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Biomedical subjects

V Markova

Publications and source records attributed to V Markova.

At least 19 recordsLinked to original sources
PubMed2005

[Role of intercellular adhesion molecule-1 and E-selectin in the pathogenesis of ovarian hyperstimulation syndrome].

OBJECTIVE: To explore the ICAM-1 and E-selectin in patients with OHSS and clarifying its role in pathogenesis of the syndrome. MATERIALS AND METHODS: 20 patients were included in the research and 20 control patients with stimulating ovarian cycle for the purposes of ART, during the period from 01.01.2004 till 01.01.2005 years in the center of ART-MU-Varna and Department of Obstetrics and Gynecology-IVF-ward-MU-Ben-Gurion, Israel. The patients were divided into 3 subgroups according to the degree of OHSS by the classification of Golan- mild, moderate, severe. The method of ELISA was used to record the values of the factors under research. RESULTS: Six patients with severe OHSS, showed increase in the value of the ICAM-1 and somebody of them, showed low level of E-selectin testing in serum and ascitic fluid in comparison with the control group and the rest of the patient with OHSS. CONCLUSION: OHSS is a life-threatening complication in which the main pathophysiology factor is the increased of capillary permeability. ICAM-1 are expressed on human granulosa cells and by the vascular endothelium after the stimulation by inflammatory cytokines and acts as a mediator of the cohesion with the lymphoid cells. E-selectin is produced by the endothelium after cytokine activation. The soluble forms of these molecules are found in serum, follicular fluid during the COH, as well as in ascitic fluid in severe OHSS. In our research adhesion molecules- ICAM-1 are correlated to the OHSS, especially in the severe forms of the syndrome.

Adult↗
PubMed2004

[The importance of the sonographic control for a successful embryotransfer after in vitro fertilization].

Two groups of patients have been assessed for a period from January 2000 till Mai 2004. In the first group of 222 patients the Embryotransfer was performed without sonographic control. In the second group of 253 patients the transfer was sonographically controlled. The results of the second group - 54,1% pregnancy rate per transfer are statistically better than of the first group - 29,3% pregnancy rate. ET performed under sonographic control improves significantly the results.

Abortion, Spontaneous↗
PubMed2003

[Diagnostic and clinical status of premature ovarian failure].

Premature Ovarian Failure is a heterogeneous disorder with numerous causes. The exact prevalence of POF is unknown. In this study, we want to make Differentiate diagnosis of hypergonadotropic amenorrhea and to determine the difference between them. Retrospective, we follow up 475 women at which we perform ovarian stimulation and we observe that 6 (1.3%) have Gonadotropin resistant ovary syndrome in IVF-centre--MU--Varna.

Adult↗
PubMed2003

[Is there a correlation between the vaginal colonization with group B streptococci and premature deliveries?].

The study covered 110 pregnant females during the period from January 1, 2002 till April 30, 2003. Nine females (8.18%) delivered premature newborns. Samples were taken from the posterior vaginal fornix and canalis cervicalis in the 18th and 24th gestational week. The preparations stained after Gram were examined under light microscope. Microbial cultures on blood agar were examined, too. It was established that 18 or 16.36% of all the cases presented with cultures positive for group B streptococci (Str. agalactiae) and Ureaplasma urealythicum. Some other microbial flora representatives such as Chlamydia trachomatis, Neisseria gonorrhoea and Trichomonas vaginalis were additionally identified. The independent colonization with Str. agalactiae prior to the 18th gestational week does not relate to the spontaneous abortions and premature deliveries at all. On the other hand, the colonization with Group B streptococci or their symbiosis with other microorganisms after the 23rd-24th gestational week displays a certain correlation with the premature births.

Adult↗
PubMed2003

[Some aspects of ovarian hyperstimulation syndrome].

Ovarian hyperstimulationsyndrome (OHSS) is a serious and potentially life-threatening complication of ovarian stimulation. The clinical picture of severe OHSS, includes ovarian enlargement, ascites, pleural effusion, hypercoagulation and electrolyte disorders. In this study we describe some factors which are linked with pathogenesis of OHSS. We had a case of moderate OHSS with a twin pregnancy after IVF-ET. In this case, because of abdominal pain and discomfort from the ascites, we perform abdominal paracentesis without termination of pregnancy.

Ascites↗
PubMed2002

Congenital toxoplasmosis: eye manifestations in infants and children.

