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V Borderie

Publications and source records attributed to V Borderie.

At least 55 records · Page 3Linked to original sources
PubMed2000

[Acute closure-angle glaucoma after treatment with ipratropium bromide and salbutamol aerosols].

We report a case of acute angle-closure glaucoma in a patient treated with bronchodilator nebulization. An 82-year-old man with chronic obstructive bronchopathy was treated for acute respiratory decompensation with salbutamol and ipratropium bromide aerosols. Twenty-four hours after beginning the treatment, the patient developed acute angle-closure glaucoma which resolved rapidly with appropriate treatment. The case emphasizes the importance of precautionary measures (waterproof glasses and inhalation masks). In addition patients with a high risk of angle-closure glaucoma should be detected prior to prescribing bronchodilating aerosols.

Acute Disease↗
PubMed2000

[Marginal corneal perforation associated with herpes simplex virus in a patient with essential cutaneous porphyria].

We report a case of herpetic perforated peripheral corneal ulcer in a patient with cutaneous porphyria and hepatitis C. The patient underwent peripheral lamellar keratoplasty. Antiherpetic antibodies were found at a significantly higher level in the aqueous humor than in the serum. Outcome was favorable with antiviral treatment. We discuss the causal role of the hepatitis C virus, porphyria and herpes simplex.

Anti-Bacterial Agents↗
PubMed2000

[Mooren ulcer].

Explore the source record for details and available documents.

Adrenal Cortex Hormones↗
PubMed2000

Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone.

PURPOSE: To investigate whether human corneal epithelial cells express the glucocorticoid receptor (GR) and to assess the influence of dexamethasone (DEX) on these cells. METHODS: Human corneal epithelial cells were cultured in medium supplemented with various concentrations of DEX (ranging from 10(-10) to 10(-4) M). Cell proliferation was analyzed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfop henyl) -2H-tetrazolium inner salt (MTS) assay at 2, 4, and 6 days of culture. Apoptosis was studied by nucleus labeling using a fluorescent dye and immunostaining by APO 2.7 at 6 days of culture. GR mRNA was detected in corneal epithelium and cultured corneal epithelial cells by means of reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemical staining of the epithelial cells was performed with a monoclonal anti-human GR. RESULTS: RT-PCR and immunocytochemistry showed the expression of GR (mRNA and protein) in corneal epithelial cells. DEX significantly increased corneal epithelial cell proliferation with concentrations ranging from 10(-10) to 10(-6) M, with a maximum effect at 10(-7) M (P < 0.005). However, DEX also induced apoptosis of cultured corneal epithelial cells at any concentration used. CONCLUSIONS: These results indicate that human corneal epithelial cells express the GR and proliferate in response to DEX stimulation which also induces corneal epithelial cell apoptosis.

Apoptosis↗
PubMed1999

In vitro effects of dexamethasone on human corneal keratocytes.

PURPOSE: To investigate whether cultured human keratocytes express the glucocorticoid receptor (GR) and to assess the influence of dexamethasone (DEX) on these cells. METHODS: Human keratocytes were cultured in medium supplemented with various concentrations of DEX (ranging from 10(-10) to 10(-4) M). Cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-s ulfophenyl)-2H-tetrazolium inner salt (MTS) assay at 2, 4, and 6 days of culture. Some experiments were performed in the presence of mifepristone (RU38486), an antiglucocorticoid molecule. The early phase of apoptosis was studied by means of keratocyte staining with a fluorescein conjugate of annexin V and propidium iodide, and cells were analyzed by flow cytometry. Glucocorticoid receptor mRNA was detected in keratocytes by means of reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemical staining of the cells was performed with a monoclonal anti-human GR. RESULTS: RT-PCR and immunocytochemistry showed the expression of GR (mRNA and protein) in cultured keratocytes. Dexamethasone significantly increased keratocyte proliferation with concentrations ranging from 10(-9) to 10(-5) M, with a maximum effect at 10(-7) M (P < 0.005). Dexamethasone's proproliferative effect was inhibited by RU38486. However, DEX also induced apoptosis of cultured keratocytes at any concentration used. CONCLUSIONS: These results indicate that cultured human keratocytes express the GR and proliferate in response to DEX stimulation (10(-9)-10(-5) M), which also induces keratocyte apoptosis.

Apoptosis↗
PubMed1999

[Treatment of unilateral limbal stem cell deficiency syndrome by limbal autograft].

