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Biomedical subjects

V Liso

Publications and source records attributed to V Liso.

At least 55 records · Page 3Linked to original sources
PubMed1989

Interleukin-1 and tumor necrosis factor production in acute non-lymphoid leukemia.

We have investigated interleukin-1 (IL-1) and tumor necrosis factor (TNF) release in 20 patients with acute non-lymphoid leukemia (ANLL) after culture with bacterial lipopolysaccharide (LPS) or in the absence of deliberate stimulation. IL-1 and TNF were identified by appropriate bioassays inhibitable by specific antibodies. The capacity to produce IL-1 was expressed by most ANLL cases investigated irrespective of the FAB (French, American, British) subtype. However, the M4 and M5 cases tended to be better producers of IL-1 than M1-M3 cases. In contrast, TNF release was only restricted to M5 leukemias (3 out of 4 cases examined). Cytokine production may therefore provide additional criteria for a functional classification of ANLL. A considerable proportion of ANLL cases (7/18 bone marrow samples and 12/20 blood samples) released appreciable quantities of IL-1 in culture in the absence of deliberate stimulation. "Spontaneous" TNF production was also detected in 1 out of 3 M5 cases. Cells were cultured under LPS-negative conditions and polymixin B did not affect spontaneous cytokine release. Moreover, Northern blot analysis showed that freshly isolated, non-cultured ANLL cells expressed IL-1 beta transcripts. Inasmuch as IL-1 is responsible for hemopoietin-1 activity and IL-1 induces colony stimulating factor production in various cell types, the observation of IL-1 production in ANLL suggests that this mediator may be involved in regulatory amplifying circuits of leukemic cell proliferation.

Biomarkers, Tumor↗
PubMed1989

High-dose Ara-C (HiDAC) plus asparaginase in elderly patients with acute non-lymphocytic leukemia: a pilot multicentric study by the Italian Cooperative Group GIMEMA.

A multicentric prospective pilot study using three different schedules of high-dose Ara-C at dosage of 3 g/m2 every 12 hours during 3 h of infusion was undertaken by the Italian Cooperative Group GIMEMA in order: 1. to evaluate the safety and efficacy of such treatment in previously untreated ANLL patients more than 50 years old; 2. to investigate whether the addition of a standard maintenance treatment after consolidation with 4 courses of DAT (Daunorubicin + Ara-C + 6-Thioguanine) could improve the duration of complete remission (CR) and the proportion of long-term survival. Overall 43/125 evaluable patients (34.4%) achieved CR. 32/125 died during the induction phase, the remaining 50 patients (40%) failed to achieve CR. As for the toxicity, the most significant toxicity of all schedules was hematologic. No substantial neurological or cardiac toxicity was observed. The multivariated analysis of several pretreatment characteristics revealed that age more than 60 yr, male sex and presence of infections at diagnosis were the most significant adverse factors for achievement of CR. The median duration of DFS for all responders was 9 months, with relapse-free survival at 4 yr estimated at 29%. The addition of maintenance treatment to consolidated patients had no advantages in respect to the control group, even though the statistical analysis revealed a p = 0.058. However, because of the small number of randomized patients, no conclusions can be drawn concerning the importance of maintenance treatment.

Aged↗
PubMed1989

[The phagocytic activity of the neutrophilic granulocytes via chemoluminescence and occupational exposure to lead].

The lead interference on the immunological activity is the aim of this survey. Therefore, it was examined the phagocytic function of the PMN cells on a group of 20 subjects occupationally exposed to the toxic and on 20 other assigned to the control group using the chemoluminescent assay. In both groups it was determined the biologicals parameters of exposure to the toxic. It was evident a statistically significant decrease of the average values in the exposed group compared to the control group, even if it wasn't directly correlated with the exposure biological indicators.

Adult↗
PubMed1988

Piperacillin plus amikacin versus cefotaxime plus amikacin in neutropenic and feverish patients with malignant hemopathies.

