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Biomedical subjects

V Insler

Publications and source records attributed to V Insler.

At least 127 records · Page 7Linked to original sources
PubMed1983

Human endometrium in cell culture: a new method for culturing human endometrium as separate epithelial and stromal components.

The present study describes a simple method for culturing human endometrium as separate epithelial and stromal components. Fifty-two samples of normal human endometrium have been initiated in tissue culture: endometrium from both the proliferative and luteal phases of the menstrual cycle showed satisfactory growth in vitro with a success rate of about 94%. Epithelial cultures remained viable for 60 days, while from stromal cells it was possible to establish cell lines. Both cell types possessed estrogen receptors. Epithelial cells showed no clear estrogen or progesterone response. Our observations suggest that this simple method for culturing human endometrium may serve as a tool in further investigations.

Connective Tissue↗
PubMed1983

Viral antibodies in maternal and cord sera.

Viral antibodies were determined in paired maternal and cord blood sera of 258 consecutive deliveries. Antibodies to Epstein-Barr virus (EBV), cytomegalovirus (CMV), herpes simplex type 1 (HSV-1), adenovirus (ADV), and varicella zoster (VZV) were tested by indirect immunofluorescence and to rubella (RUB) by hemagglutination inhibition. Analysis of the overall pattern of differences between maternal and cord blood showed a highly significant difference for all six viruses. Analysis regarding individual viruses showed significantly higher titers to CMV and RUB in cord blood, a higher titer to VZV in maternal blood, and similar levels of antibodies to EBV, HSV-1, and ADV in maternal and cord blood. Infants with one or more risk indicators (weight less than 3 kg, Apgar score less than or equal to 7, clinical jaundice, meconium-stained amniotic fluid, respiratory distress syndrome) were defined as "at risk." Infants free of such indicators were defined as "normal." Significantly lower antibody levels to all six viruses were found in both maternal and cord blood of the "at risk" as compared to the "normal" group, while the ratios between the maternal and cord blood levels remained similar. Birth defects were found to have no effect on antibody titers. These results indicate an efficient and selective transfer through the placenta of certain viral antibodies and the possible association of lower antibody production with the presence of risk indicators in the infants.

Antibodies, Viral↗
PubMed1983

The significance of single sporadic deceleration during a nonstress test.

The significance of single sporadic deceleration occurring during antepartum fetal heart rate monitoring was assessed. Single sporadic deceleration was defined as a drop of fetal heart rate of at least 40 bpm below baseline lasting for at least 2 min. During the year 1980, 7202 nonstress tests (NST) were performed in pregnant patients, with gestational ages ranging from 32-42 wk. Among these, 62 cases of single sporadic deceleration were observed. In 48 of these cases the single sporadic deceleration was followed by a normal reactive NST. In 14 patients NST following the single sporadic deceleration was non-reactive. In those cases oxytocin challenge test (OCT) was performed and was negative in 10 and positive in 4 cases. In the latter 4 patients labor was induced and all required cesarean section because of severe fetal distress. The remaining 58 women were followed up at the High-Risk Pregnancy Unit until spontaneous labor began, their deliveries were monitored throughout. In 8 cases out of the 58, fetal distress was observed during labor and in 4 of them cesarean section was required. The occurrence of single sporadic deceleration at gestational ages 32-42 wk is not an alarming sign providing the NST and/or O.C.T. are normal. However, the incidence of fetal distress during labor is significantly higher in fetuses with single sporadic deceleration as compared to the normal parturient population.

Female↗
PubMed1983

Clinical significance of sinusoidal fetal heart rate pattern.

Sinusoidal fetal heart rate pattern has been regarded as a sign of fetal jeopardy and 92 instances of this pattern were analysed. The morphologically different major and minor sinusoidal patterns were assessed with regard to their clinical significance as predictors of fetal compromise. There were 83 instances of minor sinusoidal pattern (amplitude of oscillation less than 25 beats/min) with only one antepartum fetal death. Major sinusoidal pattern (amplitude of oscillation greater than 25 beats/min) was diagnosed in nine patients and in six of them the fetus died before, during or after delivery. It is concluded that patients with minor sinusoidal heart rate patterns may be managed expectantly, whereas on the rare occasion when a major sinusoidal pattern is seen expeditious delivery is justified.

