Biomedical subjects

V Borderie

Publications and source records attributed to V Borderie.

63 records · Page 4Linked to original sources

PubMed1997

[Surgical treatment of astigmatism caused by penetrating keratoplasty using the Hanna arcuate keratome].

PURPOSE: High postkeratoplasty astigmatism is a common postoperative complication which can limit the final functional result. Arcuate incisions are a possible surgical treatment. They can be performed with the arcuate keratome which provides regular incisions. The aim of this study was to evaluate the results obtained with this device in the correction of high postkeratoplasty astigmatism. METHODS: We retrospectively studied ten eyes operated for high postkeratoplasty astigmatism with the Hanna arcuate keratome. Arcuate keratomy procedures were performed on the graft button in all. Before surgery, mean uncorrected visual acuity was 0.07 +/- 0.05. Best spectacle-corrected visual acuity was 0.33 +/- 0.20 and mean subjective cylinder was 6.1 +/- 1.71 D. RESULTS: After one month postoperatively, the mean best spectacle-corrected visual acuity (0.45 +/- 0.20) was significantly improved (p < 5%) and mean subjective cylinder (2.85 +/- 1.29 D) was significantly decreased (p < 5%). Vector analysis showed a 5.59 D (+/- 3.63) mean astigmatism correction. Modifications of the spherical equivalent were not statistically significant. Astigmatism irregularity was not modified. CONCLUSION: Arcuate keratotomy carried out with the Hanna arcuate keratome is effective in reducing high postkeratoplasty astigmatism. It is easier to perform than the standard manual technique. However, astigmatism correction predictibility should be improved.

Adult↗

PubMed1997

[Cultures of corneal epithelium on hydrogel for synthetic epikeratoplasty].

PURPOSE: To improve the LLH480 hydrogel cytocompatibility and to assess the interactions between corneal epithelium and biomaterials. METHODS: Rabbit corneal epithelium organotypic cultures were carried out on hydrogel samples coated with fibronectin, laminin, type IV collagen, or heparan sulfate proteoglycan. The control group consisted of epithelial cultures carried out on hydrogel with no coating. Cellular migration was quantified, and statistically analyzed. 8-day cultures were processed for transmission electron microscopy. RESULTS: Epithelial migration on hydrogel was significantly increased (p < 0.001) in the heparan sulfate proteoglycan group, significantly decreased (p < 0.05 and p < 0.001) with laminin or type IV collagen coating, and showed no significant modifications in the fibronectin group. Transmission electronic microscopy showed a monolayer of epithelial cells adherant to the surface of the hydrogel. Deposit of extracellular matrix and sketch of adhesion focal points were also noted. CONCLUSION: LLH480 cytocompatibility can be improved by heparan sulfate proteoglycan coating.

Animals↗

PubMed1997

[Astigmatism after penetrating keratoplasty. Videokeratoscopic analysis on a series of 60 grafts].

PURPOSE: To study the factors which induce post keratoplasty astigmatism. To assess the reliability of different methods in the astigmatism measurement and to study the visual acuity predicting factors. METHODS: We retrospectively studied the corneal topography in 60 eyes with penetrating keratoplasty after suture removal, using the CAS* (Eye Sys). The diagnosis was keratoconus in 65% of the cases and bullous keratopathy in 15% of the cases. The graft was secured with a single running suture in 38.3% of the cases, interrupted sutures in 23.3% of the cases, or a combination of both running and interrupted sutures in remaining 28.3%. RESULTS: The suture method, diagnosis and surgeon did not influence subjective refraction nor visual acuity. The topographic pattern correlated with subjective cylinder (rs = 0.52 p = 0.02). The refractive power cylinder ("Holladay diagnostic summary") correlated well with subjective cylinder (rs = 0.81 p < 0.001) and visual acuity (rs = -0.63; p < 0.001). The Javal keratometry remains the best method to measure astigmatism axis (rs = 0.58; p < 0.001). CONCLUSIONS: The Holladay diagnostic summary (refractive power) is a useful tool for evaluating qualitative outcome of corneal transplantation.

Adolescent↗

PubMed1995

[Penetrating keratoplasty after graft preservation in organ culture at +37 degrees centigrade. 1-year results].

PURPOSE: To evaluate the clinical results of corneal transplantations using organ-cultured donor corneas. MATERIAL AND METHODS: We retrospectively studied our 50 first penetrating keratoplasties performed with corneas organ-cultured for 2 to 5 weeks at +31 degrees C (Inosol medium, Opsia). The average follow-up is 11.6 months. The main indications for grafting were bullous keratopathy (56%) and keratoconus (26%). RESULTS: At 1 year, the graft survival rate was 81%. The causes of graft failure were immune rejection (n = 3), non immune secondary endothelial decompensation (n = 3) and uncontrolled glaucoma (n = 1). The average endothelial density during the second year was 1,349 cell/mm2. It was 1,852 in the group of keratoconus and 751 in the group of bullous keratopathy. The average corrected visual acuity was 20/66 at 12 months. It was 20/45 in the group of keratoconus and 20/125 in the group of bullous keratopathy. The average astigmatism was 4.9 D, but running suture was still present at 12 months in most patients. Compared with graft survival after storage at +4 degrees C (retrospective paired control group), graft survival was higher after organ culture, but the difference does not reach the statistical significant threshold (p = 0.07). CONCLUSIONS: Organ culture is able to ensure the quality of corneal endothelium and then avoids non immune primary graft failure.