Seventy-one neutropenic patients under cytostatic treatment for malignant hemopathies (neutrophil granulocytes less than or equal to/mm3 with feverish episodes in progress (T greater than or equal to 38.5 degrees C) which were probably of an infectious nature were treated according to two antibiotic protocols (piperacillin + amikacin [P + A] or cefotaxime + amikacin [C + A] in a randomized, comparative, prospective study. Of the 71 patients enrolled, 65 could in the end be evaluated for the purposes of this study (36 treated according to the P + A protocol, 29 according to the C + A protocol). In 16 patients the infection was documented bacteriologically. In these cases the percentages of response were, respectively, 77.7% with the P + A and 71.4% with the C + A protocol. The positive clinical results of the two protocols being studied were, considering the entire survey (bacteriologically documented, clinically documented and FUO infections), respectively, 69.4% in the patients treated with P + A and 62.0% in those treated with C + A. The results of the study seem to indicate that the severity of the neutropenia (N.G. less than 500 or greater than 500) does not affect the response to the antibiotic therapy. Modest and transient side effects (hypokalemia and increase of the ClCr) were noted above all in the patients subjected to the therapy with C + A. The results of this study show, therefore, a superimposable effectiveness of the two therapeutic protocols (P + A and C + A) in the empirical treatment of infections in neutropenic patients with malignant hemopathies.

Adolescent↗
PubMed1987

Prognosis in chronic lymphocytic leukemia: a retrospective multicentric study from the GIMEMA group.

Clinical and biological data were evaluated using Desu univariate analyses or Cox multivariate analyses in a series of 1,777 chronic lymphocytic leukemia (CLL) patients from an Italian Cooperative Group. In univariate analyses, age and sex of patients, presence of bone marrow (BM; greater than or equal to 50%), and peripheral blood (PB; greater than or equal to 60,000/microL) lymphocytosis, anemia (hemoglobin [Hb] less than 11 g/dL), thrombocytopenia (less than 100,000/microL), direct Coombs' test positivity, hepatomegaly, splenomegaly, and extent of lymph node involvement were shown to be of significant prognostic value. Multivariate analyses, through a stepwise procedure, showed that the most important prognostic variables are Hb, hepatomegaly, lymph node involvement, PB lymphocytosis, and age and sex of patients. Further covariates would produce an improvement having a nonsignificant P value. Based on the results of multivariate analyses, a four-step staging using the significant variables of the Cox model is proposed.

Adult↗
PubMed1986

Acute lymphoblastic leukemia hand-mirror cells with OKT 8 phenotype.

Acute lymphoblastic leukemia with hand-mirror cells (ALL-HMC) was diagnosed in a 20-year old patient. Cytochemical investigations revealed a positive reaction for PAS and acid phosphatase. Lymphoid blast cells were studied with various monoclonal antibodies in order to determine their derivation and differentiation. The data obtained (positivity for Leu 9, OKT 11 and OKT 8) suggest that blast cells were of T lineage with OKT 8 phenotype. This report supports the phenotypic heterogeneity of ALL-HMC.

Adult↗
PubMed1985

HTLV-I is endemic in southern Italy: detection of the first infectious cluster in a white population.

Human T-cell leukemia virus (HTLV-I) infection is observed among black and Japanese populations in well-delimited endemic spots in association with a high incidence of adult T-cell leukemia (ATL). We present evidence of HTLV-I infection in two ATL patients from southeastern Italy who have not travelled and who have no known relations abroad, and in 8% of non-leukemic controls from the same area. Thus, populations exhibiting HTLV-I infection appear more widespread than supposed up to now.

Antibodies, Viral↗
PubMed1985

T-helper phenotype chronic lymphocytic leukaemia and "adult T-cell leukaemia" in Italy. Endemic HTLV-I-related T-cell leukaemias in southern Europe.

Sixteen Italian patients with chronic T-cell lymphocytic leukaemia (T-CLL) and leukaemic T-helper phenotype lymphocytes (Thp-CLL) were investigated for serum antibodies against human T-cell leukaemia virus I (HTLV-I) or its integrated DNA sequences. Common features of this series of patients were an aggressive clinical course with poor response to treatment, high white blood-cell count, bone-marrow infiltration, splenomegaly, and chromosome abnormalities. Three patients had skin infiltration and one had hypercalcaemia. Immunological analysis showed a Thp (OKT4+) in all cases, and a heterogeneity, within the OKT4 population, of phenotypes and functional activities. Three patients had either HTLV-I integrated DNA sequences or anti-HTLV-I serum antibodies, or both. These patients had not received any blood transfusions, denied intimate contact with foreigners, and had always lived in small towns of central or southern Italy. Clinical and immunological findings in this series of patients suggest that both HTLV-I related and unrelated cases of Thp-CLL should be regarded as one disease arising from the same subpopulation of mature T-lymphocytes.

Adult↗
PubMed1985

Adenosine deaminase activity in peripheral lymphocytes of patients with gynaecologic malignancies.

