Search PubMed⌕ Search

Biomedical subjects

T Yamane

Publications and source records attributed to T Yamane.

At least 415 records · Page 23Linked to original sources
PubMed1989

[Acute myeloid leukemia after the treatment of Hodgkin's disease].

A 55-year-old woman developed a secondary leukemia following 1-year treatment of Hodgkin's disease. She was admitted to our hospital because of the celiac lymphadenopathy. Open laparotomy was performed. Biopsy specimens of the lymph node demonstrated Reed Sternberg cells with mature lymphocytes. She was diagnosed as having Hodgkin's disease (lymphocyte predominant type). She was treated by the combination chemotherapy consisting of mitoxantrone, cyclophosphamide, vincristine and prednisolone for Hodgkin's disease in November 1986. Hodgkin's disease achieved complete remission and she was regularly followed. No abnormal findings were observed in the peripheral blood and bone marrow. But thrombocytopenia and the blastoid cells appeared in the peripheral blood in February 1988. The bone marrow specimen was hypercellular and occupied by 90% of blastoid cells that were positive for peroxidase staining. She was diagnosed as having AML from the bone marrow aspiration and biopsy specimens. She did not respond to several chemotherapy regimens and now she is treated by low dose Ara C.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1989

[Clinical study of platinum accumulation in gastric cancer after preoperative intra-arterial injection of cisplatin].

In order to deliver a high concentration of CDDP in tumor tissue, we attempted to infuse CDDP intra-arterially for preoperative patients with resectable gastric cancer. Thirty-two patients (21 males and 11 females) were treated. The concentration of platinum was measured in cancer tissue, normal mucosa, lymph nodes without metastasis at the greater curvature. These were gathered operatively and in serum just before operation. The serum concentration of free-CDDP was also measured. Student's-t test was performed with the data. After intra-arterial injection of 60 mg CDDP, the mean concentration of platinum in cancer tissue was 1.11 +/- 0.45 microgram/g and after intravenous injection of 40 mg CDDP that was 0.30 +/- 0.11 microgram/g. After intra-arterial injection of 40 mg CDDP, the mean concentration of platinum in cancer tissue was 0.64 +/- 0.12 microgram/g, which was significantly higher than in normal gastric mucosa (0.27 +/- 0.06 micrograms/g) or serum (0.37 +/- 0.10 micrograms/ml) (p less than 0.025). The mean concentration of platinum in cancer tissue was higher than in the lymph nodes (0.45 +/- 0.10 micrograms/g), but there was no significant difference. In sera the concentrations of free-CDDP were all under the detectable limit. It was concluded that intra-arterial injection therapy of CDDP was an effective method to maintain a high concentration of CDDP in gastric cancer tissue.

Cisplatin↗
PubMed1989

[Sweet's syndrome associated with myelodysplastic syndrome].

A 49-year-old man was hospitalized because of cutaneous plaques and pancytopenia. Hematological findings, and the skin eruption suggested Sweet's syndrome associated with myelodysplastic syndrome (refractory anemia with excess of blasts; RAEB). Treatment for pancytopenia was attempted without effect. Also we tried treatment with antibiotics. The skin lesions healed and the body temperature returned to normal. This case was unusual in the association of myelodysplastic syndrome with Sweet's syndrome.

Anemia, Refractory, with Excess of Blasts↗
PubMed1988

Kinetic evaluation of conversion of phosphatidylcholine to phosphatidylethanolamine by phospholipase D from different sources.

Transphosphatidylation from phosphatidylcholine (PC) to phosphatidylethanolamine (PE) was conducted by seven phospholipase D preparations from different sources, one of which was of cabbage origin and other six of Streptomyces origin. The reactions were carried out at 30 degrees C using mixture of ethyl acetate containing PC and buffer containing ethanolamine and phospholipase D. To obtain the activity ratio of transphosphatidylation and hydrolysis at various ratios of ethanolamine and water concentrations, the apparent rate constant ratios of transphosphatidylation and hydrolysis, (k'3/k3)app, were calculated, keeping the concentration of PC constant (17.8 mM). Among the seven enzymes examined, five showed good transphosphatidylation, in which 100% of PC could be converted to PE at ethanolamine concentrations ranging from 0.3 to 1.0 M, while two showed poor transphosphatidylation activity. At higher ethanolamine concentrations, the reaction rate was decreased due to substrate inhibition. Hydrolytic reactions were conducted at 30 degrees C with respective enzymes by using mixture of ethyl acetate containing PC or PE and buffer containing phospholipase D. The ratio of (kcat/Km)PE/(kcat/Km)PC was calculated to determine the substrate specificity of various phospholipase D enzymes. The values of (k'3/k3)app varied with the origin of the enzymes, whereas the values of (kcat/Km)PE/(kcat/Km)PC were not so different. The results obtained show that (k'3/k3)app is a good parameter to select an enzyme and that the timing of stopping the reaction is also important to avoid the hydrolysis of the product.