Apgar Score↗
PubMed1983

Transfer of viral antibodies from mother to infant.

Antibody titers to Epstein-Barr virus (EBV), cytomegalovirus (CMV), herpes simplex virus (HSV) Type 1, varicella-zoster virus (VZV) and rubella virus (RV) were determined in maternal and in newborn cord sera in 258 consecutive deliveries. Infants born with one or more of the following risk factors--weight less than 2.5 kg, Apgar score less than 7, clinical jaundice, meconium-stained amniotic fluid, birth defects and respiratory distress syndrome--were considered to be at risk. Similar geometric mean titers (GMT) of antibodies to EBV and HSV were seen in cord blood. Anti-VZV maternal antibody levels were significantly higher than those of cord blood (P less than 0.01). Levels of antibody to CMV and RV, the two viruses most implicated in fetal damage were significantly higher in cord blood than in maternal blood (P = 0.02, and P less than 0.001, respectively). Lower GMTs of antibodies to all five viruses were found in both maternal and cord sera of the group of infants at risk, as compared with infants without risk factors, while the ratio of maternal to cord blood antibody levels remained similar. It appears that inefficient production of antibodies correlates with the presence of risk factors in the newborn.

Antibodies, Viral↗
PubMed1982

The effect of estrogen and gestagen on the mucus production of human endocervical cells: a histochemical study.

The effect of hormonal stimulation on the intracellular mucus production of the endocervical cells was histochemically and morphometrically investigated. Twenty-seven cervixes from patients who had received estrogen or gestagen prior to hysterectomy were examined. "Upper" versus "lower" cervix and "small" versus "large" crypts were compared in both groups of patients. The histochemical analysis of mucins within the cells was based mainly on the combined alcian blue/periodic acid Schiff (AB/PAS) techniques at various pH levels. A semiquantitative estimation using cell counts and statistical evaluations was done for each staining method. The results indicate that sex hormones influence the chemical composition of endocervical mucus production. In addition, the nature of the intracellular mucus varies with the cryptal size and location within the cervical canal.

Alcian Blue↗
PubMed1982

Polyzoospermia: a definite pathologic entity.

Polyzoospermia has been repeatedly associated with reduced fertility and increased rates of spontaneous abortion. We examined semen samples of 1374 infertile men and found an incidence of 4.2% of semen with a sperm density exceeding 250 X 10(6) sperm cells/ml. The spontaneous pregnancy rate in 30 evaluated couples in whom the male partner presented with polyzoospermia was 38.7% and the spontaneous abortion rate was 25%. Sperm penetration tests revealed normal mucus-penetrating ability in 29 of 30 men examined. The reason for reduced reproductive performance in polyzoospermic men does not seem to be disturbed sperm mucus interaction.

Abortion, Spontaneous↗
PubMed1982

Is end-stage deceleration of the fetal heart ominous?

End-stage deceleration (ESD) defined as deep and sustained fetal bradycardia during the second stage of labour was observed in 55 patients. Fetal well-being and neonatal outcome were assessed with fetal-scalp pH, umbilical-vein pH and 1-min Apgar score values. Only six infants with pathological fetal heart-rate patterns before ESD had a 1-min Apgar score of less than 7 and an acidotic umbilical-vein pH. The remaining 49 babies were born in excellent condition, although when ESD persisted for more than 15 min umbilical-vein pH decreased.

Adult↗
PubMed1982

Outcome of pregnancy in 31 triplet gestations.

The course of gestation and labor and the outcome of 31 triplet pregnancies were analyzed. Early diagnosis is very important for improving the rate of fetal salvage in multiple pregnancy. The outcome of triplet pregnancies was better in the group of patients with induced ovulation than in the group with spontaneous ovulation. The most frequent antenatal complications were preterm labor (97%), pregnancy-induced hypertension (46%), and anemia (20%). In this series the favorable results were due to earlier diagnosis, prolonged hospitalization, and planned delivery, usually by cesarean section.

Adult↗
PubMed1981

Cesarean section--a review of two annual periods separated by a ten-year interval.

