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Tomasz Krecicki

Publications and source records attributed to Tomasz Krecicki.

At least 19 recordsLinked to original sources
PubMed2006

Corticosteroid-induced laryngeal disorders in asthma.

BACKGROUND: Inhaled corticosteroids have proven to be the most effective agent available in treating bronchial asthma, and such treatment is believed to be very safe. Concerns regarding side effects of inhaled corticosteroids usually focus on potential systemic effects, where local side effects are often overlooked. The purpose of this study was to analyze and assess the influence of inhaled corticosteroids on the vocal cords of patients treated for bronchial asthma. MATERIAL/METHODS: Fifty patients (mean age: 50 years, range: 22 to 83 years) suffering from asthma and receiving corticosteroidal inhaled agents entered in this study. All of the patients underwent detailed videoscopic examination of the larynx. None complained of any laryngeal disorders or dysfunction before the diagnosis of asthma. All of the patients were non-smokers. RESULTS: Significant changes in the laryngeal status were observed. Changes included atrophy of laryngeal mucosa, vocal fold atrophy, and vocal fold bowing. CONCLUSIONS: Damage to the larynx is an important factor in patients with asthma treated with inhaled corticosteroids, which elicit apoptosis of the epithelium.

Adrenal Cortex Hormones↗
PubMed2006

[Expression of adhesive molecules, angiogenesis markers and protein products of suppressor genes in neoplasms of oral cavity and maxillary sinus].

INTRODUCTION: Adhesion molecules, angiogenesis markers and protein products of suppressor genes are potential prognostic indicators in different type of tumours. The purpose of this study was to analyze relations between expression of these markers and clinical as well as histological features of oral cavity and maxillary sinus tumours. MATERIAL AND METHODS: Measurements of expression of CD44, TP53, Nm23 oncoproteins and angiogenesis markers were performed. Forty-three patients treated in years 1985-2000 in the Department of Maxillofacial Surgery of the University Hospital in Wroclaw entered our study. Thirty-two of them were treated for oral cavity cancers. Remaining eleven patients were treated for maxillary sinus cancers. RESULTS: We have shown, there was a positive correlation between the number of the vessels and presence of the nodal metastases; between histological grading and VCAM expression; between CD44 expression and total surface area of the blood vessels. There was also correlation between total surface area of the blood vessels and patients' survival time.

Biomarkers, Tumor↗
PubMed2005

Brain auditory evoked potentials in children with Down syndrome.

UNLABELLED: Researchers have been examining the condition of the auditory organ in people with Down syndrome for many years. The aim of this work was an objective evaluation of the hearing threshold and a functional evaluation of the hearing pathway in children with Down syndrome without any disturbances in the middle ear. MATERIAL: Seventy children with Down syndrome, aged from 2 months to 17 years. Brain auditory evoked potentials (BAEP) were performed. The peak latencies in children who had a normal BAEP pattern at 70 dB sHL were analysed. Peaks I-III and interpeak latencies I-III were significantly shortened in Down syndrome children up to the age of 1 year, as compared to older children with Down syndrome and to the control group. Peak III and interpeak latencies I-III were significantly longer in older children with Down syndrome in comparison to the control group. CONCLUSIONS: Hearing loss in children with Down syndrome is more frequent than in healthy children. The average latency values of BAEP established for healthy children should not be used as a reference for children with Down syndrome. The shortening of the latency values in BAEP results of children with Down syndrome under 1 year of age may be connected with accelerated maturation of the nervous system or anatomical and/or functional disturbances of the central nervous system. BAEP is a valuable method that allows for the objective evaluation of the hearing threshold and functional condition of the hearing pathway in subjects who do not cooperate during testing.

Adolescent↗
PubMed2005

Hormonal markers of aging in men with laryngeal carcinoma.