The eye manifestations of congenital toxoplasmosis were investigated in 38 infants and children. Diagnosis of the congenital ocular lesions was established clinically, using also X-ray, computed tomography and ultrasonography, and confirmed by two immunodiagnostic techniques. Infants and children were examined when one or more characteristic manifestations of ophthalmic disorders were present. Typical symptoms and signs can present at birth or appear later in life. The multiplicity of clinical manifestations is characteristic of the disease. The most common symptom was chorioretinitis (92%), associated in 71% of the cases with other ocular lesions, and the second most common symptom was microphthalmia and strabismus. The inflammatory process also involved the anterior segment of the eye: iridocyclitis, cataract, glaucoma. Other rare findings were hydrocephalus, calcification in the brain, paresis, epilepsy and diminished visual acuity. The study showed that transplacental transmission of Toxoplasma to the fetus can have severe clinical consequences and terminate in fatal ocular impairment.

Adolescent↗
PubMed2000

[The basic problems and outlook for assisted reproductive technologies].

In this article the authors discuss some problems in ART:COH, stimulation protocols, culture media, transfer of embryos, luteal phase support, ethical, financial, religious and legal problems etc. Tha main complications are discussed, toosyndrom and pseudosyndrom of the empty follicle, OHSS, multiple pregnancy. In these cases the prophylactic and therapeutical measures are pointed out. Finally the main perspectives and developments of ART in the new millennium are outlined.

Ethics, Medical↗
PubMed1998

Rearrangement of T-cell receptor and immunoglobulin heavy chain genes in leukemia and lymphoma.

The development of polymerase chain reaction /PCR/ has greatly facilitated the detailed characterization of T-cell receptor /TcR/ and immunoglobulin heavy chain /IgH/ gene rearrangements. The antigen-receptor genes in lymphoid cells undergo rearrangements by combination of variable /V/, diversity /D/ and joining /J/ gene segments, deletion of germline nucleotides and insertion of nontemplate-derived nucleotides /"N-regions"/. In this preliminary study we analysed 12 DNA samples from patients with lymphoproliferative disorders /LPD/ and with acute nonlymphoblastic leukemias /ANLL/. PCR analyses were performed using V and J consensus primers for conserved regions of TcRg and IgH genes. PCR products were evaluated by polyacrilamide gel electrophoresis and denaturing gradient gel electrophoresis. Applying this method, we have detected 3 TcRg and 4 IgH monoclonal rearrangements in all 12 LPD samples. One ANLL sample showed monoclonal rearrangement for TcRg. Because of the clonal rearrangements, the analysis of TcR and IgH gene configuration can be used to track the persistence of malignant cells which are determined at diagnosis. The positive results from ANLL samples will be further evaluated as prognostic criteria.

Base Sequence↗
PubMed1998

LDL-receptor gene polymorphism in patients with early-onset coronary artery disease.

Simultaneous amplification of the promoter and 17 (out of 18) exons of the LDL-R gene (along with flanking intron sequences) was performed in 36 patients with hypercholesterolemia and early-onset coronary artery disease. The identification of the PCR-products on denaturing gradient gel electrophoresis (DGGE) revealed 34 cases of heteroduplexes in 24 patients. The heteroduplexes in exons 12, 10, and 2 (31%, 28% and 22%, respectively) probably reflect common polymorphism of LDL-R gene, while heteroduplexes in exons 6, 4 and 13 are less frequent (5.6%, 5.6%, and 2.8%, respectively). Significant association between the genetic variants, or polymorphism with clinical manifestations, could not be established.

Adult↗
PubMed1998

A case of Friedreich's ataxia confirmed by DNA-analysis.

The first case of DNA-confirmed Friedreich's ataxia in Bulgaria is presented. The results from the DNA studies of the index patient revealed two alleles with an expansion between 500 and 1500 repeats of the GAA trinucleotide in the first intron of the X25 gene. The parents had one normal allele with 7-22 repeats and one allele with a similar expansion to that of the patient in the first intron of the X25 gene. These results confirm the homozygous mode of transmission of the abnormal alleles (with an expansion of the GAA trinucleotide in the first intron of the X25 gene) from the two normal heterozygous parents to their affected offspring.

Adolescent↗
PubMed1998

Quantitative analysis of the expression of apoptosis-related genes.

