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Biomedical subjects

U Wagner

Publications and source records attributed to U Wagner.

At least 217 records · Page 12Linked to original sources
PubMed1986

An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.

In the human breast cancer cell line MCF-7, we observe estrogen induction of the stable transfected Xenopus vitellogenin A2 gene. An estrogen-responsive element (ERE) could be defined by using a vitellogenin-chloramphenicol acetyltransferase hybrid gene in transient transfection experiments. The ERE is located in the 5' flanking region and is able to confer estrogen inducibility to the thymidine kinase gene promoter. By 5' and 3' deletions we have determined a 35 bp sequence sufficient for high stimulation by estradiol. Even 18 bp give a small estrogen response. The 35 bp ERE contains the palindromic sequence 5'GGTCACAGTGACC-3' as an essential element. The fact that the ERE of a frog gene functions in human cells demonstrates that signals and factors involved in the control have been conserved during evolution.

Acetyltransferases↗
PubMed1986

[Significance of the urethral pressure profile in the diagnosis of urethral diverticulum].

Diverticulum of the urethra was diagnosed by us in ten women during the past two years. In nine of these, the urethral pressure profile showed a characteristic course: In the region of the diverticulum--confirmed subsequently by radiography--a drop in pressure was recorded as a sign of damage to the wall. The value and importance of the individual steps in the diagnosis of diverticula are discussed. Suspicion of a diverticulum in the urethra can be raised by careful anamnesis and by means of the urethral pressure profile. This suspicion can be safely confirmed or refuted via urethrography using the double-balloon catheter.

Adult↗
PubMed1986

[Histochemical phenoloxidase demonstration in tumors of the melanocytic system].

Investigations of tissues from a naevus pigmentosus and 5 malignant melanomas were carried out. In malignant melanomas, the number of phenoloxidase reactive cells and the quantity of preformed pigment are very variable. It is discussed whether the in vitro phenoloxidase reactive cells and the in vivo pigment formation can be interpreted as expression of a cell mediated immunological defence reaction.

Adolescent↗
PubMed1986

Physicochemical hair conformation of patients with Sanfilippo disease type IIIA.

Sanfilippo disease type IIIA is an inborn error of metabolism with a deficiency in the heparan sulfamidase. Besides severe psychomotor retardation hair changes are obligatory. Hair is found to be coarse like a brush. We applied X-ray diffraction and infrared spectroscopy to characterize the conformation of hair samples of Sanfilippo patients. In healthy subjects as well as in the affected hair samples we found the wave numbers of structural relevance 1450, 1500, 1630, 1730, the pair 2337 and 2362, the quadruplet 2850, 2870, 2917, 2930 and 3080 cm-1. Also on X-ray diffraction analysis no differences could be detected. Though morphological-macroscopically and microscopically-changes were described for Sanfilippo hair samples, we could not find any change in supramolecular structure. The physical properties of coarseness of those hair specimen seems to be due to differences in the structural assembly of hair fibres and storage of heparan sulfate.

Child↗
PubMed1986

[Gastrointestinal complications in cystic fibrosis: meconium ileus equivalent].

In contrast to bloatedness and steatorrhoea the meconium ileus equivalent is a less well known gastrointestinal complication in cystic fibrosis and thus less frequently correctly diagnosed. The term, first used by Jensen in 1962, notifies partial or complete obstruction due to increasing viscosity of gut contents. The occurrence of the meconium ileus equivalent increases with age (approx. 10%), recurrences are possible. Among 73 patients with cystic fibrosis 8 patients with ileus equivalents were observed, two of them had a recurrence Surgery was performed only in one case. In all other patients the intestinal obstruction was relieved by oral and enema administration of N-acetylcysteine.

Acetylcysteine↗
PubMed1986

[Failure to thrive as a main symptom of intracranial tumors in early childhood].

In the course of only three years we studied three children, who presented for a long time with failure to thrive as the only symptom of an intracranial tumour. Up to 24 months elapsed from the first manifestation at the age of four, ten and eleven months to the final diagnosis. A suprasellar tumour was discovered once, an infratentorial one twice. In spite of lower caloric intake a more rapid weight gain was documented after neurosurgery. The intracranial tumour is an important part of differential diagnosis in infants and young children not thriving satisfactorily.

Astrocytoma↗
PubMed1986

Quantitative EEG: investigation in children with end stage renal disease before and after haemodialysis.