BACKGROUND: Cancer of the larynx, a frequent neoplasm in older people, occurs several times more often in men than in women. Surprisingly, the highest incidence of the disease is observed in the period in which concentrations of a number of hormones (eg, androgens, growth hormone) decrease. Our objective was to look for differences in hormonal markers of aging between men with laryngeal carcinoma and healthy control subjects. METHODS: Seventy-eight men with cancer of the larynx and 51 healthy age-matched controls were recruited for the study. In each of the examined men, serum concentrations of total testosterone, free testosterone, dihydrotestosterone, dehydroepiandrosterone sulfate, steroid-hormone binding globulin, estradiol, and insulin-like growth factor type 1 (IGF-1) were determined. RESULTS: Men with laryngeal carcinoma had lower serum concentrations of IGF-1 (136 +/- 75 vs 318 +/- 141 ng/mL, p < .000001), lower free testosterone (11.95 +/- 5.38 vs 15.48 +/- 4.96 pg/mL, p < .001), and lower dihydrotestosterone/total testosterone ratio (0.07 +/- 0.06 vs 0.09 +/- 0.04, p < .05) than healthy controls had. CONCLUSIONS: Somatopause seems to be more evident in men with laryngeal carcinoma than in age-matched controls. In our observation, low concentration of IGF-1 predicted the presence of laryngeal carcinoma more than low concentration of free testosterone did.

Adult↗
PubMed2005

[The evolution of epidemiology and clinical characteristics of laryngeal and hypopharyngeal carcinoma in Poland from 1991 to 2001].

On the base of retrospective analysis of 12,888 cases of carcinoma of larynx and hypopharynx, diagnosed in 19 ENT Departments in Poland from 1991 to 2001, the assessment of basic epidemiological data, including the localization of tumor and stage of local and clinical advancement of the disease at the time of diagnosis has been conducted. In analyzed period of 11 years the trends to change of the mentioned above parameters has been examined. The significant increase of female patients in this period was observed, with average proportion M:F = 8:1. The glottis localization of carcinoma dominated (47.6%), followed by supraglottis (40.8%) and pyriform fossa (7.8%), with significant increase of pyriform fossa tumors in the analyzed period of 11 years. In the majority of cases the carcinoma of larynx and hypopharynx was diagnosed in the advanced stage (T3 + T4) of local disease, with the highest percentage in localization within the pyriform fossa (81.0%), and the lowest percentage in glottis tumors (45.6%). The regional lymph nodes metastases has been diagnosed in 46.7% of the analyzed group, with the highest percentage in tumors localized in pyriform fossa (82.9%), and the lowest percentage in tumors of glottis localization (33.1%). In the 11 years time the significant drop down of N0 cases and tendency to increase of N2 and N3 in the supraglottis localization of tumor. The distant metastases in the analyzed group at the time of diagnosis has been registered in 2.0%, with the highest percentage in posterior pharyngeal wall (7.6%) and pyriform fossa (7.4%). The authors postulate the renewal of prospective study on epidemiology, clinical characteristics and treatment results of larynx and hypopharynx carcinoma in Poland.

Adenocarcinoma↗
PubMed2005

[Autofluorescence laryngoscopy in the diagnosis of laryngeal cancer--early results].

BACKGROUND: During oncogenesis significant changes in tissue properties occur that result in alteration of fluorescence characteristics of the tissue. The aim of our study was to determine the usefulness of autofluorescence technique in diagnostic of laryngeal epithelium lesions. MATERIAL AND METHODS: 40 patients admitted to the Department of Otolaryngology Wroclaw Medical University due to different laryngeal lesions were included into the study. They underwent white light laryngoscopy followed by autofluorescence videoendoscopy. AF was induced by blue light of xenon short arc lamp and processed by a D-light AF System (Storz). Autofluorescence and white light images were immediately assessed and later compared with to histopathological findings. RESULTS: Normal laryngeal mucosa showed a typical green fluorescence signal. Squamous cell carcinoma displayed a diminished green fluorescence and in some cases presented reddish-yellow color. CONCLUSIONS: We revealed that autofluorescence laryngoscopy is very helpful, supplementary tool in diagnosis of laryngeal pathology. Autofluorescence laryngoscopy is noninvasive technique designed especially for diagnostic of early and premalignant lesions.

