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Biomedical subjects

V Liso

Publications and source records attributed to V Liso.

At least 73 records · Page 4Linked to original sources
PubMed1985

Adenosine deaminase activity in peripheral lymphocytes of patients with gynaecologic malignancies.

Peripheral lymphocyte adenosine deaminase (ADA) activity was assessed in a group of 31 patients with gynaecologic malignancies (19 with carcinoma of the portio, 7 with endometrial adenocarcinoma, 3 with ovarian cancer, 1 with adenocarcinoma of the cervix, 1 with liposarcoma myxoide). 30 female subjects, aged 30 to 70 years, were studied as the control group. Lymphocyte ADA activity in the 31 patients ranged from 0 to 7 U/10(7) cells with a mean of 2.3 U/10(7) cells (normal values: range 2-5 U/10(7) cells; mean 2.8 U/10(7) cells). In cases where the enzyme was absent or far below the controls, a faster evolution of the disease was observed. We would point out that lymphocyte ADA activity in the patients under investigations shows a broad range of variability. Our preliminary observations would suggest that lymphocyte ADA assessment in a larger series of cancer patients may add further prognostic informations.

Adenosine Deaminase↗
PubMed1984

[Characteristics of the electrophoretic mobility of blasts from acute non-lymphoid leukemias].

The behaviour of electrophoretic mobility (E.M.) of the blast cells has been investigated in 15 cases of acute non lymphoid leukemias (A.N.L.L.), (eleven cases of M1 type and four M5 type). The fast and homogeneous E.M. of M5 type in comparison with M1 has been underlined. E.M. of M1 blasts has been reported in a wide range varying from a "normal" migration (similar to that of normal neutrophils and monocytes) to a "fast" one (similar to M5 type). It is suggested that M1 leukemic forms, morphologically recognizable as the same leukemic type, are different L.A.N.L. indeed.

Acute Disease↗
PubMed1983

[Prognostic usefulness of lysozyme in acute myelomonocytic leukemias].

Pre-treatment sera and urines from 17 patients with acute myelomonocytic leukemia (M-4 type) have been assayed for their lysozyme content. In addition periodical evaluations of the serum and urinary lysozyme levels have been performed during the clinical course of 10 patients. There was no correlation between initial lysozyme activity in the serum and response to chemotherapy, while periodical estimations of serum lysozyme activity, in the same patient, provide clinically significant prognostic information. Urinary lysozyme levels are closely related to serum lysozyme levels; therefore the urinary estimations of this enzyme activity provide no different prognostic evaluations than serum estimations.

Adult↗
PubMed1983

Acute lymphoblastic leukemia hand-mirror cells. Study of nine cases.

Acute lymphoblastic leukemia with hand-mirror cells (HMC) was diagnosed in nine adult patients. Blast HMC were seen only in the bone marrow (12-57% range). Cytochemical studies revealed a positive reaction to tartrate-sensitive acid phosphatase in the tail portion of the cells in seven cases, with a strong, localized cytoplasmic reaction in four. Leukemic cells lacked surface immunoglobulins and were E rosette negative in all cases. Normal levels of adenosine deaminase activity (ADA) were found in five of the seven patients. Electron microscope studies confirmed the hand-mirror shape of the cell. These HMC contained large numbers of mitochondria and microspikes in the handle portion of the cell. The patients failed to respond to initial conventional ALL chemotherapy, but the prolonged survival with passable health of the majority of these, despite their lack of complete remission, is emphasized.

Acid Phosphatase↗
PubMed1982

Platelet satellitism to basophils in a patient with chronic myelocytic leukaemia.

Platelet satellitism to basophil granulocytes was observed in a patient with chronic myelocytic leukaemia. This phenomenon occurred with peripheral basophil cells obtained from venous blood anticoagulated with EDTA. Previous reports have demonstrated platelet satellitism to polymorphonuclear neutrophils and monocytes but not to other types of white blood cells. The cause of the platelet satellitism in this CML case remains unclear. It is suggested that this rare phenomenon should be considered in evaluating the platelet number in CML.

Aged↗
PubMed1982

[Electrophoretic mobility in acute null cell lymphoblastic leukemia].

The electrophoretic mobility (E.M.) (evaluated by cytopherometer) of human normal lymphocytes, shows a group of cells with a fast mobility, identifiable as T lymphocytes, and a second group with a slow mobility which can be characterized as B lymphocytes. Six cases of Acute Lymphoblastic Leukaemia characterized by negativity of immunological markers and classified as "Null Cells", and ten cases of B cell Chronic Lymphocytic Leukaemia were studied in order to investigate the E.M. behaviour of leukaemic cells and so a comparison with T and B normal lymphocytes migration. The blasts of Acute Lymphoblastic Leukaemia show a E.M. nearly similar to the fast group of normal lymphocytes, while lymphocytes of the B cell Chronic Lymphocytic Leukaemia migrate as the slow component of normal lymphocytes. The possibility of the utilization of cell E.M. in addition to immunological and cytochemical data, into the characterization of Acute Leukaemias is suggested.

Adolescent↗
PubMed1980

The behaviour of beta-2-microglobulin in acute and chronic leukaemias.

