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Biomedical subjects

V K Jain

Publications and source records attributed to V K Jain.

At least 109 records · Page 6Linked to original sources
PubMed1989

Energy linked modifications of the radiation response in a human cerebral glioma cell line.

Effects of cellular energy metabolism on the radiation response of a cell derived from a human cerebral glioma have been studied under conditions of energy limitation produced by the presence of inhibitors of respiratory metabolism (KCN) and glycolysis (glucose analogues such as 2-DG, 5-TG, and 3-0-MG). Radiation 60Co induced DNA repair (Unscheduled DNA Synthesis) and micronuclei formation were studied as measures of radiation response. Glycolysis (lactate production) and levels of adenine and related nucleotides (UTP, GTP, ATP etc.) were measured as parameters of energy metabolism. Two 2-DG (5 mM) inhibited DNA repair and increased micronuclei frequency both in the presence and absence of respiration (KCN, 2 mM). Under similar experimental conditions, the presence of 2-DG also significantly reduced the cellular energy status. Five-TG and 3-0-MG on the other hand, neither significantly altered the energy status (sigma XTP) nor influenced the radiation response under respiratory proficient conditions. The results can be explained on the basis of a model postulating differential energy linked modulations of the repair and fixation processes acting on DNA lesions. Implications of the present results for the radiotherapy of brain tumors are discussed.

Adenosine Triphosphate↗
PubMed1989

2-Deoxy-D-glucose induced modification of chromosomal damage in UV-irradiated peripheral human leukocytes.

UV-irradiation (0.6 J/m2) of peripheral human leukocytes 27 hr after PHA-stimulation induced a considerable mitotic delay in the cultures. Approximately two thirds of the chromosomal aberrations induced by UV were gaps of the chromatid and isochromatid types. Treatment with glucose antimetabolite 2-deoxy-D-glucose (2-DG) alone did not induce any chromosomal damage. Presence of 2-DG (5 mM, equimolar with glucose) for 2 hr after UV-irradiation resulted in a significant reduction in the frequency of cells with aberrations. Decrease in the total aberrations per cell was also observed. The data are consistent with earlier observations that 2-DG reduces the manifestation of radiation damage in normal proliferating cells.

Adult↗
PubMed1989

Clofazimine induced nail changes.

Two cases of lepromatous leprosy with erythema nodosum leprosum who were on high doses of clofazimine, showed discoloration of nail plate, subungual hyperkeratosis and onycholysis. These nail changes gradually disappeared when the dose of clofazimine was reduced.

Clofazimine↗
PubMed1989

Surgery in spontaneous intra-cerebral haematoma.

Twenty-six patients of intracerebral haemorrhage (ICH) were treated by surgical evacuation of clot. Six patients were operated within 24 hour, four on the second day, seven between the third and fifth day and nine after five days. Their therapeutic out-come was compared with an equal number of age, location and severity matched group of patients of ICH who did not undergo surgery. Mortality in surgically treated group (16/26) was comparable to control group (20/26), however, follow up of survivors showed slightly reduced morbidity in operated group. Patients in grade III (level of consciousness) were the only ones who were benefited from surgery. The timing of the surgery did not affect the immediate mortality.

Adolescent↗
PubMed1989

Ventilatory functions in tropical pulmonary eosinophilia.

A study of ventilatory function in tropical pulmonary eosinophilia was carried out. Seventy five cases and 75 healthy controls were studied. Four types of ventilatory patterns were observed-restrictive (52%), mixed (33%), obstructive (4%) and normal (10.6%).

Female↗
PubMed1989

Xeroderma pigmentosum--report of four cases.

Four typical cases of Xeroderma Pigmentosum (XP) showing involvement of skin, eyes and oral mucous membrane are described. History of consanguinity was found in two cases. However, family history of XP was seen in only one case. The disease is often fatal before age of 10, but longevity in our patients is interesting.

Adult↗
PubMed1988

Electroretinograms in early fungal endophthalmitis.

To facilitate the early diagnosis of exogenous fungal endophthalmitis, we developed a rabbit model for Aspergillus fumigatus endophthalmitis. Six eyes of six New Zealand white rabbits were inoculated with forty spores of A. fumigatus. A control group of their six contralateral eyes received a similar but sterile inoculum. The rabbit eyes were evaluated with respect to clinical appearance and the electroretinogram. Clinically evident endophthalmitis developed in all six infected eyes at an average of five days after injection (range 3-7 days). Data samples each 48 hours showed transient b-wave amplitude elevations in three infected eyes. These were greater than two standard deviations above the mean pre-injection values (p less than 0.05). These amplitude increases preceded the onset of ophthalmoscopically recognizable infection and were observed at 2 to 5 days after injection (mean = 4 days). B-wave amplitudes in the infected eyes fell below two standard deviations of pre-injection values (p less than 0.05) at an average of 5 days (range 3-7 days) after injection, a corresponding to the onset of clinically obvious infection. At an average of 7 days after injection, all the infected eyes exhibited unmeasurable electroretinogram waveforms and severe infection. Histopathologic study of infected eyes showed extensive fungal infiltration of retina and vitreous tissues. The electroretinogram may be helpful in the early diagnosis of fungal endophthalmitis.

Animals↗
PubMed1988

Bilateral giant intracavernous aneurysms. Technique of unilateral operation.

We report a case of bilateral intracavernous giant aneurysms of which the right side was clipped by direct surgery. Our case was a 74-year-old woman who developed bilateral abducens palsy. Computed tomography and angiography showed bilateral intracavernous giant aneurysms. This patient was followed up in the outpatient clinic. She developed headache and right trigeminal neuralgia 2 years later. The trigeminal pain was quite severe and very disturbing to her. Repeat angiography showed an increase in the size of the aneurysms. An operation was carried out after a balloon Matus test monitoring electroencephalogram, somatosensory evoked potential, and clinical symptoms. The cavernous sinus was opened and the aneurysm was clipped. There was no postoperative complication except right ophthalmoplegia which resolved after 3 months. Headache and trigeminal neuralgia also disappeared.

Aged↗
PubMed1988

Leukemoid reaction in erythema nodosum leprosum in a leprosy patient.

A case of lepromatous leprosy with erythema nodosum leprosum (ENL) presenting as a myeloid leukemoid reaction is reported. Very high leucocyte count with immaturity of the cells in myeloid series was present in peripheral blood. High leucocyte alkaline phosphatase score, absence of hepatosplenomegaly and transient nature of leukemoid reaction differentiated it from chronic myeloid leukemia and acute myeloblastic leukemia. The possible mechanisms of leukemoid reaction in ENL are discussed.

Adult↗