Carcinoma, Squamous Cell↗
PubMed2005

[Analysis of vocal fold paresis in the patient material of the Department of Otolaryngology in Wrocław from 1993 to 2002].

The aim of this paper was analysis of vocal fold paresis in the patients referred to ENT Department in Wrocław in the years 1993-2002. The study was performed on the base of 304 patients--209 women and 131 men. The average patient's age was 56 years. In all patients phoniatric examination and videostroboscopy were carried out. There were 38% cases of iatrogenic paresis mainly caused by thyroid surgery and cardiosurgery. In 30% causes we found the diseases which caused paresis (mainly infections, cancers, lesions of the central nervous system and injury of head, neck and chest). In about 30% causes we have not found the cause of paresis. Both vocal fold paresis in 98% was caused by thyroid surgery. Left vocal fold paresis was more often then paresis of the right side of vocal fold.

Adolescent↗
PubMed2005

[Changes of laryngeal mucosa in the course of pemphigus vulgaris].

Blister diseases are chronic autoimmune reactions connected with formation of intraepithelial blisters. Pemphigus vulgaris (PV) change is appear most often, almost 80% of all cases. Erosions on mucosa appear as first symptoms at 50-70% patients. Blisters occurring on the skin are typical for this illness and usually come into with weeks or months with delay in relation to the changes on mucous membranes. In this work we have described character and location of changes on mucous membranes at 5 patients with PV, diagnosed based on clinical symptoms and confirmed in immunofluorescent investigations.

Aged↗
PubMed2005

[Nasopharyngeal carcinoma coexistent with lymph node tuberculosis, diagnostic difficulties--case report].

Nasopharyngeal carcinoma occurs about 0.25-0.5% all malignancies in Poland. From the mids of a group more frequent are undifferentiated cancer (about 70%). The authors describe a case of undifferentiated nasopharyngeal cancer in 17-years old woman, which is connected with lymphonodular tuberculosis. They point to precise diagnostics of lymphonodular metastases, looking for primary carcinoma focus and difficulties in diagnosis.

Adolescent↗
PubMed2004

Inactivation of the cyclin-dependent kinase inhibitor 2A (CDKN2A) gene in squamous cell carcinoma of the larynx.

Defects in the system controlling the cell cycle can lead an increased proliferation of cancer cells. The aim of our study was to analyze the relationship between genetic changes leading to inactivation of the CDKN2A gene and subsequent alteration of protein expression in squamous cell cancer of the larynx (SCCL) in connection with the clinical and histopathological course of the disease. Analysis was carried out on DNA isolated from the blood and primary larynx cancer cells of 62 patients. To investigate loss of heterozygosity (LOH), PCR fragment analysis was applied. The size and quantity of fluorescent PCR products were evaluated in an automated sequencer. Specific chemical methylation with sodium bisulfite in a sequential PCR reaction (MSP) was applied to analyze promoter methylation. Cancer tissue sections served to determine the level of protein expression with immunohistochemical (IHC) staining and commercial antibodies. LOH at the CDKN2A locus was observed in 55.35% of the informative cases. Aberrant methylation was found in 37.5% and a decreased level of protein expression observed in 45% of all informative cases. Whenever P16 expression was decreased, LOH and promoter hypermethylation at CDKN2A were observed with a frequency of 73.33% and 80.95%, respectively (Fisher's test, P<0.005). Sixty-nine percent of G3 tumors had at least one genetic alteration at CDKN2A, compared with 40.9% of G1 cancers. The results indicate that CDKN2A inactivation played a significant role in the development of squamous cell carcinoma of the larynx.

