Biomedical subjects

Tomasz Krecicki

Publications and source records attributed to Tomasz Krecicki.

26 records · Page 2Linked to original sources

PubMed2004

[Individual sensitivity to the mutagenic agents in patients with larynx cancer].

Patients with different cancers, induced mostly by numerous mutagenic factors, present increased genetic susceptibility to mutagenic agents known as "hidden chromosomal instability". This type of genetic instability can be detected in vitro after treating chromosomes with clastogenic substances, for example by using "bleomycine test". The purpouse of our study was to evaluate a hidden chromosome instability in patients with squamous cell carcinoma of larynx (SCCL) and to establish correlations between genetic results and both histological grade of the tumor and clinical progression of disease. Cytogenetic analysis of 126 of patients diagnosed with SCCL and 98 of controls was performed in vitro on peripheral blood lymphocytes. The analysis of sensitivity to bleomycine of larynx cancer patients in relation to histological grade of the tumor proved, that increased numbers of chromosome breaks as well as and percentage of aberrant metaphases are correlated with the tumor grading. Mean number of chromosome breaks per cell (b/c) in patients with larynx cancer was 1,01 (+/- 0.43), while in the control group was 0.74 (+/- 0.29) per cell (chi2 p < 0.001). Percentage of aberrant metaphases (% am) in patients with SCCL was 42.18 (+/- 11.61) and 36.05 (+/- 10.22) in the control (chi2 p < 0.001). The group of hypersensitive patients (b/c > 1) consisted of 51.5% percent of larynx cancer patients, while in the control, hypersensitivity occurred only in 20.4% of subjects (chi2 p < 0.001). Possible chromosome instability (0.8 < b/c < 1) was found 11.9% patients with SCCL and in 20.4% of controls (not statistically significant). Increased sensitivity to bleomycine in patients with SCCL in comparison to the control group was found. Most sensitive to bleomycine was the group of patients diagnosed with SCCL graded G2 (Scheffe's test p < 0.05)

Antimetabolites, Antineoplastic↗

PubMed2004

[Two cases of unknown aetiology oedema-hypertrophy changes in the larynx].

We have described two cases of oedema and hypertrophic changes of the larynx which cause remains unknown. We have shown in our paper diagnostic difficulties connected with these cases. A brief review of the literature is presented. The oedema and hypertrophic changes might be connected with inhalations of marijuana smoke.

Adult↗

PubMed2003

Expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in laryngeal squamous cell carcinomas.

Matrix metalloproteinases (MMPs) that have a proteolytic activity against the components of extracellular matrix (ECM) play an important role in the invasive and metastatic spread of tumors. The role of MMPs and tissue inhibitors of MMPs (TIMPs) in laryngeal squamous cell carcinoma (LSCC) has not been elucidated sufficiently. The aim of the present study was to evaluate the correlation between the expression of collagenase-1 (MMP-1), collagenase-3 (MMP-13) and TIMP-1, as well as the clinicopathological features of LSCCs. The expression of collagenases and TIMP-1 was examined immunohistochemically in 50 cases of surgically obtained specimens of primary LSCCs. Analyses indicated that LSCC cells as well as stromal cells expressed MMP-1, MMP-13 and TIMP-1 immunostaining. Overexpression of TIMP-1 occurred more frequently in non-metastasizing cases (P=0.009). TIMP-1 and MMP-1 staining correlated significantly with the histologic type of LSCC. The keratinizing type of carcinomas exhibited higher TIMP-1 protein expression than the nonkeratinizing variety (P=0.01). TIMP-1 staining was associated with the grade of differentiation, since it was found predominantly in well and moderately differentiated carcinomas (P=0.04). The findings confirm that expression of analyzed MMPs and TIMP-1 is characteristic of LSCC and that these enzymes contribute to the progression of tumors. TIMP-1 upregulation might exhibit lower metastatic potential in LSCCs and is linked rather with an early stage of tumor progression. It seems also that TIMP-1 expression is dependent on the grade of differentiation.

Adult↗

PubMed2003

Investigation of normal and malignant laryngeal tissue by autofluorescence imaging technique.