PURPOSE: To evaluate the improvement of the ocular surface after limbal autograft in patients with unilateral limbal stem cell deficiency related to chemical burns. MATERIALS AND METHODS: Limbal autograft was performed in five patients with unilateral limbal stem cell deficiency related to chemical burns. Thereafter, four patients underwent penetrating keratoplasty. The limbal graft was obtained from the fellow eye, and was secured with interrupted sutures. Patient follow-up ranged from 10 to 47 months. Limbus and corneas were studied by means of light microscopy. RESULTS: All five patients reported subjective improvement. Vascularization decreased in one cornea. Visual acuity improved in one eye and did not change in the remaining four eyes. After penetrating keratoplasty, graft reepithelialization was achieved after respectively 3, 4, 21, and 30 days. Light microscopy showed the presence of goblet cells in the limbal epithelium in four cases. After limbal autograft, the corneal epithelium was devoid of goblet cells in three out of four cases. CONCLUSION: Limbal autograft improves the ocular surface and the prognosis of subsequent penetrating keratoplasty in patients with unilateral limbal stem cell deficiency related to chemical burn.

Adult↗
PubMed1999

[Effect of sodium hyaluronate administered at the end of penetrating keratoplasty on reepithelialization of the graft].

PURPOSE: To investigate the effect of sodium hyaluronate on reepithelialization time after penetrating keratoplaty. METHODS: We prospectly studied 56 consecutive penetrating keratoplasties. One group of patients (n = 22) received dexamethasone ointment at the end of surgery. The second group (n = 34) received sodium hyaluronate and topical dexamethasone. Postoperative reepithelialization time and graft central thickness were recorded. RESULTS: There was no statistical difference between both groups for epithelialization (4.6 + 3.2 days in the hyaluronate group and 4.4 + 2.3 days in the dexamathasone group) and for graft thickness at day one (710 + 85 microns for the former group and 713 + 84 microns for the latter group) and at day of reepithelialization (663 + 80 microns for the former group and 703 + 99 microns for the latter group). The epithelium aspect was different at the first postoperative day between both groups. CONCLUSION: The use of sodium hyaluronate at the end of penetrating keratoplasty improves the graft epithelium aspect at the first postoperative day, but it does not influence reepithelialization time. Further studies are needed to investigate the mechanism of sodium hyaluronate effect on corneal epithelium.

Aged↗
PubMed1999

[Comparison of EyeSys videokeratoscope algorithms in the evaluation of idiopathic and postoperative astigmatism].

PURPOSE: To compare the accuracy and reproducibility of the Eye Sys videokeratoscope algorithms for analyzing idiopathic and surgery-induced astigmatism analysis. METHODS: Refractive astigmatism, videokeratoscopy (axial, tangential and refractive power), autorefractometry, autokeratometry, and keratometry were recorded in 20 patients with idiopathic astigmatism, 40 patients who had undergone cataract surgery and 40 patients who had undergone penetrating keratoplasty. For each eye, 2 successive videokeratoscopy were recorded. RESULTS: Both cylinder and axis provided by the tangential algorithm are significantly less reproducible than the cylinder and axis provided by the axial and refractive algorithms (P < 0.001). Cylinders provided by the axial and refractive algorithms showed a stronger correlation with subjective cylinder (rs > 0.89; p < 0.001) than the cylinder provided by the tangential algorithm (rs = 0.66; p < 0.001). Both keratometric axis and autokeratometric axis showed the strongest correlation with subjective axis (rs > 0.92; p < 0.001). The accuracy and reproducibility were higher for the topographic "bow tie" patterns than for the other topographic patterns. CONCLUSION: The axial and refractive algorithms of the Eye Sys videokeratoscope are more accurate and reproducible than the tangential algorithm for analyzing idiopathic or surgery-induced astigmatism.

Algorithms↗
PubMed1998

Light microscopic evaluation of human donor corneal stroma during organ culture.

PURPOSE: To try to facilitate evaluation of corneal stroma during organ culture by means of light microscopy. METHODS: Corneal stroma of 53 consecutive organ-cultured corneas was studied by means of light microscopy during endothelial quality control. Out of 9 corneas with bad stromal evaluation, 2 were studied by means of transmission electron microscopy, and 7 were grafted. From the remaining 44 corneas with a normal light microscopic appearance, 35 were grafted. RESULTS: Stromal abnormalities consisted of bright visible structures with a cell-like shape corresponding to keratocyte injuries (i.e. cellular edema, light and dark vacuoles, cell membrane disruption and, finally, internal cytolysis) as observed by TEM. At 3 months postoperatively no clinical differences between the two groups of transplants were observed. CONCLUSION: Corneal stroma can be evaluated qualitatively and easily by means of light microscopy during organ culture. Further studies are needed to investigate whether the presence of lysed keratocytes in the graft's stroma actually influences the outcome of transplantation.

Adult↗
PubMed1998

[Culture of human keratocytes. Influence of culture conditions and ultrastructural aspects].

