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V M Getsova

Publications and source records attributed to V M Getsova.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Modulation of the activity of monoaminergic brain systems and emotional condition by dalargin in rats during development of emotional resonance response].

Rats were divided in two groups by the reaction of emotional resonance (RER): with emotionally positive reactions (I, with fast RER acquisition, up to 100 s) and with emotionally negative reactions (II, with slow RER acquisition, more than 200 s). After the RER acquisition, the activity of 5-hydroxitryptophan (5-HT) system of the I group of animals was lower than in the II group. The activity of noradrenaline (NA) and dopamine (DA) systems of the I group of animals was higher than in the II group. The between-group differences were enhanced by subcutaneous injection of dalargin. In some brain structures dalargin reversed these relations. These findings point to a complicated interpenetrating character of emotionally positive and emotionally negative states. Emotionally positive states include components of emotionally positive states, and emotionally negative states include components of emotionally positive states. Increase in 5-HT activity and decrease in activity of NA, DA, and opioid (OP) systems induce formation of emotionally negative states. Decrease in 5-HT activity and increase in activity of NA, DA, and OP systems induce formation of the emotionally positive state. It is suggested that 5-HT, NA, and DA systems play the central role in the processes of reinforcement, acquire the evaluative function, and are included in realization of all types of behaviors. OP is a primary modulator system which accompanies the unconditioned pain stimulus and connects it with systems of evaluative function.

Animals↗
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[Possible mechanisms for forming typological features of behavior of WAG/Rij line rats].

Typological behavior reactions of WAG/Rij rats were studied from the standpoint of divergent modulatory integration hypothesis. This rat strain has a genetically determined dominant dysfunction of the benzodiazepine system of the thalamic nuclei. This disorder provokes an epileptiform disease such as absence epilepsy. It was suggested that the dysfunction of this system would result in a modification of the modulatory systems, which support the motivation states of escape and avoidance reactions as well as of the modulatory systems, which form the emotional states. Modifications of these states are the background of typological behavioral features of WAG/Rij rats. It was shown that WAG/Rij have the lower threshold of the development of haloperidol catalepsy, higher levels of fear and depression. On the first day of training in a shuttle box, WAG/Rij rats demonstrated better avoidance performance than Wistar rats. On the second and 28th days, the amnestic effect of the epileptiform disease was observed. The amnestic effect was also observed after passive avoidance conditioning. The results are discussed in terms of the modulatory integratin hypothesis.

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PubMedDate not supplied

[The effect of monoamine oxidase inhibition on formation, fixation and reproduction of temporary connections].

An administration of 100 to 300 mg/kg doses of iprazid to rats and mice leads in 24 hours to a considerably higher serotonin and noradrenaline content in the brain. Defensive conditioning against this background proceeds in the same way as in control animals but the retention of elaborated reflexes is sharply disturbed. A similar effect is produced by 5-oxytryptophane. An iprazid administration after conditioning does not disturb the retention and subsequent achievement of the reflex. Inclusion of S35-methionine in the total proteins of the mice brain does not change under the influence of iprazid. A conclusion has been drawn that inhibition of monoaminoxidase by iprazid does not prevent the formation and reproduction of temporary connections, but deeply disturbs their fixation. It is suggested that the revealed disturbances of fixation of temporary connections are due to the accumulation of serotonin in the brain, which suppresses the protein synthesis in the synaptosomes or that of specific proteins.

Animals↗
PubMedDate not supplied

[Low dose of disulfiram and l-dopa evoked synergistic modification in behavior of Wistar and WAG/Rij rats].

Comparative analysis of behaviors of two rat strains, Wistar and WAG/Rij, was performed. No behavioral differences between Wistar and WAG/Rij were found in the emotional resonance test. Disulfiram injection produced similar effects in both rat strains. Animals of the first group (with slow acquisition of emotional resonance reaction) transformed into the animals of the second group (with fast acquisition). Passive avoidance conditioning was successfully reproduced in Wistar and was significantly impaired in WAG/Rij. A low dose of disulfiram injected before or immediately after conditioning substantially improved the reproduction to a greater extent in WAG/Rij than Wistar strains thus eliminating in interstrain differences. Active avoidance conditioning was more successful in WAG/Rij than in Wistar rats However, on the next day conditioning in WAG/Rij was substantially impaired. Administration of the low dose of disulfiram or L-DOPA prior to conditioning impaired the acquisition but improved the reproduction on the following day in both strains, but disulfiram injection after conditioning improved conditioning in WAG/Rij to a greater extent than in Wistar. Thus, the pharmacologic enhancement of the reward system substantially changed animal behavior and improved memory consolidation.

Affect↗
PubMedDate not supplied

[Effect of a decrease in the concentration of serotonin in the brain on elaboration and preservation of defensive conditioned reflexes].

