Biomedical subjects

V M Zemskov

Publications and source records attributed to V M Zemskov.

At least 73 records · Page 4Linked to original sources

PubMed1981

[Histochemical study of sodium nucleinate-activated peritoneal macrophages].

The activity of various enzyme groups and the levels of protein and glycogen in the peritoneal macrophages of CBA mice treated intraperitoneally with sodium nucleinate were determined histochemically with the use of cytophotometry. A significant increase in the activity of the lysosomal enzymes, such as acid phosphatase and nonspecific esterases and an increase in the activity of lactate, malate, glutamate, alpha-glycerophosphate, and beta-hydroxybutyrate dehydrogenases. NAD.H- and NADF.H-diaphorases accompanied by an increase in the level of the total protein and glycogen were observed. Analysis of the changes was indicative of an increase in the inherent physiological metabolic and functional level under the effect of sodium nucleinate. It is suggested that the macrophage sub-populations with an increased metabolic activity appeared in the peritoneal exudate of the stimulated animals. Participation of the host low molecular DNA in the maintenance of the physiological level of th nonspecific antiinfectious resistance is suggested.

Animals↗

PubMed1980

[Combination of antibiotics and sodium nucleinate in the therapy of an experimental mixed infection due to pyogenic bacteria].

Mice were infected wtih a mixed culture of pathogenic Staphylococcus and Pseudomonas aeruginosa. The doses of sodium nucleinate were titrated. When used for prophylactic and treatment-prophylactic purposes, these doses did not change the antiinfection resistance of the animals. The doses of tetracycline and lincomycin combination (lincotetrin) having no therapeutic effect on repeated use of the combination were also chosen. It was shown that the combined use of the antibiotics and sodium nucleinate in the above doses promoted a significant increase in the animal survival rate while the drugs used alone did not promote any increase in the survival of the mice. The decrease in the death rate of the animals was observed both with the parenteral and the oral use of sodium nucleinate.

Animals↗

PubMed1979

[Intensification of antibacterial and antiviral nonsusceptibility and of the immune response with an officinal RNA preparation].

Sodium nucleinate increased essentially the insusceptibility of mice to pathogenic escherichia, strain O26, Pr. vulgaris, Ps. aeruginosa, Ser. marcescens, and produced a total stimulating effect on the nonspecific bacterial resistance; analogous stimulating activity was found in the homologous low polymeric RNA from the liver. Sodium nucleinate intensified the insusceptibility of the animals to the tick-born encephalitis and encephalomyelitis viruses, and increased the antibody-forming cells count. The side-effect of heat-inactivated vaccine from pathogenic escherichia was reduced in animals inoculated with sodium nucleinate preliminarily.

Animals↗

PubMed1979

[New data on the tachyphylactic properties of sodium nucleinate].

Sodium nucleinate, when injected into mice in combination with killed E. coli or prodigiozan, caused a decrease in side effects produced by the vaccine or polysaccharide. The combined injection of sodium nucleinate and prodigiozan in doses, ineffective if introduced separately, was accompanied by the potentiation of their tachyphylactic action. The use of sodium nucleinate in combination with polyvinylprrolidone (hemodez) prolonged the tachyphylactic action of sodium nucleinate and increased its effectiveness. The proposed principle is supposed to be suitable for decreasing the reactogenicity of bacterial vaccines and polysaccharides.

Animals↗

PubMed1978

[Aggravation of experimental dysentery in mice and acceleration of the process of multiplication of pathogenic and conditionally pathogenic bacteria under the effect of yeast RNA].

Simultaneous infection of mice with Sh. flexneri subtype 2a, culture, with various doses of RNA sodium salt (NaRNA) or administration of the preparation 3 hours prior to or 6, 12, and 18 hours after the infection was accompanied by aggravation of the experimental infection in mice. This was expressed in a greater number of animals which contracted the disease and died, and in increased blood and peritoneal exudate microbiol density. The same processes were noted in mice infected with equal doses of shigella cultures passaged four times through broth with NaRNA, in comparison with the animals infected with shigellae passaged through broth alone. Incubation of a number of pathogenic and conditionally pathogenic microbes in broth with NaRNA was accompanied by a significant increase in the bacterial biomass volume; at the same time other conditionally patoogenic microbes or even different strains of the same type of the microbes reacted to NaRNA but weakly. A possibility of realization of all of these processes in vivo and their influence on the origination of bacterial complications and development of the infection is supposed.

Animals↗

PubMed1978

[Sodium nucleinate increase of nonspecific macroorganism resistance to conditionally pathogenic and pathogenic microorganisms].