BACKGROUND: The development and subsequent introduction of current molecular biology techniques allows the elucidation of some of the mechanisms of the programmed cell death (apoptosis). The overexpression of the genes directly (B-cell leukemia/lymphoma-2, BCL-2) or indirectly (multidrug resistance gene-1, MDR1) involved in the suppression of apoptosis, as well as the low-level expression of their antagonists (BCL-2 associated X-protein, BAX) result in longer survival and the development of drug resistance in malignant cells. AIM: The objective of this study was to design our own appropriate primers and to synthesize internal RNA standards and this way to evaluate the rate of expression of the MDR1, BCL-2 and BAX genes using a competitive reverse transcription and polymerase chain reaction (cRT-PCR). MATERIAL AND METHODS: Peripheral blood or bone marrow aspirates from 5 patients and 3 healthy controls were used for RNA extraction. Competitive RT-PCR was employed utilizing the constructed specific primers and synthesized internal RNA standards. Following electrophoresis, the PCR products stained with ethidium bromide were evaluated on the "Gel Doc 1000TM" video image system. RESULTS: The constructed primers specific for each of the three genes and synthesized internal RNA standards with a suitable deletion allow receiving the yield of well-separated competitive amplicons. The measured band's intensity of light and the calculated standard/patient ratio were employed in the analysis of the MDR1, BCL-2 and BAX gene expression. CONCLUSIONS: Competitive RT-PCR is a non-radioactive and comparatively rapid molecular biology method suitable for everyday laboratory practice. The possibility for simultaneous evaluation of the expression of several genes could help in the elucidation of some of the mechanisms underlying the prolonged survival of malignant cells.

Apoptosis↗
PubMed1997

Evaluation of an ion-selective electrolyte analyzer: Microlyte 6.

Microlyte 6 (Kone, Finland) is an ion-selective analyzer designed to measure simultaneously the concentration of six important electrolyte parameters--potassium, sodium, chloride, ionized calcium, ionized magnesium and pH in whole blood, serum and plasma. Two values are obtained in analyzing the ionized fractions of magnesium and calcium--one at the actual pH and another at a recalculated measurement for pH = 7.4. Direct determination of ionized calcium and ionized magnesium simultaneously with that of the other electrolytes is of great clinical significance. It is only recently that ion-selective analysis of ionized magnesium has been proposed. The analytical reliability of the results and the operational characteristics of the Microlyte 6 ion-selective analyzer were evaluated for approximately one year. The coefficient of variation of the results in the reference and pathological range was 0.49%-2.23%, and 0.68%-4.42% for the within-run and between-run series, respectively. The inaccuracy of the results expressed by d% was from -4.23% to +4.06%. The comparative evaluation of the results for potassium, sodium, chloride, and ionized calcium between Microlyte-6 and the clinical chemistry analyzer Dynamic (Kone) showed a high correlation (correlation coefficient in the range 0.9868-0.9970). The correlation between the results for the ionized fraction and those obtained for total magnesium was consistent with that generally given in the literature.

Chemistry, Clinical↗
PubMed1994

[Endometriosis in clinical practice].

The authors are investigated the frequency of the cases of genital endometriosis, its most frequent localization and the choice of treatment on 864 women patients who have undergone operations connected with other gynaecological disorders and on 32 women patients, suffering sterility and proved endometriosis. Most frequent are the cases of combination between uterine myoma and endometriosis of the uterine wall-5.74% and with the patients who have undergone surgery for malignant disease between endometrium carcinoma and uterine endometriosis. The conservative treatment is the method of choice with the infertile women suffering a slight stage of endometriosis and the least conservative method is the surgical treatment, followed by a hormonal treatment resulting in the increase of the fertility of women who have the average stage of development of endometriosis.

Adult↗
PubMed1992

Studies on the plasma cholesterol and triacylglycerols in chronic methemoglobinemia in rats.

Plasma cholesterol and triacylglycerols were measured in rats with modelled chronic two-stage (mild and moderate) intermittent nitrite methemoglobinemia for 15 and 30 days. It was found that at the moment of methemoglobinemic peak (60 +/- 10 min) the experimental animals had mixed (hemtoxic, anemic and hypoxic) hypoxemia. The every day "pulse" decrease of the total oxygen concentration during the 30-day methemoglobinemia was accompanied with a significant rise (p < 0.05) of cholesterol concentrations in the high-density lipoproteins and the total cholesterol, as well as a decrease in the amount of triacylglycerols. These changes are considered to represent the side effects of adaptation for whose elucidation further research is needed.

Animals↗