OBJECTIVE: During oncogenesis significant changes in tissue properties occur that result in alteration of fluorescence characteristics of the tissue. The differences in autofluorescence between normal and malignant tissues offer new possibilities in detecting epithelial pathological lesions. AIM: The aim of our study was to determine the usefulness of autofluorescence technique in diagnostic of laryngeal tumors. MATERIAL AND METHODS: Fluorescence in larynxes from 10 laryngectomized cancer patients was induced by excitation light at 360-450 nm wavelength. As a light source, mercury lamp and laser (404 nm) were used. The CCD camera captured the fluorescent light emitted. Normal epithelium and cancer tissue were also analyzed on fluorescence microscopy. RESULTS: Laryngeal carcinoma tissues when excited with ultraviolet or blue light generate attenuated or absent autofluorescence in the green light band compare with normal epithelium where strong signal around 505 nm was detected. Average value of maximal autofluorescence intensities was 26.2V (range 18-35) and 4.4V (range 2-8) in healthy and cancerously changed mucosa respectively. Spectral analysis revealed that autofluorescence signal from unchanged mucosa (around 505 nm) was significantly higher compared to cancer tissue (P=0.005). CONCLUSIONS: It seems that autofluorescence imaging may be a supplementary tool in diagnosis of laryngeal tumors.

Adult↗

PubMed2003

Histological and electron microscopic investigation of Reinke's edema.

Reinke's edema is a benign lesion of vocal fold affecting subepithelial space. This paper describes the histological features of Reinke's edema on the basis of an extensive number of cases (203 women and 58 men). In 10 cases the electron microscopic examination was performed. Edema of subepithelial tissue was present in 138(62%) cases. This phenomenon was observed more frequently in women than in men (p=0.01). In the subepithelial tissue there were a numerous wide vessels with oedematous endothelium. Leukoplakia and dysplasia of epithelium were present in 21(8%) and 16(6%) specimens, respectively. Leukoplakia was detected more often in men than in women. This relation was close to statistical significance (p=0.055). The presence of dysplastic lesions of the epithelium was correlated with the age of the patients and smoking habit (p=0.0042, p=0.0021). Electron microscopic investigations revealed loosened intercellular junctions and widening of intercellular spaces, especially in basal and spinous layers.

Adult↗

PubMed2003

[Laryngeal symptoms in patients with gastroesophageal reflux diseases].

Gastroesophageal reflux disease (GERD) is considered an important etiological factor of numerous larynx dysfunctions. The goal of our study was to describe larynx status of patients with GERD before and after treatment. Forty-five patients with GERD symptoms entered our study. GERD was confirmed in 24-hours pH-metric examination. Patients were treated with proton pump inhibitors in doses chosen accordingly to GERD severity. All patients underwent endoscopic evaluation of the larynx and acoustic voice analysis before and after the treatment. GERD was diagnosed in 35 out of 45 patients. Acoustic voice analysis revealed that voice disorders appeared more often in patients with severe than moderate or mild refluxes (p = 0.023). Laryngeal changes found in endoscopic evaluation also depended on reflux severity (p = 0.05). The voice quality improved (p = 0.031) and the changes in larynx diminished after anti-reflux treatment (p = 0.016).

Female↗

PubMed2002

Quantitative evaluation of angiogenesis in laryngeal cancer by digital image measurement of the vessel density.

OBJECTIVE: the aim of our study was determination of the relationship between angiogenesis and clinical as well as histological features in laryngeal cancer. METHODS: we used two different methods of estimation of the amount of microvessels in the series of 55 cases, e.g. classical count of endothelial cells group (h-MVD) and digital image measurement of the vessel density (VD). RESULTS: neither h-MVD nor VD correlated significantly with clinical features of the tumour. The results of VD examination correlated significantly with the existence of nodal metastases (P=0.02). The relationship between h-MVD and N status was on the statistical borderline (P=0.07). Multivariate analysis of Cox's proportional hazards model revealed that only N status correlated significantly with patients' survival. CONCLUSION: our results indicate that measurements of angiogenesis in laryngeal cancer may be of some value in predicting N status in laryngeal cancer patients. This issue should be confirmed in prospective studies.

Aged↗

PubMed2002

[Bilateral chemodectoma--a case report].

Chemodectoma is exceptionally rarely tumor comes from the parasympathetic part of nervous system. Cases description first of all concern multiple, single tumors localized on the neck, occasionally somewhere else. Tumors expansion in internal carotid vein aperture or in ear provoke serious complications from cranial nerves paralysis to cerebellum lesion. In this paper authors present a case of bilateral chemodectoma. Surgical treatment has required--not only simple tumor excision but also internal carotid artery reimplantation.

Adult↗