Peripheral lymphocyte adenosine deaminase (ADA) activity was assessed in a group of 31 patients with gynaecologic malignancies (19 with carcinoma of the portio, 7 with endometrial adenocarcinoma, 3 with ovarian cancer, 1 with adenocarcinoma of the cervix, 1 with liposarcoma myxoide). 30 female subjects, aged 30 to 70 years, were studied as the control group. Lymphocyte ADA activity in the 31 patients ranged from 0 to 7 U/10(7) cells with a mean of 2.3 U/10(7) cells (normal values: range 2-5 U/10(7) cells; mean 2.8 U/10(7) cells). In cases where the enzyme was absent or far below the controls, a faster evolution of the disease was observed. We would point out that lymphocyte ADA activity in the patients under investigations shows a broad range of variability. Our preliminary observations would suggest that lymphocyte ADA assessment in a larger series of cancer patients may add further prognostic informations.

Adenosine Deaminase↗
PubMed1984

[Characteristics of the electrophoretic mobility of blasts from acute non-lymphoid leukemias].

The behaviour of electrophoretic mobility (E.M.) of the blast cells has been investigated in 15 cases of acute non lymphoid leukemias (A.N.L.L.), (eleven cases of M1 type and four M5 type). The fast and homogeneous E.M. of M5 type in comparison with M1 has been underlined. E.M. of M1 blasts has been reported in a wide range varying from a "normal" migration (similar to that of normal neutrophils and monocytes) to a "fast" one (similar to M5 type). It is suggested that M1 leukemic forms, morphologically recognizable as the same leukemic type, are different L.A.N.L. indeed.

Acute Disease↗
PubMed1983

[Prognostic usefulness of lysozyme in acute myelomonocytic leukemias].

Pre-treatment sera and urines from 17 patients with acute myelomonocytic leukemia (M-4 type) have been assayed for their lysozyme content. In addition periodical evaluations of the serum and urinary lysozyme levels have been performed during the clinical course of 10 patients. There was no correlation between initial lysozyme activity in the serum and response to chemotherapy, while periodical estimations of serum lysozyme activity, in the same patient, provide clinically significant prognostic information. Urinary lysozyme levels are closely related to serum lysozyme levels; therefore the urinary estimations of this enzyme activity provide no different prognostic evaluations than serum estimations.

Adult↗
PubMed1983

Acute lymphoblastic leukemia hand-mirror cells. Study of nine cases.

Acute lymphoblastic leukemia with hand-mirror cells (HMC) was diagnosed in nine adult patients. Blast HMC were seen only in the bone marrow (12-57% range). Cytochemical studies revealed a positive reaction to tartrate-sensitive acid phosphatase in the tail portion of the cells in seven cases, with a strong, localized cytoplasmic reaction in four. Leukemic cells lacked surface immunoglobulins and were E rosette negative in all cases. Normal levels of adenosine deaminase activity (ADA) were found in five of the seven patients. Electron microscope studies confirmed the hand-mirror shape of the cell. These HMC contained large numbers of mitochondria and microspikes in the handle portion of the cell. The patients failed to respond to initial conventional ALL chemotherapy, but the prolonged survival with passable health of the majority of these, despite their lack of complete remission, is emphasized.

Acid Phosphatase↗
PubMed1982

Platelet satellitism to basophils in a patient with chronic myelocytic leukaemia.

Platelet satellitism to basophil granulocytes was observed in a patient with chronic myelocytic leukaemia. This phenomenon occurred with peripheral basophil cells obtained from venous blood anticoagulated with EDTA. Previous reports have demonstrated platelet satellitism to polymorphonuclear neutrophils and monocytes but not to other types of white blood cells. The cause of the platelet satellitism in this CML case remains unclear. It is suggested that this rare phenomenon should be considered in evaluating the platelet number in CML.

Aged↗
PubMed1982

[Electrophoretic mobility in acute null cell lymphoblastic leukemia].

The electrophoretic mobility (E.M.) (evaluated by cytopherometer) of human normal lymphocytes, shows a group of cells with a fast mobility, identifiable as T lymphocytes, and a second group with a slow mobility which can be characterized as B lymphocytes. Six cases of Acute Lymphoblastic Leukaemia characterized by negativity of immunological markers and classified as "Null Cells", and ten cases of B cell Chronic Lymphocytic Leukaemia were studied in order to investigate the E.M. behaviour of leukaemic cells and so a comparison with T and B normal lymphocytes migration. The blasts of Acute Lymphoblastic Leukaemia show a E.M. nearly similar to the fast group of normal lymphocytes, while lymphocytes of the B cell Chronic Lymphocytic Leukaemia migrate as the slow component of normal lymphocytes. The possibility of the utilization of cell E.M. in addition to immunological and cytochemical data, into the characterization of Acute Leukaemias is suggested.

Adolescent↗