Adult↗
PubMed2004

Expression of c-myc oncoprotein in laryngeal squamous cell carcinoma.

OBJECTIVE: c-myc seems to play a pivotal role in normal growth and development as well in cellular transformation and carcinogenesis. Overexpression of the c-myc oncogene has been observed in many hematopoetic and solid tumors. The role of c-myc protein in squamous cell carcinoma of the head and neck in general and laryngeal squamous cell carcinomas (LSCCs) in particular is far from clear. The aim of this study was to evaluate the relations between the level of c-myc protein in LSCCs and the clinicopathological data of patients, DNA ploidy and the SG2M phase index (PI). MATERIAL AND METHODS: The c-myc protein level was evaluated immunohistochemically in tumor specimens from 50 patients with LSCC. The DNA index and SG2M PI were determined by means of flow cytometry. RESULTS: We found c-myc protein in 34 (68%) tumors. Expression of c-myc protein was demonstrated to be frequent in nonmetastatic cases (p = 0.016). There was no association between c-myc protein level and age, primary tumor size, histological grading or type of cancer. In 13 (26%) cases we observed DNA aneuploid tumors. The mean value of the SG2M PI was 22.5%. Expression of c-myc protein was not related to SG2M PI or DNA ploidy. CONCLUSIONS: We have shown that c-myc oncoprotein may be involved in the genesis of LSCC. Our findings suggest that the detectability of c-myc protein is associated with a lower metastatic potential. The c-myc oncogene is probably not as important in laryngeal cancers compared to other cancers. Further investigations must be performed to establish the value of predicting nodal metastases in LSCC.

Aneuploidy↗
PubMed2004

Studies of the cell cycle regulatory proteins P16, cyclin D1 and retinoblastoma protein in laryngeal carcinoma tissue.

Defects in the system controlling the cell cycle can lead to an increased proliferation of cancer cells. The aim of this study was to analyse the immunohistochemical expression of chosen cell cycle proteins (P16, cyclin D1 and retinoblastoma protein) and their connection with the clinical course of the disease in laryngeal squamous cell cancer (LSCC). Cancer tissue sections obtained from 58 patients after total laryngectomy served to determine the level of the proteins' expression using immunohistochemical staining and commercial antibodies. A decreased level of P16 expression in 47 per cent, of retinoblastoma protein in 12 per cent and strong cyclin D1 expression in 48 per cent of cases was revealed. Our results show significant correlation between decreased P16 expression and increased tumour dedifferentiation. Overexpression of cyclin D1 was statistically more common in locally advanced tumours (T(3) -T(4)). Low expression of retinoblastoma protein was significantly correlated with both positive P16 immunostaining and with strong cyclin D1 expression. Our study confirms that dysfunction of cell cycle regulation is a common event and may play a significant role in the development of squamous cell carcinoma of the larynx.

Adult↗
PubMed2004

Correlation among loss of heterozygosity, promoter methylation and protein expression of MLH1 in larynx cancer.

Extensive molecular studies in development of the squamous cell carcinoma of larynx (SCCL) indicated the involvement of a variety of genes including the MLH1. To search for possible mechanism leading to MLH1 silencing in SCCL we studied LOH and promoter methylation in a homogeneous set of 62 larynx cancers. Then we evaluated immunohistochemically the MLH1 expression for 51 tumor specimens. Further, the results were correlated with microsatellite instability and subsequently with the clinical course of the disease. LOH at the MLH1 locus and aberrant methylation of its promoter were found in 47.9 and in 22.6% of tumors, respectively. A decreased expression was observed in 27.5% of the cases. MSI analysis of tumor DNA showed a microsatellite stable phenotype in 59 cases (95.2%). From our study it can be concluded that: i) molecular alterations of MLH1 play an important role in SSCL development, ii) both LOH and aberrant methylation contribute to the MLH1 inactivation in SCCL and are associated with a less advanced stage of differentiation of larynx tumors, iii) MLH1 inactivation does not lead to MSI in larynx cancer and MSI may not contribute to the development of SCCL.