Two hundred-one patients (97 leukaemias, 18 lymphoproliferative disorders, 86 solid tumors) were studied for B2M by radioimmune technique. Serum levels were high in all cases, excepting for ALL which presented normal values in 81% of the patients. The follow-up of the microprotein was carried out in 17 patients with acute leukaemias. The data obtained seem to suggest that subjects with high serum levels of B2M at the beginning of the disease do not respond favourably to chemotherapy.

Acute Disease↗
PubMed1978

Evaluation of non-stimulated nitroblue-tetrazolium test in patients with malignant lymphomas.

The NBT-test was performed in 54 patients with Hodgkin and non-Hodgkin lymphoma showing no bacterial infection exept for one case. 18 patients presented, at onset of the disease, a number of neutrophilic NBT-reducing granulocytes at pathological levels (greater than 12%). In order to evaluate the influence of cytostatic drugs on NBT-test serial blood samples were taken during polychemotherapy in 17 patients with lymphoma. In our experience, cytostatics (COPP) do not necessarily lessen NBT-reducing activity of granulocytes. The results suggest that the NBT in malignant lymphomas could not be used as an indication of bacterial infection.

Adolescent↗
PubMed1978

Daunomycin, cytosine arabinoside and 6-thioguanine (DAT) vs vincristine, cytosine arabinoside and 6-thioguanine (VAT) in the induction treatment of acute nonlymphocyte leukemia: a randomized collaborative study.

One hundred patients were entered in a cooperative study comparing the efficacy of two different regimens in the induction treatment of acute nonlymphocytic leukemia (ANLL). Patients were randomly allocated to receive either the DAT or VAT combination; half of the patients were also randomized to receive CNS prophylaxis including intrathecal methotrexate + prednisone and cranial irradiation. Consolidation and maintenance therapy were uniform in responding patients. Out of 82 evaluable patients 41 (50%) attained complete remission (CR) with no significant difference between the two regimens. Median remission duration was slightly longer in the DAT group (32.5 vs 22 weeks); median survival was 34 weeks for all evaluable patients with no difference between the two schedules. Meningeal relapse occurred only in two patients after 19 and 99 weeks of continuous remission. Fourteen patients are still alive after 61 to greater than or equal to 155 weeks, of whom seven are in their initial remission (six in the DAT and one in the VAT group). We conclude that 1) DAT and VAT are equally effective in inducing CR in a high proportion of ANLL patients; 2) until marrow remission can be prolonged significantly, preventing CNS leukemia will not have any significant impact of the course of ANLL.

Adolescent↗
PubMed1978

Adenosine deaminase activity in acute lymphoblastic leukaemia: cytochemical, immunological and clinical correlations.

Adenosine deaminase (ADA) activity has been assessed in lymphoid cells of 23 patients with acute lymphoblastic leukaemia (ALL) in order to attempt a further characterization of ALL cells in addition to the well known cytochemical and immunological T and B lymphoid cell markers. ADA activity did not show any correlation with the immunological characterization of the patients investigated; in fact a wide range of ADA activity was observed with levels ranging from 0 to 32 U in T-ALL patients and nearly similar values (from 1.8 to 36 U) in the group of non T--non B ALL cases. Normal values ranged from 2 to 5 U (mean 2.9 U; s.d. +/- 0.8). Some cytochemical patterns (acid phosphatase and PAS) appeared well correlated with T markers of lymphoid cells, whereas they showed no significant relationship with ADA activity.

Acid Phosphatase↗
PubMed1978

Mast cell leukemia and acute basophilic leukemia. Cytochemical studies.

Human mast cells and basophil granulocytes can be easily recognized in normal tissues by light microscopy. In one mast cell and one basophilic leukemic case considered in this study, mast cells and basophils were morphologically quite similar and could not therefore be clearly defined merely by their morphological features. Both types of cells showed round nuclei and deep purple granules. The diagnosis of mast cell leukemia or basophilic leukemia was made on the basis of different cytochemical patterns. In the case of mast cell leukemia, peroxidase and PAS stains were negative, while chloroesterase was strongly positive; in the case of basophilic leukemia, peroxidase and PAS stains were positive, while chloroesterase reaction showed a peculiar pattern. Toluidine blue metachromasia and astra blue positivity were present in the cells of both cases.

Basophils↗
PubMed1977

Cytochemical "normal" and "abnormal" eosinophils in acute leukemias.

Eosinophilic leukocytes may accompany a great variety of disorders and different types of acute leukemias. The most striking morphologic feature of eosinophils is their specific granules, but morphology alone often is insufficient to differentiate normal from abnormal eosinophils. Cytochemically, the eosinophils were considered "normal" when they did not contain alkaline phosphatase, chloroacetate esterase, toluidine blue metachromasia, Astra blue positivity, and specific PAS-positive granules, but did have peroxidase and cyanide-resistant peroxidase activities, Sudan black positivity and moderate naphthol-AS esterase or alpha-naphthyl esterase and acid phosphatase positivities. In seven cases of acute leukemias (two acute myeloblastic and five myelomonocytic), in contrast with their normal behaviour, the eosinophils show "abnormal" cytochemical positivities consisting of chloroesterase activity, PAS and Astra blue positivities of the specific granules, toluidine blue metachromasia, and cyanide-resistant peroxidase of a few specific granules. Cytochemical investigations may provide additional criteria for evaluating the abnormality of the eosinophilic cell in leukemias.

Adult↗