Biological Transport↗
PubMed1988

A hydrophobic protein, chargerin II, purified from rat liver mitochondria is encoded in the unidentified reading frame A6L of mitochondrial DNA.

Previous studies showed that a hydrophobic protein called chargerin II may have a key role in energy transduction of oxidative phosphorylation, since antibody against chargerin II labeled with monoazide ethidium inhibited ATP synthesis, ATP-Pi exchange, and reversed electron flow from succinate to NAD coupled with succinate oxidation by O2. In the present work, unlabeled chargerin II was purified from intact rat liver mitochondria by high performance liquid chromatography. The purified preparation of chargerin II, which was a single protein as judged by polyacrylamide gel electrophoresis and Western blotting, was digested with lysylendopeptidase. The digest was separated on a reverse-phase column into five peptides, which all cross-reacted with the antibody against chargerin II, indicating that they were fragments of chargerin II. The sequences of two of these peptides (a total of 12 amino acids) were determined and found to be highly homologous with the sequence of the carboxyl-terminal peptide of the putative polypeptide encoded by the unidentified reading frame A6L (URFA6L) of mammalian mitochondrial DNA. The amino acid compositions of the purified preparation of chargerin II were in good accord with those of the putative product of the URFA6L. Thus, we concluded that chargerin II is encoded by the URFA6L. This is the first demonstration that the URFA6L product was identified in rat liver mitochondria and purified from the membranes.

Amino Acid Sequence↗
PubMed1988

A rapid immunostaining method for scirrhous gastric cancer during surgery using a monoclonal antibody.

In the microscopic examination of gastric cancer, it is sometimes difficult to identify scirrhous carcinoma cells, because they grow individually into the fibrous tissue. In this study we used an anti-scirrhous carcinoma monoclonal antibody S202, which was reactive with 100 per cent of the cells belonging to this type of carcinoma, in a rapid immunostaining procedure to identify the limit of tumor invasion at the time of surgery. S202 was applied using this new immunostaining method and the results compared with those obtained by the routine immunohistological method. Five minutes incubation was sufficient to stain the tumor cells with biotin-conjugated S202 and the entire procedure took only 14-15 mins. There was no difference between the new and routine methods in terms of distribution of S202-positive carcinoma cells. This rapid staining method enabled a clearer recognition of infiltrating tumor cells in the frozen sections of tissue specimens, than was previously possible. This technique will thus make it possible to determine the limit of tumor extension in resected tissues accurately and rapidly during surgery.

Adenocarcinoma, Scirrhous↗
PubMed1988

Histological diagnosis of scirrhous carcinoma of the stomach using monoclonal antibody S202.

It is sometimes difficult to identify scirrhous carcinoma cells microscopically, since individually separated malignant cells grow into the fibrous tissue without glandular formation. In this study, we used anti-scirrhous carcinoma monoclonal antibody S202, which reacted strongly with 100% of scirrhous gastric cancer samples, in order to evaluate the precise depth to which cancer cells invaded in the stomach wall. The invasive carcinoma cells could be detected more clearly by immunohistochemical staining with S202 than by histological staining with hematoxylin-eosin. Furthermore, S202 was more useful than anti-CEA (carcinoembryonic antigen) antibody.

Adenocarcinoma, Scirrhous↗
PubMed1988

Measuring relative acetylcholine receptor agonist binding by selective proton nuclear magnetic resonance relaxation experiments.

