Biomedical subjects
Publications and source records attributed to V A Evseev.
Nonspecific immunomodulators (complete Freund's adjuvant and bovine serum albumin) produced an antistress effect on mice during immobilization stress. Immunization with a serotonin-protein conjugate produced no antistress effect.
In this review the authors analyze neuroimmune aspects of the pathogenesis of such neuropsychic diseases as drug addiction, alcoholism, epilepsy, and pathologic pain. The role of pro-inflammatory cytokines in the development of pathological pain and epilepsy is discussed. The authors analyze their own data on the role of antibodies to neuromediators, such as catecholamines, serotonin, and glutamate, in the development of various forms of neuropathology, and outline the prospects of immunotherapy of the mentioned forms of neuroimmunopathology, including immunotherapy with conjugate on homological protein carrier.
Procedures for anterior spine fusion and posterior instrumental spinal fixation have been developed for the surgical treatment of childhood tuberculous spondylitis complicated by severe kyphotic deformity. The optimum method of the instrumental fixation is the use of multisupport constructions with the heterodirectional action of corrective efforts (in 15 children). Anterior osteoplastic stabilization of the vertebral column, by applying brace cortical grafts with saddle-shaped hollows on the tips of its ventral projection, was applied in 6 children. A significant and steady-state correction of kyphosis was achieved in all the cases; there were no complications of anterior spine fusion and posterior instrumental fixation.
We demonstrated a possibility of inducing antibody production against serotonin in high titers in rats by active immunization with serotonin conjugated with a homologous protein carrier (rat serum albumin) and with a foreign protein carrier (BSA).
We studied the effect of single intraperitoneal treatment with antibodies to glutamate on pentylenetetrazole-induced acute generalized epileptiform activity in C57Bl/6 mice. The antiepileptic effect was observed 1.5 and 24 h after administration of antibodies to glutamate in doses of 10, 25, and 50 mg/kg. This treatment increased the thresholds of clonic seizures and tonic phase of seizures with lethal outcome.
We studied the effect of immunization with a serotonin-bovine serum albumin conjugate on parameters of stress reaction to immobilization stress in rats. Active immunization was accompanied by changes in parameters reflecting animal resistance to emotional stress. The observed changes can be interpreted as a decrease in individual resistance to emotional stress. Active immunization of rats with a serotonin-bovine serum albumin conjugate was accompanied by production of autoantibodies against serotonin and dopamine. The role of autoantibodies against dopamine in modulation of the effect of immunization with serotonin-bovine serum albumin conjugate on the stress reaction in rats is discussed.
The neuroimmune aspects of epilepsy pathology are analyzed in the survey. The pathogenetic role of proinflammatory cytokines and of antibodies to neuroagents as well as of antibodies to the NMDA-receptor and glutamate was defined within the development of epilepsy. It is for the first time that the data of the authors' independent research are presented, which testify to the anti-epilepsy activity of antibodies to the glutamate.
The possibility of long-term changes in the innate characteristics of the behavior of mice of different genotypes (C57Bl/6 and BALB/c) after active immunization with dopamine-BSA conjugates was studied. These experiments revealed significant differences in the effects of anti-dopamine antibody on behavioral responses in an open field and neurotransmitter contents of brain structures in mice of different lines. Immunization with the dopamine-protein conjugate led to increases in the functional activity of the corresponding brain receptors, to different extents in mice of different lines.
We studied the effect of active immunization with glutamate-bovine serum albumin conjugate on acute generalized epileptiform activity evoked by single administration of corazol. Antibodies to glutamate produced an antiepileptic effect and elevated the threshold for clonic seizures and tonic phase of lethal seizures. In BALB/c mice this effect was more pronounced than in C57BL/6 mice.
The scientific review is devoted to an analysis of neuro-immune aspects of the pathological pain and to the role of disregulation between the central nervous system (CNS) and the immune system in triggering the mechanisms of such pain. The importance of anti-inflammatory cytokines (interleukins, and tumor necrosis factor) as well as of autoantibodies to neuro-mediators in the pathogenesis of different forms of hyperalgetic conditions is evaluated. New data are discussed, which are related with the possibility of modulating the antibodies to neuro-transmitters (serotonin and catecholamines) of experimental neuropathic pain syndromes.
A new mode has been proposed to correct deformities in tuberculous spondylitis complicated by more than 50 degrees kyphotic deformity. The mode involves extended repair of the vertebral column, supplemented by the use of Luque-modified posterior multisupport hooked contractors. The mode ensures correction deformity to 47 degrees. The procedures of a surgical intervention are described.
Immunization of BALB/c mice with glutamate-BSA conjugate reduced anxiety and improved passive avoidance retention, while in C57Bl/6 mice immunization disturbed passive avoidance retention, but had no effect on anxiety. Interstrain differences in the shuttle box behavior were found between control animals.
We studied the effects of active immunization with glutamate-protein conjugates on behavioral reactions of C57Bl/6 and BALB/c mice in the open-field test and on animal anxiety in the tail suspension test. Antiglutamate antibodies produced different behavioral changes in mice of these strains.
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Effects of active immunization with the serotonin-protein conjugate on open-field behavior, learning and retention of the conditioned passive avoidance response, neurotransmitter contents in brain structures, and functional activity of central serotonin receptors were studied on C57Bl/6 and BALB/c mice. Serotonin antibodies produced opposite effects on the studied parameters in genetically different mouse strains.
The effect of antiserotonin antibodies on basal electrocorticogram was studied in electrophysiological experiments on rats. Intracortical injection of 10 microg antiserotonin antibodies into the sensorimotor cortex induced epileptiform activity in this area. It is assumed that antiserotonin antibodies modulate activity of cortical neurons due to both binding serotonin molecules and interaction with serotonin receptors.
Dysregulation of neuroimmune connections is a primary or secondary pathogenic factor of some CNS diseases. Autoimmune aggression is typical of multiple sclerosis, Alzheimer's disease, and epilepsy, while dysregulation characterized by enhanced production of autoantibodies to neurotransmitters and activation of cell factors is characteristic of alcoholism and drug abuse. In experimental models of alcoholism and drug addiction, protective effects of antiserotonin antibodies are mediated by immune cells stimulated by these antibodies. These effects can be used in the therapy of various forms of neuroimmunopathology by the method of adoptive immunotherapy.
Experiments on C57Bl/6 mice kept under conditions of free choice between alcohol and water for 4 and 12 months showed that adoptive transfer of splenocytes extracorporeally stimulated with arginine vasopressin suppressed alcohol intake.