Changes in brain function of 9 children (6 males and 3 females) ages 7 to 14 years (mean 12 years) with end stage renal disease (ESRD) were investigated before and after haemodialysis treatment, utilizing computer assisted spectral analysis of the scalp-recorded EEG. A control group of age-matched healthy children was studied as well. Statistical analyses demonstrated that ESRD children exhibited more Delta and Theta activity, less Beta activity, a slower dominant frequency of the Alpha activity as well as a slower centroid of the total activity before treatment than the controls. These findings suggest a deterioration of vigilance as characterized by Head. Haemodialysis decreased slow activity and increased Alpha and Beta activity, thereby inducing an improvement of brain function.

Adolescent↗
PubMed1985

Reaction of human leukocytes after in vitro contact with a glucocorticoid.

The used hormone prednisolone bisuccinate causes in the concentration range of 2-1,000 micrograms/ml visible changes of the cell morphology and enzymatic activity in all leukocytes. These alterations appear immediately after the application of the glucocorticoid and remain nearly constant during the time of investigation (1 h). The changes in the cell morphology and the loss of enzymatic activity increase with enhanced hormone concentration. The following reproducible morphological changes caused by the hormone comprise swelling, vacuolization and deformation of the nuclei with concomitant release of nuclei material. In addition, cloverleaf and fircone-like structures originate in connection with the formation of small bubbles.

Cell Aggregation↗
PubMed1985

Changes in urethral closure pressure after radical hysterectomy for cervical cancer.

The behaviour of the urethral closure pressure at rest (UCP) both before and 14 days and 3 months after radical surgery for cervical cancer was examined. The results were compared with those of other authors. In contrast to the latter we were only able to establish a constant pressure drop in patients using a transurethral catheter at the time of the first postoperative measurement. Patients using a suprapubic catheter showed non-uniform postoperative UCP-changes. This pattern was comparable to that found after simple abdominal hysterectomy, performed for other reasons, in which a transurethral catheter had been in place for a maximum of 60 h. In contrast to our predecessors we were unable to confirm a causal relationship between surgical damage to the sympathetic system and postoperative falls in pressure, except after extremely radical surgery. The cause appears to be a complex process involving wound healing problems (edema, infection, scar formation and organ position changes) and direct traumatization of the urethra (transurethral catheter).

Female↗
PubMed1985

[Initial experiences with transvaginal sonography of the urethra and bladder within the scope of the diagnosis of incontinence].

Female urinary incontinence can be visualized only insufficiently be means of static methods such as the lateral cystourethrogram. Detailed information on physiology and morphology or pathological abnormalities can be gained only by means of dynamic methods, where transvaginal or transrectal sonography could become the method of choice. The essential advantages of this method over the conventional imaging methods are discussed.

Female↗
PubMed1985

[Retardation problems within the scope of prematurity].

Among 804 short-weight infants (birth weight less than 2500 g) born at the Department of Obstetrics and Gynaecology, Medical School, Friedrich-Schiller University, Jena, from 1. 1. 1978 to 31. 12. 1981, one-third were small for gestational age babies. These hypotrophic infants showed, in relation to premature infants (appropriate for gestational age) a higher prenatal and intranatal mortality (statistically not significant). A frequent severe foetal distress mainly on the background of chronic or subacute placental insufficiency implied a higher rate of obstetrical operations by the hypotrophic infants. This was particularly evident by the incidence of caesarean sections (24.7% : 15.2% respectively). The premature hypotrophic infants had the highest caesarean section incidence, amounting to 30.3%. Postnatal adaptation proved more favourable with the small for gestational age babies than with the premature group. There was in fact a statistically significantly lower rate of asphyxiated infants detectable by means of APGAR-score less than or equal to 7 five minutes after labour and a lower neonatal and late mortality in spite of "acidotic morbidity", which was statistically significantly higher in the group of small for date infants (p less than 0.05). We consider as possible cause a higher average duration of gestation with approximately average birth weights. The difference in the clinical behaviour between the premature-hypotrophic and hypotrophic infants were smaller than between the premature-eutrophic and hypotrophic infants. This contradictory behaviour before, during and after labour requires the specialist to be capable of meeting the diagnostic and therapeutical requirements in every respect.

Acid-Base Equilibrium↗
PubMed1985

[Gonadal and extragonadal yolk sac tumor].

Yolk-sac tumours are germ-cell tumours with gonadal and extragonadal location. Histologically they consist of primitive cellular forms configurated as a loose network. These tumours are of extreme malignancy and should be treated as radical as possible. Surgery should be combined with anticancer chemotherapy. However, with adequate treatment only children suffering from testicular tumours and not older than 2 years of life have a fair chance to survive. 3 cases are dealt with in detail.

Castration↗