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Biomedical subjects

V Moennig

Publications and source records attributed to V Moennig.

At least 109 records · Page 6Linked to original sources
PubMed1978

Further investigations on the porcine lymphoma C-type particle (PLCP) and the possible biological significance of the virus in pigs.

The possible biological significance of the procine lymphoma C-type particle (PLCP) was investigated serologically. The screening of 1 200 field sera of apparently normal breeder pigs, performed with an immunodiffusion test, revealed negative results. The same results were obtained with sera from a herd with hereditary lymphosarcoma and with sera from experimentally infected piglets. In cases of doubtful precipitation lines additional tests with a more sensitive indirect immunofluorescence assay were performed. In no case a specific cytoplasmic fluorescence could be observed. The same sera of the 1 200 pigs were tested with BLV antigen as well as 350 bovine sera from BLV-positive cattle were tested with PLCP antigen. A possible antigenic relationship between BLV and PLCP could not be detected by the assays mentioned above. Purified PLCP was compared in SDS-PAGE with MuLV, FeLV, and BLV. The electrophoretical pattern presents suggestive evidence for a typical oncornavirus polypeptide composition. Apart from the presumable major internal protein with a molecular weight of 28 000 d, three low molecular weight proteins appeared at 12 000 d, 11 000 d, and 10 000 d. High molecular weight proteins could not be detected yet. The antigenic specificity of these bands has still to be confirmed.

Animals↗
PubMed1975

Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus.

Treatment of Friend leukemia virus gp71 with protease-free glycosidase enzymes results in removal of the major portion of the carbohydrate without affecting the amount of protein present. The digested material migrates as protein of about 60,000 to 65,000 molecular weight on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Analyses of the serological properties of gp71 after enzyme treatment indicated that the type, group, and interspecies determinants were not destroyed. In contrast, treatment with proteolytic enzymes led to the complete destruction of the gp71 molecule, including the total elimination of its serological reactivity as measured by direct and competition radioimmunoassay and by a serum cytotoxicity assay. We conclude that the carbohydrate portion of gp71 is not of major significance in defining the antigenic determinants of this viral glycoprotein.

Carbohydrates↗
PubMed1975

Morphological, chemical, and antigenic organization of mammalian C-type viruses.

New features in the architecture of mammalian type C viruses, in particular knoblike surface projections and hexagonally arranged subunits on the core shell could be demonstrated by electron microscopy, taking advantage of newly developed preparation techniques. As examples, murine leukemia viruses (MuLVs) and newly isolated porcine and bovine C viruses are presented. The major proteins of a MuLV were isolated and partially characterized in chemical terms and with respect to their serological and other biological activities, such as interfering and hemagglutinating (HA) capacity. Most of the characterized proteins could be localized in particular substructures of the virion either by selective removal or isolation of electron microscopically identifiable constituents. The information obtained allowed the design of a more detailed model of mammalian C viruses. Special attention was devoted to the further characterization of interspecies antigens of mammalian C viruses. Different antigenic determinants were revealed. Their distribution allows further subgrouping of mammalian C viruses.

AKR murine leukemia virus↗