Injection of a total dose of 1200 mg/kg of parachlorphenylalanine to mice leads to a 75% drop in the serotonin (5-OT) content in the brain with a subsequent return to the initial level in seven days. The processes of elaboration and reproduction of conditioned defensive active and "passive" avoidance reflexes were studied against the background of reduced 5-OT content. It has been assumed that the observed disturbances in elaboration are linked to the increased fear emotion when the mice are placed in new surroundings. Disturbances in retention of the "passive" avoidance conditioned reflex are in fact disturbances of its reproduction. A conclusion has been drawn that a drop in the 5-OT content in the brain does not prevent the formation and fixation of defensive temporary connections.

Animals↗
PubMedDate not supplied

[Effect of serotonin on processes of elaboration and fixation of heterogeneous temporary connections].

The effect of serotonin (5-OT) level in the brain on the elaboration and retention of heterogenous conditioned reflexes was studied in the experiments on rats and mice. Redundancy of 5-OT deteriorated the retention of defensive and alimentary conditioned reflexes and almost fully prevented the fixation of drinking conditioned reflexes. The reduction of 5-OT led to less pronounced disorders in the retention of conditioned reflexes. With a prolonged delay of electrocutaneous reinforcement (from 5 to 10 s) the control animals showed slower elaboration of two-side avoidance reflexes, while their retention was uneffected. The redundancy of 5-OT in these conditions accelerated the elaboration but at the same time deteriorated the retention as in case of 5-second delay. A conclusion is made that with the changed level of 5-OT the impairment of conditioned reflexes retention doesn't depend on the changes in the speed of their elaboration. The "point of application" of changes in 5-OT content lies in the process of the temporary connection consolidation. The degree of 5-OT participation in these processes depends on the characteristics of elaborated conditioned reflexes.

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PubMedDate not supplied

[Effect of a decrease in brain noradrenaline and serotonin levels on defensive and food-getting conditioned reflexes in rats].

The influence of pharmacological and surgical interferences in the activity of serotoninergic and catecholaminergic brain systems on elaboration and consolidation of conditioned reflexes was studied in rats. Ablation of the locus coeruleus, nucleus raphe and administration of inhibitors of monoamines synthesis reduced noradrenaline and serotonin (5-OT) content in rat's brain. Ablation of the locus coeruleus and disulphiram administration impaired mainly the elaboration of defensive and alimentary conditioned reflexes. Ablation of the nucleus raphe and parachlorphenilalanin administration affected predominantly the retention of the reflexes. At the same time, the effects of surgical and pharmacological interferences differed in some respects

Animals↗
PubMedDate not supplied

[The noradrenaline and serotonin content in symmetrical parts of the normal rat brain and during learning and peptide administration].

Content was compared of noradrenaline (NA) and serotonine (5-OT) in the right and left halves of the rats brain in norm, at elaboration of defensive conditioned reflexes of two-ways avoidance (CRTWA) and at administration of neuropeptides influencing the learning and memory--dezglycilargininvasopressin (DG-AVP), ACTH4-7 pro-gli-pro and dalargin. The conducted studies showed that in control animals the content of NA in the cortex of the right hemisphere was significantly higher than in the cortex of the left one. For the content of 5-OT in symmetric brain parts no significant differences were revealed. Under the elaboration of CRTWA the asymmetry of NA content was not eliminated. Systemic administration of DG-AVP, ACTG4-7 pro-gli-pro and dalargin practically did not change the content of 5-OT, but reduced the content of NA in the cortex and the rest of the brain, and the content of NA in the right and left cortex was equalized. The obtained data point to the asymmetric character of neuropeptides action and to greater resistance of 5-OT-ergic brain system to functional load and to administration of peptides in comparison with NA-ergic system.

Adrenocorticotropic Hormone↗
PubMedDate not supplied

[Serotonin excess in the brain modifies the effect of beta-endorphin and dalargin on the processes of learning and memory].

In experiments on outbred female rats the influence was studied and compared of two representatives of endogenous opioids beta-endorphine and the analogue of leu-enkephalin dalargin on the processes of learning and memory in normal conditions and at the change of functional state of serotoninergic system of the brain. Parallel, the influence was studied of neuropeptides on the content of serotonin (5-OT) and its metabolite--5-oxyindolacetic acid in various areas of the brain in control and at the 5-OT redundancy. Conditioned reflexes (CRs) were used of two-way avoidance and defensive CRs. It has been established that administration of neuropeptides to intact animals influences in different directions the elaboration of the CR of two-way avoidance and maze defensive CR, but also worsens their preservation. Redundancy of 5-OT in the brain modifies behavioural effects of beta-endorphine and dalargin manifested in appearance of new effect and elimination and change of direction of the effects observed in the intact animals. Redundancy of 5-OT in the brain changes metabolic effects of beta-endorphine and particularly of dalargin. The obtained data testify to a dependence of the effects of beta-endorphine and dalargin on the functional state of 5-OT-ergic system.

Animals↗
PubMedDate not supplied

[Effect an ACTH analog on the processes of learning and memory in rats].