A method is presented that uses selective proton Nuclear Magnetic Resonance (NMR) relaxation measurements of nicotine in the presence of the acetylcholine receptor to obtain relative binding constants for acetylcholine, carbamylcholine, and muscarine. For receptors from Torpedo californica the results show that (a) the binding constants are in the order acetylcholine greater than nicotine greater than carbamylcholine greater than muscarine; (b) selective NMR measurements provide a rapid and direct method for monitoring both the specific and nonspecific binding of agonists to these receptors and to the lipid; (c) alpha-bungarotoxin can be used to distinguish between specific and nonspecific binding to the receptor; (d) the receptor--substrate interaction causes a large change in the selective relaxation time of the agonists even at concentrations 100x greater than that of the receptor. This last observation means that these measurements provide a rapid method to monitor drug binding when only small amounts of receptor are available. Furthermore, the binding strategies presented here may be useful for the NMR determination of the conformation of the ligand in its bound state.

Acetylcholine↗
PubMed1988

Conformation of acetylcholine bound to the nicotinic acetylcholine receptor.

We report here the biologically active conformation of acetylcholine when bound to the high-affinity state of the receptor from Torpedo californica. The acetylcholine conformation was determined in the free and bound states by proton NMR two-dimensional nuclear Overhauser effects. In agreement with x-ray crystallographic data, acetylcholine in solution has an extended conformation with an average distance between the acetyl methyl and choline methyl protons of approximately equal to 5 A. When bound to the acetylcholine receptor, acetylcholine adopts a conformation where the acetyl methyl group is close (3.3 A) to the methyl groups of the choline moiety. This bent conformation places the oxygens adjacent to one another and allows the methyl groups to form an uninterrupted hydrophobic surface over the rest of the acetylcholine molecule. The significant difference between the free- and bound-state conformations implies that structure-activity studies based solely on molecular modeling strategies must be approached with caution.

Acetylcholine↗
PubMed1988

Heterotransplantation of argyrophil small cell carcinoma of the uterine cervix integrating HPV16 DNA into nude mice.

Argyrophil small cell carcinoma of the uterine cervix (ASCC) containing human papillomavirus type 16 (HPV16) DNA has been successfully transplanted in nude mice for the first time, and we have designated the resultant cell line as YIK-1. Histology of YIK-1 was similar to that of the original tumor. The original and YIK-1 tumor cells contained argyrophil granules and neurosecretory granules in the cytoplasm, and were immunohistochemically stained positive for neuron-specific enolase, serotonin and chromogranin. Both tumors contained HPV16 DNA in a multiple-copy integrated form. Thus, YIK-1 maintains the characteristics of the original ASCC, and may therefore be useful as an animal system for experimental studies of ASCC.

Animals↗
PubMed1988

Electron microscopic localization of acridine orange chromatin interaction products in cells transformed by herpes simplex virus type 2.

The purpose of this study was to examine the distribution pattern of acridine orange (AO) binding to DNA in transformed cells and derived tumor cells. Two cell clones from hamster embryonic fibroblasts (HEF) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal HEF in culture were processed for ultracytochemistry. Uptake studies and autoradiography using [3H] thymidine and [3H]-uridine were also performed. Ultrastructural examination revealed that AO became bound to DNA exclusively within the euchromatin portion of the cell nucleus. The percentages of AO-positive cell nuclei were 18.0% and 15.5% in transformed cell clones and 21.3% and 18.3% in the derived tumor cells, but only 2. 7% in the HEF. The average amounts of AO-chromatin interaction products increased in the transformed and tumor cells. Labeling indices of [3H] thymidine were 28.9% and 33.2% in both the transformed cell clones, 32.4% and 36.9% in their tumor cells, and 3.7% in the HEF. Euchromatin/heterochromatin (EU/HEF) ratios were found to be higher in AO-positive nuclei than in AO-negative nuclei in each cell line. The increase in the number of AO-positive nuclei and in the EU/HET ratios may represent a basic reaction of cell nuclei in transformation and tumorigenesis.

Acridine Orange↗
PubMed1988

Intrahepatic ramification of the portal vein in the right and caudate lobes of the liver.

