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Biomedical subjects

V K Garg

Publications and source records attributed to V K Garg.

69 records · Page 4Linked to original sources

Effect of cations on intestinal nutrient transport in two teleosts.

The uptake of sugar and amino acids was affected by the presence of cations in the filling solution in both the fishes, Ophiocephalus and Heteropneustes. Under low Na+ concentration, the rate of transport decreased while an increase in Na+ concentration brought about its corresponding increase in both the fishes. Li+ was able to substitute Na+ to some extent in the filling solution in the transport of xylose, glycine and leucine. The replacement of Na+ by Li+ was more successful in xylose transport, in contrast to the transport of glycine and leucine. On the other hand, K+ was not able to substitute Na+ in the transport process. K+ inhibited the transport of glycine but did not that of xylose and leucine.

Animals↗

Distribution of glucose-6-phosphatase and 5-nucleotidase in the digestive system of two teleost fishes.

The distribution and histochemical localization of G6Pase and 5-nucleotidase in the different parts of the alimentary canal of two teleost fishes, Heteropneustes fossilis and Barbus sophore have been studied. The major sites of activity of the two enzymes are intestinal mucosa and liver. G6Pase is localized in the cytoplasm of the absorptive cells of the intestinal mucosa while 5-nucleotidase is present in the cytoplasm as well as nucleus and cell membrane. In the intestine, the anterior portion shows more stronger activity than in the middle and posterior portions. Mild activity is also noticed in the mucosa and gastric glands of the stomach. G6Pase activity is stronger than 5-nucleotidase activity in all the portions. Weak 5-nucleotidase activity is found in the submucosal connective tissue and the nuclei of the intestine. The goblet cells, muscularis abd serosa are negative in all the portions. G6Pase activity is stronger in the herbivorous fish Barbus sophore than in the omnivorous form Heteropneustes fossilis. In the liver, stronger activity of two enzymes is localized in the hepatocytes surrounding the sinusoids. Connective tissue and endothelial lining are negative.

Animals↗

Intestinal transport of fructose and glycine in Ophiocephalus punctatus.

The regional variations in the absorption of fructose and glycine have been studied in the intestine and pyloric caeca of the fish, Ophiocephalus punctatus in vivo, by preparing intestinal sacs. Diffusion of amino acids out of the intracellular compartment of the intestine was observed in all the portions of the intestine. The pyloric caeca showed maximum transport of fructose. Absorption of fructose was slightly more in the posterior intestine than in the anterior intestinal sac. Maximum transport of glycine took place in the anterior intestinal sac. Pyloric caeca adsorbed more glycine than did the posterior sac. For both nutrients, the rate of uptake did not differ significantly in the upper and the lower portion of the intestine. In all the portions, the transport of fructose was more rapid than that of glycine. The experiments revealed that though the nutrients are absorbed in the entire length of the intestine, the quantity absorbed varies from one portion to another and is dependent on the nature of the compound absorbed.

Animals↗

Histochemical study of lipid absorption in two teleost fishes.

Absorption of lipid in the different regions of the intestine of two teleost fishes has been studied histochemically at different time intervals of 4, 10 and 20 h. All the three portions of the intestine have the absorptive capacity. However, it varies from one region to another. The lipid has been absorbed in the form of lipoid particles and lipoid goblets. It is also recorded here that some lipoid droplets are absorbed by pinocytosis across the brush border membrane. The lipid absorbed was localized in the brush border cells, in intercellular spaces of connective tissue, in submucosa, in between serosa and muscularis, in the lumen of blood capillaries and mesentric lymphatic vessels but was absent in muscularis and serosa.

Animals↗

Airway obstruction in bronchiectasis and its reversibility--a study of 38 patients.

Thirty-eight patients (20 males and 18 females) with bronchiectasis were studied for the evidence of airway obstruction and its reversibility in response to subcutaneous terbutaline. There was an improvement in VC (8.56%), FEV1 (6.36%), PEFR (15%) and PEF 25-75% (18%) respectively. The difference being statistically significant in VC and highly significant in FEV1, PEFR and FEF25-75 as compared to initial values and in 6 patients the improvement in FEV1 was more than 15%. The improvement in airway obstruction was not related to the character of expectoration. It is suggested that all the patients of bronchiectasis should be assessed for the reversibility of airway obstruction and those showing a reversible pattern be treated accordingly.

Adult↗

Ultrasonic evaluation of pleural opacities.

Fifty patients of pleural effusion were subjected to radiological and ultrasonic examinations. The results of both examinations were compared and presented here. The study was undertaken to assess the comparative efficacy of ultrasonography in diagnosing pleural effusion.

Adolescent↗