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Biomedical subjects

V Kliment

Publications and source records attributed to V Kliment.

At least 19 recordsLinked to original sources

Intestinal parasites: a study of human appendices.

Histological sections of 414 appendices were examined parasitologically. Enterobius vermicularis was found in 8.7%, eggs of Ascaris lumbricoides in 0.5%, trophozites of Dientamoeba fragilis in 4.8%, Endolimax nana in 2.2%, Entamoeba coli in 1% and cysts of Giardia intestinalis in 1.9% of cases. Appendicopathies associated with Enterobius were most frequent in the age group from 6 to 10 years (24.3%) and from 21 to 25 years (12.2%). Patients older than 15 years were practically women only. Dientamoeba was most frequent in the age group from 11 to 15 years (11.3%). In women D. fragilis was three times more frequent than in men. The coincidence of D. fragilis and E. vermicularis infections was 50%. No interactions were seen between the protozoans in the contents of the appendix and its mucous membrane. Statistical evaluation indicates possible etiologic role of E. vermicularis in the occurrence of acute appendicities. D. fragilis appears to be the most common intestinal protozoan parasite in Bohemia.

Adolescent

The possible mutagenic effect of the occupation of TV announcer.

In a group of TV announcers, a significant increase in the number of classical aberrations was detected in peripheral blood: 7.5 +/- 3.477% in comparison with 3 control groups (A - 7 healthy women matched for sex, age and place of residence, B - 9 members of TV technical staff, C - 9 actors and actresses) with a mean aberration level of 2.96 +/- 1.136. SCE numbers were not increased in the TV announcers compared to controls. An increased frequency of reproduction failure has also been noticed in TV announcers (females). A decrease in the level of aberrant cells was noticed when the announcer had been on holiday, although this was not statistically significant. The possible mutagens in the working environment of TV announcers are discussed; there is a suspicion that azodyes, present in cosmetics or other chemicals, may be the mutagenic factor.

Chromosome Aberrations

Computer-assisted analysis of human G-banded chromosomes.

The problem of data input into the computer was solved by means of an electromechanical scanner of our own design, which processes information from a positive photocopy. Classification of chromosomes in homologous pairs uses linear functions of discriminant variables. Evaluation of the quality of discriminant variables is based on multivariate generalization of analysis of variance and on Wilks' statistics. The success of the system described depends generally on the reproducibility of banding, on the loss of information during photographic process, on the parameters of the equipment used, as well as on the mathematical methods employed. The results were obtained from an analysis of 27 male and female diploid sets from the normal population and they are fully comparable with other systems, although no direct processing of the sets from the preparation is used.

Chromosome Aberrations