We defined the subsegmental divisions and the ramification patterns of the portal vein in the right and caudate lobes using 25 human liver casts. The ramifications of the portal vein and the subdivisions of the liver were classified based on the major portal veins with the largest diameter and those having a diameter of not less than two thirds of the largest vein in each subsegment. The following results were obtained. (1) The portal trunk showed three ramification patterns and the basic pattern was bifurcation (80%). (2) The anterior portal vein first ramified into several anterior-inferior portal veins (P5) and ran toward the superior direction to bifurcate into 2 major portal veins in the anterior-superior subsegment (S8). (3) There were three types of ramification patterns of the portal veins in S8: bifurcation (84%), trifurcation and one-pedicle type. (4) There were also three branching types of the largest vein (P5-max) in P5: ramification from the anterior portal vein, P8-anterior vein supplying the anterior region of S8 and P8-posterior vein supplying the posterior region of S8. (5) The posterior portal vein showed two ramification patterns of the bifurcation (36%) and nonbifurcation type. (6) The major portal veins in the caudate subsegment ramified at various sites such as the portal trunk, left, right and/or other portal veins.

Humans↗
PubMed1988

Proliferative response of seminal vesicle cells to androgen in mice castrated neonatally and pretreated with estrogen or androgen at adulthood.

Seminal vesicle cells of neonatally castrated adult mice show poor response to androgen, compared to those of mice castrated at adulthood; effects of pretreatment with androgen or estrogen at adulthood on androgen-induced proliferation of the seminal vesicle cells were examined in neonatally castrated mice. Male mice castrated at day 0 after birth were pretreated with daily injections of testosterone propionate (TP, 100 micrograms/mouse), 17 beta-estradiol (E2, 5 micrograms/mouse) or vehicle for 20 days starting from day 60; daily TP injections (100 micrograms/mouse) for 30 days were started again from day 110 in all the pretreated mice to examine androgen-induced proliferation by incorporation of 5-[125I]iodo-2'-deoxyuridine into the whole seminal vesicles. Both TP and E2 pretreatments significantly increased the seminal vesicle weight found before TP treatment. However, androgen-induced proliferation of the seminal vesicle found in neonatally castrated mice (poor response; long duration with a low peak on day 3) was changed at least in part to that found in mice castrated at adulthood (good response; short duration with a high peak on day 3) only following the TP pretreatment but not at all following the E2 pretreatment. The E2 pretreatment induced poor androgen-induced proliferation with a low peak on day 7.

Age Factors↗
PubMed1988

[A case of primary intrasellar malignant germ cell tumor].

A case of a 13-year-old boy with a primary, intrasellar malignant germ cell tumor is reported. An immunohistochemical study revealed that the serum HCG and AFP levels were highly elevated and that the tumor contained elements of seminoma, choriocarcinoma, a yolk sac tumor, and an embryonal carcinoma. After operation, combined chemotherapy (cisplatin, vinblastine and bleomycin, i.e. PVB therapy) and irradiation were performed in hope of achieving the radiosensitizing effect of cisplatin. As a result, partial remission was obtained. Although PVB therapy is considered effective, combined radio-chemotherapy, as was done in this case, is thought to be better method for treating an intracranial, malignant germ cell tumor.

Adolescent↗
PubMed1988

Morphology and increased growth rate of atherosclerotic intimal smooth-muscle cells.

Atherosclerotic intimal smooth-muscle cells (SMCs) in vitro showed higher growth activity than did medial SMCs obtained from either atherosclerotic or normal aortas. Using an electron microscope, it was proved in primary cultures by an explant method that intimal SMCs had rich organelles and fewer filaments in their cytoplasms. They were regarded as synthetic phenotype. In contrast, most medial SMCs had rich filaments and fewer organelles. They were regarded as contractile phenotype. When atherosclerotic intimal and normal medial SMCs were plated on type I collagen gel, cytoplasmic cyclic adenosine monophosphate concentration increased and DNA synthesis was suppressed. Intimal SMCs cultured on the gel showed contractile phenotype. Dibutyryl cyclic adenosine monophosphate added to culture media decreased DNA synthesis and altered cellular phenotype to a contractile state. Intimal SMCs were more resistant to injury by hyperlipidemic low-density liproprotein and homocysteine. Lysosomal enzyme activity was enhanced in intimal SMCs.

Animals↗