Adaptor Proteins, Signal Transducing↗
PubMed2004

[DNA ploidy and DNA index in aneuploid laryngeal cancer].

UNLABELLED: Abnormal cellular DNA content, a hallmark of malignancy, is known to be an important prognostic factor in many human solid tumors. In laryngeal tumors studies are not as common as in different locations. THE PURPOSE: Of this study was to analyze the prognostic value of DNA content in squamous cell carcinoma of the larynx and relations between aneuploidy and clinical as well as histological features of the tumor. MATERIALS AND METHODS: Sixty-two patients with squamous cell carcinoma of the larynx treated in years 1997-2001 on the ENT department of the University Hospital in Wroclaw were subject of our study. Flow cytometric DNA ploidy, S-phase fraction and index DNA measurements were performed on fresh frozen tumor tissues. RESULTS: Results were compared to histological grading and TNM category. There was a positive relation between DNA aneuploidy, histological grading and with lymph node metastases. This correlation did not reach statistical significance. We also noticed low mean values of index DNA in advanced tumors (G3, T4, N+). CONCLUSIONS: The results of this study suggest that presence of aneuploid cells in laryngeal cancer can be related to lymph node metastases and tumor grading. Studies should be continued to prove results on the major number of patients.

Adult↗
PubMed2004

[S+G2M phase fraction and index of DNA in the aneuploid laryngeal cancer].

Abnormal cellular DNA content, a hallmark of malignancy, is known to be an important prognostic factor in many human solid tumors. The purpose of the following research was to analyze the prognostic value of DNA index in aneuploid squamous cell carcinoma of the larynx and relations between S+G2M phase fraction and clinical as well as histological features of the tumor. Eighty-six patients with squamous cell carcinoma of the larynx treated in years 1997-2002 in the ENT Department of the University Hospital in Wroclaw were subject of our study. Flow cytometric DNA ploidy, S-phase fraction and index DNA measurements were performed on fresh frozen tumor tissues. Results were compared to histological grading and TNM category. We have shown, there is a positive correlation between the histopathological diagnosis and DNA ploidy, between DNA ploidy and S+G2M phase fraction and between the tumor size and S+G2M phase fraction. We noticed a positive correlation between the DNA index of the tumor and presence of the nodal metastases, there was also correlation between the DNA index of the tumor and S+G2M phase fraction.

Adult↗
PubMed2004

Treatment of Reincke's edema among different professional groups: presentation of results.

OBJECTIVES: Reincke's edema is one of the most common voice disorders in adults. The main purpose of our study, was to analyze factors that may influence the development of this disease. We also estimated the results of surgical treatment. MATERIALS AND METHODS: The research was performed on a group of 261 patients with Reincke's edema treated in the ENT Department of the Medical University of Wrocław in the years 1994--2000. In the study population, women were in a majority and teachers formed the largest occupational group (30%) followed by salespersons (15%). Most of the patients (86%) were tobacco smokers. All of the patients underwent detailed videostroboscopic examination of the larynx and perceptual analysis of the voice quality before and after treatment. RESULTS: The symmetry of vocal cords before and after treatment was found in 75 patients. Lack of symmetry before treatment, and proper symmetry after surgery was observed in 71 subjects. Fifty patients showed less symmetry after treatment, and 65 patients were asymmetric before and after surgery. The difference between the symmetry of vocal folds before and after treatment was at the border of statistical significance (p = 0.069). The number of patients with full vocal cords closure increased after treatment. This difference was statistically significant (p = 0.032). The periodicity of vocal cord movements was significantly higher after treatment (p < 0.001). CONCLUSIONS: The perceptual assessment of voice, before and after treatment, revealed statistically significant post-treatment improvement in voice quality.

Adolescent↗