In the Soroka Medical Center in Beer-Sheba during the two annual periods 1969 and 1979, 693 cesarean sections were performed, of which 633 are presented. The main reasons for the rise in cesarean section rate from 2.6% in 1969 to 8.0% in 1979 were evaluated. Cesarean sections were performed more often for fetal indications during 1979 than in 1969, reflecting the change in our approach to the management of the fetus at risk. The increase in the cesarean section rate was concomitant with a drop in the perinatal mortality from 23/1000 in 1969 to 13.1/1000 in 1979.

Breech Presentation↗
PubMed1981

Fetal heart rate patterns and scalp blood pH as predictors of fetal distress.

Repeated samplings of scalp blood pH were obtained from 62 fetuses in whom continuous fetal heart rate monitoring was indicative of fetal distress. In all, 182 scalp blood samples were evaluated. Umbilical cord blood pH was determined immediately after delivery in 48 cases. In addition to heart rate abnormalities, fetal metabolic changes were considered in determining the mode of delivery. Vaginal delivery was allowed in 74.2% of the patient in our series and only 25.8% had cesarean sections. Repeated scalp blood pH sampling proved to be an important adjunct to continuous fetal heart rate monitoring in increasing the accuracy of diagnosis of fetal distress.

Apgar Score↗
PubMed1980

Clinical evaluation of fetal movements.

Fetal movement was examined in 200 pregnancies to establish normal patterns, influencing factors, possible clinical value and practical application. The mean number of movements per hour was 26 in hospitalized and 40 in ambulatory patients. These values remained fairly steady throughout pregnancy except for a significant decrease in the week preceding labor. Individual variations were large. The number of movements was uninfluenced by parity and fetal sex but was lower for small-for-gestational-age babies, in mothers suffering from both hypertension and diabetes and in mothers treated with diazepam. Fetal movement count may be used as a parameter of fetal well-being.

Body Weight↗
PubMed1980

Sperm storage in the human cervix: a quantitative study.

Twenty-five women scheduled for hysterectomy for nonmalignant disease participated in the study. Sperm storage in endocervical crypts was examined in three groups of patients: nine women pretreated with estrogen and inseminated with normal semen, nine women pretreated with gestagen and inseminated with normal semen, and seven women pretreated with estrogen and inseminated with abnormal semen. The number of crypts containing spermatozoa (colonized crypts) and the sperm density per crypt were examined in serially sectioned cervices. In estrogen-pretreated cervices both the percentage of colonized crypts and the sperm density were significantly higher than in gestagen-pretreated cervices. Large and giant crypts proved to be the main storage facility for spermatozoa. The localization of crypts along the endocervical canal did not influence sperm storage. The quality of semen appeared to be of critical importance to sperm storage. The percentage of colonized crypts and sperm density were severly reduced in patients inseminated with abnormal semen.

Cervix Mucus↗
PubMed1980

Diagnostic and prognostic value of retinal hemorrhages in the neonate.

Ocular fundi of 410 newborns were examined for retinal hemorrhages within 24 hours after delivery. The purpose of this study was to investigate whether the course of pregnancy and management of delivery influence the incidence of neonatal retinal hemorrhage (RH) and whether this incidence could be reduced by electronic monitoring of delivery. To assess the prognostic value of neonatal RH for future development of the child, the physical, motor, and psychologic states of 252 infants were examined 6 to 18 months following delivery. The incidence of RH was 37.3% and was not influenced by maternal age, parity, gestational age, course of pregnancy, birth weight, or electronic monitoring of delivery. The mode of delivery did influence the incidence of RH. Gesell developmental schedule showed no correlation between ocular findings and later development of the child.

Delivery, Obstetric↗
PubMed1980

Management of patients with a live fetus and cessation of fetal movements.

Sixty-seven patients with a history of no fetal movements for at least 12 hours and a live fetus were managed according to a fixed protocol. Twelve of these patients were found to have meconium-stained amniotic fluid and/or abnormal unstressed or oxytocin-stressed cardiotocograms; all of them were delivered within 24 hours of a live infant. The remaining 55 patients had no meconium and normal unstressed and stressed cardiotocograms, and their pregnancy, was allowed to continue. All felt fetal movements again and were delivered of live babies one to ten weeks later.

Amniotic Fluid↗