Biomedical subjects
Publications and source records attributed to V I Sedov.
The study of enterococcal species present in 16 species of synanthropic flies revealed the predominance of Str. faecalis and its variants, a high percentage of fly populations being infected. The spectrum of enterococci was proved to depend on the living conditions of the flies. The percentage of Musca domestica with the infected digestive tract was found to vary with changes in the age composition of the fly population.
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The most stable differential signs of enterococci are: growth in the medium at pH 10.2, growth in broth containing 40% bile, citrate utilization, resistance to 0,05% potassium tellurite, 2, 3, 5-triphenyltetrazolium chloride (TTC) reduction, the staining of colonies (plaques) on a medium with manganese, iron and zinc salts, glycerine fermentation under anaerobic conditions, mannite fermentation, the presence of hemolysin, of the proteolytic enzyme, and mobility. Combined differential-diagnostic nutrient medium permits to determine simultaneously 5 enterococci signs--resistance to nalidixic acid and to crystal violet, TTC reduction, hemolytic and proteolytic activity. The suggested scheme of enterococci laboratory diagnosis including a set of hard nutrient media poured into multisection Petri dish is simple reliable and accessible for any bacteriological laboratory.
The author worked out a method of enterocinotyping on enterococci. A possibility of enterococcus typing by means of a set of enterocinogenic enterococcus strains was shown. A mobile enterococcus 4 (10 p) strain, possessing a high antagonistic activity, is suggested for enterococcus identification. Differentiation of enterococcus species by the enterocine sensitivity proved to be impossible.
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The role of enterococci in the development of chronic obstructive bronchitis was determined. Enterococcus faecalis, subspecies zymogens and liquefaciens, were found to be the etiological factors of chronic obstructive bronchitis both as the primary cause of the disease and, probably, the cause of secondary hospital infection. Enterococci isolated from patients had some signs of pathogenicity, their characteristic feature being multiple drug resistance. Chronic obstructive bronchitis caused by these microorganisms was accompanied by decreased immune responsiveness and the development of autoimmune processes.