Sodium nucleinate significantly increased the non-specific resistance of mice to E. coli O26, Ps. vulgaris, Ser. marcesens, Ps. aeruginosa, Kl. ozaenae and their associations and total resistance also accompanied by a significant decrease in the number of the bacteria in the spleen and blood, the total number of the cells in the peritoneal exudate and the number of the cells adhering to the glass (macrophages). The preparation is a low molecular RNA consisting mainly of fraction 3S and a small amount of fraction 4S. It contains 1.5 per cent of protein and 2 per cent of DNA and does not contain any polysaccharides. Repeated purification of sodium nucleinate lowering the levels of the admixtures 5 times did not change its efficacy. The low molecular RNA of the rat liver (4S) had a pronounced stimulating activity. On infection of the host stimulated with sodium nucleinate, formation of the post-infection immunity was not decreased.

Animals↗

PubMed1977

Comparison of interferon-inducing activities and antiviral effects of tobacco mosaic virus, tilorone and sodium nucleinate.

Endogenous interferon was produced in animals in response to the administration of tobacco mosaic virus (TMV), tilorone and sodium nucleinate. The relationship between interferon production and the kind of inducer and the route of its administration was studied. TMV was completely innocuous for Macaca rhesus monkeys and mice and caused no untoward effects in humans upon peroral administration. TMV, tilorone and sodium nucleinate given per os exerted a marked protective effect in mice against tick-borne encephalitis, eastern and western equine encephalomyelitis and influenza virus infections.

Administration, Oral↗

PubMed1977

[Increasing the non-specific resistance of animals to pathogenic E. coli with preparations of RNA].

Yeast RNA and sodium nucleinate considerably increased the nonsusceptibility of mice to pathogenic escherichia and typhoid salmonellae. Ineffective single nucleonate doses created an intense resistance in repeated use; prolonged application was not accompanied by the appearance of tolerance to the preparation. The principal mechanism of the induced phenomenon consisted in the intensification of the bacterial elimination and the endotoxin detoxification chiefly realized by the activity of the mobile phagocytes.

Animals↗

PubMed1977

RNA-induced intensification of antibacterial resistance and aggravation of infection.

The injection of yeast total RNA, transfer RNA or sodium nucleinate into the organism of experimental animals induced the development of non-specific resistance to pathogenic salmonellae, staphylococci and escherichiae 4-6 hours after the administration of the preparations; this resistance persisted during several days. The content of serum lysozyme rose and interferon was induced in the stimulated animals, but the state of resistance was not transferred with serum. It was due to mobile phagocytes the number of which increased considerably and so did their digestive activity. Simultaneous administration of RNA and pathogenic salmonellae and staphylococci was accompanied by aggravation of infection and acceleration of the lethal outcome. The mechanism of this phenomenon consists in the acceleration of proliferation of the microorganisms and in the selection of their virulent clones under the effect of RNA which was demonstrated in experiments in vivo and in vitro. It is believed that endogenous nucleic acids participate in the development of the natural infectious process, in bacterial complications of viral infections and in autoinfection during radiation sickness.

Animals↗

PubMed1976

[Interferonogenic and antiviral activity of the tobacco mosaic virus, tilorone and sodium nucleinate].

Production of endogenic interferon in animals in responce to administration of tobaco mozaic virus, tilorone and sodium nucleinate was shown. Dependence of interferon production on the type of the inductor and the route of its administration was studied. Absolute innocuiuty of the tobaco mozaic virus for monkeys (macaco-resus) and mice, as well as the absence of any side effects in humans treated with it perorally was shown. The tobaco mozaic virus, tilorone and sodium nucleinate used perorally in treatment of experimental infections of mice caused by the viruses of East and West encephalomyelitis, influenza and tick encephalitis had a pronounced protective effect.

Animals↗

PubMed1975

[Increasing the non-specific resistance of animals to staphylococcus with official RNA preparations].

A single or double parenteral injection to mice of sodium nucleinate considerably elevated their nonspecific resistance to the virulent staphylococcus as soon as in four hours; the resistance remained marked for four days. Daily prolonged injection of the stimulant also proved to be very effective. In the simulated organism there occurred a significant depression of reproduction of the virulent staphylococci and an increase in the amount of phagocytic cells and their digestive activity. The latter served as a material basis for induced nonspecific resistance. The data obtained substantiated a possibility of using sodium nucleinate as an antibacterial nonspecific stimulant under clinical conditions.

Animals↗

PubMed1974