BACKGROUND: To investigate the influence of fetal calf serum (FCS) and fibroblast growth factor (FGF) on human keratocyte growth in vitro and cell differentiation, and to describe cultured human keratocyte ultra-structure. METHODS: Human keratocytes were cultured in TC 199/Ham F12 media, supplemented or not with 10% FCS, aFGF, and bFGF. Keratocyte growth was studied. Cultured keratocytes were analyzed by means of immunochemistry and transmission electron microscopy. RESULTS: Without fetal calf serum, cell population doubling occurred after 7 days of culture and no alpha smooth muscle-actin cell expression was observed. With serum, cell population increased by 1 log after 7 days of culture and all of the cells were alpha SM-actin + bFGF or aFGF-addition to the serum-containing medium resulted in a dramatic decrease in this alpha SM-actin expression. Nuclei were found to be oval and regular in cross-sections, and round and indented in frontal sections. Numerous cytoplasmic organelles were observed, as were cell expansions, gap junctions, omega-shaped structures, and fenestrations. Cultured keratocytes synthesized collagen fibers and filaments. CONCLUSION: Fetal calf serum allows human keratocytes to grow with a myofibroblast cell phenotype, whereas addition of FGF results in a fibroblast cell phenotype. Ultrastructure of cultured keratocyte is similar to that observed in situ.

Actin Cytoskeleton↗
PubMed1998

[Ultrastructural and immunohistochemical study of 3-dimensional cultures of human keratinocytes on a collagen gel].

PURPOSE: To investigate the ultrastructural and immunochemical features of three-dimensional cultures of human keratocytes in collagen gel matrix. METHODS: Human keratocytes were obtained from primary cultures of stromal explains. They were cultured in bovine type I collagen gel matrix for 6 weeks. Keratocyte-populated gels were analyzed by means of immunochemistry and transmission electron microscopy. RESULTS: Human keratocytes cultured in collagen gel matrix developed processes and formed networks of connecting cells. They showed positive staining for vimentin, collagen I, V, and VI, and connexin. Electron microscopy showed elongated cells with processes and gap junctions. Keratocytes synthesized collagen fibrils and filaments. No fibrils' organization similar to that observed in the normal human corneal stroma (i.e. parallel bundles of collagen fibrils) was observed. CONCLUSION: Ultrastructure and immunochemical phenotype of three-dimensional cultures of human keratocytes in collagen gel matrix are similar to those observed in situ. These cultures represent a useful in vitro model to study the different corneal stroma components.

Animals↗
PubMed1997

In-vitro development of corneal epithelial cells on a new hydrogel for epikeratoplasty.

This study demonstrates the adhesion and growth of bovine corneal epithelial cells on the surface of a new hydrogel. The hydrogel, containing 78% of water and 22% of AN-69 polymer (poly(acrylonitrile-sodium methallyle sulfonate)), was obtained by phase inversion of polymer-dimethylformamide solution in physiological saline (0.9% NaCl). Experiments were also carried out using hydrogel treated with arginine, human albumin and collagen IV. Covering of hydrogel samples by epithelial cells was completed within ten days, with good cell viability. The epithelial cells spread out and formed a consistent cell layer, confirmed by immunocytochemistry experiments against cytokeratins. Transmission electron micrographs showed numerous desmosomes between cells and the presence of some membrane differentiations at the cell/hydrogel interface. This study suggests therefore that the hydrogel might be suitable for the development of artificial epikeratoplasty grafts.

Journal Article↗
PubMed1997

[Value of implantation in the capsular bag during combined operation of penetrating keratoplasty and cataract surgery].

PURPOSE: To evaluate the clinical results of the triple procedure performed using phacoemulsification. METHODS: We prospectively studied 23 consecutive triple procedures carried out during a 3-year period. The average follow-up was 18 months. The main indications for grafting were Fuchs' endothelial dystrophy (30%) and infectious keratitis (26%). RESULTS: A capsulorrhexis was performed in 70% of the cases whereas a "can opener" was performed in the remaining 30%. A divide and conquer phacoemulsification method was used in 43% of the cases. Hydrodissection was used for lens removing in 27% of the cases. The posterior lens capsule was inadvertently broken and a flexible anterior chamber lens was inserted in one case. Twenty-two posterior chamber lenses were inserted in the capsular bag (16) or in the sulcus (6). The estimated 1-year graft survival was 76.8% overall, and 92.9% when high-risk recipients were excluded. Causes of graft failure were immune rejection (n = 4), persistent epithelial defect (n = 1) and uncontrolled glaucoma (n = 1). The average endothelial density during the second year was 1,401 cell/mm2. Average corrected visual acuity was 20/45 at 12 months. Average astigmatism was 3.9 D. No retinal complication was observed. CONCLUSIONS: In-the-bag placement of posterior chamber lens is a safe and useful technique during the triple procedure. Phacoemulsification may be useful when the nucleus is hard and voluminous.

Aged↗
PubMed1997

[Dural carotid-cavernous fistula with uveal effusion syndrome].

We report the case of a patient with a dural carotid-cavernous fistula. The examination showed a proptosis, a chemosis, an increased intraocular pressure and a choroidal detachment. The diagnosis was confirmed by arteriography. The treatment consisted of the embolisation of the feeders originating from the external carotid artery. The ocular improvement was only partial, but choroidal detachment regressed.

Arteriovenous Fistula↗