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Biomedical subjects

V M Zemskov

Publications and source records attributed to V M Zemskov.

At least 55 records · Page 3Linked to original sources
PubMed1985

[Structural and functional analysis of the respiratory burst in the phagocytosing macrophage].

The interrelation between structural changes and oxygen consumption by the phagocyting macrophage was studied. The mean number of phagocyted particles was estimated by the method of stereological transformation. It is found that the uptake of yeast particles and CN- -nonsensitive oxygen consumption is related to the concentration of yeast cells in the incubation medium. A positive correlation was established between the oxygen consumption and the mean number of phagocyted particles. The results obtained may suggest that the "respiration burst" takes place in the contact area of the macrophage and the phagocyted material, and its extent probably depends on the surface of that contact area.

Animals↗
PubMed1985

[Redistribution and activation of peritoneal macrophages with the peroral use of sodium nucleinate].

Three hours after the administration of sodium nucleinate the activation of glycolysis, the hexosomonophosphate shunt, the urea cycle (determined by the analysis of key enzymes), the expression of FC gamma-receptors and the decrease of oxidizing phosphorylation were noted in the peritoneal macrophages of mice. By 36 hours the gradual decrease of these characteristics occurred. After three oral administrations of the preparation immature macrophages with lower metabolic activity and increased oxidizing phosphorylation appeared in the exudate, while resident and activated cells practically disappeared. The immaturity of the cells was confirmed by their incubation in vitro. The changes in the cells, revealed in this investigation, are supposed to occur due to the redistribution of activated and intact macrophage populations in situ.

Adjuvants, Immunologic↗
PubMed1984

Visualization of reactive oxygen species formation by phagocytizing macrophages.

Activated peritoneal macrophages exhibiting phagocytosing capacity produced an electron-dense precipitate of formazan in contact sites of macrophage plasmalemma and phagocytosed yeast cells. No production of formazan occurred, when non-opsonized latex particles were ingested by macrophages. Formazan precipitation could be prevented by anaerobiosis but not by addition of cyanide.

Anaerobiosis↗
PubMed1984

[Comparative effectiveness of immunomodulators in the therapy of rheumatic heart defects].

A total of 166 patients with rheumatic heart diseases were examined. It was shown that the patients had secondary immune deficiency mainly with respect to the T-cells, which could not be eliminated with the routine therapeutic agents. The use of hemodes, decaris or sodium nucleinate in the combined therapy of such patients promoted correction of the immune deficiency and increased the treatment efficacy. Sodium nucleinate proved to be the most active.

Adjuvants, Immunologic↗
PubMed1984

[Correction of secondary immunologic deficiency by using yeast RNA].

In patients with rheumatism, chronic alcoholism and peptic ulcer immune deficiency mainly with respect to T-lymphocytes has been detected. The injection of sodium nucleinate simultaneously with the use of traditional therapeutic remedies induces the correction of secondary immune deficiency for a period of 6-9 months.

Adjuvants, Immunologic↗
PubMed1984

[Principles for assessing and correcting secondary immunological deficiency].

Examination of 923 patients with bronchopulmonary diseases, rheumatic fever, peptic ulcer, hypertension and chronic alcoholism revealed the presence of immune deficiency, particularly as regards the T immunity system. Inclusion of sodium nucleinate into multiple modality treatment promoted correction of immune deficiency and remission of the underlying disease. The patients presenting with peptic ulcer showed the correlation between blood serum RNA and immunological characteristics.

Adjuvants, Immunologic↗
PubMed1984

[Structural and functional study of acidification in the process of phagocytosis].

Morphometry and cytofluorometry of the kinetics of phagocytosis were applied to study the quantitative mechanisms of acidification in the area of contact between the plasma membrane of macrophages and Candida albicans yeast cells conjugated with fluorescein isothiocyanate. It was found by stereological transformation of the morphometry data that the main part of macrophages phagocytose the limiting amount of particles by minute 5-10. A good agreement was established between the cytofluorometric histograms and the stereological data. This made it possible to evaluate, using the calibration curve, the pH on the surface of the phagocytosed material based on the fluorescence quenching. As an advantage of the suggested comprehensive approach to the study of acidification, the authors stress the possibility of making structural-functional analysis of the rearrangements occurring in the intact phagocytosing cell.

Animals↗
PubMed1984

[Comparative effectiveness of immunomodulators in the therapy of bronchopulmonary diseases].

One hundred and fifty-one patients with bronchopulmonary diseases were examined and it was shown that they had secondary immune deficiency mainly in the T-cells which could not be recovered with the routine therapeutic agents. The use of hemodez, levamisole or sodium nucleinate in the combined therapy promoted correction of the immune deficiency and increased the treatment efficacy. Sodium nucleinate proved to be the most efficient.

Adjuvants, Immunologic↗
PubMed1984

[A new electron microscopic method of determining acidification in phagocyte subcellular structures].

A new method is elaborated for detecting acidification in phagocytes on the ultrastructural level. The method is based on the reaction between Cu2+ and [Fe(CN)6]4- which form a pellet of cupric ferrocyanide in the neutral medium. It is possible to induce pellet formation under definitely determined pH values on adding different amounts of chelating agent (citrate) to the reaction mixture. The fine-grained electron dense pellet of cupric ferrocyanide persists throughout the whole subsequent procedure of fixation, dehydration and embedding of the biological material for electron microscopy. Data are presented on the degree of acidification and its localization in subcellular structures of the phagocyte during phagocytosis.

Animals↗
PubMed1983

[Acidification kinetics in macrophage phagosomes based on flow cytometric data].

Using flow cytofluorimetry, acidification in macrophages was studied during phagocytosis of yeast cells Candida albicans conjugated with FITC. Two phases of acidification were found: one corresponding presumably to yeast cell absorption on the macrophage surface, and the other one being due to cell incorporation into phagolysosomes. The phases are characterized by pH values of 6.3 +/- 0.5 and 4.5 +/- 0.5, respectively. Addition of 10 mM NH4Cl cancelled the observed acidification, whereas the presence of cytochalasin B (2 . 10(-5) M) prevented the very process of acidification.

Animals↗
PubMed1983

[Electron microscopic-cytochemical method of determining cell phagolysosomal structures].

A new electron-cytochemical method for detection of phagolysosomal structures in intact cells is proposed, which is more susceptible and simple in comparison with methods described elsewhere. The new method involves the addition of sodium beta-glycerophosphate and Pb(NO3)2 to the medium where phagocytes are incubated with phagocytic material. After the fusion of a phagosome containing the phagocytic material, sodium beta-glycerophosphate (the substrate for a marker lysosomal enzyme--acid phosphatase) and Pb2+ with primary lysosomes, hydrolysis of this substrate occurs with the eventual appearance of a deposit of Pb3(PO4)2.

Animals↗
PubMed1982

[RNA as a modulator of secondary immunological deficiency and dysnucleotidosis].

Secondary immunological deficiency was identified in patients with chronic pneumonia, rheumatism, peptic ulcer or chronic alcoholism. The routine therapy resulted in aggravation of the immunity defects in the patients with pulmonary affections and chronic alcoholism. The combined therapy with the use of sodium nucleinate promoted elimination of the immunological deficiency, whereas in the patients with peptic ulcer, elimination of the increased content of 17-HKS in the urine and dysnucleotidose.

Adult↗