 4-hour intervals during the periovulatory period showed multiple peaks of both bioactive LH and estradiol, and the major estradiol peak preceded the major bioactive LH peak by about 16 h. The plasma progesterone levels were about 1 ng/ml at the time of LH peak. Bioactive LH was detected also in the CSF samples of both intact and ovariectomized monkeys. The levels of LH in CSF were not elevated in the gonadectomized monkeys although their plasma LH levels were markedly increased.

Animals↗
PubMed1979

Biologically active luteinizing hormone (LH) in plasma. V. A re-analysis of the differences in the ratio of biological to immunological LH activities during the menstrual cycle.

The relationship between the biological and immunological activities of human luteinizing hormone (hLH) in plasma collected from female subjects was examined. The biological activity was measured by an in vitro bioassay and the immunological activity by an hLH radioimmunoassay (RIA), using improved reagents, such as the 1st IRP for human pituitary LH for immunoassay (code No. 68/40) as standard, a subunit-free biologically active iodinated hLH preparation as tracer and an anti-hLH serum of relatively high specificity. Similar profiles of biological (B) and immunological (I) activity were obtained in the plasma samples collected daily throughout 40 menstrual cycles (5 cycles from each of 8 subjects). However, the B/I ratios were significantly lower during the period of LH surge (P less than 0.001) than throughout the remainder of the cycle. The within- and between-assay variation in B/I ratios was investigated by the simultaneous assay of biological and immunological activities in plasma pools obtained by combining equal aliquots of plasma from each daily sample of the menstrual cycle from each of 5 cycles of each of 4 subjects. The analysis of these 20 pools revealed highly significant individual differences in B/I ratios, ranging from 0.81 to 1.33. The coefficient of variation was 20% between-subjects and 5% within-subjects. There was no seasonal variation in B/I ratios. That the individual differences in plasma B/I ratios were not attributable to the procedure of pooling was ascertained by the simultaneous assay of both activities in parellel in daily plasma samples and in the pools formed from these samples from three complete cycles. Thus the analysis of the differences in B/I ratios obtained throughout the menstrua- cycle revealed three major sources of variation. The first occurs in the form of generally elevated (higher than unity) B/I ratios, the second consists of a significant drop in B/I ratios during the midcycle LH surge, and the third source is represented by the significant between-subject differences. It is concluded that the first source is attributable to the relatively higher levels of "impurity" (i.e. biologically inactive, immunologically active material) in the standard preparation compared to those present in plasma of biologically inactive, immunologically active material of unknown composition and origin. If so, the latter source limits the quantitative significance of the RIA procedures employed. It is suggested that these three sources of variation account for most of the differences in B/I ratios for plasma lLH reported in the literature.

Adult↗