Aged↗

PubMed1994

[Microbiological safety and endothelial quality control during preservation of corneal grafts at +31 degree C].

Over a 10-month-period, 59 corneas were organ-cultured at + 31 degrees C and 7 were preserved at + 4 degrees C. Among the 59 organ-cultured corneas, 41% (24) were grafted and 25% (15) developed an infection during preservation. At the time of surgery, preservation medium and scleral rim were tested for microbiology. Infection during preservation was bacterial (80%, 12/15) and fungal (20%, 3/12). Scleral rims were sterile in 96% (24/25) of the grafted corneas preserved at + 31 degrees C but 34% (2/6) at + 4 degrees C (p < 0.01). Preservation media were sterile in 100% (24/24) of organ-cultures at + 31 degrees C but in 83% cases (5/6) of preservations at + 4 degrees C (NS). The percentage of endothelial dead cells increased with postmortem enucleation delay (r = 0.43; p = 0.03). The average endothelial cell loss during organ-culture was 11.2% (+/- 10.4). It correlated with endothelial density (r = 0.42; p = 0.04) and with percentage of endothelial dead cells (r = 0.43; p = 0.04) after enucleation. Organ-cultured at + 31 degrees C was able to assess graft sterility in almost 100% of the cases but corneas are lost due to infection. Graft sterility is not usually possible with preservation at + 4 degrees C.

Aged↗

PubMed1993

[Astigmatism and its treatments].

Astigmatism is a refractive error related to corneal asphericity. Congenital astigmatism is most frequent, and its correction with spectacles has been known for a long time. Contact lenses may sometimes be useful, but they may not allow a satisfactory visual correction. Anterior segment surgery (cataract, penetrating keratopasty, etc.) may often cause postoperative astigmatism. Improvement in ophthalmic surgery, and the patient's need for a rapid and effective visual recovery (as anatomical result is obtained), have highlighted prevention and treatment of post-surgical astigmatism. Astigmatism treatment is complex and varies according to its intensity and cause. Refractive surgery, modifying corneal shape, may be an elegant solution in cases where vision is low, in spite of a perfect corneal transparency.

Astigmatism↗

PubMed1993

[Ultrastructural study of human cornea preserved in organ culture media at +31 degrees C].

Organ culture becomes a standard method of corneal graft preservation. The aim of the study was to evaluate the preservation injuries induced by organ culture. We examined 12 organ cultured human corneas. Corneas were preserved in the medium for 2 to 21 days. Corneas showed some abnormalities: numerous light vacuoles, mitochondrial swelling and increased cell thickness in all cells of the cornea, sloughing of the external and medium epithelial cell layers. We observed normal endoplasmic reticulum, Golgi apparatus and nucleus. These preservation injuries are moderate and reversible.

Aged↗

PubMedDate not supplied

[Use of topical cyclosporin in microcrystalline keratopathy due to Streptococcus].

Infectious crystalline keratopathy is an exceptional but serious complication of penetrating keratoplasty. Streptococcus viridans and a steroid treatment are very often found. The treatment is always lengthy and difficult. The high number of bacteria and the steroid treatment, necessary to prevent rejection, worsen the infection. We report a case of Streptococcus crystalline keratopathy that evolved, despite the treatment, simultaneously toward abscess and acute rejection. Topical cyclosporin treatment was used to maintain an immunosuppression without worsening the infection.

Administration, Topical↗

PubMedDate not supplied

Nonfreeze myopic keratomileusis for myopia in 158 eyes.

BACKGROUND: A prospective evaluation of non-freeze myopic keratomileusis is reported. METHODS: One hundred and fifty-eight eyes of 98 consecutive patients underwent nonfreeze myopic keratomileusis, with BKS 1000 (Eyetech-M.V.A.A.G, Balzers, Liechtenstein) refractive set. The preoperative myopia ranged from -6.25 to -28.00 D. Mean follow up was 591.3 days (range, 90 to 1500 days). RESULTS: The logarithmic mean preoperative spectacle-corrected visual acuity was 0.48 +/- 0.31 (20/40), and 0.44 (20/50) +/- 0.30 after 2 years and longer, whereas mean uncorrected visual acuity was 0.32 +/- 0.28 (20/70) in 34 of 82 (41.5%) eyes. After 2 years and longer, 21 of 82 (25.6%) eyes were within 1.00 D of emmetropia, and 43 of 82 (52.4%) were within 2.00 D. The subjective spherical equivalent refraction confidence interval at 90% was 9.28 D (-6.85 to +2.43 D). No refractive instability was detected during follow-up. We detected a trend toward improvement of spectacle-corrected visual acuity with time. However, after 2 years and longer, there was an increase in astigmatism of more than 1.00 D, when compared to the preoperative values, and 14 of 82 (17%) eyes lost two or more lines of spectacle-corrected visual acuity (statistically significant: p < .01). CONCLUSION: The nonfreeze myopic keratomileusis procedure, with BKS 1000, substantially reduces moderate to high myopia, but predictability of refractive outcome is only fair, and the frequency of optical complications including irregular astigmatism is higher than desired.

Adolescent↗