On the basis of the idea of the important role of neurotransmitter systems in realization of neuropeptide effects, the participation was studied of the monoaminergic systems in the mechanisms of the ACTH analogue influence on the processes of learning and memory in control animals and animals with a changed functional state of the monoaminergic systems. In parallel the influence was studied of the ACTH analogue on the content of the endogenic monoamines in various brain structures of rats. It has been shown that administration of the ACTH analogue in a dose of 10 mcg affects the elaboration and preservation of conditioned reflexes (CRs) of passive avoidance, CRs of two-side avoidance and labyrinth CRs only in conditions of changed functional state of the monoaminergic systems. Amnesia, usually elicited by 5-oxytryptophane and disulfiram is prevented by administration of the ACTH analogue. Administration of the ACTH analogue is accompanied by the intensification of serotonine metabolism in the midbrain and medulla and by an increase of noradrenaline content in the hypothlamus.

5-Hydroxytryptophan↗
PubMedDate not supplied

[Effect of a cyclic analog of enkephalin on learning and memory in the mouse].

In experiments on mice, the effect of cyclic analogue of enkephalin (CAE) on the processes of learning and memory was studied in control animals and in animals with changed functional state of monoaminergic brain systems. Administration of the peptide to intact animals significantly accelerated the acquisition of conditioned reflexes of two-way avoidance and did not significantly affect the retention of these reflexes and their subsequent reproduction. Retention and reproduction of conditioned reflexes elaborated in one combination, was disturbed. Administration of iprazid did not eliminate the "accelerating effect" of CAE on the formation of conditioned reflexes of the two-way avoidance but sharply disturbed their retention. In such conditions, the amnesing iprazid effect increased still more. Besides, under CAE effect, the activity of acetylcholinesterase in the motor and especially in the visual cortex of the mice increased. The obtained data testify to an important role of the monoaminergic and cholinergic brain mechanisms in realization of CAE effects on the processes of learning and memory.

Acetylcholinesterase↗
PubMedDate not supplied

[Effect of dalargin on the processes of memory and learning in the rat].

In experiments on rats, the influence was studied of dalargin on the elaboration and preservation of various homogeneous and heterogeneous conditioned reflexes (CRs) elaborated in single and multiple pairings. The effect of dalargin on the processes of learning and memory was compared with the action of the peptide on the activity of hypothalamic neurones. Administration of dalargin delayed the elaboration of maze defensive CRs and practically did not affect the elaboration of two-way avoidance. The preservation of CR also deteriorated under the influence of dalargin. Administration of dalargin 10 min before the CRs testing did not prevent their reproduction. When using CRs elaborated in a single pairing, dalargin disturbed the preservation of the drinking CR and improved that of passive avoidance CR. Dalargin in this dose affected the emotional state of animals in the open field and did not significantly affect their motor activity. Dalargin suppressed impulse activity in 17 out of 22 tested neurones of the lateral hypothalamus, with maximum effect in 20-50 min after its administration. The obtained data show that the character of dalargin action on the elaboration of CR and mainly on its consolidation, depends on the character of the elaborated CR and is probably due to great extent to the effect of the peptide on the brain emotional mechanisms.

Animals↗
PubMedDate not supplied

[Effect of scopolamine on formation and fixation of temporary connections in rats with altered brain serotonin levels].

The influence of scopolamine on elaboration and maintenance of conditioned reflexes of two-way avoidance was studied in rats under conditions of excess and deficit of serotonin in the brain. Administration of scopolamine to intact rats accelerated conditioning and did not prevent fixation of the reflex. Administration of scopolamine to animals with a lowered level of serotonin in the brain (by means of para-chlorophenylalanine) impaired conditioning and induced amnesia. In animals with ablated raphe nuclei, the same dose of scopolamine did not prevent elaboration and maintenance of conditioned reflexes. Accumulation of scopolamine in the brain by means of 5-oxytryptophan abolished acceleration of conditioning, which is specific for scopolamine, and affected the preservation of the reflexes. Against the background of the action of iprozid, scopolamine impaired the conditioning and tended to deteriorate the maintenance of conditioned reflexes. It is assumed that the serotoninergic system exerts a modulating influence on the activity of the brain cholinergic and cholinoreactive mechanisms.

5-Hydroxytryptophan↗
PubMedDate not supplied

[Mechanisms of serotonin participation in the consolidation of temporary connections].

The effect of serotonin (5-OT) surplus on incorporation of labelled 14C-leucine and 3H-fucose in the hippocampal proteins was studied in experiments in vivo and in vitro. It was found that intrahippocampal administration of 5-OT in a dose of 5 mcg immediately after elaboration of defensive conditioned reflexes in V-shaped maze impaired the retention of these reflexes and lowered the incorporation of 14C-leucine in the hippocampal proteins. Doubled dose of 5-OT did not cause amnesia and did not change the protein synthesis. In experiments in vitro 5-OT decreased 14C-leucine incorporation and increased 3H-fucose incorporation in total proteins of hippocampal section. The changes in labelled leucine incorporation were in a non-linear dependence on 5-OT concentration. Similar but less pronounced changes in the protein synthesis were also found on sections of the hippocampus taken from animals with a life-time 5-OT surplus. The obtained data confirm the assumption that 5-OT participates in consolidation processes through its controlling effect on protein metabolism.

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